• Title/Summary/Keyword: spent medium

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Isolation and Identification of Hydrolytic Enzyme-producing Bacteria from Spent Mushroom Substrate (버섯부산물유래 가수분해효소분비 박테리아의 분리 및 동정)

  • Kim, Young-Il;Jeong, Se-Hyung;Seok, Joon-Sang;Yang, Si-Yong;Huh, Jung-Won;Kwak, Wan-Sup
    • Journal of Animal Science and Technology
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    • v.50 no.5
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    • pp.713-720
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    • 2008
  • This study was conducted to isolate and identify xylanase- and cellulase-producing thermophilic bacteria from stacked spent mushroom substrates and to determine the optimal medium conditions for their growth. Bacteria with the highest xylanase and CMCase activities were strain 3 and 201-7. Both of them were identified as Bacillus spp. and named Bacillus subtilis KU3 and Bacillus subtilis KU201-7. The optimal medium condition of Bacillus subtilis KU3 was obtained when 3%(w/v) of yeast extract and 1%(w/v) of maltose were used as nitrogen and carbon sources, respectively. That of Bacillus subtilis KU201-7 was obtained when 0.5%(w/v) of yeast extract and 0.5%(w/v) of CMC were used as nitrogen and carbon sources, respectively.

Sawdust Substitution in Growth Medium of Oyster Mushroom for Using Its By-product Spent Mushroom Substrates as Ruminant Feed (수확 후 배지의 가축 사료화를 위한 느타리 생육배지 톱밥 대체재료 선발 연구)

  • Kim, Jeong-Han;Jang, Myoung-Jun
    • The Korean Journal of Mycology
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    • v.48 no.4
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    • pp.407-414
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    • 2020
  • To replace the sawdust in the growth medium of oyster mushroom to utilize its by-product spent mushroom substrates (SMS) as feed for ruminant, we performed cultivation test using cotton seed hull pellet (CSHP), corn stalk pellet (CSP), corncob (CC), and analyzed the feed chemical properties of those SMS. As a result of cultivation test, CC and CSHP treatment took 27 days for spawn run, 4 days for primordium formation, and 3 days for development fruiting bodies, resulting in a total cultivation period of 34 days. The yield per bottle was 134 g for CC treatment, similar to 130 g for control, while CSHP treatment (112 g) and CSP treatment (68 g) were lower than that of control. The highest biological efficiency (BE) was shown in CC treatment as 80.1%, which was 11.4% higher than 68.7% of control. The SMS of CC treatment had a relatively low content of neutral detergent fiber and acid detergent fiber, and in particular, lignin content was the lowest and crude protein content was the highest among other treatments. Therefore, CC as a substitute material for sawdust was capable of stable mushroom production and excellent nutritional value as a feed for its by-products.

The Study for the Usage of Neighborhood Parks as a Festival Space in Small and Medium Sized Cities (지방 중소도시 근린공원의 축제공간적 활용에 관한 연구)

  • Park, Kyeong-Min;Jeong, Wook-Ju
    • Journal of Korean Society of Rural Planning
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    • v.19 no.3
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    • pp.183-193
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    • 2013
  • In 2011, neighborhood parks occupied a total area of $280,135,257m^2$ in small and medium sized city areas and this excluded the metropolitan regions. In the rural area, the park area per person($22.64m^2$) is larger than the urban area($9.50m^2$). It is a questionable why there is a higher park ratio in the rural area compared to the cities, where the function of the neighborhood parks is not being utilized well. But, there are already a lot of parks that has been constructed in the rural areas and a lot of money is being spent in maintaining them. Hence, rather than reducing the number of "country parks", it can be suggested that these "country parks" ought to be utilized in a different manner than the parks in the city. In that point of view, this study examined the issues of the country park usage along with the utilization of the local festivals within the parks. Firstly in this context, this study compared the amount of neighborhood parks between small and medium sized cities and metropolitan areas, and analyzed those results. Secondly, daily newspaper articles were collected and categorized to find the usage of the rural park which were then analyzed. Then, the extraction of the essential points in composing the festival space was carried out. Also, the general design guides of making the neighborhood parks were drawn. Last but not least, using these two resources, this study suggested design guidelines for using neighborhood parks as a Festival Space in small and medium sized cities.

Determination of Attenuation Collection Methods According to the Type of Radioactive Waste Drums (방사성폐기물드럼 종류별 감쇠보정방법의 결정)

  • Kwak, Sang-Soo;Choi, Byung-I1;Yoon, Suk-Jung;Lee, Ik-Whan;Kang, Duck-Won;Sung, Ki-Bang
    • Journal of Radiation Protection and Research
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    • v.22 no.4
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    • pp.309-317
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    • 1997
  • The measured radioactivity of gamma-emitting radionuclides in each radioactive waste drum using the non-destructive waste assay method is underestimated than real radioactivity in radioactive waste drum because the gamma-rays are attenuated within the medium. Therefore, the measured radioactivity should be corrected for the attenuation of gamma-rays. For the correction of the attenuation of gamma-rays, the attenuation correction method should be applied differently by considering the distribution and density of medium in radioactive wastes drum generated from nuclear power plants. In this study, the model drums were fabricated for simulating five types of radioactive waste drums generated from nuclear power plant and the optimum methods of the attenuation correction were experimentally determined to analyze the activity of radionuclides in the waste drum accurately using the segmented gamma scanning system. With the determination of the attenuation correction methods from the experimental results the transmission method and the average density method for the miscellaneous waste drum, the transmission method and the differential peak absorption method for the shielded miscellaneous waste drum were used to measure the density of medium in waste drums. Also, the average density method and the differential peak absorption method for the spent resin drum, the paraffin solidified drum, and the spent filter drum were used.

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Isolation and Identification of High Cellulolytic Bacteria from Spent Mushroom Substrate and Determination of Optimal Medium Conditions for the Growth (버섯폐배지로부터 섬유소분해력이 높은 중온성 균의 분리 및 균주생산을 위한 배지조건의 최적화)

  • Kim, Young-Il;Jung, Se-Hyung;Seok, Joon-San;Yang, Si-Yong;Huh, Jeong-Weon;Kwak, Wan-Sup
    • Microbiology and Biotechnology Letters
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    • v.35 no.3
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    • pp.255-260
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    • 2007
  • This study was conducted to isolate and identify bacteria producing xylanase and cellulase from spent mushroom substrates and to determine the optimal medium conditions for their growth. Bacteria showing high xylanase and carboxymethyl cellulase activities and low protease and amylase activities were strain 201-3 and strain 206-3. Strain 201-3 was identified as Enterobacter ludwigii and named Ent. ludwigii KU201-3. 206-3 was identified as Bacillus cereus and named B. cereus KU206-3. The optimal medium condition of Ent. ludwigii KU201-3 was obtained when 1%(w/v) of soybean meal and 3%(w/v) of sucrose were used as nitrogen and carbon source, respectively. That of B. cereus KU206-3 was obtained when 3%(w/v) of soybean meal and 1%(w/v) of molasses were used as nitrogen and carbon sources, respectively.

Post-Medium and Postproduction: Contemporaneity of Contemporary Art (포스트-미디엄과 포스트프로덕션 : 포스트모더니즘 이후 현대미술의 '동시대성(contemporaneity)')

  • Chung, Yeon Shim
    • The Journal of Art Theory & Practice
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    • no.14
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    • pp.187-215
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    • 2012
  • In recent studies of art historical methodology, such as Critical Terms for Art History and The Art of Art History, subjectivity, identity, abjection, and other terms have been placed safely in the genealogy of contemporary art history. This paper questions the contemporaneity in the story of contemporary art in our time in relation to two other critical terms that have been regularly cited by contemporary critics, not only in Euro-American fields but also in Korea. The terms are postmedium and postproduction, respectively, as used by Rosalind Krauss and Nicolas Bourriaud. This paper stems from the critical condition in which art criticism and theory have their power in the rise of neo-liberalism. But this paper does not deal with the contemporary as a chronological term for art history but rather examines the three critical terms-contemporaneity, post-medium, and postproduction-that have garnered scholarly attention. I would like to put aside postmodernism for the moment; I don't disregard the postmodern condition although the death of postmodern critical terms has resulted in the loss of its polemical power in art worlds such as in exhibitions, etc. To look at "the postproduction in the age of post-medium age after postmodernism," I first explore Krauss's notion of post-medium because, unlike media artists like Lev Manovich and Peter Weibel, Krauss's post-medium condition is different and insists on medium specificity. In this sense, Krauss has turned out to be another Greenberg in disguise. For her, photography and video are expanded mediums after Greenberg, because Krauss has spent her life explicating those mediums. Under the Cup, her recent publication, came out in 2011, and discusses her desire to defend medium-specificity against the intermedia of installation art found ubiquitously in international exhibitions and biennales. Her usage of post-medium has been taken up by Weibel as postmedia in a broader sense. But whether the post-medium condition or the postmedia age, we nonetheless enter the new age of the contemporary. Consequently, this paper questions what constitutes contemporaneity in our times. It is said that there is nothing new on earth, yet I find original artistic strategies among the younger generation in the postmedia age. The contemporary justifies its place in art fields and criticism by keeping its distance from postmodernism although we still find the remnants of postmodern artistic practices and theoretical foundations. By looking at materials written by Terry Smith, I would like to examine contemporaneity as a rhetoric where artists, critics, and curators endeavor to set up a new spirit of criticism, distant from the past of modernism and postmodernism. In discussions, modernism and postmodernism act as catalysts interacting with each other while justifying their own place. In conclusion, my paper reaches to delineate where the contemporary finds its place among artists' responses and working methods. It explores the postproduction of the Internet and the World Wide Web generations, where images become data rather than representation (of modernism) and appropriation (of postmodernism). This paper analyzes Bourriaud's text, as well as relevant artists like Pierre Huyghe, Liam Gillick, and others. By examining the aforementioned critical terms, I would like to reconsider our own contemporary art in Korea, especially among young artists influenced by digital media and the World Wide Web in the 1990s.

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Isolation of Anaerobic Bacteria from Oral Pyogenic Infections (구강 화농성 감염에서 혐기성 세균의 배양분리)

  • 장복실;이장희;최화석;최선진
    • Korean Journal of Microbiology
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    • v.23 no.1
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    • pp.43-48
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    • 1985
  • Strict anaerobic procedures and anaerobic chamber were employed in order to improve the isolation of obligate anaerobes from oral pyogenic infections. Also different culture media were utilized to maximize bacterial recovery. Two blood media: nalidixic acid Tween blood agar (NATB) and plain blood agar (BA), two non-blood media: nalidixic acid Tween agar (NAT) and Gifu anaerobic medium (GAM) were used and compared for their isolation efficacy. Specimens from seven patients were collected with syringe. After collection, the needle was sealed with sterilixed silicone rubber and brought to labortory. The time spent from specimen collection to its processing in anaerobic chamber usually was 15 min. Identification of isolated bacterial strains was done with the API-20A system. Increase in isolation of anaerobic vacteria was achieved. Obligate anaerobic bacteria isolated were 3.3 strains per specimen. This figure falls within the range of 1.9-4.4 strains per specimen reported in other countries. With respect to the media utilized, blood media were superior to non-blood media. NATB medium was effective especially for the isolation of Gram-positive cocci. A total of 15 species of Gram-negative rods was isolated and 12 of them belonged to Bacteroides.

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Activation analysis of targets and lead in a lead slowing down spectrometer system

  • Lee, Yongdeok;Kim, Jeong Dong;Ahn, Seong Kyu;Park, Chang Je
    • Nuclear Engineering and Technology
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    • v.50 no.1
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    • pp.182-189
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    • 2018
  • A neutron generation system was developed to induce fissile fission in a lead slowing down spectrometer (LSDS) system. The source neutron is one of the key factors for LSDS system work. The LSDS was developed to quantify the isotopic contents of fissile materials in spent nuclear fuel and recycled fuel. The source neutron is produced at a multilayered target by the (e,${\gamma}$)(${\gamma}$,n) reaction and slowed down at the lead medium. Activation analysis of the target materials is necessary to estimate the lifetime, durability, and safety of the target system. The CINDER90 code was used for the activation analysis, and it can involve three-dimensional geometry, position dependent neutron flux, and multigroup cross-section libraries. Several sensitivity calculations for a metal target with different geometries, materials, and coolants were done to achieve a high neutron generation rate and a low activation characteristic. Based on the results of the activation analysis, tantalum was chosen as a target material due to its better activation characteristics, and helium gas was suggested as a coolant. In addition, activation in a lead medium was performed. After a distance of 55 cm from the lead surface to the neutron incidence, the neutron intensity dramatically decreased; this result indicates very low activation.

Near-Field Transport of Radionuclide Decay Chains (방사성 핵종 붕괴 사슬의 Near-Field 이동)

  • Kang, Chul-Hyung
    • Nuclear Engineering and Technology
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    • v.26 no.2
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    • pp.277-284
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    • 1994
  • Much attention has been given to predict the near-field mass transfer of a single radioactive species from a waste solid into surrounding porous medium. But only limited considerations have been given to predict the coupled mass transfer of species with a radioactive decay chain. In this study we present an analysis assuming that the members of a decay chain dissolve congruently with a solubility-limited matrix. We give general, non-recursive analytic solutions for the transport of a radioactive decay chain in a finite porous medium when nuclides are released congruently with the matrix. As an illustration we consider the decay chain $^{234}$ Ulongrightarrow$^{230}$ Thlongrightarrow$^{226}$ Ra from spent fuel. These solutions may be useful and potentially important in performance assessment of radioactive waste repositories.

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Reference Spent Nuclear Fuel for Pyroprocessing Facility Design (파이로공정 시설 개념설계를 위한 기준 사용후핵연료 선정)

  • Cho, Dong-Keun;Yoon, Seok-Kyun;Choi, Heui-Joo;Choi, Jong-Won;Ko, Won-Il
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.6 no.3
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    • pp.225-232
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    • 2008
  • An estimation has been made for inventories and characteristics of spent nuclear fuel(SNF) to be generated from existing and planned nuclear power plants based on the 3rd Basic Plan for Electric Power Demand and Supply. The characteristics under consideration in this study are dimensions, a fuel rod array, a weight, $^{235}U$ enrichment, and the discharge burnup in terms of fuel assembly. These are essentially needed for designing a pyroprocessing facility. It is appeared that the anticipated quantity by the end of 2077 is about 23,000 tU for PWR spent nuclear fuel. It is revealed that the proportion of SNF with the initial $^{235}U$ enrichment below 4.5 weight percent(wt.%) is approximately 95 % in total. For SNF with 16$\times$16 fuel rod array the proportion is expected approximately 74% in total. It appears that the average burnup of SNF will be 55 GWd/tU after the medium and/or latter part of 2010s while the average burnup is 45 GWd/tU at present. Finally, a requirement in terms of reference SNF for designing the pyroprocessing facility has been derived from the above-mentioned results. The anticipated SNF seems to be 16$\times$16 Korean Standard Fuel Assembly with a cross section of 21.4 cm$\times$21.4 cm, a length of 453 cm, a mass of 672 kg, the initial $^{235}U$ enrichment of 4.5 wt.%, and the discharge burnup of 55 GWd/tU.

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