• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.108-109
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• 2013

Mitochondria play key roles in the production of cell's energy. Their dominant function is the synthesis of adenosine 5'-triphosphate (ATP) from adenosine diphosphate (ADP) and phosphate (Pi) through the oxidative phosphorylation. Evaluation of drug-induced mitochondrial toxicity has become increasingly important since mitochondrial dysfunction has recently been implicated in numerous diseases including cancer and diabetes mellitus. Mitochondrial functions have been monitored via oxygen consumption, mitochondrial membrane potential, and more importantly via ATP synthesis since ATP synthesis is the most essential function of mitochondria. Various analytical methods have been employed to investigate ATP synthesis in mitochondria, including high performance liquid chromatography (HPLC), bioluminescence technique, and pH measurement. However, most of these methods are based on destructive analysis or indirect monitoring through the enzymatic reaction. Infrared absorption spectroscopy (IRAS) is one of the useful techniques for real-time, label-free, and direct monitoring of biological reactions [1,2]. However, the strong water absorption requires very short path length in the order of several micrometers. Transmission measurements with thin path length are not suitable for mitochondrial assays because solution handlings necessary for evaluating mitochondrial toxicity, such as rapid mixing of drugs and oxygen supply, are difficult in such a narrow space. On the other hand, IRAS in the multiple internal reflection (MIR) geometry provides an ideal optical configuration to combine solution handling and aqueous-phase measurement. We have recently reportedon a real-time monitoring of drug-induced necrotic and apoptotic cell death using MIR-IRAS [3,4]. Clear discrimination between viable and damaged cells has been demonstrated, showing a promise as a label-free and real-time detection for cell-based assays. In the present study, we have applied our MIR-IRAS system to mitochondria-based assays by monitoring ATP synthesis in isolated mitochondria from rat livers. Mitochondrial ATP synthesis and hydrolysis were in situ monitored with MIR-IRAS, while dissolved oxygen level and solution pH were simultaneously monitored with O2 and pH electrodes, respectively. It is demonstrated that ATP synthesis and hydrolysis can be monitored by the IR spectral changes in phosphate groups in adenine nucleotides and MIR-IRAS is useful for evaluating time-dependent drug effects of mitochondrial toxicants.

• Journal of Biomedical Engineering Research
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• v.18 no.4
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• pp.429-438
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• 1997

Mechanical valve is one of the most widely used implantable artificial organs of which the reliability is so important that its failure means the death of patient. Therefore early noninvasive detection is essentially required, though mechanical valve failure with thrombosis is the most common. The objective of this paper is to detect the thrombosis formation by spectral analysis and neural network. Using microphone and amplifier, we measured the sound from the mechanical valve which is attached to the pneumatic ventricular assist device. The sound was sampled by A/D converter(DaqBook 100) and the periodogram is the main algorithm for obtaining spectrum. We made the thrombosis models using pellethane and silicon and they are thrombosis model on the valvular disk, around the sewing ring and fibrous tissue growth across the orifice of valve. The performance of the measurment system was tested firstly using 1 KHz sinusoidal wave. The measurement system detected well 1KHz spectrum as expected. The spectrum of normal and 5 kinds of thrombotic valve were obtained and primary and secondary peak appeared in each spectrum waveform. We find that the secondary peak changes according to the thrombosis model. So to distinguish the secondary peak of normal and thrombotic valve quantatively, 3 layer back propagation neural network, which contains 7, 000 input node, 20 hidden layer and 1 output was employed The trained neural network can distinguish normal and valve with more than 90% probability. As a conclusion, the noninvasive monitoring of implanted mechanical valve is possible by analysing the acoustical spectrum using neural network algorithm and this method will be applied to the performance evaluation of other implantable artificial organs.

• Journal of Wetlands Research
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• v.8 no.3
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• pp.29-38
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• 2006

Digital Elevation Model (DEM) of intertidal flat can be widely used not only for scientific fields, coastal management, fisheries, ocean safety, military, but also for understanding natural and artificial topographic changes of the tidal flat. In this study, we generated DEM of the Ganghwado southern intertidal flat, the largest tidal flat in the west coast of the Korean Peninsula, using waterline method and interferometric synthetic aperture radar (InSAR). Constructed DEM which applied waterline method to the Landsat-5 TM and Landsat-7 ETM+ images closely expresses overall topographic relief of tidal flat. We found that the accuracy was determined by the number of waterlines which reflect various tidal conditions. The application of InSAR to the ERS-1/2 and ENVISAT images showed that only ERS-1/2 tandem pairs successfully generated DEM in the part of northern Yeongjongdo, but construction of DEM in the other areas was difficult due to the low coherence caused by a lot of surface remnant waters. In the near future, Kompsat-2 will provide satellite images having multi-spectral and high spatial resolution within a relatively short period at different sea levels. Application of waterline method to these images will help us construct a high precision tidal flat DEM. Also, we should develop DEM generation method using single-pass microwave satellite images.

• Korean Journal of Remote Sensing
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• v.24 no.4
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• pp.289-298
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• 2008

An algorithm was developed to retrieve both cloud optical thickness and effective particle radius considered the aerosol effect on clouds. This study apply the algorithm of Nakajima and Nakajima (1995) that is used to retrieve cloud optical thickness and effective particle radius from visible, near infrared satellite spectral measurements. To retrieve cloud properties, Look-up table (LUT) was made under different atmospheric conditions by using a radiative transfer model. Especially the vertical distribution of aerosol is based on a tropospheric aerosol profile in radiative transfer model. In the case study, we selected the extensive forest fire occurred in Russia in May 2003. The aerosol released from this fire may be transported to Korea. Cloud properties obtained from these distinct atmospheric situations are analysed in terms of their possible changes due to the interactions of the clouds with the aerosol particle plumes. Cloud properties over the East sea at this time was retrieved using new algorithm. The algorithm is applied to measurements from the MODerate Resolution Imaging Spectrometer (MODIS) onboard the Terra spacecrafts. As a result, cloud effective particle radius was decreased and cloud optical thickness was increased during aerosol event. Specially, cloud effective particle radius is hardly greater than $20{\mu}m$ when aerosol particles were present over the East Sea. Clouds developing in the aerosol event tend to have more numerous but smaller droplets.

• Korean Journal of Remote Sensing
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• v.34 no.6_2
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• pp.1203-1213
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• 2018

In Arctic and Antarctic ocean, remote sensing is the most effective observation for environmental changes due to the inaccessibility of the regions. Even though satellite, UAV (Unmanned Aerial Vehical) are well known remote sensing platforms, and research vessel also used for automatic measurement on the regions, varied environment of Polar regions require time series and wide coverage of data. Especially, in high latitude, apply an optical satellite remote sensing is not easy due to low sun altitude. In this paper, we introduce an operation of hyper-spectrometer (HyperSAS/Satlantic inc.) which is mounted on Ice Breaker Research Vessel ARAON of Korea Polar Research Institute since 2010, to acquire an above water reflectance atomatically through every research cruise on Arctic and Antarctic ocean and transit both regions. In addition to, auxiliary data for the remotely acquired data, in situ water sampling were also obtained. The above water reflectance and in situ water sampling data are continuously acquired since 2010 will contribute to improve an Ocean Color algorithm in the high latitude and help to understand ocean reflectances over from high latitude through low latitude. Preliminary result from above water reflectance showed characteristics of Arctic ocean and Antarctic Ocean and used to develop algorithms for estimating various ocean factors such as chlorophyll and suspended sediment.

• Korean Journal of Remote Sensing
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• v.38 no.6_1
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• pp.1343-1356
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• 2022

Clouds or shadows are the most problematic when monitoring crops using optical satellite images. To reduce this effect, a composite algorithm was used to select the maximum Normalized Difference Vegetation Index (NDVI) for a certain period. This Maximum NDVI Composite (MNC) method reduces the influence of clouds, but since only the maximum NDVI value is used for a certain period, it is difficult to show the phenomenon immediately when the NDVI decreases. As a way to maintain the spectral information of crop as much as possible while minimizing the influence of clouds, a Score-Based Composite (SBC) algorithm was proposed, which is a method of selecting the most suitable pixels by defining various environmental factors and assigning scores to them when compositing. In this study, the Sentinel-2A/B Level 2A reflectance image and cloud, shadow, Aerosol Optical Thickness(AOT), obtainging date, sensor zenith angle provided as additional information were used for the SBC algorithm. As a result of applying the SBC algorithm with a 15-day and a monthly period for Dangjin rice fields and Taebaek highland cabbage fields in 2021, the 15-day period composited data showed faster detailed changes in NDVI than the monthly composited results, except for the rainy season affected by clouds. In certain images, a spatially heterogeneous part is seen due to partial date-by-date differences in the composited NDVI image, which is considered to be due to the inaccuracy of the cloud and shadow information used. In the future, we plan to improve the accuracy of input information and perform quantitative comparison with MNC-based composite algorithm.

• Journal of Life Science
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• v.33 no.8
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• pp.639-650
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• 2023

The levels of harmful components in aerosols from heated tobacco products (HTPs) have been reported to be significantly lower than in cigarette smoke. However, it remains unclear whether the use of HTPs can mitigate the cardiovascular risks associated with cigarette smoking (CS). The objective of this study was to investigate the effects of a short-term switch from CS to HTP use on cardiac autonomic regulation (CAR). Seven healthy male smokers completed an open-label, randomized, cross-over trial consisting of five days of CS, use of three different HTPs (IQOS use, IQ; lil SOLID use, LS; lil HYBRID use, LH), or non-smoking (NS). Each session was separated by a one-week washout period, and levels of exhaled carbon monoxide (CO) and carboxyhemoglobin (COHb), systolic (SBP) and diastolic blood pressure (DBP), and heart rate variability (HRV) reflecting CAR were assessed before use of the product assigned to each session and at 24, 48, 72, 96, and 120 hr after use. Levels of exhaled CO and COHb were statistically significantly reduced only during NS. There were no statistical changes in SBP and DBP within any session. However, in HRV spectral analysis, log-transformed high frequency (lnHF) increased statistically significantly in IQ, LS, and NS, respectively. Normalized HF (HFnu) was significantly increased in NS and LH, respectively. lnHF and HFnu showed significant interaction effects. The findings of this study suggest that a short-term switch to HTPs instead of CS may lead to different distribution patterns of CAR, primarily driven by enhanced cardiac vagal tone.

• ALGAE
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• v.38 no.2
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• pp.141-150
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• 2023

Light quality is a common environmental factor which influences the metabolism of biochemical substances in algae and leads to the response of algal growth and development. Pyropia yezoensis is a kind of economic macroalgae that naturally grows in the intertidal zone where the light environment changes dramatically. In the present study, P. yezoensis thalli were treated under white light (control) and monochromatic lights with primary colors (blue, green, and red) for 14 days to explore their physiological response to light quality. During the first 3 days of treatment, P. yezoensis grew faster under blue light than other light qualities. In the next 11 days, it showed better adaptation to green light, with higher growth rate and photosynthetic capacity (reflected by a higher rETR_max = 61.58 and E_k = 237.78). A higher non-photochemical quenching was observed in the treatment of red light than others for 14 days. Furthermore, the response of P. yezoensis to light quality also results in the difference of photosynthetic pigment contents. The monochromatic light could reduce the synthesis of all pigments, but the reduction degree was different, which may relate to the spectral absorption characteristics of pigments. It was speculated that P. yezoensis adapted to a specific or changing light environments by regulating the synthesis of pigments to achieve the best use of light energy in photosynthesis and premium growth and metabolism.

• Journal of The Geomorphological Association of Korea
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• v.27 no.1
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• pp.1-19
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• 2020

A river bed which is submerged in water at high flow and becomes part of the river at low flow, serves as a bridge between the river and the land. The channel bar creates a unique ecosystem with vegetation adapted to the particular environment and the water pool forms a wetland that plays a very important role in the environment. To evaluate anthropogenic impacts on the river bed in the Middle Yeongsangang River, the fluvial landforms in the stream channel were analyzed using multi-temporal remotely-sensed images. In the aerial photograph of 2005 taken before the construction of the large weirs, oxbow lakes, mid-channel bars, point bars, and natural wetlands between the artificial levees were identified. Multiple bars divided the flow of stream water to cause the braided pattern in a particular section. After the construction of the Seungchon weir, aerial photographs of 2013 and 2015 revealed that most of the fluvial landforms disappeared due to the dredging of its riverbed and water level control(maintenance at 7.5El.m). Sentinel-2 images were analyzed to identify differences between before and after the opening of weir gate. Change detection was performed with the near infrared and shortwave infrared spectral bands to effectively distinguish water surfaces from land. As a result, water surface area of the main stream of the Yeongsangang River decreased by 40% from 1.144km² to 0.692km². A large mid-channel bar that has been deposited upstream of the weir was exposed during low water levels, which shows the obvious influence of weir on the river bed. Newly formed unvegetated point bars that were deposited on the inside of a meander bend were identified from the remotely sensed images. As the maintenance period of the weir gate opening was extended, various habitats were created by creating pools and riffles around the channel bars. Considering the ecological and hydrological functions of the river bed, it is expected that the increase in bar areas through weir gate opening will reduce the artificial interference effect of the weir.

• Korean Journal of Agricultural and Forest Meteorology
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• v.25 no.4
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• pp.245-257
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• 2023

The representative crop in the Republic of Korea, rice, is cultivated over extensive areas every year, which resulting in reduced resistance to pests and diseases. One of the major rice diseases, rice blast disease, can lead to a significant decrease in yields when it occurs on a large scale, necessitating early detection and effective control of rice blast disease. Drone-based crop monitoring techniques are valuable for detecting abnormal growth, but frequent image capture for potential rice blast disease occurrences can consume significant labor and resources. The purpose of this study is to early detect rice blast disease using remote sensing data, such as drone and satellite images, along with weather data. Satellite images was helpful in identifying rice cultivation fields. Effective detection of paddy fields was achieved by utilizing vegetation and water indices. Subsequently, air temperature, relative humidity, and number of rainy days were used to calculate the risk of rice blast disease occurrence. An increase in the risk of disease occurrence implies a higher likelihood of disease development, and drone measurements perform at this time. Spectral reflectance changes in the red and near-infrared wavelength regions were observed at the locations where rice blast disease occurred. Clusters with low vegetation index values were observed at locations where rice blast disease occurred, and the time series data for drone images allowed for tracking the spread of the disease from these points. Finally, drone images captured before harvesting was used to generate spatial information on the incidence of rice blast disease in each field.