• Title/Summary/Keyword: spectra classification

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Mastitis Diagnostics by Near-infrared Spectra of Cows milk, Blood and Urine Using SIMCA Classification

  • Tsenkova, Roumiana;Atanassova, Stefka
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1247-1247
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    • 2001
  • Constituents of animal biofluids such as milk, blood and urine contain information specifically related to metabolic and health status of the ruminant animals. Some changes in composition of biofluids can be attributed to disease response of the animals. Mastitis is a major problem for the global dairy industry and causes substantial economic losses from decreasing milk production and reducing milk quality. The purpose of this study was to investigate potential of NIRS combined with multivariate analysis for cow's mastitis diagnosis based on NIR spectra of milk, blood and urine. A total of 112 bulk milk, urine and blood samples from 4 Holstein cows were analyzed. The milk samples were collected from morning milking. The urine samples were collected before morning milking and stored at -35$^{\circ}C$ until spectral analysis. The blood samples were collected before morning milking using a catheter inserted into the carotid vein. Heparin was added to blood samples to prevent coagulation. All milk samples were analyzed for somatic cell count (SCC). The SCC content in milk was used as indicator of mastitis and as quantitative parameter for respective urine and blood samples collected at same time. NIR spectra of blood and milk samples were obtained by InfraAlyzer 500 spectrophotometer, using a transflectance mode. NIR spectra of urine samples were obtained by NIR System 6500 spectrophotometer, using 1 mm sample thickness. All samples were divided into calibration set and test set. Class variable was assigned for each sample as follow: healthy (class 1) and mastitic (class 2), based on milk SCC content. SIMCA was implemented to create models of the respective classes based on NIR spectra of milk, blood or urine. For the calibration set of samples, SIMCA models (model for samples from healthy cows and model for samples from mastitic cows), correctly classified from 97.33 to 98.67% of milk samples, from 97.33 to 98.61% of urine samples and from 96.00 to 94.67% of blood samples. From samples in the test set, the percent of correctly classified samples varied from 70.27 to 89.19, depending mainly on spectral data pretreatment. The best results for all data sets were obtained when first derivative spectral data pretreatment was used. The incorrect classified samples were 5 from milk samples,5 and 4 from urine and blood samples, respectively. The analysis of changes in the loading of first PC factor for group of samples from healthy cows and group of samples from mastitic cows showed, that separation between classes was indirect and based on influence of mastitis on the milk, blood and urine components. Results from the present investigation showed that the changes that occur when a cow gets mastitis influence her milk, urine and blood spectra in a specific way. SIMCA allowed extraction of available spectral information from the milk, urine and blood spectra connected with mastitis. The obtained results could be used for development of a new method for mastitis detection.

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Development of Earthquake Prevention Technique Considering Geotechnical Site Characteristics of Korea (국내 지반조건이 고려된 지진 방재기술 확립 방안;지반분류 방법 개선 방안을 중심으로)

  • Kim, Dong-Soo;Yoon, Jong-Ku;Kim, Kyung-Teak;Cho, Seong-Ha
    • Proceedings of the Korean Geotechical Society Conference
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    • 2005.10a
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    • pp.154-162
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    • 2005
  • In this paper, site response analyses were performed based on equivalent linear technique using the shear wave velocity profiles of 162 sites collected around the Korean peninsula. The site characteristics, particularly the shear wave velocities and the depth to the bedrock, are compared to those in the western United States. The results show that the site-response coefficients based on the mean shear velocity of the top 30m ($V_{S30}$) suggested in the current code underestimates the motion in short-period ranges and overestimates the motion in mid-period ranges. Also the current Korean code based on UBC is required to be modified considering site characteristics in Korea for the reliable estimation of site amplification. From the results of numerical estimations, new regression curves were derived between site coefficients ($F_a$ and $F_v$) and the fundamental site periods, and site coefficients were grouped based on site periods in the regions of shallow bedrock. The standard deviations of the proposed method was reasonable compared to site classification based on $V_{S30}$. Finally, new site classification system is recommended based on site periods for regions of shallow bedrock depth in Korea.

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A COMPARISON OF OBJECTED-ORIENTED AND PIXELBASED CLASSIFICATION METHODS FOR FUEL TYPE MAP USING HYPERION IMAGERY

  • Yoon, Yeo-Sang;Kim, Yong-Seung
    • Proceedings of the KSRS Conference
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    • v.1
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    • pp.297-300
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    • 2006
  • The knowledge of fuel load and composition is important for planning and managing the fire hazard and risk. However, fuel mapping is extremely difficult because fuel properties vary at spatial scales, change depending on the seasonal situations and are affected by the surrounding environment. Remote sensing has potential of reduction the uncertainty in mapping fuels and offers the best approach for improving our abilities. This paper compared the results of object-oriented classification to a pixel-based classification for fuel type map derived from Hyperion hyperspectral data that could be enable to provide this information and allow a differentiation of material due to their typical spectra. Our methodological approach for fuel type map is characterized by the result of the spectral mixture analysis (SMA) that can used to model the spectral variability in multi- or hyperspectral images and to relate the results to the physical abundance of surface constitutes represented by the spectral endmembers. Object-oriented approach was based on segment based endmember selection, while pixel-based method used standard SMA. To validate and compare, we used true-color high resolution orthoimagery

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Modification of Site Classification System for Amplification Factors considering Geotechnical Conditions in Korea (국내 지반 특성에 따른 합리적 증폭 계수의 결정을 위한 지반 분류 체계 개선 방안 고찰)

  • Sun, Chang-Guk;Chung, Choong-Ki;Kim, Dong-Soo
    • Proceedings of the Earthquake Engineering Society of Korea Conference
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    • 2005.03a
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    • pp.90-101
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    • 2005
  • For the site characterization at two representative inland areas, Gyeongju and Hongsung, in Korea, in-situ seismic tests containing boring investigations and resonant column tests were performed and site-specific ground response analyses were conducted using equivalent linear as well as nonlinear scheme. The soil deposits in Korea were shallower and stiffer than those in the western US, from which the site classification system and site coefficients in Korea were derived. Most sites were categorized as site classes C and D based on the mean shear wave velocity to 30 m, Vs30 ranging between 250 and 650 m/s. Based on the acceleration response spectra determined from the site-specific analyses, the site coefficients specified in the Korean seismic design guide underestimate the ground motion in the short-period band and overestimate the ground motion in mid-period band. These differences can be explained by the differences in the bedrock depth and the soil stiffness profile between Korea and western US. The site coefficients were re-evaluated and the preliminary site classification system was introduced accounting for the local geologic conditions on the Korean peninsula.

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Surface-Engineered Graphene surface-enhanced Raman scattering Platform with Machine-learning Enabled Classification of Mixed Analytes

  • Jae Hee Cho;Garam Bae;Ki-Seok An
    • Journal of Sensor Science and Technology
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    • v.33 no.3
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    • pp.139-146
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    • 2024
  • Surface-enhanced Raman scattering (SERS) enables the detection of various types of π-conjugated biological and chemical molecules owing to its exceptional sensitivity in obtaining unique spectra, offering nondestructive classification capabilities for target analytes. Herein, we demonstrate an innovative strategy that provides significant machine learning (ML)-enabled predictive SERS platforms through surface-engineered graphene via complementary hybridization with Au nanoparticles (NPs). The hybridized Au NPs/graphene SERS platforms showed exceptional sensitivity (10-7 M) due to the collaborative strong correlation between the localized electromagnetic effect and the enhanced chemical bonding reactivity. The chemical and physical properties of the demonstrated SERS platform were systematically investigated using microscopy and spectroscopic analysis. Furthermore, an innovative strategy employing ML is proposed to predict various analytes based on a featured Raman spectral database. Using a customized data-preprocessing algorithm, the feature data for ML were extracted from the Raman peak characteristic information, such as intensity, position, and width, from the SERS spectrum data. Additionally, sophisticated evaluations of various types of ML classification models were conducted using k-fold cross-validation (k = 5), showing 99% prediction accuracy.

Nondestructive Classification of Viable and Non-viable Radish (Raphanus sativus L) Seeds using Hyperspectral Reflectance Imaging (초분광 반사광 영상을 이용한 무(Raphanus sativus L) 종자의 발아와 불발아 비파괴 판별)

  • Ahn, Chi Kook;Mo, Chang Yeun;Kang, Jum-Soon;Cho, Byoung-Kwan
    • Journal of Biosystems Engineering
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    • v.37 no.6
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    • pp.411-419
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    • 2012
  • Purpose: Nondestructive evaluation of seed viability is a highly demanded technique in the seed industry. In this study, hyperspectral imaging system was used for discrimination of viable and non-viable radish seeds. Method: The spectral data with the range from 400 to 1000 nm measured by hyperspectral reflectance imaging system were used. A calibration and a test models were developed by partial least square discrimination analysis (PLS-DA) for classification of viable and non-viable radish seeds. Either each data set of visible (400~750 nm) and NIR (750~1000 nm) spectra and the spectra of the combined spectral ranges were used for developing models. Results: The discrimination accuracy of calibration was 84% for visible range and 76.3% for NIR range. The discrimination accuracy of test was 84.2% for visible range and 75.8% for NIR range. The discrimination accuracies of calibration and test with full range were 92.2% and 92.5%, respectively. The resultant images based on the optimal PLS-DA model showed high performance for the discrimination of the nonviable seeds from the viable seeds with the accuracy of 95%. Conclusions: The results showed that hyperspectral reflectance imaging has good potential for discriminating nonviable radish seeds from massive amounts of viable seeds.

Machine Vision Instrument to Measure Spray Droplet Sizes (기계시각을 이용한 분무입자크기 측정)

  • Jeon, Hong-Young;Tian, Lei
    • Journal of Biosystems Engineering
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    • v.35 no.6
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    • pp.443-449
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    • 2010
  • A machine vision-based instrument to measure a droplet size spectrum of a spray nozzle was developed and tested to evaluate its accuracy on measuring spray droplet sizes and classifying nozzle sizes. The instrument consisted of a machine vision, light emitting diode (LED) illumination and a desktop computer. The illumination and machine vision were controlled by the computer through a C++ program. The program controlled the machine vision to capture droplet images under controlled illumination, and processed the droplet images to characterize the droplet size distribution of a spray nozzle. An image processing algorithm was developed to improve the accuracy of the system by eliminating random noise and out-of-focus droplets in droplet images while measuring droplet sizes. The instrument measured sizes of the three different balls (254.0, 497.8 and $793.8\;{\mu}m$) and the measurement ranges were $241.2-273.6\;{\mu}m$, $492.9-529.6\;{\mu}m$ and $800.8-824.1\;{\mu}m$ for 254.0-, 497.84- and $793.75-\;{\mu}m$ balls, respectively. Error of the measured droplet mean was less than 3.0 %. Droplet statistics, $D_{V0.1}$, $D_{V0.5}$ and $D_{V0.9}$, of a reference nozzle set were measured, and droplet size spectra of five spray nozzles covering from very fine to extremely coarse were measured to classify spray nozzle sizes. Ninety percent of the classification results of the instrument agreed with manufacturer's classification. A comparison study was carried out between developed and commercial instruments, and measurement results of the developed instrument were within 20 % of commercial instrument results.

Classification of Red Wines by Near Infrared Transflectance Spectroscopy

  • W.Guggenbichler;Huck, C.W.;M.Popp;G.K.Bonn
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1516-1516
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    • 2001
  • During the recent years, wine analysis has played an increasing role due the health benefits of phenolic ingredients in red wine [1]. On the other hand there is the need to be able to distinguish between different wine varieties. Consumers want to know if a wine is an adulterated one or if it is based on the pure grape. Producers need to certificate their wines in order to ensure compliance with legal regulations. Up to now, the attempts to investigate the origin of wines were based on high-performance liquid chromatography (HPLC), gas chromatography (GC) and pyrolysis mass spectrometry (PMS) [l,2,3]. These methods need sample pretreatment, long analysis times and therefore lack of high sample throughput. In contradiction to these techniques using near infrared spectroscopy (NIRS), no sample pretreatment is necessary and the analysis time for one sample is only about 10 seconds. Hence, a near infrared spectroscopic method is presented that allows a fast classification of wine varieties in bottled red wines. For this, the spectra of 50 bottles of Cabernet Sauvignon, Lagrein and Sangiovese (Chianti) were recorded without any sample pretreatment over a wavelength range from 1000 to 2500 nm with a resolution of 12 cm$\^$-1/. 10 scans were used for an average spectrum. In order to yield best reproducibility, wines were thermostated at 23$^{\circ}C$ and a optical layer thickness of 3 mm was used. All recorded spectra were partitioned into a calibration and validation set (70% and 30%). Finally, a 3d scatter plot of the different investigated varieties allowed to distinguish between Cabernet Sauvignon, Lagrein and Sangiovese (Chianti). Considering the short analysis times this NRS-method will be an interesting tool for the quality control of wine verification and also for experienced sommeliers.

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A Study on the Hyperspectral Image Classification with the Iterative Self-Organizing Unsupervised Spectral Angle Classification (반복최적화 무감독 분광각 분류 기법을 이용한 하이퍼스펙트럴 영상 분류에 관한 연구)

  • Jo Hyun-Gee;Kim Dae-Sung;Yu Ki-Yun;Kim Yong-Il
    • Korean Journal of Remote Sensing
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    • v.22 no.2
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    • pp.111-121
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    • 2006
  • The classification using spectral angle is a new approach based on the fact that the spectra of the same type of surface objects in RS data are approximately linearly scaled variations of one another due to atmospheric and topographic effects. There are many researches on the unsupervised classification using spectral angle recently. Nevertheless, there are only a few which consider the characteristics of Hyperspectral data. On this study, we propose the ISOMUSAC(Iterative Self-Organizing Modified Unsupervised Spectral Angle Classification) which can supplement the defects of previous unsupervised spectral angle classification. ISOMUSAC uses the Angle Division for the selection of seed points and calculates the center of clusters using spectral angle. In addition, ISOMUSAC perform the iterative merging and splitting clusters. As a result, the proposed algorithm can reduce the time of processing and generate better classification result than previous unsupervised classification algorithms by visual and quantitative analysis. For the comparison with previous unsupervised spectral angle classification by quantitative analysis, we propose Validity Index using spectral angle.

Development of Site Classification System and Modification of Design Response Spectra considering Geotechnical Site Characteristics in Korea (I) - Problem Statements of the Current Seismic Design Code (국내 지반특성에 적합한 지반분류 방법 및 설계응답스펙트럼 개선에 대한 연구 (I) - 국내 내진설계기준의 문제점 분석)

  • Yoon, Jong-Ku;Kim, Dong-Soo;Bang, Eun-Seok
    • Journal of the Earthquake Engineering Society of Korea
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    • v.10 no.2 s.48
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    • pp.39-50
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    • 2006
  • Site response analyses were peformed based on equivalent linear technique using the shear wave velocity profiles of 162 sites collected around the Korean Peninsula. The she characteristics, particularly the shear wave velocities and the depth to bedrock, are compared to those in the western United States. The site coefficients of short period $(F_a)$ and the long period $(F_v)$ obtained from this study were significantly different compared to 1997 Uniform Building Code (1997 UBC). $F_a$ underestimated the motion in shot period ranges and $F_v$ overestimated the motion in mid period ranges in Korean seismic guideline. It is found that the existing Korean seismic design code were is required to be modified considering geological site conditions in Korea for the reliable estimation of sue amplification. Problems of the current seismic design code were dicussed in this paper and the development of site classification method and modification of desing response spectra were discussed in the companion papers(II-Development of Site Classification System and III-Modification of Dosing Response Specra).