• 복식
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• 제63권6호
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• pp.14-28
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• 2013

This study aims to identify the elements of black culture and use it to analyze the formative characteristics of casual fashion items as well as the symbolic meanings presented in the graphic designs of the items. First of all, in order to distinguish the identity of black culture, this study made a division into racial, social, and artistic aspects based on cultural specificities of Stuart Hall, a culture theorist. This was for a theoretical review and based on this review, a theoretical framework was developed to analyze the characteristics of American casual fashion design of black people. The case study selected 1719 fashion images of 137 male brands and 33 female brands from websites of companies manufacturing casual wear of black people in America. The following is a summary of the study results. The conceptual characteristics based on specificities of black culture can be categorized into ten attributes: playfulness in order to digest human grief and emotion positively, satire of the mainstream society, spontaneity through emotional expression from the heart, abstractness of various emotions elating suffering, humor trying to express negative aspects as the meaning of light and innocent laughter, dependence with passive attitudes toward discrimination and master-slave relationships in history, resistance against discrimination and disadvantages, African orientation and primitiveness pursuing natural conditions, aggressiveness against violence and unfair treatment, and confidence and defiance against social deprivation and corruption. Based on the results of an analysis through formative classification system founded on racial, social, and artistic aspects of black people, specificities of black culture are of formative characteristics of their casual fashion design. These elements are presented on graphic t-shirts through strong colors, loose silhouette, and details with many pockets. These characteristics were reflected in symbolic meanings presented in the graphic.

• Journal of Microbiology and Biotechnology
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• 제20권6호
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• pp.968-973
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• 2010

In order to study its biochemical properties, the integrase (IN) protein of feline foamy virus (FFV) was overexpressed in Escherichia coli, purified by two-step chromatography, (Talon column and heparin column), and characterized in biochemical aspects. For the three enzymatic reactions of the 3'-processing, strand transfer, and disintegration activities, the $Mn^{2+}$ ion was essentially required as a cofactor. Interestingly, $Co^{2+}$ and $Zn^{2+}$ ions were found to act as effective cofactors, whereas other transition elements such as $Ni^{2+}$, $Cu^{2+}$, $La^{3+}$, $Y^{3+}$, $Cd^{2+}$, $Li^{1+}$, $Ba^{2+}$, $Sr^{2+}$, and $V^{3+}$ were not. Regarding the substrate specificity, FFV IN has low substrate specificities as it cleaved in a significant level prototype foamy virus (PFV) U5 LTR substrate as well as FFV U5 LTR substrate, whereas PFV IN did not. Finally, the 3'-processing activity was observed in high concentrations of several solvents such as CHAPS, glycerol, Tween 20, and Triton X-100, which are generally used for dissolution of chemicals in inhibitor screening. Therefore, in this first report showing its biochemical properties, FFV IN is proposed to have low specificities on the use of cofactor and substrate for enzymatic reaction as compared with other retroviral INs.

• Journal of Periodontal and Implant Science
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• 제33권2호
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• pp.179-191
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• 2003

Since periodontal infections are suggested as risk factors for the development of cardiovascular diseases, the present study was performed to evaluate the T cell immune responses specific to Pophylomonas gingivalis(P. gingivalis) heat shock protein(hsp) 60 and T-cell and B-cell epitope specificities for P. gingivalis hsp60 in atherosclerosis. Anti-P, gingivalis IgG antibody titers were elevated in all patients. We could establish P. gingivalis hsp-specific T cell lines from the atheroma lesions, a mixture of $CD4^+$ and $CD8^+$ cells producing the cytokines characteristic of both Th1 and Th2 subsets. of 108 overlapping synthetic peptides spanning whole P. gingivalis hsp60 molecule, ten peptides with common epitopes specificities for both T-cell and B-cell were identified. it was concluded that P. gingivalis hsp60 might K involved in the immunoregulatory process of atherosclerotic diseases with epitope specificities.

• 생명과학회지
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• 제6권4호
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• pp.234-240
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• 1996

PKR인산화효소의 억제인자로서 밝혀진 이중선RNA결합단백질 (RBF)의 RNA결합특이성을 정기영도에 의한 RNA 이동변화실험과 여과막결합도실험에 의해 측정하였다. RBF는 바이러스RNA나 stem/loop구조를 지니는 합성 RNA들에 대한 다양한 친화력을 지니는 것으로 나타났으며 충분한 GC가 포함된 11염기쌍으로 이루어진 RNA stem helix RBF가 결합하기 위한 최소한의 RNA구조로 제시되고 있다. 자연적 RNA구조에 대한 RBF의 결합은 poly(I) : poly(C)의 첨가에 의해 반전되었으며 E. coli 5S RNA경우는 효과를 거의 나타내지 않았다.

• KSBB Journal
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• 제9권1호
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• pp.63-71
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• 1994

During the last decade enzyme reaction in organic solvent has been studied to show that specificity in buffer is different from that in organic solvent. The specificity of restriction enzyme was effected by various factors such as ionic strength, salt organic solvent and temperature. In this study, restriction enzyme PvuII which is used most frequently in genetic engineering and the substrate was vector pGEM3 whose sequence was already known were used. As a result the recognition sequence site was changed in the presence of organic solvents whose Log P are -1.5∼0. Their specificities were contrast with activities were contrasted. Specificities were not changed in organic solvent easily in inactivating enzyme. We think that the enzyme recognition site was not changed randomly but by preferential order. A recombinant vector which does not contain typical cleavage site CAG↓CTG was cleaved in 20% ethanol solution. This result might show that restriction enzyme could be used to cleave at unusual sites by changing the reaction conditions.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
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• pp.188.2-188
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• 1999

No Abstract, See Full Text

• 한국언론정보학보
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• 제66권
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• pp.184-210
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• 2014

한국 디지털사회의 형성과 발전에는 역사적으로 기술의 굴절적 토착화와 과열된 기술숭배가 자리한다. 역사적으로도 인터넷에서 '신'권위주의적 통제와 억압의 계기적 측면이 과도하게 집중화되고 있는 반면 아래로부터 이용자들의 적극적 시민적 의제 형성을 위한 자율의 움직임은 다른 어느 나라보다 정치적이다. 이 글은 적어도 1990년대 이래 이와 같은 한국의 디지털 기술의 전개 방식에 있어서 국가적 특수성을 보려 한다. 이 글에서 '기술잉여'는 한 사회의 통제 능력 이상으로 과도하게 기술들이 비정상적으로 사회에 착근되는 상황을 지칭한다. '기술잉여사회'란 바로 이와 같은 기술잉여가 누적적으로 나타난 특징적 사회 유형을 지칭한다. 이는 제도정치의 성숙도에 비해 기술과잉과 잉여에 의해 빚어지는 사회 왜곡과 비정상성이 잦은 우리 사회를 상징화한다. 이 글에서는 기술잉여사회의 특성을 유형화하고, 중국, 일본, 미국 등 정보기술 영향력의 경쟁 국가들과의 비교를 통해 한국적 기술발전의 퇴행성을 구체화한다.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2005년도 하계 학술대회 논문집
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• pp.349-352
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• 2005

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 1998년도 학술발표회
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• pp.11-11
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• 1998

Zinc fingers of Cys$_2$His$_2$ class constitute one of the most common DNA binding motifs in eukaryotes. Unlike other DNA binding motifs, zinc finger proteins recognize very diverse DNA sites, and their sequence specificities can be systematically changed by phage display.(omitted)

• Journal of Periodontal and Implant Science
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• 제33권4호
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• pp.543-553
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• 2003

Due to considerably high degree of sequence homology between bacterial and human heat shock proteins(hsp), it has been widely thought that this protein might be involved in autoimmune disease mechanisms in humans. To elucidate how stress proteins contribute in the immunopathogenesis of periodontitis, the present study was performed to evaluate the T cell immune responses specific to Porphyromonas gingivalis (P. gingivalis) heat shock protein (hsp)60 and T-cell epitope specificities for P. gingivalis hsp60 in periodontitis. Anti-P. gingivalis IgG antibody titers were elevated in all patients. We could establish P. gingivalis hsp-specific T cell ines from the peripheral blood of peridontitis, a mixture of $CD4^+$ and $CD8^+$ cells. Of 108 overlapping synthetic peptides spanning whole P. gingivalis hsp60 moleculc, ten peptides with cpitopes specifities for T-cell were showed. Interestingly, ten epitopes were also identified as T-cell epitopes in the present study as well as B-cell epitopes in peridontitis. Therefore, all the ten representative epitopes were designated as common T-and B-cell epitopes for peridontitis. It is critical in developing a peptide vaccine strategy for potential prevention of periodontitis. It was concluded that P. gingivalis hsp60 might be involved in the immunoregulatory process of periodontitis with heat shock protein specificities.