• Journal of Microbiology and Biotechnology
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• 제27권12호
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• pp.2075-2088
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• 2017

Salmonella is one of the principal causes of foodborne outbreaks. As traditional control methods have shown less efficacy against emerging Salmonella serotypes or antimicrobial-resistant Salmonella, new approaches have been attempted. The use of lytic phages for the biocontrol of Salmonella in the food industry has become an attractive method owing to the many advantages offered by the use of phages as biocontrol agents. Phages are natural alternatives to traditional antimicrobial agents; they have proven effective in the control of bacterial pathogens in the food industry, which has led to the development of different phage products. The treatment with specific phages in the food industry can prevent the decay of products and the spread of bacterial diseases, and ultimately promotes safe environments for animal and plant food production, processing, and handling. After an extensive investigation of the current literature, this review focuses predominantly on the efficacy of phages for the successful control of Salmonella spp. in foods. This review also addresses the current knowledge on the pathogenic characteristics of Salmonella, the prevalence of emerging Salmonella outbreaks, the isolation and characterization of Salmonella-specific phages, the effectiveness of Salmonella-specific phages as biocontrol agents, and the prospective use of Salmonella-specific phages in the food industry.

• 한국멀티미디어학회논문지
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• 제22권12호
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• pp.1385-1395
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• 2019

In this paper, we propose a method to fabricate a patient-specific breast implant using MRI images and 3D scan data. Existing breast implants for breast reconstruction surgery are primarily fabricated products for shaping, and among the limited types of implants, products similar to the patient's breast have been used. In fact, the larger the difference between the shape of the breast and the implant, the more frequent the postoperative side effects and the lower the satisfaction. Previous researches on the fabrication of patient-specific breast implants have used limited information based on only MRI images or on only 3D scan data. In this paper, we propose an algorithm for the fabrication of patient-specific breast implants that combines MRI images with 3D scan data, considering anatomical suitability for external shape, volume, and pectoral muscle. Experimental results show that we can produce precise breast implants using the proposed algorithm.

• Food Science and Biotechnology
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• 제15권4호
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• pp.625-630
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• 2006

Polymerase chain reaction (PCR) method was developed for the specific detection of insect-resistant rice containing cry1Ac gene derived from Bacillus thuringiensis (Bt). Primers were designed from the 35S promoter, NOS terminator, cry1Ac gene, and sucrose phosphate synthase (SPS) for general screening of Bt rice. By sequencing the PCR products from the two putative kinds of Bt rice, we designed a specific primer from the junction region between the cry1Ac gene and the NOS terminator that had been inserted into Bt rice. The construct-specific primer was employed to amplify a 147 bp product in the two lines of Bt rice. No amplified products were observed from the other Bt crops with various Bt genes introduced. In qualitative PCR analysis, the limit of detection was 0.005 ng from genomic DNA of Bt rice. In addition, PCR analysis was performed on 64 kinds of rice presently available in the Korean market, and no Bt rice was detected. This method presented in this paper can be used as a highly sensitive and specific detection method of Bt rice.

• Journal of the Korean Wood Science and Technology
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• 제30권3호
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• pp.18-25
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• 2002

Research to discuss the fire performance of materials requires tools for measuring their burning characteristics and validated fire growth models to predict fire behavior of the materials under specific tire scenarios using the measured properties as input for the models. In this study, burning characteristics such as time to ignition, weight loss rate, flame spread, heat release rate, total heat evolved, and effective heat of combustion for four types of wood-based materials were evaluated using the cone calorimeter and inclined panel tests. Time to ignition was affected by not only surface condition and specific gravity of the tested materials but also the type and magnitude of heat source. Results of weight loss rate, measured by inclined panel tests, indicated that heat transfer from the contacted flame used as the heat source into the inner part of the specimen was inversely proportional to specific gravity of material. Flame spread was closely related with ignition time at the near part of burning zone. Under constant and severe external heat flux, there was little difference in weight loss rate and total heat evolved between four types of wood-based panels. More applied heat flux caused by longer ignition time induced a higher first peak value of heat release rate. Burning characteristics data measured in this study can be used effectively as input for fire growth models to predict the fire behavior of materials under specific fire scenarios.

• 한국축산식품학회지
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• 제22권2호
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• pp.183-191
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• 2002

몽골의 유가공 기술은 중앙아시아 지역의 유목민 생활방식의 독특한 전통에서 온 것으로 고유의 특성을 갖고 있다. 몽골의 전통적인 유가공 방식은 몽골의 토착종인 몽골우의 원유의 품질과 각 유성분 (단백질, 지방, 그리고 유당)의 허실 없는 완전이용 개념에 바탕을 두고 있다. 몽골에서는 다양한 종류의 유즙생산 동물로부터 젖을 취하여 가공하는 것이 발달했으므로 많은 종류의 유제품이 제조되고 있다. 몽골에서는 30종 이상의 유제품이 제조되고 있는데 그 중 36%는 발효유제품이 차지한다. 몽골의 전통적 발효유제품은 그 자체가 매우 특색이 있다. 왜냐하면 유산구균, 유산간균 그리고 효모가 함께 발효 유제품의 스타터로 사용되고 또한 함께 생육하고 있기 때문이다. 몽골에서는 유목민 생활의 여건 하에서 발효유제품에 쓰이는 유산균 스타터의 제조와 보관 방법이 매우 독특하게 전해져 오고 알다. 기존의 발효은 제품을 처리하여 스타터로 준비하거나 몇몇 특별한 야생식물로부터 유산균을 얻는 방식이 독특하다. 지금은 Rheum undulantum L., Rheum ribes L., Rumex acetosa L., Artemisa sibersena L., Artemisa vulgare와 같은 다섯 종류 이상의 야생식물이 유산균 스타터의 균원으로 사용되고 있다. 몽골에서는 젖을 가공할 때 소규모로서 매우 간단한 도구를 사용하는데 특히 발효유제품 가공기술은 배우기가 쉽고 나름대로의 과학적인 현상에 그 바탕을 두고 있다.

• 한국축산식품학회지
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• 제30권4호
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• pp.590-596
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• 2010

Meat and meat products are a potential source of food-borne pathogens, including Staphylococcus aureus, Salmonella spp., Escherichia coli O157:H7, and Bacillus cereus. A sensitive and specific PCR assay for the detection of these pathogens in meat and meat products was developed in this study, as part of a broader effort to reduce the potential health hazards posed by these pathogens. Initially, PCR conditions were standardized with purified DNA. Under standard conditions, the detection level for PCR was as low as 10 pg of purified bacterial DNA. After overnight growth of bacteria in a broth medium, as few as $10^2$ CFU of bacteria were detected by PCR assay. The primers employed in the PCR assay were found to be highly specific for individual organisms, and evidenced no cross-reactivity with heterologous organisms. Additionally, the multiplex PCR assays also amplified some target genes from the four pathogens, and multiplex amplification was obtained from as little as 10 pg of DNA, thus illustrating the excellent specificity and high sensitivity of the assay. In conclusion, this PCR-based technique provides a sensitive and specific method for the detection of S. aureus, Salmonella spp., E. coli O157:H7, and B. cereus in meat and meat products.

• Asian-Australasian Journal of Animal Sciences
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• 제32권6호
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• pp.891-895
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• 2019

Objective: Aim of present study was the set up of a fast and reliable protocol using species-specific markers for the quali-quantitative analysis of DNA and the detection of ruminant biological components in dairy products. For this purpose, the promoter of the gene coding for the ${\alpha}$-lactoalbumin (LALBA) was chosen as possible candidate for the presence of short interspersed nuclear elements (SINEs). Methods: DNA was isolated from somatic cells of 120 individual milk samples of cattle (30), Mediterranean river buffalo (30), goat (30), and sheep (30) and the gene promoter region (about 600/700 bp) of LALBA (from about 600 bp upstream of exon 1) has been sequenced. For the development of a single polymerase chain reaction (PCR) protocol that allows the simultaneous identification of DNA from the four species of ruminants, the following internal primers pair were used: 5'-CACTGATCTTAAAGCTCAGGTT-3' (forward) and 5'-TCAGA GTAGGCCACAGAAG-3' (reverse). Results: Sequencing results of LALBA gene promoter region confirmed the presence of SINEs as monomorphic "within" and variable in size "among" the selected species. Amplicon lengths were 582 bp in cattle, 592 bp in buffalo, 655 in goat and 729 bp in sheep. PCR specificity was demonstrated by the detection of trace amounts of species-specific DNA from mixed sources ($0.25ng/{\mu}L$). Conclusion: We developed a rapid PCR protocol for the quali-quantitative analysis of DNA and the traceability of dairy products using a species-specific marker with only one pair of primers. Our results validate the proposed technique as a suitable tool for a simple and inexpensive (economic) detection of animal origin components in foodstuffs.

• Human Ecology Research
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• 제56권2호
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• pp.167-174
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• 2018

This study investigated consumer innovativeness by examining a relationship model of innate innovativeness, product interest, product-specific innovativenss, and actualized innovativeness for eight product items as well as observed gender differences in the variables. The eight product items for the survey were smartphones, movies, clothing, cars, skin care products/cosmetics, traveling, AI products, and books. An online survey was conducted in January 2018, and 400 responses from male and female consumers in their twenties and thirties, were analyzed using reliability test, descriptive statistics, regression, and ANCOVA. The positive effect of innate innovativeness and product interest on product-specific innovativeness, and positive effect of innate innovativeness and product-specific innovativeness on actualized innovativeness were confirmed. Product interest was found to have a stronger power on product-specific innovativeness than innate innovativeness. Gender differences in product interest, product-specific innovativeness, and actualized innovativeness were identified as hypothesized. Female consumer's clothing interest, clothing innovativenss, and actualized clothing innovativeness were higher than male consumers; in addition, male consumer's cars and AI interest, cars and AI innovativenss, and actualized cars and AI innovativeness were higher than female's. The controversial results that indicated the higher innate innovativeness of male consumers useful to further in-depth discussion and research. This study contributed to the theory construction of consumer innovativeness research areas and offered practical implications for new product launching and customer relationship management.

• Asian Journal for Public Opinion Research
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• 제9권4호
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• pp.352-367
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• 2021

Purpose: This research aims to examine gender preferences for men and women advertising models in Saudi advertisements. Saudi Arabia is known as one of the most gender-segregated society in the world, and it has gender-specific roles, characteristics, and behaviors that are undesirable for the other gender. Methodology: The questionnaire was developed with the help of earlier studies on perceptions towards advertising models and validated by a jury of experts and focus groups. The gender preferences for ten product categories (including automobiles, baby care products, cigarettes, cosmetics for women, fashion, food & beverages, motorcycles, personal care for men, personal care for women, sporting goods) were examined for men and women models. Similarly, three personal preferences characteristics for both genders (face beauty, voice quality, and Islamic dress), two characteristics for women models (body shape, femininity), and two characteristics for men models (height-weight balance, masculinity) were examined for men and women models separately. Finally, a survey was conducted to solicit responses from respondents (N=412). Findings: Results indicated significant gender preferences for gender-specific product categories and typical gender stereotypes in advertising models. Men models were preferred in men-specific products, and women models were required in women-specific products. Some product categories (including personal care for men and sporting goods) were ranked higher for men advertising models, while for women advertising models, other product categories (including personal care for women and cosmetics for women) were ranked higher. Masculinity was ranked highest as the preferred personal characteristic for men advertising models, while voice quality was highest for women advertising models. Finally, there is a significant difference between the preferred personal characteristic for men and women advertising models for three characteristics, including face beauty, Islamic dress, and masculinity and femininity. Implications: Saudi Arabia is a unique society with predominantly unique cultural dominance. Consequently, local culture greatly influences advertisements. It has stereotyped gender roles even in advertisements. This study will establish a baseline for further research on the subject area.

• 대한안전경영과학회:학술대회논문집
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• 대한안전경영과학회 2004년도 추계학술대회
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• pp.189-195
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• 2004

It compared quality characteristics for the stone surface treatment system and its products. With the result, the flame burner equipment was mainly used to the surface treatment of granite, but it occurred greatly the high temperature, the rock fragment, the noise and dust. For the other side, the whetstone polishing machine for the polishing was a maintenance for the specific physical properties of stone, and it did not occur the stone fragment and the dust. The durability of the stone products due to the flame burner was investigated with that it falls to $20\sim25\%$ more than the surface treatment by the whetstone polishing. Share's hardness of the polishing products in the durability test showed more greatly index than the flame burner and conventional product. The polishing products of Pochon stone in the case of the abrasion resistance showed great more $15\%$ than the general products, more $9\%$ than the products by the flame burner.