• Journal of Microbiology and Biotechnology
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• 제15권6호
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• pp.1377-1383
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• 2005

In a cDNA microarray experiment using Cy3 and Cy5 as labeling agents, particularly for the direct design, cDNAs from some genes incorporate one dye more efficiently than the other, which is referred to as the gene-specific dye bias. Dye-swaps, in which two dyes are switched on replicate arrays, are commonly used to control the gene-specific dye bias. We developed a simple procedure to extract the gene-specific dye bias information from a partial dye swap experiment. We detected gene-specific dye bias by identifying outliers in an X-Y plane, where the X axis represents the average log-ratio from two sets of dye swap pairs and the Y axis exhibits the average log ratio of four forward labeled arrays. We used this information for detecting differentially expressed genes, of which the additionally detected genes were validated by real-time RT-PCR.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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• pp.24-24
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• 2003

Fluorescence in situ Hybridization(FISH)는 특정 염기서열을 이용하여 염색체나 염색체상의 DNA위치를 확인하는 기술로서, 면역세포화학 기술과 결합되어져 현미경으로 이들의 유전적 활성도를 직접 확인할 수 있는 방법으로 지금까지의 radioisotopes 대신 non-radioactive labeling 방법으로서 fluorescence을 이용한 분자세포유전학적 검정 방법이다. 따라서 특정 염색체의 FISH probe의 개발은 FISH 기법을 이용하여 조직 또는 세포내 특정 염색체나 DNA의 존재나 이상 유무를 신속하고 정확하게 파악할 수 있다. 본 연구는 소와 사람을 대상으로 Y-염색체 특이 DNA probe를 개발하고 이를 이용하여 FISH를 시행함으로서 본 probe의 신뢰성을 확인하고 임상적 적용 가능성을 제시 하고자 하였다.

• 미생물학회지
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• 제26권1호
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• pp.27-31
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• 1988

Biosynthesis and processing of cytoplasmic mRNA from heterogenous nuclear RNA (hn-RNA) in Aspergillus phoenicis were studied by $^{3}H$-uridine labeling and synchronous culture techniques during their life cycle. Incorporations of $^{3}H$-uridine into hn-RNA and mRNA were most rapid in vesicle-phialide fromation stage and diminished in hyphal growth stage. The processing of cytoplasmic mRNA from hn-RNA was proceeded more rapidly in hyphal growth and conidiophore formation stages than in conidia and vesicle-phialide formation stages. The specific radioactivities of hn-RNA and mRNA were very high in vesicle-phialide formation stage.

• Korean Chemical Engineering Research
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• 제60권3호
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• pp.398-406
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• 2022

그람음성균의 외막은 수많은 생물물리학적 및 생화학적 과정이 작용하여 생존력을 유지하도록 설계되어 있는 세포환경의 가장 바깥 층이다. 세포공학의 발전으로 인해 박테리아의 막 환경을 변경하는 등 유전정보를 원하는 대로 조작할 수 있게 되었고 이는 박테리아를 특정 목적에 적용시킬 수 있게 하였다. 그중 기능성 분자를 박테리아 외막에 표지하는 세포 표면공학은 숙주세포가 특정 외부물질이나 자극에 반응하도록 유도하는 전략 중 하나이다. 기능성 펩타이드 또는 단백질을 세포 표면에 표지하기 위한 방법으로 막 고정 모티프를 융합한 후 세포 내에서 발현하는 방법이 일반적으로 사용되고 있지만 이는 박테리아 시스템에서 발현할 수 없는 외인성 단백질이나 크기가 큰 단백질에는 적용할 수 없다는 한계점이 있다. 박테리아 외막의 구성요소에 자연적으로 존재하는 반응성 그룹과 기능성 물질을 화학접합하는 방법도 있으나 필수 구성 요소의 비특이적 변형으로 인해 세포의 생장이 저해되는 경우가 많다. 본 연구에서는 비천연아미노산 또는 자가결합 도메인을 사용해 대장균의 세포 표면을 부위 특이적으로 형광 표지하는 두 가지의 접근법을 수행하였다. 첫 번째 접근법은 화학선택적 반응성을 지닌 비천연아미노산이 삽입된 펩타이드를 대장균 표면에 발현하여 위치 특이적으로 형광염료를 접합시키는 방법이다. 두 번째 접근법은 자가결합능력을 지닌 이종 이량체 코일-코일에서 유래된 α-나선 도메인을 대장균 외막에 발현하고 녹색 형광 단백질이 융합된 상보적인 α-나선 도메인을 막 표면에 특이적으로 고정하는 방법이다. 제시된 방법들은 위치와 시간이 제어된 방식으로 박테리아 외막에 새로운 기능을 부여하는 방법론으로서 유용하다.

• 가정과삶의질연구
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• 제20권4호
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• pp.89-102
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• 2002

Genetically modified foods may be defined as the foods deemed as safe by current technology among the many kinds of agricultural and stockbreeding products that are now under research and development using contemporary gene-modification techniques. This study examines hotly debated arguments, both for and against genetically modified- foods, in various countries. This study also investigates consumers'rights and responsibilities. Countries that are developing and exporting genetically modified organisms(GMO) have maintained that GMO can help produce more crops while reducing labor and other production-related costs, and that the genetically modified foods signify ″the second green revolution,″ which will solve future food and environmental problems by strengthening specific nutritive substances and extending shelf-life. But consumer groups, environmental organizations. and food-importing countries are more cautious about importing and consuming those foods because the potential dangers of GMO to human bodies and the environment have not been tested thoroughly yet. South Korea, following suit with others such as EU, Japan, Australia, and New Zealand, introduced a law on 'Labeling of Genetically Modified Foods', which went in effect in March, 2001, on the basis of customers'rights to make informed choices. The law takes the ″precautionary principle″ into consideration, rather than stopping at insuring ″substantial equivalence″ in developing and consuming GM foods. The actual impact of the law will depend on the level of citizens'Participation more than on the government's willingness to carry out the law. So far the level of Korean consumers'consciousness about genetically modified foods is very low. Therefore, it is hard to expect consumers to exercise their ″rights not to buy″ foods that are potentially unsafe. The Korean government must devise an effective plan to inform and educate the people about the labeling of genetically modified foods.

• 정보과학회 논문지
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• 제43권7호
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• pp.773-780
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• 2016

기계학습 기반의 의미역 인식에서 어휘, 구문 정보가 자질로 주로 쓰이지만, 의미 정보를 분석하는 의미역 인식은 의미 정보 또한 매우 유용한 정보이다. 그러나, 기존 연구에서는 의미 정보를 활용할 수 있는 방법이 제한되어 있기 때문에, 소수의 연구만 진행되었다. 본 논문에서는 의미 정보를 활용하는 방안으로 동형이의어 수준의 의미 애매성 해소 기술, 고유 명사에 대한 개체명 인식 기술, 의미 정보에 기반한 필터링, 유의어 사전을 이용한 클러스터 및 기존 의미 프레임 정보 확장, 구문-의미 정보 연동 규칙, 필수 의미역 오류 보정 등을 제안한다. 제안하는 방법은 기존 연구 대비 뉴스 도메인인 Korean Propbank는 3.77, 위키피디아 문서 기반의 Exobrain GS 3.0 평가셋에서는 8.05의 성능 향상을 보였다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2001년도 추계학술대회 논문집 전기물성,응용부문
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• pp.274-276
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• 2001

This research aims to develop the multiple channel electrochemical DNA chip using microfabrication technology. At first, we fabricated a high integration type DNA chip array by lithography technology. Several probe DNAs consisting of thiol group at their 5-end were immobilized on the sold electrodes. Then target DNAs were hybridized and reacted. Cyclic voltammetry showed a difference between target DNA and control DNA in the anodic peak current values. Therefore, it is able to detect a plural genes electrochemically after immobilization of a plural probe DNA and hybridization of non-labeling target DNA on the electrodes simultaneously. It suggested that this DNA chip could recognize the sequence specific genes.

• 대한방사성의약품학회지
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• 제3권1호
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• pp.3-14
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• 2017

Nucleophilic aromatic fluorination has been one of the most explored methods in fluorin-18 based radiochemistry. Unlike electrophilic $[^{18}F]$fluorination methods, no-carrier-added nucleophilic radiofluorination with cyclotron-produced $[^{18}F]$fluoride ion offers better specific radioactivity which is essential aspect to obtain good quality images from positron emission tomography. Contrary to amenable aliphatic radiofluorination, the development of reliable aromatic $[^{18}F]$fluorination methods has been pursued by many research groups; however, no viable method has yet been established. Recently, hypervalent iodine compound draws increasing attention as versatile radiolabeling precursor for various $[^{18}F]$fluoroarenes, since it bears the capacity to introduce fluorine-18 either on electron-deficient or electron-rich aryl ring with enhanced regiospecificity. Other classes of hypervalent iodine congeners often utilized in radiochemistry are iodylarenes, iodonium ylides, and spirocyclic iodonium ylides. Recently developed spirocyclic iodonium ylides have already been avidly employed to provide various $[^{18}F]$aryl fluorides with high labeling efficiency. This metal-free protocol would afford efficient routes, replacing the traditional approaches to $[^{18}F]$fluoroarenes, from prosthetic labeling synthons to complex PET radiotracers.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.75.2-75
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• 2003

To develop an effective protection strategy against tomato bushy stunt virus (TBSV), tobacco plants expressing single-chain Fv antibodies (scFv), were established. A previous had shown that the replication activity of viral replicase was inhibited by the selected scFvs. Moreover, no systemic symptom was found after virus inoculation on leaves of wt N. benthamiana infiltrated with an Agrobacterium suspension resulting i3l expression of the scFvs. However, control plants showed systemic symptoms. In this study the localization of the scFvs within two transgenic plant lines, (CP28H3, CP-P55) was demonstrated using immunogold labelling. The gold particles, indicating the presence of scFv, were mostly found In the cytoplasm of the plant cells including chloroplasts and in the cell walls. However, they were hardly found in the vacuole, nucleoplasm and intercellular spaces. Gold particles often accumulated in either the cytosol or chloroplasts showing a specific labeling, There was no difference in type of gold labeling between both transgenic lines. The localization of the scFv in the cytoplasm further conforms the inhibition of the RNA-dependent RNA polymerase (RdRp) by the selected scFv because it is known that the RdRp is localized to membraneous cytosolic structures.

• Toxicological Research
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• 제17권
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• pp.185-188
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• 2001

Genetically modified foods and food additives are derived from organisms that have been inserted foreign genetic materials by recombinant DNA techniques to improve the quality or any other pur-poses. The problems such as toxicity, allergenicity and antibiotics resistance in the safety of genetically modified foods are usually concerned. In Korea, the safety of foods is ensured by the Food Sanitation Act. Although there is no specific provision regarding the genetically modified foods in it, any foods that might cause negative effect(s) on public health or human life are prohibited to sell in the market. In order to systematically evaluate safety of genetically modified foods, the Korea Food and Drug Administration (KFDA) promulgated "Guidelines regarding review of safety assessment data for genetically modified foods and food additives (KFDA Notification 1999-46)". The objectives of these guidelines are to ensure safety of genetically modified foods and food additives. In order to evaluate the safety of genetically modified foods. KFDA operates a special expert committee composed by experts from government, universities, research institutes. and consumer's unions. Recently. manufacturers and consumers are interested in the issues on safety and labeling of genetically modified foods, because of increment of imported genetically modified crops and processed foods. Since government and consumers unions have different viewpoints, their positions regarding the issue are different each other. Therefore, the regulation of labeling on genetically modified foods is prepared and should be enforced at July 2000 in Korea. in Korea.