• Clinical and Experimental Pediatrics
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• 제50권5호
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• pp.416-421
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• 2007

Many previous studies have proved that human allergic disease resulted from the formation of antibodies belonging to a unique immunoglobulin isotype termed immunoglobulin E (IgE). Most of IgE-producing plasma cells are found in the lymphoid tissue associated with the gastrointestinal and respiratory tracts. IgE may be found free in the mucosal secretions of these tissues, bound to local mast cells, or distributed by the systemic circulation to mast cells and basophils throughout the body. Total serum IgE concentrations tend to be higher in allergic adults and children compared with non-allergic individuals, but the value of total serum IgE as a screening test for allergic disease is limited. Total serum IgE levels are related to the probability of an individual having detectable allergen-specific IgE. Allergen-specific IgE concentrations vary with a person's age, the degree and duration of the recent allergen or cross-reactive allergen exposure. The value of quantitative assays for allergen-specific IgE has been suggested in recent studies. Serum IgE increases in many non-allergic diseases, including infectious and parasitic diseases. The IgE changes appear to be specific to the infectious agents, whereas non-specific in other diseases. The increased serum IgE in some of these conditions probably results from alterations in immune function. This review summarizes the clinical significance of total and allergen-specific IgE examinations in allergic diseases.

• Nutrition Research and Practice
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• 제6권5호
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• pp.429-435
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• 2012

Atopic dermatitis (AD) has become a serious epidemic in Korean children. We aimed to investigate the association between vitamin C, E and other nutrients, and serum total IgE/specific IgE levels in children with AD. A total of 119 children (0-24 mo) diagnosed with AD were recruited for this cross-sectional study from a medical center in Seoul. A 24 h recall was used to assess dietary intakes. Serum total and six food-allergen specific IgE levels were measured by CAP-FEIA. Serum vitamin E was also measured but only in 25 out of the total 119 participants. Multiple linear regression analysis was performed to estimate the coefficients between serum IgE levels and dietary intake as well as serum vitamin E. Serum vitamin E levels showed a significantly inverse association with serum total IgE and all specific IgE levels (P < 0.05). Fat intake was inversely related with specific-IgEs for egg whites, milk, buck wheat, soy, and peanuts (P < 0.05). Positive associations were found between carbohydrate (CHO) intake and total IgE and specific IgEs to egg whites, milk, soy, and peanuts (P < 0.05). Vitamin C, E and n-3/n-6 fatty acids were not related with serum total IgE and specific IgE levels except for the association between buck wheat and vitamin E. In addition, there were no significant differences between males and females in dietary intake and serum IgE levels by student's t-test. Although dietary vitamin E showed no association with serum IgE levels, serum vitamin E drew a significant inverse relationship with serum IgE levels. The evidence seems to suggest that vitamin E may possibly lower total and specific-IgEs in children with AD, and that it is important to maintain a relatively high serum vitamin E level in children with AD.

• Toxicological Research
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• 제17권
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• pp.197-199
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• 2001

Enhancement of antigen-specific IgE is a hallmark of allergic hyperresponsiveness, therefore it is necessary to adopt or develop a highly sensitive and specific assay for determination of allergen-specific IgE levels in vivo. In this presentation, we introduce an ELISA (enzyme linked immunosorbent assay) system developed to measure the levels of chicken egg ovalbumin (OVA)-specific IgE in serum. The ELISA method uses a commercially available purified rat anti-mouse IgE as a capture Ab and biotinylated OVA as a detection reagent. Avidin-peroxidase with its substrate is used for color development resulting in optical density measurement at 405 nm. The ELISA system produces a highly sensitive dose-response relation-ship between optical density levels and the dilution titer of the OVA-IgE standard serum but no cross-reaction with unrelated IgE or IgG. It is believed that the system is an Efficient tool to delineate an adjuvant effect of environmental pollutants on development of asthmatic and atopic responses.

• Clinical and Experimental Pediatrics
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• 제49권10호
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• pp.1061-1066
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• 2006

목 적 : 신생아에서 우유 알레르기가 의심되는 경우 우유 특이 IgE 항체 측정이 우유 알레르기 진단에 유용한지 알아보고자 하였다. 또한 추적관찰이 가능한 경우 우유 특이 IgE 항체 검사결과와 다른 알레르기 질환의 발현과의 연관성도 알아보고자 하였다. 방 법 : 생후 4주이내의 신생아로 우유 특이 IgE 항체 검사양성을 보인 87명을 대상으로 하였다. 대상 환아는 병력 청취와 진찰 소견에서 우유 알레르기가 의심되어 우유 단백 제거 요법을 시행하여 증세의 호전을 보이고 우유 특이 IgE 항체 검사에서 양성을 보인 환아로 하였다. 또한 이들 87명의 환아 중 추적관찰이 가능했던 40명을 대상으로 우유 특이 IgE 항체 검사결과와 알레르기 질환 발현과의 상관관계를 알아보았고 이들 항체와 알레르기 질환의 가족력과의 상관관계도 알아보았다. 결 과 : 대상 환아의 평균 나이는 $17.2{\pm}5.4$일 이었다. 우유 특이 IgE 항체 검사 결과 milk 양성은 87명이었고 casein 양성은 24명, ${\alpha}$-lactoalbuminn 양성은 38명, ${\beta}$-lactoglobulin 양성은 75명이었다. 6개월 이상의 추적관찰이 가능했던 환아는 40명(45.9%)이었고 부모가 알레르기 질환을 가진 경우는 10명(25%), 환아의 추적 관찰 결과 알레르기 질환을 가진 경우가 15명(37.5%)으로 천식 4명, 아토피 피부염 11명이었다. 추적관찰 결과 신생아기에 casein에 양성을 보인 경우 알레르기 질환의 발현과 연관성이 통계적으로 유의하게 높았다. 그러나 우유 특이 IgE 항체 양성과 알레르기 가족력과의 연관성은 통계적 유의성이 없었다. 결 론 : 신생아에서 IgE 매개성 및 혼합성 우유 알레르기가 의심되는 경우는 우유 제거 식이 요법과 우유 특이 IgE 항체 검사를 시행한다면 진단에 도움이 될 수 있다고 생각된다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
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• pp.244-244
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• 1994

Cortex Mori, the root bark of mulbery tree has been used as an antiphlogistic, diuretic, and expectorant in herbal medicine. The purpose of this study is to evaluate chicken gamma globulin (CGG)-specific IgE-induced morphologic and functional changes in rat peritoneal mast cells (RPMC), and to determine whether Cortex Mori could inhibit the CGG-specific IgE-depeildent mast cell degranulation and histamine release from RPMC. Results are 1) the degranuration and histamine release from RPMC were not induced within 1 hour after addition of Cortex Mori alone, 2) the CGG and CGG-specific IgE-Induced degranulation from RPMC was observed within 10 minutes, 3) the histamine release from RPMC sensitised with CGG-specific IgE was induced by tile addition of CGG, 4) CGG-specific IgE-dependent degranulation rate in RPMC pretreated with Cortex Mori was significantly Inhibited, compared to that of control group without Cortex Mori pretreatment, and 5) the CGG-specific IgE-dependent histamine release from RPMC was significantly inhibited by pretreatment with Cortex Mori. These data suggest that Cortex Mori contains some substances with capabilities to inhibit CGG-specific IgE-dependent degranulation and histamine release from RPMC.

• 한국콘텐츠학회논문지
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• 제9권4호
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• pp.236-246
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• 2009

알레르기 환자의 증가로 인하여 알러겐을 찾는 것은 중요하다. MAST는 알러겐 특이 IgE항체를 측정하는 간단한 방법이다. 본 연구에서는 알레르기 환자에서 알러겐 특이 IgE 항체, 총 IgE, 호산구수와의 관련성을 평가하고자 하였다. 연구 결과 총 IgE 양성률은 흡입형 패널에서 96.97%, 식품형 패널에서 98.06%였다. 알러겐 특이 IgE 항체의 양성률은 흡입형 패널에서 집먼지 51.52%, 북아메리카 집먼지 진드기 45.46%, 고양이 31.99% 였으며, 식품형 패널에서 각각 55.34%, 42.72%, 34.96%였다. 혈청 총 IgE는 알레르기와 관련이 있었으나, 알레르기가 항상 호산구증가와 관련이 있는 것은 아니었다.

• 생명과학회지
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• 제17권11호
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• pp.1492-1496
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• 2007

CD40L를 발현하는 KU812세포와 CD40를 발현하는 항원 특이적 B 세포의 배양을 통해 IgE 클라스 스위치가 유도된다. 본 연구자는 체외면역법에 의해 건강인의 말초혈액을 이용하여 쌀 알레르겐 특이적 IgM 항체를 생성하는 B 세포주를 수립하였다. 본 연구에서는 KU812세포에 의한 쌀 알레르겐 특이적 IgE 항체의 유도를 시도하였다. 배양 전, 쌀 알레르겐 특이적 B 세포주, RA9G11과 PMA와 ionomycin을 6시간 처리하여 활성화된 KU812세포에서 CD40와 CD40L 발현량을 flow cytomerty를 통화여 각각 확인하였다. RA9G11세포와 활성화된 KU812세포는 높은 수준의 CD40와 CD40L를 각각 발현하였다. 쌀 알레르겐 특이적 IgE항체 합성 유도를 위해, 여러 가지 농도의 IL-4 존재하에, RA9G11세포와 활성화된 KU812세포를 12일 동안 배양하였다. IgE 정상영역의 mRNA 발현량과, 쌀 알레르겐 특이적 IgE 항체 생성 세포가 모든 조건에서 확인되었으며, 특히 50U의 L-4 농도 하에서 같은 비율의 RA9G11과 활성화된 KU812세포를 배양했을 때, IgE 합성이 가장 높았다. 따라서 활성화된 KU812세포에 의한 쌀 알레르겐 특이적 IgE 합성은 알레르기 질환의 치료 및 예방에 관한 연구에 기여할 것으로 사료된다.

• Clinical and Experimental Pediatrics
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• 제58권3호
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• pp.89-95
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• 2015

Purpose: House dust mite (HDM) has been suggested to be the most important aeroallergen responsible for atopic asthma in Korea. We aimed to investigate that specific IgE antibodies to HDM and other common indoor aeroallergens contribute differently to total serum IgE and show different relationships with longitudinal fractional exhaled nitric oxide (FeNO) measurements in Korean atopic asthmatic patients. Methods: A total of 193 children aged 8 to 16 years with intermittent or mild persistent atopic asthma were recruited. Sera were assayed for total IgE and specific IgE antibodies to HDM and other common indoor allergens. FeNO was serially measured 10 times or more over 2 years when subjects were not receiving controller medications. Results: In 152 children who completed the study, IgE antibodies to specific HDM were more prevalent than those to other common indoor aeroallergens. In addition, IgE antibody titers to HDM were the strongest contributor to total IgE increases. Furthermore, only HDM-specific IgE antibody titer significantly correlated with maximum FeNO (r=0.21, P=0.029) and the rate of FeNO higher than 21 parts per billion (ppb) (r=0.30, P=0.002). Eight patients (5%) were found to have maximum FeNO of 21 ppb or less, suggesting the presence of a low FeNO phenotype among atopic asthmatic patients. Conclusion: The quantity of HDM-specific IgE antibody provides a possible explanation for increases of total IgE and significantly correlates with the amount and frequency of FeNO increases in Korean atopic asthmatic patients.

• 한국동물위생학회지
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• 제46권3호
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• pp.219-225
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• 2023

Canine atopic dermatitis (CAD) has a genetic basis and is primarily a skin disorder characterized by allergic features. It is caused by an excessive immune response, associated with immunoglobulin E(IgE), to allergens and shows clinical signs of itching and inflammation. The present study evaluated the allergen-specific and total IgE concentration in dogs with allergic disorders. Allergen-specific IgE analysis was performed using a Multiple Allergen Simultaneous Test (MAST) assay, and total IgE analysis was conducted using an ELISA in 82 dogs with CAD. The subjects were divided into two groups: dogs aged ≤3 years and dogs aged >3 years, enabling a comparison of total IgE concentrations between the two age groups. The results showed that the major affecting environmental allergen was Alternaria alternate, and the major affecting dietary allergen was raw chestnut. The results revealed that dogs aged ≤3 years had higher total IgE concentrations than dogs aged >3 years with significant difference. The results of this study can contribute to the diagnosis and treatment of CAD in South Korea.

• IMMUNE NETWORK
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• 제8권3호
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• pp.82-89
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• 2008

Background: Although a skin test is the primary option for detecting allergen-specific IgE in clinics, the serum IgE immunoassay is also important because it allows for the diagnosis of allergy without any accompanying adverse effect on the patient. However, the low detection limit of IgE levels by immunoassay may restrict the use of the method in some occasions, and improving its sensitivity would thus have a significant implication in allergy-immunology clinics. Methods: In this study, we attempted to detect specific serum IgE by using immuno-polymerase chain reaction (IPCR) which combines the antigen-antibody specificity of enzyme-linked immunosorbent assays (ELISAs) with the amplification power of PCR. Results: Our results demonstrated that Blo t5-specific serum IgE can be detected by IPCR with a 100-fold higher sensitivity than ELISA, and cross-reactivity of serum IgE to other mite allergens is able to be analyzed by using only $0.3{\mu}l$ of serum sample. Use of real-time IPCR seemed to permit more convenient determination of specific serum IgE as well. Conclusion: We believe that IPCR can serve as a valuable tool in determining specific serum IgE, especially when the amount of serum sample is limited.