• 한국산림과학회지
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• 제31권1호
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• pp.37-42
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• 1976

본시험(本試驗)은 천보산지역(天寶山地域)의 황폐지(荒廢地)에서 효과적(效果的)인 지피식생(地被植生) 조성방법(造成方法) 구명(究明)코자 실시(實施)되었다. 이 지역(地域)은 심한 면상침식(面狀浸蝕)이 일어난 곳으로서 모재(母材)는 화강편마암(花崗片麻岩)으로 매우 침식위험(浸蝕危險)이 높다. 참싸리, 새, 아까시나무가 공시초목(供試草木)으로 사용 되었고 삽종방법(揷種方法)은 산파(散播)거적피복(被覆)과 산폐무피복(散廢無被覆), 조파(條播) 10cm와 조파(條播) 20cm, 점파(點播) 20cm와 점파(點播) 30cm 이상(以上) 3파종(播種) 6처리(處理)였다. 1. $30^{\circ}$이하(以下)의 경사지(傾斜地)에서는 산파(散播)거적피복방법(被覆方法)이 가장 효과적(效果的)이었다. 2. $30^{\circ}$이상(以上)의 급경사지(急傾斜地)에서는 조파(條播) 10cm가 효과적(效果的)이었다. 3. 토성(土性)은 토양수분(土壤水分)을 조절(調節)하여 식생조성(植生造成)에 영향을 준 것 같다. 피복도(被覆度)와 생중량(生重量)은 토양중(土壤中)의 세토량(細土量)과 함께 증가(增加)하였다. 4. 해가 지남에 따라 피복도(被覆度)는 생중량(生重量)과 함께 증가(增加)하였으나 생립본수(生立本數)는 감소(減少)하였다.

• 약학회지
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• 제51권4호
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• pp.285-290
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• 2007

The oxidative stress causes many diseases like cancer, aging, cardiovascular disease, degenerative neurological disorders (Parkinson’s disease, and Alzheimer's disease) by damage of cell membrane, protein deformation, and damage of DNA due to the oxidation of lipid of cell membrane, protein of tissue or enzyme, carbohydrate, and DNA. It is caused by the reactive oxygen species (ROS) that is produced in the metabolic process of oxygen in cell. The superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in cell systemize the antioxidative enzymes to control the oxidative stress. In this research, it is measured that the survival rate of cell by the typical isoflavonoid of daidzein or genistein, activity of antioxidative enzyme, and ROS level, in order to study the effect of isoflavonoid over the ROS production in cell and antioxidative system. As the similar action of the isoflavonoid with the estrogen is examined, women are encouraged to get bean. In view of this trend, it is very important to find out a combination medicine that lowers the oxidative stress caused by the daidzein in the ovarian cell. In the combined treatment of the typical antioxidant of vitamin C to oxidative stress which induced by daidzein recover the control level particularly lowering the ROS in cell by 30%. However, it made no effect in the combined treatment with genistein. Therefore, the research took the combination effect of daidzein with vitamin C in order to check it effect over the antioxidative system. In conclusion, it was disclosed that the oxidative stress caused by daidzein is related to the lowering activity of SOD, and the specific combination effect of daidzein with vitamin C is related to the recovery of SOD activity.

• 한국응용곤충학회지
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• 제46권1호
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• pp.165-168
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• 2007

고구마 재배지에 발생하는 풍뎅이류 유충(굼벵이)의 종류 및 종별 피해양상을 조사하였다. 고구마 재배지 토양에 발생하는 풍뎅이류는 큰검정풍뎅이(Holotrichia parallela), 참검정풍뎅이(Holotrichia diomphalia), 애풍뎅이(Anomala rufocuprea) 3종이지만 주로 발생하는 풍뎅이류는 대부분 큰검정풍뎅이로 조사되었다. 굼벵이류에 의한 고구마 괴근 피해율은 지역에 따라 차이가 있었는데 평군 2-40%였고, 심한 경우는 80% 이상의 피해를 나타낸 포장도 있었다. 큰검정풍뎅이는 10월 하순부터 다음해 6월 하순까지 토양 속에서 3령의 노령유충으로 월동하였으며, 월동기간 동안 유충의 생존율은 92%로 매우 높았다. 고구마 포장에서 큰검정풍뎅이 유충은 7월 하순부터 8월 중순 사이에 발생하기 시작하였고, 고구마의 굼벵이 피해는 8월 하순부터 나타나기 시작하여 수확기까지 지속되었다.

• 한국임상약학회지
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• 제22권2호
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• pp.181-186
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• 2012

Objective: To report a fatal case of Multidrug-resistant Acinetobacter baumannii (MDR-AB) in a patient with interstitial lung disease (ILD) on high-dose glucocorticoids. Case Summary: A 66-year-old man with a history of coniosis was transferred to the hospital with progressive cough and sputum production. This patient has been diagnosed with pneumonia and ILD on admission, requires antimicrobial therapy and systemic immunosuppressants. He received high dose of methylprednisolone and cyclophosphamide for ILD as well as ceftriaxone and azithromycin for pneumonia. On day 7 in the intensive care units (ICUs), patient had fever and leukocytosis, thus antimicrobials were switched to piperacillin. After 13 days in the ICU, Acinetobacter baumannii and methicillin-resistant Staphylococcus aureus (MRSA) were isolated on transtracheal aspirate (TTA) and meropenem was initiated. However, it was revealed a multidrug-resistant Acinetobacter baumannii (MDR-AB) species, resistant to carbapenem. Patient was administered colistin but expired due to septic shock on day 84. Discussion: Systemic immunosuppressive therapy can result in infections that may compromise patient's survival. MDR-AB has emerged as a serious cause of nosocomial infections in immunocompromised patients. MDR-AB is resistant to most standard antimicrobials and therapeutic options are limited. Conclusion: We report our recent experience with a fatal MDR-AB pneumonia in a patient with ILD, who had to be treated with high dose glucocorticoids and immunosuppressnts.

• 콘크리트학회논문집
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• 제16권5호
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• pp.591-596
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• 2004

제강슬래그 골재의 소파블록 적용성을 평가하기 위하여, 구조적 재료로서의 콘크리트 물성을 연구하였다. 또한, 제강슬래그 골재를 사용한 해양 콘크리트의 생물학적 연구를 수행하였다. 수행된 콘크리트의 물성 실험은 슬럼프, 공기량, 압축강도, 쪼갬인장강도, 탄성계수, 탄산화 저항성, 수화열, 동결융해, 황산염 침지, 건조수축 실험 등이다. 또한, 해양 저서생물과 해조류의 번식 및 증식에 대한 조사를 위해 생물학적 실험이 수행되었다. 본 연구결과에 따르면, 제강슬래그 골재의 함유는 압축강도, 쪼갬인장강도, 그리고 탄성계수에 대하여 나쁜 영향을 미치지 않으며, 콘크리트의 내구성 측면에서도 제강슬래그 골재의 함유에 의한 악영향을 받지 않았다. 생물학적 연구에 따르면, 제강슬래그 골재는 해양 저서생물과 해조류의 증식과 번식에 이상적인 재료로서 평가되었다.

• 한국발생생물학회지:발생과생식
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• 제17권3호
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• pp.231-240
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• 2013

Olive flounder (Paralichthys olivaceus) is one of the commercial important flatfish species in Korea. The ocular signal transduction pathway is important in newly hatched flounders because it is closely involved in the initial feeding phase thus essential for survival during the juvenile period. However, the study of gene expression during ocular development is incomplete in olive flounder. Therefore we examined the expression analysis of specifically induced genes during the development of the visual system in newly hatched flounders. We searched ocular development-involved gene in the database of expressed sequence tags (ESTs) from olive flounder eye and this gene similar to arrestin with a partial sequence homology. Microscopic observation of retinal formation corresponded with the time of expression of the arrestin gene in the developmental stage. These results suggest that arrestin plays a vital role in the visual signal transduction pathway of the retina during ocular development. The expression of arrestin was strong in the ocular system during the entirety of the development stages. Our findings regarding arrestin have important implications with respect to its biological role and evolution of G-protein coupled receptor (GPCR) signaling in olive flounder. Further studies are required on the GPCR-mediated signaling pathway and to decipher the functional role of arrestin.

• 한국수정란이식학회지
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• 제26권3호
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• pp.171-180
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• 2011

In this study I report that in vitro development rates of bovine nuclear transfer embryos activated either with boar sperm cytosolic factor (SCF) or with ionomycin followed by cycloheximide (CHX) and subsequent in vivo developmental rates after embryo transfer are related to blastocyst quality as evaluated by apoptosis analysis. SCF was extracted from porcine semen then purified for post-activation injection after nuclear transfer. The optimal timing for SCF injection was determined to be at least 22 h post-IVM for parthenogenetic activation of bovine oocyies. A total of 364 oocytes were successfully enucleated and 268 (73.6%) fused and were injected with SCF. The survival rate of fused and injected embryos was 109/113 (96.5%) after 2 h. The cleavage rates of nuclear transfer embryos after 3 d of culture in the ionomycin/CHX treated group were significantly higher than those of the SCF-activated group (93.3% vs 81.7%, p<0.01, respectively). However, at 7 d and 9 d there was no significant difference between the total developmental rates to blastocyst for either treatment group. Total blastocyst cell numbers were also not significantly different between the two activation treatments (ionomycin/CHX: 149.5${\pm}$7.7 vs. SCF: 139.3${\pm}$4.4 cells). In contrast, the apoptotic levels in the SCF blastocysts were higher than those produced after the chemical treatment (28.2${\pm}$5.1% vs. 8.8${\pm}$0.6%, respectively). A total of 18 expanded or hatching blastocysts was transferred to nine synchronized recipients in each activation group; 5/9 (55.5%) and 2/9 (22.2%) were pregnant at 40 d in the ionomycin/CHX treatment and SCF activated group, respectively. However, only one went to term in the ionomycin/CHX treatment while none of the pregnancies from the SCF group were maintained by 90 d. In conclusion, these results suggest that SCF derived from different species is a limited activator to be used for activation after bovine nuclear transfer in lieu of a chemical activation protocol.

• 대한미생물학회지
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• 제34권5호
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• pp.435-443
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• 1999

Orientia tsutsugamushi is obligate intracellular bacterium that grows within the cytoplasm of the eukaryotic host cells. Therefore capability of the attachment, entry into the host cell and intracellular survival should be critical process for oriential infection. In this study we investigated the cellular invasion mechanism of Orientia tsutsugamushi and the role of transmembrane heparan sulfate proteoglycan, which binds diverse components at the cellular microenvironment and is implicated as host cell receptors for a variety of microbial pathogens. First of all Orientia tsutsugamushi can invade a wide range of nonprofessional phagocytic cells including fibroblast, epithelial cells and endothelial cells of various host species, including Band T lymphocytes. Thus, it was postulated that the attachment of O. tsutsugamushi requires the recognition of ubiquitous surface structures of many kinds of host cells. Treatments with heparan sulfate and heparin inhibited the infection of Orientia tsutsugamushi in dose-dependent manner for L cell, mouse fibroblast, whereas other glycosaminoglycans such as chondroitin sulfate had no effect. Collectively, these findings provide strong evidence that initial interaction with heparan sulfate proteoglycan is required for the oriential invasion into host cells.

• 생약학회지
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• 제45권4호
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• pp.275-281
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• 2014

After harvesting the medicinal parts of Curcuma longa, the remaining underground parts were discarded. From the remaining underground parts of Curcuma longa quercetin-3-O-${\beta}$-D-glucopyranoside-7-O-${\alpha}$-L-rhamnopyranoside (Q37) was isolated. The antioxidant activities in vitro and lifespan-extension effect of Q37 were elucidated using the Caenorhabditis elegans. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging effect of Q37 showed similar potent activities in comparison with vitamin C. Q37 also showed potent superoxide quenching activities as measured by the riboflavin- and xanthine-originated superoxide quenching activity tests. Q37 prolonged lifespan of worms under normal culture condition. In terms of protective effect of Q37 on the stress conditions such as thermal and oxidative stresses, Q37-treated worms exhibited enhanced survival rate, as compared to control worms. To know the possible mechanism of Q37-mediated increased lifespan and stress resistance of worms, we examined the activities of Q37on superoxide dismutase (SOD), and invested intracellular reactive oxygen species (ROS) levels. The results revealed that Q37 was able to elevate SOD activity of worms and reduce intracellular ROS accumulation in a dose-dependent manner.

• Biomolecules & Therapeutics
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• 제13권4호
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• pp.220-226
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• 2005

The present study was performed to evaluate combination effect of 7$\beta$-hydroxycholesterol (7$\beta$-OHC) and $\gamma$-radiation in NCI-H460 human lung cancer cells. 7$\beta$-OHC in combination with $\gamma$-irradiation has an enhanced effect in decreasing clonogenic survival and increasing cellular DNA damage. Pretreatment of cells with 7$\beta$-OHC enhanced radiation-induced apoptosis. Apoptosis of the cells by combined treatment of 7$\beta$-OHC and $\gamma$-irradiation was associated with reactive oxygen species generation and loss of mitochondrial membrane potential, resulting in the activation of caspase 9 and caspase 3. The combined treatment also resulted in an increased G1 cell cycle distribution. These results indicate that 7$\beta$-OHC shows the additive effect of radiation sensitivity in human lung carcinoma cells in vitro.