• 한국균학회지
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• 제52권1호
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• pp.35-54
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• 2024

With a history spanning 9,000 years, yeast has played a central role in the traditional production of fermented foods, particularly alcohol and bakery. Recent research has highlighted the versatility of yeast in various industries, including the production of bioactive compounds, bioethanol, pigments, and enzymes, and as a host for molecular biology studies. In Korea, yeast isolation has traditionally focused on traditional fermented foods such as soybean paste. However, there is a growing trend in the isolation and characterization of yeasts from natural environments such as flowers and fruits for industrial applications. In this study, we isolated and identified 12 yeasts from various natural environments in Korea, including botanical gardens and parks that are not listed on the National Species List of Korea (NSLK). These newly discovered species included Sakaguchia oryzae, Cystobasidium raffinophilum, Meira argovae, Kazachstania humilis, Meyerozyma smithsonii, Anthracocystis trispicatae, Naganishia brisbanensis, Tremella yokohamensis, Kwoniella shandongensis, Kwoniella newhampshirensis, Aureobasidium proteae, and Rhodotorula dairenensis.

• Journal of Species Research
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• 제13권3호
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• pp.310-317
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• 2024

Various samples from island and coastal ecosystems in South Korea were investigated to discover unrecorded bacterial species. Soils from these areas, along with seawater samples, were plated on marine agar and R2A agar (containing 3% sea salt). From these samples, approximately 1,070 bacterial strains were isolated as single colonies and identified using 16S rRNA gene sequences. A total of 20 strains, which exhibited at least 98.7% similarity in their 16S rRNA gene sequences to those of validly published bacterial species not yet reported in Korea, were identified as unrecorded bacterial species. These strains belonged to three phyla, six classes, 10 orders, 14 families, and 16 genera. These were assigned as follows: Thioclava, Breoghania, Acidovorax, Erythrobacter, Paracoccus, Jiella, Aurantimonas, and Qipengyuania within the class Alphaproteobacteria; Pseudomonas, Cobetia, and Rheinheimera within the class Gammaproteobacteria; Aequorivita, Leeuwenhoekiella, and Polaribacter within the class Flavobacteriia; Algoriphagus within the class Cytophagia; and Microbacterium within the class Actinobacteria. The unreported species underwent further taxonomic characterization, which included assessments of Gram reaction, colony and cell morphology, biochemical and phylogenetic characteristics.

• Journal of Forest and Environmental Science
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• 제40권2호
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• pp.82-89
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• 2024

This study aimed to investigate habitat distribution and beetle diversity, using beetles as biological indicator species to assess environmental changes in the Mt. Mangunsan area near the South Sea of Korea. Plots were installed at varying elevations on the southern and northern slopes of Mt. Mangunsan, and the beetle species composition was determined. A total of 1,368 beetles comprising 32 species belonging to ten families were collected between May and September 2023 from the study sites in the Mt. Mangunsan area near the South Sea of Korea. The two most prevalent species consisted of Synuchus nitidus, with 152 collected beetles, and Calosoma chinense, with 128 collected beetles. In May, June, July, August, and September, 76 beetles comprising 10 species, 180 beetles comprising 18 species, 138 beetles comprising 15 species, 525 beetles comprising 27 species, and 449 beetles comprising 25 species were collected, respectively, with the highest abundance observed in August. In terms of elevation, 239 beetles comprising 19 species were collected at 300 m; 352 beetles comprising 27 species at 400 m; 314 beetles comprising 24 species at 500 m; 280 beetles comprising 22 species at 600 m; 183 beetles comprising 16 species at 700 m. Based on the slope, 442 beetles comprising 18 species belonging to eight families were collected on the southern slope, and 926 beetles comprising 24 species belonging to 10 families were collected on the northern slope. Analysis of the beetles on the southern slope produced the following estimations: diversity of 1.086, evenness of 0.866, and dominance of 0.109. Meanwhile, those in the northern slope yielded a diversity of 1.204, evenness of 0.873, and dominance of 0.081.

• 한국환경복원기술학회지
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• 제15권6호
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• pp.1-16
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• 2012

The research has analysed the targeted land situation, the composition of species, legally controlled plants, vegetation distribution, dominance, species diversity, and the similarity to evaluate the plant characterization of the eco forest in Jangsan, Busan. The results of the research is as following. The number of the plants that belongs to the area is 63 families, 126 genuses, and 163 species. Among them the fern plants : 4 families, 5 genuses, 5 species, and the egg species : 5 families, 8 species, 10 species, and angiospermae : 54 families, 113 genuses, 148 species have been checked. Monocotyledoneae of angiospermae : 7 families, 20 genuses, 22 species (13.5%) and dicotyledoneae : 47 families, 93 genuses, 126 species (77.3%) have been totally checked. The endangered wildlife species by the standard of the Ministry of Environment and the rare plants by the standard of IUCN evaluation realesed by the Bureau of Forest and National Plant Institution has never been checked but one species of Weigela subsessilis has been checked as an approving plant in being delivered abroad, the 8 imported plant by the standard of National Plant Institution checked, some disturbing ecosystem plants released by the Ministry of Environment (2009) not emerged. The succession into the oak trees is occurring to the biodiversity of the researched area, the number of species and their species diversity.

• 수산해양기술연구
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• 제43권3호
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• pp.206-211
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• 2007

The sciaenid species, which has swimbladder, generates sounds by vibrating in its length wards when spawning or getting stimulus from something else. This research analyzed on sound frequency characteristics relatives to their swimbladder length of three sciaenid species, yellow croaker(Larimichthys polyactis), brown croaker(Miichthys miiuy), and white flower croaker(Nibea albiflora). As results, the dominant frequency for yellow croaker ranged over 38.08 to 141.60Hz in wide frequency band, and the averaged pulse duration expressed in $280.3{\pm}156.0ms$. For brown croaker, the dominant frequency ranged in 49.80 to 59.57Hz, and the averaged pulse duration was $129.1{\pm}36.9ms$. Moreover, the dominant frequency of white flower croaker ranged in 73.24 to 86.91Hz, and the averaged pulse duration was $88.0{\pm}15.9ms$, it has shorter pulse duration than any two species. Therefore, the dominant frequency relatives to swimbladder length of sciaenidae showed that it had widely resonant characteristics and long pulse duration as in shorter swimbladder length. Additionally, for white flower croaker, we could confirm their behavior and sounds in response to production of recorded sounds using underwater speaker.

• 한국환경과학회지
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• 제8권6호
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• pp.711-716
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• 1999

This research explored the feasibility of preparing and utilizing a prefonned polymeric solution of Al(III) for coagulation in water treatment. Slow base(NaOH) injection into supersaturated aluminum chloride and aluminum sulfate solutions did produce high yields of Al polymers useful to water treatment applications. The method of characterization analysis was based on timed spectrophotometer with ferron as a color developing reagent. The hydrolytic Al species were divided into $monomeric(Al_a),\;polymeric(Al_b),\;and\;precipitate(Al_c)$ from the difference in reaction kinetics. The analysis of PACl's characteristics showed that the quantity of polymeric Al produced at value of$ r(OH_{added}/AI)=2.2$ was $83\%$ of the total aluminum in solution, as showing maximum contents and precipitated Al was dramatically increased when r was increased above 2.35. In addition, the characteristics of polyaluminum sulfate (PAS) showed that polymeric Al contained at r = 0.75 was $18\%$ of the total aluminum in solution. The synthesized PACI and PAS were stable during storing period, as indicating negligible aging effect. The effect of sulfate ion on PACI was dependent on the concentration of sulfate ion. That is, polymeric species decrease and precipitate species increase as sulfate ion concentration increased. It can be concluded that the sulfate cause the formation of $Al(OH)_{3(S)}$ at low pH. However, The effect of calcium ion was negligible for distribution of Al species.

• Mycobiology
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• 제31권2호
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• pp.74-80
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• 2003

Isolates of Trichoderma spp. collected from Pleurotus ostreatus and P. eryngii beds, which included loosened substrate compactness and development of green colour, were grouped into three species. The occurrence of different species of Trichoderma was as T. cf. virens(70.8%), T. longibrachiatum(16.7%) and T. harzianum(12.5%). The conidia of Trichoderma spp. were ellipsoidal, obovoid and phialides were bowling pins, lageniform and the length of phialides was $3.5{\sim}10.0{\times}1.3{\sim}3.3{\mu}m$. Phialides of T. cf. virens and T. harzianum were tending clustered, but it was solitary disposition in T. longibrachiatum. T. cf. virens was characterized by predominantly effuse conidiation, sparingly branched, and fertile to the apex and it was penicillate type. RAPD analysis could detect variability amongst three different species of Trichoderma using two newly designed URP-primers. However, intra-specific variation could not be detected in all the isolates except for rDNA sequence data classified Trichoderma isolates into three distinct groups representing three species. The profiles of rDNA sequences of isolates representing a species showed high similarity in T. cf. virens and T. harzianum. However, there was a variation in rDNA sequences of isolates representing T. longibrachiatum. The results of present study reveals that molecular techniques of RAPD and rDNA sequencing can greatly aid in classification based on morphology and precise identification of fast evolving species of Trichoderma.

• Asian-Australasian Journal of Animal Sciences
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• 제22권1호
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• pp.131-138
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• 2009

Among rumen microbes, bacteria play important roles in the biological degradation of plant fiber due to their large biomass and high activity. To maximize the utilization of fiber components such as cellulose and hemicellulose by ruminant animals, the ecology and functions of rumen bacteria should be understood in detail. Recent genome sequencing analyses of representative fibrolytic bacterial species revealed that the number and variety of enzymes for plant fiber digestion clearly differ between Fibrobacter succinogenes and Ruminococcus flavefaciens. Therefore, the mechanism of plant fiber digestion is also thought to differ between these two species. Ecology of individual fibrolytic bacterial species has been investigated using pure cultures and electron microscopy. Recent advances in molecular biology techniques complement the disadvantages of conventional techniques and allow accurate evaluation of the ecology of specific bacteria in mixed culture, even in situ and in vivo. Molecular monitoring of fibrolytic bacterial species in the rumen indicated the predominance of F. succinogenes. Nutritive interactions between fibrolytic and non-fibrolytic bacteria are important in maintaining and promoting fibrolytic activity, mainly in terms of crossfeeding of metabolites. Recent 16S rDNA-based analyses suggest that presently recognized fibrolytic species such as F. succinogenes and two Ruminococcus species with fibrolytic activity may represent only a small proportion of the total fibrolytic population and that uncultured bacteria may be responsible for fiber digestion in the rumen. Therefore, characterization of these unidentified bacteria is important to fully understand the physiology and ecology of fiber digestion. To achieve this, a combination of conventional and modern techniques could be useful.

• Mycobiology
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• 제48권3호
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• pp.210-218
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• 2020

Peach (Prunus persica L.) is one of the major fruit crops in South Korea, along with apple, persimmon, and Asian pears. Peach anthracnose is a continuing threat to growers and is accountable for enormous economic loss. In July 2018, anthracnose of peach appeared at different peach orchards in Gyeongsangbuk-do region, Korea. The typical anthracnose symptoms (brown, circular, and necrotic lesions) were observed on the fruits. Anthracnose of peach was surveyed in different peach orchards of Gyeongsangbuk-do, and 20 fungal isolates from 19 diseased fruits were collected. Multigene phylogenetic analyses coupled with morphological characteristic analysis approaches were used for identifying the fungal species isolated from diseased fruits. This study confirmed three Colletotrichum species. Based on the results, Colletotrichum siamense are reported for the first time as causal agents of peach anthracnose alongside C. fructicola and C. fioriniae, which has been reported previously. Pathogenicity assays were performed for the three isolates representing all the species identified, and Koch's postulates on detached healthy peach fruits were verified. All the identified species were pathogenic on peach fruits as the typical anthracnose symptoms were reproduced. Significant variations in the virulence were observed among fungal species on peach fruit.

• The Plant Pathology Journal
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• 제21권3호
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• pp.221-228
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• 2005

A total of 179 isolates of Trichoderma spp. were collected from oyster mushroom substrates in Korea. On the basis of morphological and cultural characteristics, Trichoderma isolates were divided into seven groups, namely T. atroviride, T. citrinoviride, T. harzianum, T. longibrachiatum, T. virens, and two unidentified species, referred to as Trichoderma sp. 1 and 2. The predominant species was Trichoderma sp. 2 (n=86) followed by Trichoderma sp. 1 (n=52). Trichoderma sp. 1 and 2 were morphologically distinct not only from the other species of Trichoderma reported but also from each other in the characteristics such as mycelial growth rate, colony appearance, shape of conidia and conidiophores and branching pattern of phialides, although branching pattern of phialides of Trichoderma sp. 1 was similar to that of T. harzianum. In virulence test, the degree for compost colonization of Trichoderma sp. 2 was significantly greater than that of the other Trichoderma species. Trichoderma sp. 2 was found to be the main cause of green mold disease in oyster mushroom production. More work including molecular characterization is needed to confirm the species of Trichoderma sp. 1 and 2.