• Applied Biological Chemistry
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• 제30권4호
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• pp.328-334
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• 1987

대두(大豆) 및 대두가공품첨가(大豆加工品添加)에 따른 유지안정성(安定性)을 검토하기 위하여 대두유(大豆油)에 대두(大豆) 및 대두가공품(大豆加工品)의 용매추출물(抽出物)을 첨가하고 또한 Ramyon을 제조할 때 대두(大豆) 및 대두가공품(大豆加工品)을 첨가하여 그 유지(油脂)의 산가(酸價), 과산화물가(過酸化物價), TBA가(價)를 측정(測定)하였다. 1. 대두유(大豆油)에 대한 항산화효과(抗酸化?果)는 분리대두단백추출물(分離大豆蛋白抽出物)이 가장 크고 탈지대두추출물(脫脂大豆抽出物), 대두열수추출물(大豆熱水抽出物)이 그 다음가고 탈지대두(脫脂大豆) Ethanol추출물(抽出物)도 유효(有效)하였으나 대두냉수추출물(大豆冷水抽出物)은 효과(?果)가 거의 없었다. 2. Ramyon지질(脂質)에 대하여 저장기간마다 산가(酸價), 과산화물가(過酸化物價) 및 TBA가(價)의 변화는 분리대두단백가수분해물(分離大豆蛋白加水分解物)이 가장 변화(變化)가 적었고 탈지대두가수분해물(脫脂大豆加水分解物), Tempeh, Tempeh추출물(抽出物), 탈지대두분말(脫脂大豆粉末)첨가 순으로 큰 변화(變化)를 보여 대체적으로, 가수분해물(加水分解物)첨가는 매우 효과적인 산화방지작용(酸化防止作用)을 나타냈다.

• 한국환경보건학회:학술대회논문집
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• 한국환경보건학회 2004년도 International Conference Global Environmental Problems and their Health Consequences
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• pp.182-184
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• 2004

Oral cancer is the sixth most common cancer globally. The effects of several extracts from soybeans and Korean soybean paste (doen-jang) on the growth of human oral carcinoma cells in vitro were assessed. We prepared petroleum ether extract, ethyl acetate extract, chloroform extract, methanol extract, and water extract from soybeans and soybean paste. We used KB cell, which is an oral epidermoid carcinoma cell, and investigated proliferation of the tumor cells using MTT method. Each extract of soybean paste suppressed the KB cell proliferation. A dose-response relationship was observed between the level of ethyl acetate extract of soybean paste and its suppression of the cell proliferation. The effects of soybean extracts were lower than those of soybean paste extracts. The effects might be enhanced by the fermentation of soybeans. The results of this work indicate that extracts from soybeans and Korean soybean paste could have potential as anti-tumor substances.

• 약학회지
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• 제45권3호
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• pp.293-301
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• 2001

Genetically modified organism (GMO) using recombinant DNA technique has been exponentially increased, however there are still arguments for the safety of GM foods. The objective of this research was to compare the allergens of GM soybean(Roundup Ready$^{TM}$) with conventional soybeans. Each soybean extracts were prepared as crude extracts, heated extracts, and as heated and simulated gastric quid (SGF)-digested samples to characterize the stability of allergens to physicochemical treatment. Positive sera from 20 soybean-sensitive patients and control sera from 5 normal subjects were used to identify the endogenous allergens in soybeans. Specific-IgE binding activities to each soybean preparations were evaluated by ELISA and immunoblot technique. In ELISA result, IgE binding activities of positive sera to soy crude extracts generally showed two fold higher mean value than those of control sera, how-ever there was no significant difference between GM soybean and natural soybean varieties. Extracted proteins form each of the soybean preparations were separated with SDS-PAGE. The band pattern of GM soybean was very similar to those of natural soybean varieties. Immunoblots for the different soybeans revealed no differences in IgE-binding protein patterns, moreover, disclosed five prominent IgE-binding bands (75, 70, 50, 44 and 34 kDa) in crude extracts, four (75, 70, 44 and 34 kDa) in heated preparations, one (50 kDa) in heated and SGF-digested preparations. These IgE binding bands were consistent with previously reported results on the soybean. These results indicate that GM soybean (Roundup Ready$^{TM}$) is no different from natural soybean in terms of its allergen.gen.

• Food Science and Biotechnology
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• 제16권3호
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• pp.451-456
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• 2007

The potential antioxidant activities of methanolic extracts from soybean and rice fermented with Monascus sp. were investigated. M. pilosus IFO 480 and M. anka IFO 478 were screened as a suitable strain to promote the antioxidant activities in soybean- and rice- fermentation. The methanol extracts from soybean and rice after fermenting for 20 days at $30^{\circ}C$ resulted in a significant increase in the antioxidant capacities expressed as radical (ABTS and DPPH) scavenging assay and peroxidation inhibition (%) by thiocyanate method and increased (p<0.01) by a 2.6 to 3.1-fold compared with those of the unfermented products. The average antioxidant potentials of Monascus-fermented soybean extracts (MFSE) were significantly (p<0.01) stronger than Monascus-fermented rice extracts (MFRE). A linear correlations between free radical scavenging activity of MFSE and the total phenolics content (r=0.84) and total flavonoids content (r=0.81) were observed. These results indicated that MFSE exhibited stronger (p<0.01) antioxidant activity and contained significantly higher levels (p<0.05) of phenolics than MFRE.

• 한국염색가공학회지
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• 제17권5호
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• pp.20-25
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• 2005

The colorants were extracted from black soybean using a buffer solution. Dyeing properties and the fastness of silk fabric dyed with black soybean extracts were investigated. K/S values of dyed fabric were increased with concentration of black soybean extracts. Magnesium sulfate, nickel sulfate and calcium chloride as mordants are used to obtain reddish purple color. Optimum dyeing temperature of black soybean extracts was $30^{\circ}C$. Fastness were generally good except for light fastness which was extremely poor.

• 한국축산식품학회지
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• 제24권4호
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• pp.405-410
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• 2004

본 연구는 콩 제품 추출물의 항산화 활성을 DPPH 수소 공여 능으로 실험하고, 기존의 항산화제와의 상승효과 및 TBA radical scavenging activity를 실험하였고 그 결과는 다음과 같다. 콩 제품 추출물은 DPPH와 반응하여 시간이 경과함에 따라 감소하고, 시판 항산화제인 ascorbic acid는 반응 초기에 완료되는 결과를 보이는 반면 BHT와 BHA는 지속적으로 감소되는 것을 확인할 수 있었다. 또한 콩 발효식품인 된장과 청국장 추출물이 비 발효식품인 두유 추출물에 비하여 감소폭이 다소 크게 차이가 나타났다. 콩제품 추출물과 시판 항산 화제의 상승효과를 조사한 결과 모든 시험 구에서 반응시간이 경과함에 따라 감소폭이 증가하여 상승효과가 나타났으며 발효 식품인 된장과 청국장이 비 발효 식품인 두유에 비하여 시판 항산화제와의 상승효과를 더욱 강하게 나타내었다. 콩 제품 추출물의 TBA 측정을 조사한 결과, 청국장 추출물의 억제율은 45.8％, 된장추출물의 억제율은 45.2％로 나타났고, 두유 추출물의 억제율은 이보다 약 12％ 낮은 33.6％로 나타났다. 따라서 이들 추출물 중 두유 추출물은 기존의 이 소플라본이 생체에 미치는 긍정적인 효과와 함께 이들 항산화제 기능을 축산식품에 응용 가능한 것으로 사료된다.

• 한국식생활문화학회지
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• 제19권6호
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• pp.587-597
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• 2004

Application of pheasant and soybean extracts to improve the quality of baechu kimchi was attempted. Pheasant and soybean extracts at various combinatory concentrations were added into baechu kimchi and fermented for 24 days at $10^{\circ}C$. Assay was performed on salinity, acidity, bacterial growth, sensory evaluations, and physical properties. The pH decreased, and acidity increased by fermentation time. The optimum pH 4.2 was reached within $12{\sim}15\;days$, and optimum acidity was reached within $6{\sim}9\;days$. The number of lactic acid bacteria was increased by fermentation time. Score of intensity characteristics in color. crispness carbonated taste, and overall quality were higher for the pheasant and soybean extracts added baechu kimchi(B) than for the control product(S). The result of L and a value of baechu kimchi were Higher in treatment than in control as fermentation time increased. The hardness of baechu kimchi with pheasant and soybean extracts(B) were higher than those of control(S).

• 한국식품영양과학회지
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• 제29권2호
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• pp.193-197
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• 2000

The effects of black soybena extracts on enzymes activies of rat were evaluated in present study. Eighty-four male Sprague-Dawley rats weighing 100$\pm$10g were divided into twelve groups which consisted of black soybean extract, Pb and Cd solution, and black soybean extract plus Pb or Cd soln groups. The weight gain was increased in black soybean extracts and Pb soln solution group but decreased in Cd soln solution group. The results obtained form the experiment were as follows: Glutamate pyruvate trasaminase (GPT) and glutamate oxaloacetate oxaloacetate transaminase (GOT) activities were not significantly different among experimental groups. The lactate dehydrogenase (LDH) activities of black soybean extract administered groups were decreased than those of Pb and Cd solution group. Black soybean group increased cholinesterase (ChEase) activity as compared to administration of Pb and Cd soln group.

• 한국미생물·생명공학회지
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• 제24권4호
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• pp.525-528
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• 1996

To investigate the inhibitory effect of soybean and Korean traditional fermented soybean products on the chemically induced mutagenesis, we extracted soybean, Kanjang, Doenjang, Kochujang, and Chongkukjang with water, methanol and hexane. Inhibitory effect of the extracts was assayed by the SOS chromotest using Escherichia coli PQ37 as a test strain. 4-nitroquinoline-1-oxide(4NQO), N-methyl-N'-nitro-N-nitrosoquanidine(MNNG), and aflatoxin B$_{1}$(AFB$_{1}$) were used as mutagens. Methanol extracts showed relatively higher inhibitory effect than water and hexane extracts. Methanol extracts of soybean, Doenjang, Kochujang, and Chonhkukjang showed inhibitory effect of 68.4, 96.3, 17.5, and 100.9% against MNNG, and 28.6, 109.1, 41.3, and 101.8% against AFB$_{1}$, respectively. Doenjang methanol extract showed inhibitory effect of 51.0, 96.3, and 109.1% against 4NQO, MNNG, and AFB$_{1}$, respectively. Methanol extract of Doenjang showed dose-dependent inhibitory effect against 4NQO, MNNG, and AFB$_{1}$. Inhibitory effect of heat-treated Doenjang and Chongkukjang methanol extracts on the mutagenecity of MNNG and AFB$_{1}$ was remained over 95% of the inhibitory effect of heat-untreated extracts, demonstrating the heat stability of the potent antimutagenic activity.

• 한국식품저장유통학회지
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• 제17권6호
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• pp.874-880
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• 2010

검은콩과 검은콩 발효제품을 이용하여 천연보존료나 건강 기능성식품을 개발하기 위하여 검은콩 중 약콩과 서리태를 선정하여 청국장을 제조한 후, 검은콩과 청국장을 물과 에탄올로 추출하여 폴리페놀 함량과 항산화능을 측정하였다. 약콩과 약콩 청국장에 대한 물추출물의 폴리페놀 함량은 각각 316.23 mg/100 g, 896.01 mg/100 g이었으며, 서리태와 서리태 청국장에 대한 물추출물의 폴리페놀 함량은 각각 304.28 mg/100 g, 875.23 mg/100 g으로 검은콩 청국장 물추출물의 폴리페놀 함량은 각각의 검은콩에 비하여 2.8배로 증가하였다. 검은콩으로 제조한 청국장 물추출물의 전자공여능과 SOD 유사활성은 원료의 콩에 비하여 높은 활성을 나타내었다. 검은콩과 검은콩 청국장의 물과 에탄올추출물은 모두 혈전용해능을 나타내었으며 약콩 청국장의 물추출물이 가장 우수한 혈전용해능을 나타내었다. 이러한 결과를 종합해 볼 때, 검은콩에 비하여 검은콩으로 제조한 청국장 추출물에서 폴리페놀 함량이 증가하였으며 항산화능과 혈전용해능이 증가하여 청국장은 건강식품의 개발이나 정상세포의 산화적 손상을 억제할 수 있는 천연항산화제로서 이용될 수 있을 것으로 생각된다.