• Nutrition Research and Practice
• /
• 제2권4호
• /
• pp.218-226
• /
• 2008

The present study evaluated the effects of various dosages of soybean isoflavone extract on lipid profiles, lipid peroxidation and antioxidant activities in streptozotocin-induced diabetic rats. The one normal control group was fed an AIN-76-based experimental diet and four diabetic groups were fed the same diet, supplemented with four different levels of soybean isoflavone extract for seven weeks. The daily dosages of pure isoflavone for four diabetic groups were set to be 0 mg (diabetic control), 0.5 mg (ISO-I), 3.0 mg (ISO-II) and 30.0 mg (ISO-III) per kilogram of body weight, respectively. The plasma total cholesterol levels and the TBA-reactive substances contents in the liver and kidney were significantly lowered in ISO-II and ISO-III groups compared to those in the diabetic control group. The levels of plasma HDL-cholesterol, plasma vitamin A and hepatic superoxide dismutase were significantly increased in those two groups compared with the diabetic control group. The present study demonstrated the possibility that the diets supplemented with 3.0 mg and 30.0 mg of soybean isoflavone extract may have beneficial effects on the plasma lipids, tissue lipid peroxidation and partly on antioxidant system in diabetic animals and there were no significant differences between the ISO-II and ISO-III groups. The results suggest that the effective daily dosage level of isoflavone for improving lipid metabolism in diabetic rats may be above 3.0 mg per kilogram body weight.

• Toxicological Research
• /
• 제32권2호
• /
• pp.141-147
• /
• 2016

We evaluated the antioxidant activity and melanogenic effects of black soybean ethanol extracts, including Rhynchosia nulubilis bean ethanol extract (RNBEE), R. nulubilis leaf ethanol extract (RNLEE), R. volubilis bean ethanol extract (RVBEE), and R. volubilis leaf ethanol extract (RVLEE). The total polyphenol contents of RNBEE, RNLEE, RVBEE, and RVLEE were 16.0, 57.7, 365.9, and 260.1 mg/g, respectively. The total flavonoid contents of RNBEE, RNLEE, RVBEE, and RVLEE were 40.4, 91.7, 84.7, and 216.5 mg/g, respectively. The electron-donating abilities of RNBEE, RNLEE, RVBEE, and RVLEE at $1,000{\mu}g/mL$ were 32.4%, 12.7%, 83.5%, and 84.5%, respectively. RNBEE, RNLEE, RVBEE, and RVLEE at $50{\mu}g/mL$ significantly increased (p < 0.01) melanin contents by 30.4%, 32.1%, 35.5%, and 37.4%, respectively, compared to that of the control. RNBEE, RNLEE, RVBEE, and RVLEE at $50{\mu}g/mL$ significantly increased (p < 0.01) intracellular tyrosinase activity by 18.4%, 21.8%, 21.5%, and 21.1%, respectively, compared to that of the control. These results demonstrated that black soybean ethanol extracts promote melanogenesis in melan-a cells. Among the black soybean ethanol extracts, R. volubilis was found to be more effective than R. nulubilis, and leaf extract was found to be more effective than bean extract. The potential mechanism underlying the hyperpigmentation effects of black soybeans is the promotion of tyrosinase activity.

• 한국식품저장유통학회지
• /
• 제23권1호
• /
• pp.42-48
• /
• 2016

다양한 생리활성을 가진 오미자 추출액을 천연응고제로 이용한 백태 및 서리태 두부의 이화학적 품질 특성 및 관능적 특성을 분석하고 오미자를 활용한 다양한 기능성 두부의 개발 및 기존의 화학응고제에 대한 오미자 추출액의 대체가능성을 비교 평가하였다. 오미자 추출액 첨가 백태 및 서리태 두부의 수분 및 회분 함량은 $MgCl_2$ 첨가구보다 낮았으나, 단백질 및 지방의 함량은 오미자 추출액 첨가구에서 높게 관찰되었으며, 색도는 전반적으로 두부 제조에 사용된 콩의 색깔에 따라 영향을 받은 것으로 확인되었다. pH는 오미자 추출액 첨가구에서 $MgCl_2$ 첨가구보다 높게 나타났으며, 당도 및 염도는 오미자 추출액 첨가구가 대조군보다 낮은 값을 보였다. 아미노산 함량은 모든 백태 및 서리태 시료에서 arginine이 가장 높게 관찰되었으나, 전반적으로 오미자 추출액 첨가구(3.74 및 3.71 mg/100 g)보다 $MgCl_2$ 첨가구(9.01 및 7.99 mg/100 g)에서 더 높은 값을 보였다. 무기질 함량은 백태 및 서리태 두부 모두 Ca, K, Mg, Na의 함량이 두 첨가구 모두에서 높은 값을 보였으며, 대체적으로 $MgCl_2$ 첨가구가 오미자 추출액 첨가구보다 높은 값을 나타내었다. 이들 두부에 대한 관능평가 결과, 모든 시료에서 오미자 추출액 첨가구가 색, 두부 고유의 향기, 두부 고유의 맛, 조직감에서 모두 $MgCl_2$ 첨가구보다 높은 점수를 얻었으며 전체적인 기호도 역시 $MgCl_2$ 첨가구보다 높은 점수를 나타내었다. 본 연구의 결과는 건강 기능성을 가진 오미자 추출액만을 첨가하여 두부를 응고시키고 제조하는 것이 가능함을 보여주고 있으며, 이는 건강에 대한 부정적인 측면을 갖는 화학응고제의 대체재로써 오미자 추출액 천연응고제의 상업적 활용 가능성을 제시하고 있다.

• 한국유기농업학회지
• /
• 제24권3호
• /
• pp.557-570
• /
• 2016

본 시험은 6년생 '신고' 배(Pyrus pyrifolia Nakai)나무의 병해충 유기농자재 개발을 위하여 발효 콩 침출액과 비가림 시설을 통한 생육양상과 방제효과를 2년간 비교하였다. 2013년에는 예비시험으로 노지에서 발효 콩 침출액을 6회 엽면 살포 하였으며 2014년에는 노지와 비가림 시설에서 발효 콩 침출액을 처리하였다. 2013년에 발효 콩 침출액이 대조구보다 잎의 전질소가 0.46% 정도 증가되었고, 칼륨(0.17%), 칼슘(0.19%), 마그네슘 농도(0.06%)도 상승되었지만 2014년에는 비슷한 수준이 관찰되었다. 노지와 비가림 시설 간의 비교에서는 비가림에서 재배된 잎의 전질소, 칼슘, 마그네슘 농도가 높게 나타났다. 과총엽, 잎건물중, 약해, 그리고 신초생장 정지율은 대조구와 콩 침출액 간에 두 해 모두 비슷하였고, 비가림 시설은 잎 건물중을 증가시켰고 약해에 대한 피해가 관찰되지 않았다. 과실품질은 대조구와 콩 침출액 간에 두 해 모두 비슷하였고, 과육경도는 콩 침출액 처리에 의해 증가되었다. 2014년에 비가림 시설은 노지보다 수확 일을 4일 앞당겼고 ha당 수량과 평균과중은 각각 약 7톤과 20 g 이상 증가시켰고 당도도 향상되었다. 처리 2년간 발효 콩 침출액은 잎과 과실의 병해충 방제에 대한 효과는 관찰되지 않았다. 2014년에 노지와 비가림 간의 병해충 비교에서는 비가림에서 배나무 잎의 검은별무늬병 발생이 크게 증가되었고, 과실에서도 붉은별무늬병 발병률은 63.8%로 나타났다. 이는 5월에 강풍과 비바람에 의하여 비가림 시설 내의 습도가 증가하여 이병률이 높아진 것으로 판단되며 이에 대한 친환경 방제법 개발이 요구되었다. 발효 콩 침출액 처리는 배나무 생육에 별다른 영향을 미치지 않아 장기간에 걸친 연구가 필요할 것으로 판단되었다. 비가림은 노지보다 과실생산성을 향상시켰고 수확시기를 앞당겨서 추석이 다소 이른 9월 중하순 이었을 때 효과적인 시설로 사료되었다.

• 대한약침학회지
• /
• 제25권1호
• /
• pp.63-67
• /
• 2022

Black soybean (BS) is widely thought to prevent or alleviate hair loss, which has been demonstrated by in vitro experiments. However, few clinical studies have directly assessed the effect of BS on hair growth. To assess the safety and efficacy of BS, we administered BS extract to 10 adult patients for 3 months without any other hair products and treatments. The number and diameter of hair lost from washing were examined every 4 weeks. In addition, the participants' satisfaction with their hair condition was also measured using the VAS (1 to 10) before and after treatment. The mean number of lost hair was 140.7 (SD 59.4) at baseline and 38.8 (SD 54.4) after 12 weeks, and the diameter of lost hair was 72.4 ㎛ (SD 11.9) at baseline and 80.4 ㎛ (SD 8.8) after 12 weeks, which indicated a statistically significant improvement (p = 0.002, p = 0.022, paired t-test). The satisfaction score was significantly increased from 6.10 (SD 1.2) to 8.2 (SD 1.1) after 12 weeks. These results suggest that BS extract may promote hair growth and prevent hair loss.

• Journal of Applied Biological Chemistry
• /
• 제65권4호
• /
• pp.447-451
• /
• 2022

Phytoestrogenic S-equol production in human gut exclusively depends on the biotransformation of daidzein to dihydrodaidzein (DHD). With a growing demand for the DHD enriched biomaterials, the commercial soybean hypocotyl extract (SHE) was chosen as a substrate for the microbial DHD production by human gut bacterium MRG-1, anaerobic DHD producer. To optimize the production of DHD, anaerobic fermentation conditions, including sterilization time, growth stage of inoculum, and growth media, were investigated. Maximum DHD production (1.2 g/L) was achieved after 48 h incubation when 1% (w/v) of SHE in the 20-min-sterilized Gifu Anaeboic Medium media was inoculated with OD₆₀₀ 0.3-0.4 of MRG-1. This is the first report that crude soy biomaterial, instead of pure compounds, such as daidzin and daidzein, is utilized for the production of the DHD enriched biomaterial.

• 한국식품저장유통학회지
• /
• 제9권2호
• /
• pp.174-178
• /
• 2002

감초추출물의 농도를 100 ppm, 200 ppm, 300 ppm and 400 ppm로 조절하여 콩을 발아시켜 재배한 콩나물의 아미노산, 유기산 및 유리당 함량을 재배 2일, 3일 및 4일째에 분석하였다. 총 아미노산 함량은 100 ppm에서 3일간 재배하였을 때가 가장 높았고, 또한 재배일수가 길어질수록 aspartic acid 함량은 증가하지만, glutamic acid 함량은 오히려 감소하는 경향을 나타내었다. 총 유리당은 GGE 콩나물의 경우 대조군에 비하여 낮은 함량을 나타내었으며, 그러나 재배일수가 증가할수록 그 함량은 많아졌다 Sucrose함량은 재배일수가 길어질수록 감소하였으나, 다른 유리당은 오히려 증가하였다 총 유기산은 재배일수가 증가할수록 많은 함량을 나타내었으며, 200 ppm으로 재배한 콩나물이 가장 많은 함량을 나타내었다. 결론적으로 감초추출물은 콩나물의 품질증진을 위한 아미노산 및 유기산 함량을 증가시키는 발아 및 재배수로서 효과적일 것으로 판단된다.

• 한국식품영양과학회지
• /
• 제28권6호
• /
• pp.1230-1238
• /
• 1999

To identify antimicrobial activities of soybean paste, studies have been carried out with laboratory manufactured soybean paste, traditional and improved type soybean paste purchased on the market. The soybean paste fermented with Aspergillus oryzae and Rhizopus tamari and commercial soybean paste were shown antimicrobial activities for Bacillus cereus, Escherichia coli, Listeria monocytogenes, Staphy lococcus aureus, Streptococcus faecalis, Escherichia coli O157:H7, Bacillus subtilis and Salmonella typhimurium. Non fermented soybean paste did not show antimicrobial activities against Bacillus cereus, Escherichia coli, Listeria monocytogenes, Staphylococcus aureus and Streptococcus faecalis. The extract of soybean paste extracted by 80% methanol showed the highest antimicrobial activities of in ethyl acetate fraction and followed butanol fraction, respectively. Antimicrobial activities of traditional and improved type soybean paste were shown against all tested microorganisms and clear zone length of traditional soybean paste was slightly larger than that of improved type. Components of antimicrobial fractions of soybean paste were guessed peptides, 4 hydroxy benzoic acid, benzoic acid by GC MS.

• 한국식품조리과학회지
• /
• 제33권1호
• /
• pp.113-120
• /
• 2017

Purpose: Lipid autoxidation of a soybean oil-in-water emulsion with high oil content was studied under after basil extract and/or iron addition. Methods: The emulsion consisted of tocopherol-stripped soybean oil (40 g), citrate buffer (60 g, pH 4.0), and/or $FeSO_4$ (0.5 mg) with 75% ethanol extract (200 mg/kg) of basil (Ocimum basilicum). Lipid oxidation was evaluated using headspace oxygen content, hydroperoxide contents, and p-anisidne values of the emulsion. Polyphenol compound retention in the emulsion during oxidation was determined spectrophotometrically. Results: Addition of basil extract significantly (p<0.05) decreased reduced hydroperoxide contents of the emulsion, and iron significantly (p<0.05) increased anisidine values and decreased oxygen contents. Co-addition of basil extract and iron showed significantly (p<0.05) lower reduced hydroperoxide contents in the emulsion than compared to those of the emulsion with added iron and the control emulsion without basil extract nor or iron. During the emulsion oxidation, polyphenol compounds in the emulsion with added basil extract were degraded, but more slowlywhich was slowed degraded in the presence of iron. Conclusion: The iIron increased the lipid oxidation through hydroperoxide decomposition, and basil extract showed antioxidant activity through radical-scavenging and iron-chelation. Polyphenol degradation was decelerated by iron addition, which suggested suggests iron chelation may be more preferred topreferentially activated over radical scavenging in the antioxidant action by of basil extract in the oil-in-water emulsion with high oil content.

• 한국식품조리과학회지
• /
• 제12권4호
• /
• pp.541-546
• /
• 1996

This study was attempted to evaluate quality of retort-pouched conuiment food by adding garlic, ginger, redpepper in cooking soybean paste pot stew. Cooked and storaged retort-pouch soybean paste pot stew with four different levels of garlic, ginger, red-pepper (T$_1$garlic 2%, T$_2$: ginger l％, T$_3$: red-pepper 2%,T$_4$: garlic 2%+ginger 1 ％) was tested for sensory evaluation, pH, TBA value, and microbiological number changes. 1) No systematic increase of total bacteria counts was detected during the storage periods for bowl or retort pouch soybean-paste samples. The inclusions of ginger extract did not pose any microbiological safty problem. 2) Sensory evaluation conducted by fifteen university students as panelists showed that there were significant differences among five samples in color, flavour, and appearance and a notable preference for T$_1$ sample. 3) There was a slowly increase of TBA value during the first 5-7 days of storage in retort-pouch and bowl. The inclusion of ginger extract at 1.0％ level tended to lower TBA values.