• KSBB Journal
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• 제13권5호
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• pp.599-605
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• 1998

Soybean peroxidase was extracted from soybean hulls and purified by ammonium sulfate precipitations (25% and 75% saturation), pl fractionation, and anionic exchange and gel filtration chromatographies (DEAE-Sephadex A-50 and Superose 12). Modlecular weight and pl value were estimated to be ca. 45 kD and 4.2, respectively. Purified soybean peroxidase had an RZ value of 0.43. Compared with horseradish peroxidase, it showed superior thermal and pH stability. Assuming the first-order kinetics, the thermal deactivation rate constant of soybean peroxidase at 80$^{\circ}C$ was about 8 times lower than that of horseradish peroxidase. Deactivation energy was calculated to be 69.3 kcal/mol. Soybean peroxidase showed about 10% higher H2O2 degradation capacity than horseradish peroxidase. Exploiting these advantages, the soybean peroxidase purified from the domestic soybean hull is expected to replace horseradish peroxidase in various applications.

• 한국식품조리과학회지
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• 제10권4호
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• pp.369-375
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• 1994

This study was carried out to investigate the effect of beany flavor elimination and the acceptability of tofu prepared with blanched soybean. The results were as follows: 1. The content and me total protein of soymilk prepared with blanched soybean were decreased comparing with those of soymilk prepared with raw soybean. But the yield of tofu was not changed by blanching the soybean. 2. The beany flavor(hexanal, 1-hexanol, and pentanoI) was decreased but the toasted nutty flavor(ethanol and propanol) were increased by blanching the soybean. 3. By heating of soymilk, most of volatile compounds was disappeared, but ethanol, hexanal and 1-hexanol were remained. The hexanal content of tofu prepared with raw soybean was eleven times higher than that of tofu prepared with blanched soybean. 4. The color of tofu prepared with blanched soybean was more yellower than that of control group. At the lower temperature of blending, the texture of tofu was more smooth and elastic. 5. The tofu prepared with blanched soybean was more tasteful.

• 한국식품영양과학회지
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• 제17권2호
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• pp.85-94
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• 1988

두유의 고삽미와 불쾌취를 제거하기 위한 기초 자료를 얻기 위하여 대두에 NaOH, $NaHCO_3$ 용액을 처리하여 phenol 화합물의 변화를 HPLC로 정량하였고, 두유중의 beany flavor를 GC로 분석하였으며, 두유의 일반성분 및 기호도를 측정한 결과 대두중의 phenol 화합물은 chlorogenic, p-hydroxybenzoic, p-coumaric, ferulic, gentisic acid가 확인되었으며, chlorogenic acid 함량이 가장 높았다. 대두중의 phenol 화합물중 chlorogenic acid는 주로 결합형, 기타 다른 phenol 화합물은 유리형으로 대부분 존재하였다. 대두를 0.1% NaOH용액으로 8시간 침지하였을 때 chlorogenic acid는 85% 이상이 제거되었다. 대두중의 phenol화합물 제거에는 NaOH용액이 효과적이었고, 온도가 높아짐에 따라 제거율도 높아졌으며, 0.1% NaOH용액에게서 $90^{\circ}C$, 1시간처리하였을 때 phenol 화합물은 대부분 제거되었다. 물, 0.1% NaOH 및 0.5% $NaHCO_3$ 용액으로 침지하여 제조한 두유의 일반성분은 처리간에 비슷하였다. NaOH 용액 처리에 의하여 두유의 beany flavor 중 hexanol은 증가하였으나, hexanal, propanal, pentanal은 60% 이상이 제거되었다. NaOH 용액처리, 고온침지처리에 의하여 두유의 yellowness는 증가하였으나 물과 저온 침지처리에서는 whiteness가 증가하였다. 대두를 0.1% NaOH 용액으로 $90^{\circ}C$에서 1시간 처리하여 제조한 두유의 기호도가 가장 좋았다.

• Weed & Turfgrass Science
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• 제5권4호
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• pp.196-202
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• 2016

국내에는 야생콩이 전국적으로 분포하고 있기 때문에 LM콩으로부터의 야생콩으로 유전자 이동으로 인한 교잡후대종에 관한 연구는 국내 콩 다양성 보전과 LMO 안전관리를 위해 매우 중요하다. 따라서 본 연구는 LM콩과 일년생 야생콩인 돌콩의 교잡후대종 종자의 형태적 및 발아 휴면특성을 평가하여 교잡후대종의 잡초화 가능성을 예측하기 위한 기초자료를 제시하고자 수행되었다. 교잡 1세대의 경우 형태적으로 돌콩과 매우 유사하며, 발아휴면특성 또한 모본인 돌콩과 유사하여 휴면성이 매우 클 것으로 예측된다. 교잡 2세대 종자는 형태적 특성과 발아휴면특성이 부모종의 중간적인 특성을 지니며 모본인 돌콩에 보다 근접한 것으로 확인되었다. 특히 F2의 휴면율은 65.5%에 달할 정도로 매우 높아 잡초화 가능성을 시사한다. 국내 농업환경에서 교잡후대종이 잡초화 되려면 11월 이후에 탈립된 종자가 토양 중에서 월동하여 종자 활력을 유지하고, 휴면이 타파된 후 발아하여 자연 생태계에서 다른 재배종 및 야생종들과 경합하여 생존 및 세대진전을 할 수 있어야 한다. 따라서 LM콩 및 야생콩 간 교잡후대종의 명확한 잡초화 가능성은 종자의 월동성, 생육특성 및 종자생산성 평가 등을 추가적으로 수행하여 다각적인 측면에서 면밀히 평가되어야 할 것이다.

• 한국식품영양과학회지
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• 제23권5호
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• pp.837-844
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• 1994

In order to making the good quality soybean cheese, it is prepared byover growing cow's milk added soybean curd with Actinomucor elegans and the nold-overgrown curd soked in salt-brine/ethanol mixture. The physicochemical changes and sensory evaluation were investigated during the aging period. Crude protein, carbohydrate, crude fat ash contents of the pehtze were increased by elapsing the fermentation time, whereas moisture's decreased . Amino-N and ammonia -N contents of cow's milk added pehtze and soybean phetze were increased 17.25%, 7.23% and 16.16%, 8.42% respectively. Total nitrogen content of the pehtze was decreased by elaping the aging time but soaking solution's increased. Free amino acid content of soybean cheese was increased as a result of the proteolytic action of molds. As a result, sulfur containing amino acid such as methionine and cysteine of the cow's milk added soybean cheese were enriched 1.3 times more than the soybean cheese. Flavor, taste and texture of the cow's milk added soybean cheese were higher than soybean cheese.

• Journal of Nutrition and Health
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• 제32권1호
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• pp.110-117
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• 1999

Free radicals formed during the metabolism of environmental chemicals are known to induce mutagenicity, while different types of antioxidants suppress this event. The purpose of this study was to determine the antioxidative and antimutagenic effects of soybean saponins, and to examine the relationship between these two effects for the elucidation of mechanisms involved in the anticarcinogenicity of soybean saponins. Also, antioxidative and antimutagenic effects of soybean saponins were compared with those of kinown antioxidants. For the measurement of antioxidative capacity, soybean saponins, L-ascorbic acid, $\alpha$-tocophoerol, and BHT at concentrations between 005 and 1.0mg/ml were tested for their ability to donate hydrogens and to reduce the formation of thiobarbituric substances(TBARS). Antimutagenic activity was examined using the Ames salmonella test system at concentrations of 600, 900 or 1200ug/ml. Study results showed soybean saponins and all of the other antioxidants tested possessed dose-dependent antioxidative activities. The ability of hydrogen-donation to DPPH was in the order of L-ascorbic acid>$\alpha$-tocopherol=>BHT>soybean saponins. TBARS formation was also inhibited by these compounds, in the order of BHT>$\alpha$-tocopherol=L-ascorbic acid>soybean saponins. Soybean saponins and other antioxidants also showed antimutagenicity in a dose-dependent manner. Especially, soybean saponins and BHT were excellent antioxidants compounds, inhibiting near 80% of the mutagenic effects at a concentration of 1200ug/ml. The correlation coefficients between antioxidative capacity and antimutagenicity for each compund was statistically significant at p<0.05. These results indicate that soybean saponins possess antioxidative and antimutagenic capacities. Also, antimutagenicity of saponins and other antioxidats is partly due to their antioxidative activities.

• 한국식품영양과학회지
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• 제26권3호
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• pp.371-375
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• 1997

The content of genistein and daidzein which were known to be major antioxidative compounds in soybeans were detected by $C_{18}$ reverse phase HPLC. Most of isoflavones in soybeans were detected in the methanol extract but much less amount of isoflavones in the water extract. Among the four different kind of soybeans, the isoflavone content was highest in brown soybean, followed by yellow, small black and black soybean, in the order. These isoflavones were known to be soluble in hot water, which means transfer of isoflavone content was highest in brown soybean, followed by yellow, small black and black soybean, in the order. These isoflavones were known to be soluble in hot water, which means transfer of isoflavones in soy curd into whey during soy curd processing. To identify the change of isoflavone content during processing of soybean curd, soybean curd were made from yellow, brown and black soybean and isoflavone content were determined in each soybean curd, curd residue and whey. Most of soflavones were remained in the whey, it means most of useful antioxidative compounds were wasted. Thus, it is necessary to develop new technology to collect these isoflavones lost during soybean curd processing.

• Journal of Biosystems Engineering
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• 제30권3호
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• pp.141-146
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• 2005

Worldwide consumption of vegetable soybean has been increasing recently, but, in the process of vegetable soybean production threshing and seperation work accounts for about $80\%$ of overall labor. Therefore, developing of the vegetable soybean thresher is necessary to reduce the cost of labor. The main objective of this study is to develop the vegetable soybean thresher which is suitable for domestic circumstances. The threshing and separating performance, operating cost, and field capacity of developed vegetable soybean thresher are investigated and analysed. The results are as follows. The effective field capacity of the developed vegetable soybean thresher was shown as 4.8hr/10a, and reduced as much as 11.7 times compared with human labor. The ratio of unthreshed soybean-pod to stem after threshing work was shown as $1.5\%$ and the damaged pod ratio of detached soybean was shown as $1.8\%$. The cost of human labor was shown as 2,560,000 won/ha, but the operating of the developed vegetable soybean thresher was shown as 503,000won/ha. If the vegetable soybean thresher would be used in our farm, the minimum cultivation area appeard to be 22.7a for the cost effective management.

• 동아시아식생활학회지
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• 제20권6호
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• pp.909-915
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• 2010

In order to determine the most effective ratio of black soybean powder as an ingredient in cake, black soybean power was added at ratio of 10, 20, 30, and 40% versus wheat flour set as 0(control). Black soybean powder consisted of moisture(8.44%), crude protein(31.34%), crude fat(6.64%), crude ash(3.88%) and carbohydrates(49.70%). The specific gravity, spreadability, and baking loss increased according to the amount of black soybean powder, although specific volume decreased. The chromaticity 'L' and 'b' values of sponge cake with black soybean powder showed an reducing trend while the 'a' value displayed an increasing trend with an increase in black soybean powder. The texture properties of sponge cake with added black soybean powder showed an increasing trend in hardness and stiffness when the cake contained more black soybean powder. The sensory test for sponge cake with added black soybean powder showed high preference for 20% added black soybean powder to sponge cake.

• 한국식품조리과학회지
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• 제22권5호통권95호
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• pp.583-590
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• 2006

This study was carried out to investigate the acceptable ratio of Sulgidduk with added soybean curd residue powder. The moisture content of Sulgidduk with added soybean curd residue powder (0${\sim}$ 10%) ranged from 40.54 ${\sim}$41.38%, and there were no significant differences between the addition of soybean curd residue powder and control. There were also no significant differences in swelling power and pore ratio from control to the addition of 4% soybean curd residue powder. However, these decreased with increasing addition of soybean curd residue powder of more than 6%. The L (lightness) value decreased with increasing addition of soybean curd residue powder, but the a (redness) and b (yellowness) values increased significantly. As the amount of soybean curd residue powder increased, the hardness, cohesiveness, springiness and gumminess decreased, and, the decrease was especially significant with addition of more than 6%. With increases in the storage period, the hardness, springiness and gumminess increased, while cohesiveness decreased for all additions of soybean curd residue powder. In sensory evaluations, Sulgidduk with the addition of 4% soybean curd residue powder was the most preferred with regard to overall quality. These results indicated that the Sulgidduk with 2% and 4% added soybean curd residue powder exhibited the best quality.