• Journal of Plant Biology
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• v.38 no.1
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• pp.39-45
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• 1995

Glycine soja Sieb. et Zucc., a predominantly selfing annual, has been served as a reservoir of germplasm for soybean, G. max (L.) Merr., cultivar improvement. This study describes the levels and distribution of genetic variation within and among 22 Korean populations of G. soja using starch gel electrophoresis. The species maintains very similar levels of genetic variability within populations observed in most other annuals. At the population level, the mean percent of polymorphic loci (P) was 32.6%, mean number of allele per locus (A) was 1.32, and mean expected heterozygosity (He) was 0.112. In addition, total genetic diversity (HT) calculated only for polymorphic loci was 0.347. However, significant differences in allele frequencies among populations were found for all loci (P<0.001 in each case) and, on average, about 70% of the total variation in the species is common to all populations. Indirects estimate of the number of migrants per generation (Nm=0.58, calculated from mean GST) indicates that gene flow is low among Korean populations of the species. In addition, analysis of fixation indices revealed a substantial heterozygote deficiency in most populations and at all loci. This indicates that most populations sampled may have been substructed largely due to inbreeding (predominantly selfing) and restricted gene flow, coupled with founder effect and genetic drift. Considering a high genetic divergence among populations, it is recommended that several Korean populations of the species should be preserved, especially such as populations in the eastern and southeastern Korean peninsula with high variation.

• Development and Reproduction
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• v.19 no.4
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• pp.235-242
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• 2015

Black soybean teata is helpful to preventing obesity through enhancing energy expenditure and suppressing accumulation in mesenteric adipose tissue. The ethanol testa-extract of Cheongja #3 black soybean (ETCBS) is also have similar effects on obesity. So far, it is not clear whether the ethanol testa extract of black soybean can have effect on the characters of subcutaneous adipose stem cells such as proliferation, activity, and adipogenicity. The doubling time was different between subcutaneous adipose-derived stem (ADS) and visceral ADS cells. By the in vitro culture and passage, the doubling time was increased both of them. The shape was not different between groups and their passages were not cause the change of shapes. In the case of visceral ADS cells, the doubling time was 62.3 h or 40.3 h in control or high fat diet administrated mice, respectively, but not modified in subcutaneous ADS cells. ETCBS administration caused of increased the doubling time from 62.3 h to 84.2 h. ETCBS had suppressive effects on the cellular activity of subcutaneous ADS cells. The intensity of Oil Red O staining was very faint in 100 and $200{\mu}g/mL$ ETCBS treated groups. The amounts of accumulated triglyceride were also significantly low in 100 and $200{\mu}g/mL$ treated groups. From these results we know that the doubling times and the effects of ETCBS are different by the anatomical origin of ADS cells. It also suggested that ETCBS may suppress the differentiation of subcutaneous ADS cells into the precursors and maturing of adipocytes.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.5
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• pp.569-577
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• 1996

This experiment was conducted to investigate the effects of water potential by PEG treatment on germination and quantitative changes of seed storage reserves of soybean [Glycine max (L.) Merr.], peanut(Arachjs hypogaea L.) and corn(Zea may L.). Water potential of PEG(M.W. 10, 000) solution as germination media was 0.0, -0.2, and -0.5MPa. The highest moisture uptake rate was found in soybean seedlings among three crops. Moisture content of seedlings of three crops was decreased at -0.5MPa treatment and seedling length was delayed with water potential decrement. As water potential decreased, decreasing rate of protein content of the seedlings compared to seeds was declined in soybean and peanut. Decreasing rate of starch content of the seedlings was decreased in corn at -0.5MPa treatment. Increasing rate of sugar content of the seedlings was markedly decreased at -0.5MPa treatment in all crops. The results of this experiment showed that availability of moisture and synthesis of sugar for seed germination were influenced below -0.5MPa water potential in three crops.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.2
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• pp.161-168
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• 2013

Soybean cyst nematode (Heterodera glycines Ichinohe) is one of the serious soybean [Glycine max (L.) Merr.] pests in major soybean producing countries. The objective of this study was to investigate of Heterodera glycines type using the five SCN infested soybean field soils and was to evaluate resistance to the soybean cyst nematode HG 2.5.7 type on soybean varieties and germplasms. The five SCN contaminated soil samples were collected from the three provinces on November 2011 in Korea, and eggs were cultured on early spring season in 2012. For the second study, a total fifty nine soybean varieties and germplasms were tested by infestation of HG type 2.5.7 in the greenhouse. Soybean cyst nematode HG types were investigated from five locations, HG 2 (race 1) type at Donghae, HG 2.5 (race 1) type at Jeongseon and Hapcheon, HG type 2.5.7 (race 1 or 5) at Yeongwol, and HG 1.2.7 (race 5) type at Haenam locations in present study. No Korean soybean varieties and germplasms were observed with SCN resistant trait to the HG type 2.5.7. Average SCN female index were calculated with 82.7% in 59 plant materials. Our results could be provided useful information to develop a SCN resistant cultivar in Korea.

• Applied Biological Chemistry
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• v.35 no.6
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• pp.449-456
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• 1992

Expermient were conducted to determine the effect of phosphorus stress on bacteroid content and energy status of soybean (Glycine max [L.] Merr.) nodules. Plants inoculated with Bradyrhizobium japonicum MN 110 were grown with P-stressed (0.05 mM-P) and control (1 mM-P) treatment in the greenhouse. Phosphorus stress decreased nodule mass per plant and nodule mass to whole plant mass ratio. Phosphorus concentration in leaf, stem and root tissues were reduced by 75% but in nodule tissue was reduced only by 40% under phosphorus stress during 3 week experimental period. The bacteroid content per unit nodule mass and the distribution of total nitrogen and total phosphorus among the bacteroid and plant cell fractions of nodule were not affected significantly by phosphorus stress. Regardless of phosphorus treatment, 22% of the nitrogen and 27% of the phosphorus in whole nodules were associated with the bacteroid fraction. The ATP and total adenylate concentrations in and energy charge of whole nodule were decreased 77%, 46% and 37%, respectively, by phosphorus stress. The ATP concentration in and energy charge of the host plant cell fraction of nodules were decreased 86% and 59%, respectively, but these parametres in bacteroid in nodules were not affected by phosphorus stress. These results indicated that nodule is a strong phosphorus sink and that nodule growth and development are regulated to maintain a high phosphorus and energy content in bacteroid even when the host plant is subjected to phosphorus deficiency.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.4
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• pp.409-416
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• 1987

The object of this study was to investigate the differences in dry matter production, physiological characteristic and grain yield of soybean (Glycine max (L.) Merr.) as affected by planting dates in southern region of Korea. This experiment was conducted using four planting dates and four varieties over 3 years (1982-1984). The different responses of soybean varieties to different temperature and daylength, days from planting to flowering were ranged from 43 to 55 days when planted on May 10 long daylength and low temperature condition. Flowering duration was ranged from 12 to 27 days and 9 to 13 days for the plantings made on May 10 and July 9, respectively. Therefore, it was the shortest under the short daylength and high tempera-ture condition. It also appeared that the later planting caused the lower dry matter weight and leaf area index which resulted in lower grain yield. But grain yield was not directly correlated with the unbalance of Xink/Source Ratio and the lodging from excessive vegatative growth. The grain yield was the highest in May 30 and the lowest in July 9 planting: it was ranged from 2.26 to 2.55 t/ha in the former and 0.85 to 1.20 t/ha in the latter, respectively. The increase in yield was depended on the increase in the number of pods per plant, the number of seeds per pod and the seed weight. The distribution ratio of dry matter to leaves was the hightest at the sixth trifoliolate leaf stage and gradually declined thereafter in all planting dates, and it also appeared that the later planting, the lower the ratio of dry matter to leaves.

• Food Science and Biotechnology
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• v.14 no.6
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• pp.778-782
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• 2005

Analysis of black soybeans [Glycine max (L.) Merr.; 59 Korean varieties] revealed that 100-seed weights of green cotyledon seeds (33.5 g, n=31) were higher than those of yellow ones (28.9 g, n=28). Contents of delphinidin-3-glucoside (D3G), cyanidin-3-glucoside (C3G), petunidin-3-glucoside (P3G), and total anthocyanins in seed coats of black soybeans were 0.03-4.15, 0.74-18.36, 0.02-1.60, and 0.87-23.52 mg/g, respectively, among which most prominent anthocyanin was C3G (80.9% of total content), followed by D3G (13.6%) and P3G (5.5%). No significant differences were observed in color parameters $a^*$ and $b^*$ between black soybeans with yellow cotyledon (BYC) and green cotyledon (BGC). Total isoflavone content of BGC was higher than that of BYC, and negative correlation was found between total anthocyanin and isoflavone contents.

• Journal of Applied Biological Chemistry
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• v.44 no.1
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• pp.16-19
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• 2001

Soybean plants(Glycine max [L.] merr.) inoculated with Bradyrhizobium japonicum MN110 were grown in growth chambers under 400 or $800{\mu}l{\cdot}l^{-1}$ atmospheric $CO_2$ and harvested at 25, 28, 32, and 35 DAT to examine the effect of $CO_2$ enrichment on phosphorus accumulation, uptake, and utilization efficiency during vegetative growth. Phosphorus concentration in leaf was lower in high $CO_2$ plant by 47% at 25 DAT and 34% at 35 DAT than those in the control plant but phosphorus concentrations in stem, root and nodule were not affected by $CO_2$ enrichment. Total phosphorus accumulation increased 3.9-fold in high $CO_2$ plant and 3.2-fold in the control plant between 25 and 35 DAT. Elevated $CO_2$ caused a decrease in the whole plant phosphorus concentration by 35%, which was due almost entirely to a decrease in the phosphorus concentration of leaves. $CO_2$ enrichment increased phosphorus utilization efficiency in the whole plant by 70% during the experimental period. Plants exposed to high $CO_2$ had larger root systems than under ambient $CO_2$, but high $CO_2$ plants had lower P-uptake efficiency. Averaged over four harvests, plants at high $CO_2$ had 38% larger root mass that was more than offset the 20% lower efficiency of P-uptake and accounted for increased phosphorus accumulation by high $CO_2$ plant. These results suggest that the reduced phosphorus concentration in soybean plant under $CO_2$ enrichment may be an acclimation response to high $CO_2$ concentration or enhanced starch accumulation, resulting in the plants to have a lower phosphorus requirement on a unit dry weight basis or a high phosphorus utilization efficiency under these conditions.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.4
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• pp.286-291
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• 2002

Seedling establishment of soybean [Glycine max (L.) Merr.] is a critical factor in production system and cultural practices. The objective of this study was to identify the components of soybean seedling developments encompassing planting dates and cultivars that respond to emergence, early growth stage and dry matter accumulation. Three soybean cultivars, Hwangkeumkong, Shinpaldalkong 2, and Pungsannamulkong, were planted at Baegsan silty loam soil. Planting date was May 13, June 3, and June 24 in 2001. Sprinkler irrigation was accompanied with 30mm after seeding for three planting dates. Soybean seedlings were sampled at the growth stages from VE to V5. Days to emergence of soybean seedlings were taken 8 at May 13 and 5 at June 24 plantings. Emergence percentage was over 90 at three planting dates. May 13 planting took 33 days and June 24 planting was 25 days for reaching V5 growth stage. Cotyledon number was decreased after V2. Significant cultivar difference was found in cotyledon dry weight until V2 which differed in seed dry weights at the planting times. Leaf and total dry weights of soybean seedlings were not differed from V1 to V3 among planting dates and cultivars. Leaf water contents were generally ranged 78 to 85%. Branch was appeared from V4. Leaf/stem ratio among cultivars was similar at five growth stages and gradually increased from 2.1 at V1 to 2.8 at V5. The results based on this experiment indicated that seedling establishment of soybean was continued from VE to V3 growth stages affecting mainly by planting date and soil moisture.

• Korean Journal of Weed Science
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• v.15 no.4
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• pp.313-320
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• 1995

The major weeds observed in soybean(Glycine max(L.) Merr.) culture at the paddy field where transplanted rice was cultivated in previous year were Digitaria spp., Echinochloa spp., Chenopodium ficifolium, Rorippa islandica and Stellaria alsine. C. ficifolium and R. islandica increased as soybean was cultivated for two years in the same field. Weed biomass decreased by 84.8% as the seeding date was delayed from April 26 to May 20. Most of weeds started to emerge from 20 days after seeding(DAS) until 40 DAS, and higher seed yield was obtained by eliminating the weeds emerged until 40 DAS. The development of soybean branches, pods stem diameter was severely injured by weed interference, and thus soybean seed yield was reduced by 70% in comparison with a full season weed free plot. Herbicides such as pendimethalin, metolachlor, metolachlor+prometryn and alachlor controlled very effectively weeds present in soybean culture in rice-soybean rotated paddy field.