• Analytical Science and Technology
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• v.30 no.1
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• pp.10-19
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• 2017

Anabolic steroids have similar structures to testosterone, both of which promote the growth of muscle mass and increase strength. However, the side effects of anabolic steroid use may lead to heart attacks or strokes. Additionally, the excessive use of steroids inhibits the production of the sex hormones in the body via a negative feedback loop, which results in testicular atrophy in males and amenorrhea in females. Currently, the method of choice used to test for the presence of anabolic steroids is GC-MS. However, GC-MS methods require chemical derivatization of the steroid sample to ensure compatibility with the analytical method; therefore, analysis of many different samples is difficult and time consuming. Unlike GC-MS, the liquid chromatography-quadrupole-time of flight mass spectrometry (LC-Q-TOF-MS) method is suitable for many samples. Twenty-two different anabolic steroids were analyzed by LC-Q-TOF-MS with various collision energies (CE). Accurate mass spectral data were obtained using a Q-TOF-MS equipped with an electro-spray ionization source and operated in the positive MS/MS mode for several classes of steroids that are often the targets of testing. Based on the collected data, fragmentation pathways were carefully elucidated. The high selectivity and sensitivity of the LC-Q-TOF-MS instrument combined with these fragmentation pathways offers a new approach for the rapid and accurate screening of anabolic steroids. The obtained data from the 22 different anabolic steroids will be shared with the scientific community in order to establish a library to aid in the screening of illegal anabolic steroids.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.2
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• pp.113-118
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• 2011

This study was aimed to develop blend films by rice by-product (rice-hull and rice-bran) and bio-degradable materials. The rice by-product was firstly prepared from the pulverizing for making fine powder. Bio-degradable materials could be prepared by melting at high temperature. The mixture of the fine powder of rice by-product and melted bio-degradable materials was then blended and cast into films. The obtained films were investigated on their morphology, secondary structures and properties by using SEM, ICP and ASTM, respectively. Mechanical properties and degradability of these films were measured and compared to those of the PE films. Mechanical strength of bio-films was higher than that of PE films, however elongation ratio showed lower percent than that of PE film. In addition, bio-film could be degraded into fragments within 3 months under the field condition of normal upland crop cultivation. Bio-degradable mulching film indicated great potential for agronomic use as a new source of bio-degradable material.

• Restorative Dentistry and Endodontics
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• v.22 no.1
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• pp.358-373
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• 1997

Resin modified glass ionomers were introduced in 1988 to overcome the problems of moisture sensitivity and low early mechanical strength of conventional glass ionomers and to maintain their clinical advantages. The purpose of this study was to evaluate the color stability of four resin modified glass ionomers(Fuji II LC, Vitremer, Dyract and VariGlass), one resin composite material(Z-100), and one conventional glass ionomer(GC Fuji II) under several conditions. These conditions were as follows: 1) before curing, 2) after curing, 3) after polishing, 4) after 500 thermocycling, 5) after 1,000 thermocycling, 6) after 1,500 thermocycling and 7) after 2,000 thermocycling. Three specimens of each material/shade combination were made. Materials were condensed into metal mold with a diameter of 10 mm and a thickness of 2.0 mm, and were pressed between glass plates. The material was polymerized using a light polymerizing unit(Visilux II, 3M, USA). After removal of excess, the surface was polished sequentially on wet sandpapers. A reflection spectrophotometer(Model TC-6FX, Tokyo Denshoku Co., Japan) was used to determine CIELAB coordinates($L^*,a^*$ and $b^*$) of each specimen. CIE standard illumination C was used as the light source. The results were as follows : 1. In comparing different shades of same material, CIELAB color difference(${\Delta}E^*$) value was not significantly different from each other(p>0.05). 2. CIELAB color difference(${\Delta}E^*$) values between after-curing and after-polishing were ranged from 5.53 to 27.08. These values were higher than those of other condition combinations. 3. CIELAB color difference(${\Delta}E^*$) values between before-thermocycling and after-thermocycling were ranged from 1.40 to 7.81. Despite the number of thermocycling increased, CIELAB color difference(${\Delta}E^*$) value was low. 4. The color stability of resin modified glass ionomers was more stable than that of conventional glass ionomers but less stable than that of Z100.

• Fire Science and Engineering
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• v.24 no.4
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• pp.12-17
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• 2010

The purpose of this study is to determine how effectively waste tire recycled material mixed with flame retardant work in combating fire. As discovered in the previous study, waste tire mixed with cement mortar has more insulation capacity. However, this mortar is weak against fire. Therefore flame retardant, with a specific proportional mix, will be added to increase its fire prevention capacity. Tests will be made in accordance with ISO 5657 procedures for measuring fire ignition time, flame and shape variation of test pieces at the Building Material Test Institute. The test piece will be set up with horizontal levels having a constant radiation heat of $1{\sim}5W/cm^2$. Temperature transfers and increases from the surface into the interior. Combustible gases result due to pyrolysis, and regular contact is maintained between the fire source and the center of the test piece for assessment purposes. Ignition has not been occurred without adding retardant meaning that there is almost no possibility of ignition of waste tire particle. This fact can be considered as fire load to appreciate a volume of combustion materials. Flame is not occurred due to heat-absorbing effect by adding non-organic series retardant into waste tire particle. Conclusions have been summarized as follows; 1) Combustion of building material can be decreased by adding retardant to waste tire-mixing mortar. But compressive strength and insulation capacity of the material should be measured later. 2) Firing prevention and ignition are main points of building fire. Reasonable fire engineering assessment of interior material should be made for establishing effective disaster prevention system.

• Microbiology and Biotechnology Letters
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• v.38 no.3
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• pp.335-339
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• 2010

A diesel-degrading bacterium, Gordonia sp. SD8, was isolated from soil contaminated with petroleum, and its diesel degradation was characterized in a soil as well as a liquid culture system. SD8 could grow in the mineral salt medium supplemented with diesel as a sole carbon and energy source. The maximum specific growth rate ($0.67{\pm}0.05\;d^{-1}$) and diesel degradation rate ($1,727{\pm}145$ mg-TPH $L^{-1}\;d^{-1}$) of SD8 showed at 20,000 mg-TPH $L^{-1}$ and $30^{\circ}C$, and then this bacterium could degrade high strength of diesel of 40,000 mg-TPH $L^{-1}$. The residual diesel concentration in the inoculated soil with SD8 was 3,724 mg-TPH kg-dry $soil^{-1}$ after 17 days, whereas the diesel concentration in the non-inoculated soil was $8,150{\pm}755$ mg-TPH kg-dry $soil^{-1}$. These results indicate that Gordonia sp. SD8 can serve as a promising microbial resource for the bioremediaion of contaminated soil with petroleum hydrocarbons including diesel.

• Journal of Radiation Protection and Research
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• v.29 no.2
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• pp.129-139
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• 2004

Recently, much attentions are paid to the risk associated with increased uses of medium size radiation sources in medical and industrial fields. In this study, radiation risks to the worker and to the general public due to $^{99m}Tc$ generator were assessed for both normal and accident conditions. Based on the event tree technique, exposure scenarios for various situations were derived. Uncertainty analysis based on the Monte-Carlo technique was applied to the risk assessment for workers and members of the public in the vicinity of the work place. In addition, sensitivity analysis was performed on each of the five independent input parameters to identify importance of the parameters with respect to the resulting risk. Because the frequencies of normal tasks are fat higher than those of accidents, the total risk associated with normal tasks were higher than the accident risk. The annual dose due to normal tasks were $0.6mSv\;y^{-1}$ for workers and $0.014mSv\;y^{-1}$ for public, while in accident conditions $3.96mSv\;y^{-1}\;and\;0.0016mSv\;y^{-1}$, respectively. Uncertainty range of accident risk was higher by 10 times than that of normal risk. Sensitivity analysis revealed that source strength, working distance and working time were crucial factors affecting risk. This risk analysis methodology and its results will contribute to establishment of risk-informed regulation for medium and large radioactive sources.

• Journal of Korean Society of Forest Science
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• v.71 no.1
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• pp.45-49
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• 1985

Protoplast-source meterial and enzyme strength had a significant influence on protoplast yield from hybrid poplar, Populus alba ${\times}$ P. grandidentata. The yield of protoplasts from in vitro culture of 1 month-old plantlets was more than that from greenhouse grown 4 month-old stock plant. In vitro cultured plantlets regulary produced more viable protoplasts with E-I enzyme solution (0.5% cellulase and 0.1% macerase) than those with E-II enzyme solution (1.0% cellulase and 0.2% macerase) after overnight incubation. The mean yield of protoplasts from in vitro cultured plantlets was $4{\times}10^6$ with E-I enzyme solution. Cell division was observed in these protoplast cultures after 7-10 days. Protoplast-derived hybrid poplar cells survived over 3 weeks in culture and some continuous cell divisions were evident. Other aspects associated with protoplasts from in vitro cultured plantlet are also discussed.

• Korean Journal of Community Nutrition
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• v.24 no.6
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• pp.525-534
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• 2019

Objectives: Reduced glucose utilization in the main parts of the brain involved in memory is a major cause of Alzheimer's disease, in which ketone bodies are used as the only and effective alternative energy source of glucose. This study examined the effects of a low-carbohydrate and high-fat (LCHF) diet supplemented with a ketogenic nutrition drink on cognitive function and physical activity in the elderly at high risk for dementia. Methods: The participants of this study were 28 healthy elderly aged 60-91 years showing a high risk factor of dementia or whose Korean Mini-Mental State Examination (K-MMSE) score was less than 24 points. Over 3 weeks, the case group was given an LCHF diet with nutrition drinks consisting of a ketone/non-ketone ratio of 1.73:1, whereas the control group consumed well-balanced nutrition drinks while maintaining a normal diet. After 3 weeks, K-MMSE, body composition, urine ketone bodies, and physical ability were all evaluated. Results: Urine ketone bodies of all case group subjects were positive, and K-MMSE score was significantly elevated in the case group only (p=0.021). Weight and BMI were elevated in the control group only (p<0.05). Grip strength was elevated in all subjects (p<0.01), and measurements of gait speed and one leg balance were improved only in the case group (p<0.05). Conclusions: We suggest that adherence to the LCHF diet supplemented with a ketogenic drink could possibly influence cognitive and physical function in the elderly with a high risk factor for dementia. Further, we confirmed the applicability of this dietary intervention in the elderly based on its lack of any side effects or changes in nutritional status.

• Journal of Life Science
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• v.27 no.12
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• pp.1403-1409
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• 2017

In this study, the xylitol dehydrogenase (XYL2) gene was expressed in Saccharomyces cerevisiae as a host cell for ease of use in the degradation of lignocellulosic biomass (xylose). To select suitable expression systems for the S.XYL2 gene from S. cerevisiae and the P.XYL2 gene from Pichia stipitis, $pGMF{\alpha}-S.XYL2$, $pGMF{\alpha}-P.XYL2$, $pAMF{\alpha}-S.XYL2$ and $pAMF{\alpha}-P.XYL2$ plasmids with the GAL10 promoter and ADH1 promoter, respectively, were constructed. The mating factor ${\alpha}$ ($MF{\alpha}$) signal sequence was also connected to each promoter to allow secretion. Each plasmid was transformed into S. cerevisiae $SEY2102{\Delta}trp1$ strain and the xylitol dehydrogenase activity was investigated. The GAL10 promoter proved more suitable than the ADH1 promoter for expression of the XYL2 gene, and the xylitol dehydrogenase activity from P. stipitis was twice that from S. cerevisiae. The xylitol dehydrogenase showed $NAD^+$-dependent activity and about 77% of the recombinant xylitol dehydrogenase was secreted into the periplasmic space of the $SEY2102{\Delta}trp1/pGMF{\alpha}-P.XYL2$ strain. The xylitol dehydrogenase activity was increased by up to 41% when a glucose/xylose mixture was supplied as a carbon source, rather than glucose alone. The expression system and culture conditions optimized in this study resulted in large amounts of xylitol dehydrogenase using S. cerevisiae as the host strain, indicating the potential of this expression system for use in bioethanol production and industrial applications.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.35 no.8B
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• pp.1197-1203
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• 2010

A strength of BitTorrent, which is widely used for file sharing today, is due to its peer selection mechanism which is designed to encourage peers to contribute data. In the peer selection phase in BitTorrent, peers to upload the file in a swarm are selected by determining which peers upload the most to themselves. However, the number of peers to upload (i.e., number of unchoked peers) is fixed to four in its peer selection mechanism of BitTorrent, which yields inefficiency because the situation of the swarm may vary frequently (e.g., number of peers in the swarm, download rates, and upload rates). In this paper, we analyze the swarming system performance when the number of unchoked in BitTorrents is not static, but dynamic. For empirical investigation, we established a testbed in Seoul National University by modifying an open-source BitTorrent client, which is popular. Through our experiments, we show that an adaptive mechanism to adjust the number of unchoked peers considering the situation of the swarm is needed to improve the performance of BitTorrent.