• Natural Product Sciences
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• v.26 no.3
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• pp.230-235
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• 2020

A pregnane steroid, 3α-hydroxy-pregn-7-ene-6,20-dione (1), was isolated from a Hydractinia-associated Cladosporium sphaerospermum SW67 by repetitive column chromatographic separation and high-performance liquid chromatography (HPLC) purification. The planar structure of 1 was elucidated from the analysis of the spectroscopic data (1D and 2D NMR spectra) and LC-MS data. The absolute configuration of 1 was determined by interpretation of ROESY spectrum of 1, together with the comparison of reported spectroscopic values in previous studies. To the best of our knowledge, this is the first report of the identification of the pregnane scaffold from C. sphaerospermum, a natural source. Compound 1 was evaluated for its effects on lipid metabolism and adipogenesis during adipocyte maturation and showed that compound 1 substantially inhibited lipid accumulation compared to the control. Consistently, the expression of the adipocyte marker gene (Adipsin) was reduced upon incubation with 1. Further, we evaluated the effects of 1 on lipid metabolism by measuring the transcription of lipolytic and lipogenic genes. The expression of the lipolytic gene ATGL was significantly elevated upon exposure to 1 during adipogenesis, whereas the expression of lipogenic genes FASN and SREBP1 was significantly reduced upon treatment with 1. Thus, our findings provide experimental evidence that the steroid derived from Hydractinia-associated C. sphaerospermum SW67 is a potential therapeutic agent for obesity.

• Journal of Korean Society for Atmospheric Environment
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• v.16 no.1
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• pp.49-60
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• 2000

The concentration of atmospheric mercury(Hg0 and relevent environmental parameters that include both meteorological and criteria pollutant data were dtermined at hourly intervals during two field campaigns covering the periods of September 1997 and May/June 1998. The mean concentrations of Hg for the two study periods were computed as 3.94 and 3.43ngm-3, respectively. Through a separation of these data into both daytime and nighttime periods, we further analyzed diurnal variation patterns for Hg between two different seasons. Using our Hg data sets, we were able to recognize two contrasting diurnal variation patterns of Hg between two diffeerent seasons that can be characterized as: (1) the occurrences of peak Hg concentration during daytime(fall0 and (2) slight reductions in daytime Hg concentration relative to nighttiime Hg data(summer). To study the systematic differences in diurnal patterns between two different seasons, we analyzed Hg data in terms of different statistical approaches such as correlation(and linear regression0 and factor analysis. Results of these analyses consistently indicated that different mechanisms were responsible for controlling the daytime distribution patterns of Hg. The variation of boundary layer conditions betwen day/night periods may have been important in introducing the relative reduction in daytime Hg levels during summer. However, when the relationship between Hg and concurrently determined O3 is concerned, these differences are unlikely to be an effective sink mechanism within the ranges of ozone concentrations determined concurrently during this study, regardless of season. To further provide the general account for short-term variations in Hg distribution data, we should be able to describe the various factors underlying its sink mechanism.

• Journal of the Korea Organic Resources Recycling Association
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• v.20 no.2
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• pp.76-88
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• 2012

In this study, the treatment flow of the recyclables(paper, plastic, metal, etc.) collected and separated from MSW was investigated throughout entire phase from separation and collection to final disposal. Based on the site survey, most recyclables came into six material recovery facilities were comparatively managed well. However, some of recyclables showed irrelatively transportation flow. Because the main income source of the recyclables transportation companies is profit margin between treatment and transportation cost based on economics only therefore, it occurs an unnecessary route and long distance transportation while going to final destination. This unreasonable system should be a serious problem relating establishment of the resource recycling system. As a result of this study, in order to establish the reasonably resource recycling system, recycling center including material recovery facility has to manage the recyclables generated in close range preferentially. Also integrated its treatment system over a wide area will need to be considered as a basic step for the reasonable reasonably resource recycling system.

• Membrane Journal
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• v.26 no.1
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• pp.1-13
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• 2016

Fuel cells are regarded as a representative energy source expected to replace fossil fuels particularly used in internal combustion engines. One of the most important components is polymer electrolyte membranes (PEMs) acting as a proton conducting barrier to prevent fuel gas crossover. Since water channels act as proton pathways through PEMs, many researchers have been focused on the 'good phase-separation of hydrophilic moiety' which ensures high water retention under low humidity enough to keep the water channel for good proton conduction. Here, we summarized the strategies which have been adopted to synthesize sulfonated PEMs having high proton conductivities even under low humidified conditions, and hope this review will be helpful to design high performance hydrocarbon PEMs.

• Journal of Digital Convergence
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• v.12 no.7
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• pp.11-20
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• 2014

This paper finds that the ultimate source of problems facing Korean mobile telecommunications such as excessive subsidies of handsets, waste of resources, and a vicious circle of expensive handsets and high prices for services, is bundling of handset and subscription. The analysis suggests that bundling causes consumers to confuse about prices of handsets and services, and firms focus on handset subsidy competition rather than on upgrading service qualities and lowering prices due to anti-competitive effects of bundling. Because most of the cost of bundling is passed on to consumers, the welfare of consumers decline. In particular, equality among consumers worsens due to price discriminations. To resolve these problems, unbundling of handset and subscription is a necessity. Mixed bundling allowing separate selling of handsets under bundling does not seem to work as a cure because bundling tends to be a dominant strategy. The best regulatory policy is a complete separation of handset and subscription.

• Bulletin of the Korean Chemical Society
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• v.28 no.7
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• pp.1187-1194
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• 2007

The three major active isoflavonoids (calycosin-7-O-β -glucoside, isomucronulatol 7-O-β-glucoside, formononetin) and two main saponins (astragaloside I, astragaloside IV) in an extract of Radix Astragali were determined using rapid, sensitive, reliable HPLC/UV and LC-ESI-MS/MS methods. The separation conditions employed for HPLC/UV were optimized using a phenyl-hexyl column (4.6 × 150 mm, 5 μm) with the gradient elution of acetonitrile and water as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 230 nm. The specificity of the peaks was determined using a triple quadrupole tandem mass spectrometer equipped with an electrospray ionization (ESI) source that was operated in multiple reaction monitoring (MRM) in the positive mode. These methods were fully validated with respect to the linearity, accuracy, precision, recovery and robustness. The HPLC/UV method was applied successfully to the quantification of three major isoflavonoids in the extract of Radix Astragali. The results indicate that the established HPLC/UV and LC-ESI-MS/MS methods are suitable for the quantitative analysis and quality control of multi-components in Radix Astragali.

• The KIPS Transactions:PartB
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• v.11B no.3
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• pp.381-386
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• 2004

This paper proposes an independent component analysis(ICA) of the fixed-point(FP) algorithm based on Newton method by adding the kurtosis. The kurtosis is applied for clustering the components, and the FP algorithm of Newton method is applied for improving the analysis speed and performance. The proposed ICA has been applied to the problems for separating the 6-mixed signals of 500 samples and 8-mixed images of $512\times512$pixels, respectively. The experimental results show that the proposed ICA has always a fixed analysis sequence. The result can be solved the limit of conventional ICA which has a variable sequence depending on the running of algorithm. Especially, the proposed ICA can be used to classify and identify the signals or the images.

• Journal of the Korean earth science society
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• v.26 no.7
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• pp.698-705
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• 2005

Heavy rain fall in the Korean Peninsula often occurs in the summer season due to MCC (Mesoscale Convective Complex) with complex mechanism. We analysed the Characteristics and the developing mechanism of MCC occurred at 14 July 2004. The results are as follows: a) There is strong wind inflow from the South-west china sea with heavy moisture and this moisture flux acts as the source of heavy rain, b) Because of the separation of upper and lower atmosphere due to an inversion layer at 600hPa, atmosphere over the Korea Peninsula is suddenly unstable. c) This MCC shows strong shear not with wind direction, but with the wind speed, and this wind shear continues the thermodynamic unstability of the convective system. d) MCC was suddenly developed over Heuksando at 1400LST 14 July 2004. Thus we can say that the topography also was strongly associated with the development of MCC and it is also necessary to clarify the relationship between topography and MCC development. in future research.

• Journal of Electrochemical Science and Technology
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• v.8 no.3
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• pp.183-196
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• 2017

The research efforts directed at advancing water electrolysis technology continue to intensify together with the increasing interest in hydrogen as an alternative source of energy to fossil fuels. Among the various water electrolysis systems reported to date, systems employing a solid polymer electrolyte membrane are known to display both improved safety and efficiency as a result of enhanced separation of products: hydrogen and oxygen. Conducting water electrolysis in an alkaline medium lowers the system cost by allowing non-platinum group metals to be used as catalysts for the complex multi-electron transfer reactions involved in water electrolysis, namely the hydrogen and oxygen evolution reactions (HER and OER, respectively). We briefly review the anion exchange membranes (AEMs) and electrocatalysts developed and applied thus far in alkaline AEM water electrolysis (AEMWE) devices. Testing the developed components in AEMWE cells is a key step in maximizing the device performance since cell performance depends strongly on the structure of the electrodes containing the HER and OER catalysts and the polymer membrane under specific cell operating conditions. In this review, we discuss the properties of reported AEMs that have been used to fabricate membrane-electrode assemblies for AEMWE cells, including membranes based on polysulfone, poly(2,6-dimethyl-p-phylene) oxide, polybenzimidazole, and inorganic composite materials. The activities and stabilities of tertiary metal oxides, metal carbon composites, and ultra-low Pt-loading electrodes toward OER and HER in AEMWE cells are also described.

• The Journal of the Acoustical Society of Korea
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• v.18 no.8
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• pp.32-41
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• 1999

In this paper, an inversion method using chirp signals and two near field receivers is proposed. Inversion problems can be formulated into the probabilistic models composed of signals, a forward model and noise. Forward model to simulate chirp signals is chosen to be the source-wavelet-convolution planewave modeling method. The solution of the inversion problem is defined by a posteriori pdf. The wavelet matching technique, using weighted least-squares fitting, estimates the sediment sound-speed and thickness on which determination of the ranges for a priori uniform distribution is based. The genetic algorithm can be applied to a global optimization problem to find a maximum a posteriori solution for determined a priori search space. Here the object function is defined by an L₂norm of the difference between measured and modeled signals. The observed signals can be separated into a set of two signals reflected from the upper and lower boundaries of a sediment. The separation of signals and successive applications of the genetic algorithm optimization process reduce the search space, therefore improving the inversion results. Not only the marginal pdf but also the statistics are calculated by numerical evaluation of integrals using the samples selected during importance sampling process of the genetic algorithm. The examples applied here show that, for synthetic data with noise, it is possible to carry out an inversion for sedimentary layers using the proposed inversion method.