• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.3
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• pp.404-410
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• 2014

The effects of treatment with various complex carbohydrases such as Pectinex, Celluclast, Viscozyme, and Ultraflo on the physiochemical properties, polyphenol extraction yields and antioxidant activities of pear peel and pear core were investigated. When pear peel and pear core were treated with complex carbohydrases, the soluble solid content of peel increased, whereas it did not change significantly in the case of pear core. When pear peel and pear core were treated with Pectinex, significant improvement of soluble solid content was observed along with the highest extraction yield of reducing sugar content. Total sugar content increased in most of the enzyme treatment groups. In the case of pear peel, the Viscozyme treatment group showed the highest total polyphenol contents, total flavonoid contents, DPPH radical scavenging activity, ABTS radical scavenging activity, and SOD-like activity. When the flesh and core of pear were treated with Celluclast, total polyphenol contents increased. All enzyme treatment groups except for the Ultraflo treatment group showed increases in total flavonoid contents. With regard to pear flesh, the Celluclast group showed the highest DPPH radical scavenging activity. When pear core was treated with the four complex carbohydrases, DPPH radical scavenging activity and ABTS radical scavenging activity did not increase significantly. However, the SOD-like activity of all enzyme treatment groups significantly increased. Consequently, dry matter and soluble solid contents, polyphenol content, and antioxidant activity of pear peel and core could be improved by complex carbohydrase treatment.

• Current Research on Agriculture and Life Sciences
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• v.30 no.2
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• pp.68-75
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• 2012

This study was conducted to monitor the yields of useful substances extracted from Inonotus obliquus. Optimization of ultrasonic-assisted extraction process was carried out by using response surface methodology under different extraction conditions. A central composite design was applied to investigate the effects of independent variables such as extraction time ($X_1$), ethanol concentration ($X_2$) and extraction temperature ($X_3$) on dependent variables such as soluble solid yield ($Y_1$), total phenol contents ($Y_2$), total flavonoid contents ($Y_3$) and browning color($Y_4$). Soluble solid yield was affected by ethanol concentration and extraction temperature. The maximum soluble solid yield was 18.02% at 20.47 min ($X_1$), 42.85% ($X_2$) and $69.57^{\circ}C$ ($X_3$) in saddle point. Total phenol contents were highly affected by ethanol concentration and extraction temperature. The maximum total phenol contents were 71.57mg GAE/g at 21.60min ($X_1$), 45.19% ($X_2$), $69.68^{\circ}C$ ($X_3$). The electron donating ability was affected by extraction temperature and extraction time. Total flavonoid contents were affected by only extraction temperature. The maximum total flavonoid contents were 35.98 mg RE/g at 22.53min ($X_1$), 46.37% ($X_2$), $69.56^{\circ}C$ ($X_3$) in saddle point. The browning color was highly affected by extraction time, ethanol concentration and extraction temperature. The maximum browning color was at 22.00 min ($X_1$), 46.89% ($X_2$), $69.71^{\circ}C$ ($X_3$) in saddle point. As a result, the optimum extraction conditions were predicted; extraction time of 21.50 min, ethanol concentration of 44.87% and extraction temperature of $69.635^{\circ}C$.

• Journal of Bio-Environment Control
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• v.25 no.2
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• pp.111-117
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• 2016

The objective of this study was to analyze the microclimate and the growth of tomato and lettuce in the greenhouses covered with spectrum conversion films using different phosphor particles sizes. Two spectrum conversion films using phosphor particles larger than $10{\mu}m$ (Micro-film) and smaller than 500 nm (Nano-film) in radius, and poly-ethylene (PE) film were used in double-layered greenhouses as outer coverings. PE films were used as inner coverings in all the greenhouses. Thickness of the films for inner and outer coverings was 0.06 mm. Tensile strength, elongation, and tearing resistance of the Micro- and Nano-films were not different from those of the PE film. Transmittances at a wavelength of 300-1100 nm were a little higher at the Micro-film and lower at the Nano-film than that of the PE film, respectively. Air temperatures at the Micro- and Nano-films were over $2^{\circ}C$ higher than at the PE film, but no significant difference was observed between the two light conversion films. The soil temperature at the Nano-film was $1.5^{\circ}C$ and $3^{\circ}C$ higher than at the Micro- and PE films, respectively. The yields of tomato at the Micro- and Nano-films were 12% and 14% higher than at the PE film, but no significant difference was observed between the two spectrum conversion films. The total soluble solid showed no significant differences among all the films. The yields of lettuces at the Micro- and Nano-films were 27% and 59% higher than at the PE film. Hunter's red (a) value of the lettuce leaf was the highest at the Nano-film. In this experiment, tomatoes requiring high irradiation were better at the Nano film, while lettuce requiring low irradiation better at the Micro film.

• 한국신재생에너지학회:학술대회논문집
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• 2010.11a
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• pp.101-101
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• 2010

Anaerobic digestion(AD) is the most promising method for treating and recycling of different organic wastes, such as organic fraction of municipal solid waste, household wastes, animal manure, agro-industrial wastes, industrial organic wastes and sewage sludge. During AD, i.e. organic materials are decomposed by anaerobic forming bacteria and fina1ly converted to excellent fertilizer and biogas which is a mixture of carbon dioxide and methane. AD has been one of the leading technologies that can make a large contribution to produce renewable energy and to reduce $CO_2$ and other green-house gas(GHG) emission, it is becoming a key method for both waste treatment and recovery of a renewable fuel and other valuable co-products. Currently some 80% of the world's overall energy supply of about 400 EJ per year in derived from fossil fuels. Nevertheless roughly 10~15% of this demand is covered by biomass resources, making biomass by far the most important renewable energy source used to date. The representative biofuels produced from the biomass are bioethanol, biodiesel and biogas, and currently biogas plays a smaller than other biofuels but steadily growing role. Traditionally anaerobic digestion applied for different biowaste e.g. sewage sludge, manure, other organic wastes treatment and stabilization, biogas has become a well established energy resource. However, the biowaste are fairly limited in respect to the production and utilization as renewable source, but the plant biomass, the so called "energy crops" are used for more biogas production in EU countries and the investigation on the biomethane potential of different crops and plant materials have been carried out. In Korea, with steadily increasing oil prices and improved environmental regulations, since 2005 anaerobic digestion was again stimulated, especially on the biogasification of different biowastes and agro-industrial biomass including "energy crops". This study have been carried out to investigate anaerobic biodegradability by the biochemical methane potential(BMP) test of animal manures, different forage crops i.e. "energy crops", plant and industrial organic wastes in the condition of thermophilic temperature, The biodegradability of animal manure were 63.2% and 58.2% with $315m^3CH_4/tonVS$ of cattle slurry and $370m^3CH_4/tonVS$ of pig slurry in ultimate methane yields. Those of winter forage crops were the range 75% to 87% with ultimate methane yield of $378m^3CH_4/tonVS$ to $450m^3CH_4/tonVS$ and those of summer forage crops were the range 81% to 85% with ultimate methane yield of $392m^3CH_4/tonVS$ to $415m^3CH_4/tonVS$. The forge crops as "energy crops" could be used as good renewable energy source to increase methane production and to improve biodegradability in co-digestion with animal manure or only energy crop digestion.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.3
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• pp.188-193
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• 2004

Fifty-day-old plug seedlings of yacon (Polymnia sonchiflolia Poeppig ＆ Endlicher) were transplanted on Mar. 23, Apr,8, Apr,23, May 8, May 23, and June 7,2002 at Jeju to evaluate the effect of transplanting date on the growth and yield of yacon in Jeju island. Delaying the transplanting until May 23 greatly decreased plant height stem height, main stem diameter and number of nodes per plant. The number of marketable tuberous roots was about 6.0 per plant with the early transplanting from March 23 to April 231 but decreased to 1.9 per plant with the latest transplanting time, June 7. The yield of marketable tuberous roots of early transplanted (March 23 to April 23) yacon was about 27 MT/ha, and was 32.4 MT/ha at April 23 transplanting, but was remarkably reduced at the three later transplantings, May 8, May 23, and June 7. Dry matter and soluble solid contents of marketable tuberous roots were 14％ and 15％, respectively, regardless of transplanting date. The weight of a marketable tuberous root ranged from 153 to 183.9 g at the first three transplantings, but gyeatly reduced with delaying transplanting until May 7. The optimum transplanting date for yacon in Jeju island seems to be from mid-April to late April considering tuberous root yields, the risk of late-spring frost, and cost of seedling raising.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.4
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• pp.680-686
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• 1996

Investigations were conducted on the effects of different blanching solutions and time on tile quality of carrot juice to minimize undesirable changes in color, taste and flavor of raw carrot. Juice yields were 65.9% in raw juice(RJ), 51.7~55.3% in juice by blanching in water(JBIW) and 44.6% in juice by blanching in acetic acid solution(JBIA). pH of JBIW was 6.12~6.37, similar to RJ, while that of JBIA was 4.85. Soluble solid was in the decreasing order of RJ, JBIW and JBIA. Turbidities of JBIW for 1 min and JBIA for 5 min after 3 day storage at $4^{\circ}C$ were maintained by 94% compared to the original juice. Only 44~46% of $\beta-carotene$ was extracted from carrots during pressing, and f-carotene was extracted to a greater extent than $\alpha-carotene.$ Sensory evaluation indicated that JBIW for 1 min was superior to nth and commercially available juice in overall acceptance.

• Applied Chemistry for Engineering
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• v.9 no.2
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• pp.165-171
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• 1998

The $Y_1Ba_2Cu_3O_{7-x}$(123) superconductor powders were prepared by pyrophoric synthesis method(PSM) using $Y_2O_3$(99.9%), $BaCO_3$(99.9%), and CuO(99.9%) powders. The phase formation and reaction kinetics of 123 superconductor manufactured with powders prepared in various pHs of pyrophoric synthetic solution have been studied through the experiments at various heat treatment temperatures and times. Inductively coupled plasma(ICP) spectroscopy and scanning electron microscopy(SEM) measurements were performed to examine the composition and morphology of the sample. X-ray diffraction(XRD) analysis was done to determine phase formation and conversion ratio of Y-Ba-Cu-O systems. The 123 powder prepared at pH 7(${\pm}0.3$) yields the best result in terms of purity, homogeneity, and reactivity. The activation energies(${\Delta}E_a$) of 123 phase formation were found to be 191 kJ/mol and 230kJ/mol in solid state reaction method and pyrophoric synthesis method, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.2
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• pp.204-213
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• 2008

Four ruminally cannulated Holstein cows averaging 43 days in milk (DIM) were used in a $4{\times}4$ Latin square to determine the effect of feeding extruded soybean, ground canola seed and whole cottonseed on ruminal fermentation and milk fatty acid profile. One hundred and twenty lactating Holstein cows, 58 (${\pm}31$) DIM, were assigned to four treatments in a completely randomized block design to study the effects of the three types of oilseeds on production parameters and milk fatty acid profile. The four diets were a control diet (CON) and three diets in which 10% extruded soybean (ESB), 5% ground canola seed (GCS) and 10% whole cottonseed (WCS) were included, respectively. Diets consisted of concentrate mix, corn silage and Chinese wild rye and were balanced to similar concentrations of CP, NDF and ADF. Ruminal fermentation results showed that ruminal fermentation parameters, dry matter intake and milk yield were not significantly affected by treatments. However, compared with the control, feeding cows with the three oilseed diets reduced C14:0 and C16:0 and elevated C18:0 and C18:1 concentrations in milk, and feeding ESB increased C18:2 and cis9, trans11 conjugated linoleic acid (CLA). Production results showed that feeding ESB tended to increase actual milk yield (30.85 kg/d vs. 29.29 kg/d) and significantly decreased milk fat percentage (3.53% vs. 4.06%) compared with CON. Milk protein (3.41%) and solid non-fat (13.27%) from cows fed WCS were significantly higher than from cows fed CON (3.24% and 12.63%, respectively). Milk urea N concentrations from cows fed the ESB (164.12 mg/L) and GCS (169.91 mg/L) were higher than cows fed CON (132.31 mg/L). However, intake of DM, 4% fat corrected milk, energy corrected milk, milk fat and protein yields, milk lactose percentage and yield, somatic cell count and body condition score were not affected by different treatments. The proportion of medium-chain fatty acid with 14 to 16 C units in milk was greatly decreased in cows fed ESB, GCS and WCS. Feeding ESB increased the concentration in milk of C18:1, C18:2, C18:3 and cis9, trans11-CLA content by 16.67%, 37.36%, 95.24%, 72.22%, respectively, feeding GCS improved C18:0 and C18:1 by 17.41% and 33.28%, respectively, and feeding WCS increased C18:0 by 31.01% compared with feeding CON. Both ruminal fermentation and production trial results indicated that supplementation of extruded soybean, ground canola seed and whole cottonseed could elevate the desirable poly- and monounsaturated fatty acid and decrease the medium chain fatty acid and saturated fatty acid content of milk fat without negative effects on ruminal fermentation and lactation performance.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.27 no.4
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• pp.373-379
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• 1994

Final biomass yields(cells/ml) and growth rates(divisions/day) of 4 clones of marine diatoms isolated from the Korean coastal waters were measured in media with 6 different levels of added cadmium concentrations. A neritic diatom, GS-12(Chaetoceros sp.), showed no growth at 0.1ppm cadmium, and its $IC_{50}$ for final biomass yield and growth rate was 0.03 and 0.02ppm, respectively. Two clones isolated from tidal pool, NC-37 and NC-29, showed enhanced tolerance to cadmium toxicity. Extremely high tolerance to cadmium addition was found in J-21 from a eutrophicated bay, with its high $IC_{50}$ for biomass yield(1.07ppm) and growth rate(1.92ppm). Present results implied a habitat related pattern of coastal diatom clones in the cadmium tolerances. Except GS-12, the other three diatom clones are considered to be highly tolerant to cadmium stresses.

• The Korean Journal of Mycology
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• v.22 no.2
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• pp.130-137
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• 1994

This experiment was carried out to investigate proper conditions for protoplast isolation and regeneration from mycelia of Lyophyllum decastes. Novozym 234(10 mg/ml) with 0.6 M $MgSO_4$ in phosphate buffer(pH 4.0) was proper for protoplast isolation. The optimal reaction time of the mycelium with the lytic enzyme was four hours in shaking condition at 120 strokes per min. When the mycelium of L. decastes was cultured at $24^{\circ}C$ for 5 days, the formation of protoplasts was effective. The liquid medium was more effective for protoplast isolation than the solid medium. In the liquid medium, high yields of protoplasts were obtained from 0.6 M $MgSO_4$ osmotic stabilizer. Protoplasts of L. decastes were regenerated to normal hyphal growth and the regeneration frequency of the protoplasts in the complete agar medium containing Triton X-100(0.0025%) was $5.94{\sim}8.32%$. The regeneration medium stabilized with 0.6 M sucrose was the best for regeneration of the protoplasts. In contrast to protoplast formation, regeneration was inhibited by the inorganic salts used as osmotic stabilizer.