• Food Science and Preservation
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• v.21 no.6
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• pp.917-924
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• 2014

Normal- and late-harvested Fuji apples were fermented using the rapid-fermenting yeast strain Saccharomyces cerevisiae SS89. The late-harvest apples showed a slightly higher soluble-solid content with a lower level of total-acid and moisture (p<0.05) contents as well as hardness (p<0.05) than the normal-harvest apples. During the fermentation, the apples had similar changes in the pH and total-acid content regardless of the harvest time, but the increases in the alcohol content and yeast viable count with the decrease of the soluble-solid content were more rapid in the late-harvest apples than in the normal-harvest apples. After the completion of the fermentation, the soluble-solid and alcohol contents became very similar. The late-harvest cider showed a high total phenolic-compound content and a high DPPH radical scavenging effect, although these were slightly lower than those of the normal-harvest cider. It also showed a higher malic-acid content and higher hue color (p<0.05), Hunter's L, and b (p<0.05) values than the normal-harvest cider. In the sensory evaluation, the late-harvest cider obtained a higher score in taste and a lower score in color compared to the normal-harvest cider.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.367-370
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• 2003

This study was carried out to investigate the possibility for reuse of solid organic wastes such as saccharified newspapers and boxes by two-phase anaerobic fermentation system. When 15g of newspaper and box wastes were digested for 24 days by batch fermentation, tCOD removal rate were found to be 60.9 and 62.4%, respectively. During this period, the amounts of biogas produced were 6.95 and 6.43L. The removal efficiencies of total solid were 34.8 and 33.4%, and those of volatile solid were 40.0 and 39.2%, respectively. That pH was around 7.5 after 20-days operation means methane fermentation is well advanced. In case of semicontinuous reaction, tCOD removal efficiencies of newspaper and box wastes were 64.7 and 65.0%, respectively for 14-days operation. It has been shown that each of the average biogas amounts produced after 25 days operation (stabilization stage for methane fermentation) was 0.31 and 0.30L/g dry wt./day, respectively, and each methane contents was 57.3 and 56.2%, respectively. After the reaction continued for 25 days, pHs in the anaerobic acidogenic and methanogenic fermenters were shown to be 5.0 and 7.5, respectively.

• Korean Journal of Food Science and Technology
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• v.24 no.1
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• pp.86-91
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• 1992

To develop an effective utilization method of skipjack tuna viscera, fish meal by an improved fermentation method(FFMA) was prepared by fermentation of the solid materials separated from autoclaved viscera with Aspergillus oryzae for 72 hours and by adding the concentrated soluble extracts separated from autoclaved viscera to the fermenting solid materials during fermentation, and FFMA fish meal was compared with the fish meals prepared by Kato mathod(FFMN) and conventional nonfermenting method(NFM). FFMN fish meal was prepared by fermenting the solid materials separated from autoclaved viscera with Aspergillus oryzae for 17 hours without adding the soluble extract. The extracts from FFMA fish meal(FFMA-E) and raw viscera(RM-E) were also prepared respectively after digestion with proteases obtained from Bacillus subtilis and Aspergillus oryzae and compared with each other on the contents of free amino acids. The peroxide values decreased greatly in contents of $Vitamin\;B_{1},\;B_{2}\;and\;C$ significantly increased in FFMA fish meal compared with those of other fish meals. The total free amino acid content of FFMA-E was significantly higher then that of RM-E. The total free essential amino acid content also greatly increased in FFMA-E in which threonine, methionine and lysine showed remarkable increments. Almost all individual nonessential amino acids were higher in FFMA-E then in RM-E. The content of taurine, a nonprotein amino acid, greatly increased compared with other nonprotein amino acids in both extracts.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.529-532
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• 2001

Korean food waste, one of the abundantly available but environmentally problematic organic wastes in Korea, was utilized as solid-substrate by fungal strain Aspergillus niger ATcC 6275 for the production of enzymemixture containing amylase, cellulase and xylanase. The enzyme mixture can be used as high value-added animal feed. Solid-state fermentation method yielded a 84-fold enhancement in xylanase activity compared with submerged fermentation method. The effect of incubation period, incubation temperature, pH of medium, moisture content, inoculum size and enrichment of the medium with nitrogen and carbon sources were observed for optimal production of these enzymes The optimal amylase activity of 33.10 U/g, cellulase activity of 24.41 U/g, xylanase activity of 328.84 U/g were obtained at 8 days incubation with 50%(w/w) soy bean flake, with incubation temperature of $25^{\circ}C$, pH of 6.38, optimal moisture content of 55% and with inoculum size of $3.8{\times}10^6$spore/g. Enzyme activities were enhanced when ImM $CaSO_4$, 2% Malt extract and 2% galactose were added as mineral, nitrogen and carbon enrichment respectively.

• Food Science and Preservation
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• v.17 no.1
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• pp.165-168
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• 2010

It is known that temperature during solid fermentation using rice bran is increased upto 60-70 degree. To investigate the major microflora regarding temperature maintenance of rice bran bathing, we first isolated predominant microbes using various media by a limiting dilution method. The RNA of isolated strains were purified and sequenced. The rRNA sequencing revealed that the selected strains were similar to Bacillus coagulans according to their taxonomical relationships. Together, these results indicate that Bacillus coagulans is a major kind of microflora during solid fermentation using rice bran.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2011.11a
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• pp.33-33
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• 2011

Artificial silk that called "Ponggi (Gyeongsangbukdo, Korea) In-Gyeun" in Korea is entirely viscose rayon of which made main component refined larches and cotton linter. It also is natural fabrics with light weight, cool texture, free from the body and well ventilation property, so, feel good touch during the wearing. In addition, it often used as summer cloth and bedding because it has good absorbancy to perspiration and antistatic. The "Hanji", made of paper mulberry, is known as useful material for human. In this research focused on dyeability of Artificial silk/paper mulberry mixed fabrics using Dendropanax morbifera Lev.(called as "Hwangchil") Especially, the results were in comparison to the dyeing properties of Hwangchil with liquid/solid fermentation or not. As the results, The ${\Delta}E$ difference was dominated when the fabric dyed with fermentation by solid state at $60^{\circ}C$. And it was confirmed that the pH of fermented dye had an important influence in the coloration.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.6
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• pp.501-505
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• 1991

To evaluate the suitability of solid-culm crops for silage, culm characteristics and the variation of solidness in several solid-culm and hollow-culm cultivars of wheat and triticale were examined both in the field and greenhouse. The degree of culm solidness and the voiume of air space in each internodes were measured at heading. The solidness of solid-culm were increased by the development of pith connected with vascular bundles. There was a variation in solidness among the crops tested. The degree of solidness in hollow -culm crops was 14.6% and 15.0% for 'Wonkwang' wheat and 'Shinkihomil' triticale, whereas in solid-culm crops the degree was 77.0-100% for durum wheats, 61. 0-95.8% for bread wheats and 18,7-57,0% for triticales. For 'Golden ball' which had the most solid culms among durum wheats tested higher solidness was observed in the greenhouse than the field, in the high internodes than low ones and in tiller culms than main stems. The solid-culm crops had a smaller air volume in internodes (1.01-2.30ml) than did 'Shinkihomil' (4.85ml) and this characteristics was considered to be advantageous for air removal and lactic fermentation at ensilaging.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.9
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• pp.1380-1387
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• 2014

The purpose of this study was to develop a mouthwash product with solid fermented oriental medicinal herb (OMH). Solid fermentation of magnolia, liquorice, and cnidium by Phellinus linteus mycelium was carried out successfully when 30% water was added to the medium, whereas 10% brown rice powder was required as an extra nutrient for solid fermentation of mint besides water. The amount of total phenol compounds and DPPH radical scavenging activity of OMH increased significantly (P<0.05) upon solid fermentation. Anti-microbial activities of fermented OMH also increased and were approximately 100-fold greater than those of unfermented samples. Oral pathogens such as Staphylococcus epidermis, Streptococcus pyogenes, Candida albicans, or Streptococcus mutans were used for determination of anti-microbial effects of OMH. Formulation of the mouthwash was developed based on the results of the sensory evaluation. Among seven formulas, the best formula chosen by the sensory evaluation was as follows: mouthwash prepared with 0.075% ethanol extract of solid fermented OMH as a main ingredient, 83.64% hot water extract of mint and clove (100:15, v/v) as a mouthwash base component, and other miscellaneous ingredients, including sodium fluoride, menthol, and surfactants. Data from a consumer's preference test with 30 participants, overall acceptance, and willingness to buy the product developed in this study were all significantly higher for the tested mouthwash compared to mouthwash on the market manufactured with OMH but with a different formula. Duration of freshness of the mouthwash after usage as determined by Breath Checker was not significantly different between the two samples, although the duration of our product was slightly longer than that of the commercial product mentioned above.

• Food Science and Biotechnology
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• v.18 no.3
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• pp.700-705
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• 2009

In this study, the volatile compounds in 9 commercial fermented soybean pastes were extracted and analyzed by headspace-solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS), respectively. A total of 63 volatile components, including 21 esters, 7 alcohols, 7 acids, 8 pyrazines, 5 volatile phenols, 3 ketones, 6 aldehydes, and 6 miscellaneous compounds, were identified. Esters, acids, and pyrazines were the largest groups among the quantified volatiles. About 50% of the total quantified volatile material was contributed by 5 compounds in 9 soybean paste samples; ethyl hexadecanoate, acetic acid, butanoic acid, 2/3-methyl butanoic acid, and tetramethyl-pyrazine. Three samples (CJW, SIN, and HAE) made by Aspergillus oryzae inoculation showed similar volatile patterns as shown in principal component analyses to GC-MS data sets, which showed higher levels in ethyl esters and 2-methoxy-4-vinylphenol. Traditional fermented soybean pastes showed overall higher levels in pyrazines and acids contents.

• Herbal Formula Science
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• v.29 no.4
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• pp.239-252
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• 2021

Objectives : This study is to effect of improving muscle atrophy through water extract on the solid-phase fermentation extraction with Phellinus linteus of discarded Schisandra chinensis in an atorvastatin-induced atrophy C2C12 cell. Methods : C2C12 myoblast were differentiated into myotube by 2% horse serum medium for 6 days, and then treated solid-phase fermentation(S-P) extract at different concentrations for 24h. To investigate the effect of S-P extract on the induction of muscle atrophy and expression of atrophy-related genes and apoptosis in differentiated C2C12 myotubes using a GSH, ROS, real-time PCR, western blots analysis. Results : As a result of treatment with atorvastatin at concentrations of 5, 10, and 20 uM on the 6th day of differentiation in C2C12 myotube cells, it was confirmed that the cell morphology was damaged in a concentration-dependent manner, and the length and thickness of the myotube also decreased in a concentration-dependent manner. Treatment with S-P extract (50, 100 and 200 ㎍/㎖) increased of GSH and inhibited ROS in the atorvastatin-induced muscle atrophy cell model at a concentration that did not induce toxicity. In addition, it was confirmed that it has an effect on muscle reduction by inhibiting apoptosis of muscle cells as well as being involved in protein production and degradation of muscle cells. Conclusions : Atorvastatin-induced atrophy C2C12 cell, S-P extract activates related to differentiation/generation and proteolysis, and inhibits cell death of atrophy in C2C12 cell. Based on this, it is necessary to prove its effectiveness through animal models and human application test, but it is considered to be discarded Schisandra chinensis can present the potential for development as a recycling industrial material.