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Development of Functional Food Materials from Acanthopanax senticosus-Fermented Mushroom Mycelia (가시오가피 버섯균사체 발효물의 기능성 식품 소재 개발)

  • Cho, Ju-Hyun;Choi, Goo-Hee;Park, In-Jae;Baik, Soon-Ok;Kim, Hyung-Ha;Kim, Choong-Sik
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.3
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    • pp.411-418
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    • 2014
  • Three mushroom mycelia, Ganoderma lucidum, Hericium erinaceum, and Phellinus linteus, were separately diluted with the natural culture media Acanthopanax senticosus. Solid-state fermentation was used to produce three different A. senticosus-fermented mushroom mycelium groups: G. lucidum mycelia, H. erinaceum mycelia, and P. linteus mycelia. The resulting mycelia were analyzed to assess their efficacies as health functional foods. Optimized fermentation conditions were determined by considering the density and growth speed of mycelia in each A. senticosus-fermented mushroom mycelium group. The cultured mushroom mycelia under the optimized conditions were extracted using water and 70% ethanol. Extraction was followed by filtration, concentration and freeze-drying to produce extract powder of A. senticosus-fermented mushroom mycelia: Water extracts (FM-5111, FM-5121, and FM-5131) and 70% ethanol extracts (FM-5112, FM-5122, and FM-5132). Analysis of extract powder of A. senticosus-fermented mushroom mycelia was performed using the maker compounds eleutheroside B and eleutheroside E. Analysis of ${\beta}$-glucan contents was performed by enzymatic procedures.

Distribution of Trypsin Indigestible Substrate (TI) in Seafoods and Its Changes during Processing 2. Changes in TI and In Vitro Apparent Digestibility of Boiled and Dried Anchovy during Processing and Storage (어패류의 Trypsin활성 저해물질의 분포와 가공중의 변화 2. 자건멸치 가공저장중의 Trypsin활성 저해물질과 In Vitro Apparent Digestibility의 변화)

  • LEE Kang-Ho;JO Jin-Ho;RYU Hong-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.17 no.2
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    • pp.101-108
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    • 1984
  • In order to study the optimal conditions of processing and storage for boiled and dried anchovy (Engraulis japonica) with high protein digestibility, the contents of trypsin indigestible substrate (TI) and in vitro apparent protein digestibility were determined. Peroxide value (PoV), TBA number and nonenzymatic brown pigments, that accounted for important antinutritional factors, were also measured and confirmed the relationship between those factors and formation of TI or in vitro protein apparent digestibility. The results were as follows; Samples boiled for 5 minutes showed the lower content of TI than the other samples boiled for 0.5 min. or 1 min. Hot air dried products had a lower TI content in comparison with the other dried ones such as sun dried or freeze dried products. It was revealed that the lower temperature ($8{\pm}1^{\circ}C$) did not affect to a great degree of forming TI and falling in vitro digestibility comparing to high temperature ($26{\pm}1^{\circ}C$) during storage. The lowest TI content (0.173 mg/g solid) was noted in the samples for 5 minutes and then sun drying after 56 days storage at $9{\pm}1^{\circ}C$. A rapid decrease of in vitro protein digestibility occurred within 0.5 min. of boiling and showed the value $85.3\%$. Freeze dried samples possessed the highest in vitro protein digestibility ($85.9\%$), when compared to sun dried or hot air dried products. Fat oxidation and nonenzymatic browning were proceeded with the various boiling times, drying methods and storing temperatures. It was noted that boiling for 5 minutes and freeze drying accelerate the fat oxidation significantly. More nonenzymatic brown pigments was developed in samples boiled for shorter time (0.5 min.) and that stored at high temperature ($26{\pm}1^{\circ}C$) than the other products. Therefore, fat oxidation and nonenzymatic browning assumed to be a major inhibitory reaction in enzyme digestion and those might be an important role in forming TI in boiled and dried anchovy products during processing and storage.

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Study of CO2 Carbonation-Regeneration Characteristics of Potassium-Based Dry Sorbents According to Water Vapor Contents of Inlet Gas and Regeneration Temperature in the Cycle Experiments of Bubbling Fluidized-Bed Reactor (회분식 기포유동층 반응기에서 K-계열 건식흡수제의 주입수분농도 및 재생반응온도에 따른 CO2 흡수-재생 반응특성 연구)

  • Park, Keun-Woo;Park, Yeong Seong;Park, Young Cheol;Jo, Sung-Ho;Yi, Chang-Keun
    • Korean Chemical Engineering Research
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    • v.47 no.3
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    • pp.349-354
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    • 2009
  • In this study, a bubbling fluidized-bed reactor was used to study $CO_2$ capture from flue gas using a potassium-based dry sorbent. A dry sorbent, manufactured by the Korea Electric Power Research Institute, consists of 35% of $K_2CO_3$ for $CO_2$ absorption and 65% of supporters for mechanical strength. $H_2O$, a reactant of the carbonation reaction, was supplied in the reactor as a form of saturated water vapor at a given temperature. The experiment of the regeneration reaction was performed by raising up to a given temperature using $N_2$ as a fluidization gas. It was indicated that sorption capacity and regenerability of dry sorbents showed high-efficiency at $1.97\;mol\;H_2O/mol\;CO_2$ and $400^{\circ}C$, respectively. The regenerated sorbent samples were analyzed by TGA to confirm the extent of the reaction. When the regeneration temperature was $150^{\circ}C$, the regenerability of dry sorbents was about 60%, which was capable of applying those sorbents to a two-interconnected fluidized-bed reactor system with continuous solid circulation. The results obtained in this study can be used as basic data for designing and operating a large scale $CO_2$ capture process with two fluidized-bed reactors.

The Quality Characteristics of Wheat-Makgeolli Made from Different Cultivars and Milling Rates (국산 밀 품종 및 제분율에 따른 막걸리의 품질 특성)

  • Sim, Eun-Yeong;Lee, Seuk Ki;Woo, Koan Sik;Kim, Hyun-Joo;Kang, Chon-Sik;Kim, Si Ju;Oh, Sea-Kwan;Park, Hye-Young
    • The Korean Journal of Food And Nutrition
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    • v.29 no.5
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    • pp.777-784
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    • 2016
  • This study aimed to evaluate the quality characteristics of wheat-Makgeolli (WM), a traditional Korean cereal alcoholic drink, using three varieties of wheat, namely Jokyoung (JK), Baegjoong (BJ) and Keumkang (KK). Samples of WM brewed from 100%, 85% and 70% milling rates of the three Korean wheat cultivars were analyzed for alcohol, pH, coloring degree, total acids, soluble solid, free sugars, and organic acids. As the milling rates in wheat decreased, total sugar content in WM increased while the pH of all samples decreased. The WM exhibited 0.95~1.27% in acidity, $10.2{\sim}12.5^{\circ}Brix$ in total sugar, and 14~16% in alcohol content. The most organic acids in WM was lactic acid, ranging in all the samples from 85.3~650.3 mg%. The results showed that BJ under a 70% milling rate had the highest reducing sugar contents and 15.97% in alcohol content. The carbohydrate content increased with the milling rate of wheat. Resulting in a positive correlation between carbohydrate content of wheat and total acids, reducing sugars (p<0.001), and alcohol content (p<0.05) in WM. Total sugar content is positively correlated with alcohol and reducing sugar content (p<0.001). Considering the yield, the milling rates will be adjusted to raw material prices.

Changes in Quality of Citron Juice by Storage and Extraction Conditions (유자과즙의 저장 및 착즙조건에 따른 품질변화)

  • Park, Kee-Jai;Jung, Sung-Won;Kim, Jong-Hoon;Jeong, Jin-Woong
    • Applied Biological Chemistry
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    • v.38 no.2
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    • pp.141-146
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    • 1995
  • Changes of physicochemical properties of citron juice prepared by two different extraction methods, rotary-crushing and belt-pressing method, were investigated during the storage at $5^{\circ}C$ and $-20^{\circ}C$. Temperature drop of citron juice extracted by belt-pressing method was faster than that of citron juice prepared by rotary-crushing method and its freezing point was $0.8{\sim}0.9^{\circ}C$. During the storage, pH of stored citron juice with rotary-crushing method was increased up to 3.5 after 6 months storage while that of citron juice extracted by belt-pressing method was not changed significantly during the same storage time. Acidity of rotary-crushed citron juice was reduced a little more than that of belt-pressed citron juice during the storage. However, changes of soluble solid content were influenced largely by the storage temperature than by the extraction method. Contents of formol nitrogen and vitamin C were reduced remarkably in all of stored citron juice and $92{\sim}82%$ of farmol nitrogen and $72{\sim}43%$ of vitamin C were remained after 6 months of storage. Among the changes of color value, L values were reduced in the whole stored citron juice and a and b value had a different change pattern respectively according to the extraction and storage temperature. Changes in the content of both amino acid and fatty acid compositions was also observed after same storage period. Especially, in the case of change of fatty acid composition, content of linoleic acid and linolenic acid were reduced after 6 months storage, while those of palmitic acid, stearic acid and oleic acid were increased.

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A Study on the Forest Vegetation and Soil-environmental Factors Affecting the Water Quality of Iwonch on Stream (이원천 수질에 미치는 삼림식생과 토양환경요인)

  • Bang, Je-Yong;Yang, Keum-Chul
    • Korean Journal of Environmental Biology
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    • v.27 no.2
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    • pp.183-190
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    • 2009
  • Characterization of the analysis of forest vegetation, soil environmental conditions and water quality were performed from March 2003 to March 2007. The two basins were characterized by cultivated area (Kaesim reservoir) and mountain area (Jangchan reservoir), and divided into eleven small basins, where dynamics of pollutants, forest vegetation and soil environmental conditions were surveyed. The vegetation can be divided into 10 types by $Z\ddot{u}rich$-Montpellier school's method. Pearson coefficients between vegetation type and water quality were correlated with dissolved oxygen (DO) in the Quercus variabilis community at the 5% level and total phosphorus (TP) in the Larix leptolepis plantation at the 1% level. Especially total phosphorous and total nitrogen increased in small basins where the proportion of cultivated and residential area increased. The analysis of influences of pollutant discharge on water quality showed that pollutant charge was very low in forest land area ($Y_{T-P}$=-0.0017X+0.2215, r=0.16, $Y_{COD}$=- 0.0395X+8.5051 r=0.47). The soil types of western area were comparatively simple, but those of eastern area were complicated with regosols, red-yellow soils, lithosoles, etc. The pH, total solid (TS) and volatile substance (VS) of the forest and agricultural land soils collected in each site were 5.4~6.9, 75.8~80.2%, and 3.80%~5.80%, respectively. According to the analytical result of soil environmental conditions, heavy metal contents fell short to the mean value of natural conditions. Runoff amount (Y) and depth of topsoil (X) were negatively correlated, $Y_{ron}=-1.0088X_{top}+35.378$ (r=0.68). The correlation was much lower in up-stream but much higher in down-stream, because permeation into soil particle was larger on down-stream due to its more or less gentle slope. Pearson coefficients between soil pH and water pH were statistically significant at 1% level.

Biogas Production from Anaerobic Co-digestion Using the Swine Manure and Organic Byproduct (돈분과 유기성 부산물을 혼합한 혐기소화에서 바이오가스 생산)

  • Kim, W.G.;Oh, I.H.;Yang, S.Y.;Lee, K.M.;Lee, S.I.
    • Journal of Animal Environmental Science
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    • v.17 no.1
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    • pp.49-54
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    • 2011
  • Animal manure is produced annually 43.7 million tonnes in Korea. Among them, about 85.6 % are used as compost or liquid fertilizer to the agricultural land. The animal manure can be effectively utilized by mixing with organic byproducts that result in generation of biogas from anaerobic co-digestion process. This study aimed to optimize the content of total solid materials (TS) and determine the effect of organic byproduct on the co-digestion process. Prior to the byproduct treatments, determination of proper content of TS was conducted by controlling at 5 or 10 %. For the byproduct treatments, swine manure without adding the byproduct was used for control treatment, and swine manure mixed with either corn silage or kitchen waste was used for other treatments. Volume of biomethane ($CH_4$) generated from digested materials was quantified before and after byproduct treatments. In result, a 1.4-fold higher biomethane, about 0.556 L/$L{\cdot}d$, was produced when the content of TS was controlled at 10 %, compared at 5 %, about 0.389 L/$L{\cdot}d$. When the swine manure was mixed with the corn silage or kitchen waste, a two-fold higher biomethane was produced, about 1.theand 1.0heL/$L{\cdot}d$, respectively, compared to the control treatment. Biogas production from organic dry matter (odm) was a3, 362eand 2h6 L/kg odm${\cdot}$d for control, corn silage, and kitchen waste treatment, respectively. The lower biogas production in the treatment of kitchen waste than that of corn silage is associated with its relatively high odm contents. The methane concentration during the whole process ranged from 40 at the beginning to 70 % at the end of process for both the control and kitchen waste treatments, and ranged from 52 to 70 % for the corn silage treatment. Hydrogen sulfide ($H_2S$) concentration ranged between 350 and 500 ppm. All the integrated results indicate that addition of organic byproduct into animal manure can double the generation of biogas from anaerobic fermentation process.

Extraction and Quality Stability of Products Containing Lilium Bulb and Lespedeza cuneata G. Don Extracts (백합과 비수리의 추출 및 추출혼합물이 함유된 음료 제품의 품질 안정성)

  • Kim, Seung Tae;Heo, Chang Hoe;Kim, Sung Hoon;Lee, Won Jong
    • Journal of Food Hygiene and Safety
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    • v.35 no.1
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    • pp.75-82
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    • 2020
  • The purpose of this study was to establish optimized extraction conditions for Lilium bulb and Lespedeza cuneata G. Don and to investigate the storage stability of beverages containing extracts. The hot-water extract and the 60% ethanol extract had the highest DPPH radical scavenging activities as well as the highest total polyphenol content. The total polyphenol content, total flavonoid content and DPPH radical scavenging activity were the highest when the Lilium bulb extract was mixed with the Lespedeza cuneata G. Don extract at the ratio of 1:4. Storage stability of beverages was determined during storage at 10, 25, and 35℃ for 6 months. The pH was decreased from 4.15 to 4.01-4.05, while the acidity was increased from 0.60% to 0.70-0.75% after storage for 6 months. The soluble solid contents were not changed during storage of 6 months. The DPPH radical scavenging activity was decreased after 4-6 months. The Hunter b (yellowness) values decreased at 35℃ after storage for 6 months while the lightness (L) and redness (a) were not changed during storage for 6 months. The total saponin content was not remarkably changed during 2 months of storage, while it decreased after 4-6 months of storage. The flavonoid content was decreased 47% and 55% from an intial 21.7 mg/100 mL to 10.3 mg/100 mL and 12.0 mg/100 mL after 1 month of storage and then remained stable until 6 months. General bacteria and coliform group were not detected during storage for 6 months.

The Quality Characteristics of Fermented and Soaked Jujube Wine (발효 및 침출 대추주의 품질 특성)

  • Kim, Soon-Jin;Chun, Myoung-Sook
    • The Korean Journal of Food And Nutrition
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    • v.23 no.3
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    • pp.332-341
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    • 2010
  • The chemical composition characteristics of jujube wine using different preparation methods including extraction in -soju (25%) and fermentation were investigated. The jujube wine was prepared by soaking in soju using- whole fruit(WJ-S1) or seed-removed fruit(WJ-S2). Fermentations of the jujube wine were started by using whole fruit(WJ-F1), seed-removed fruit(WJ-F2) or extracts of whole fruit(WJ-F3) after adding 24% sucrose. The pH of all samples decreased from a range of 4.92~5.42 at the start time to 3.66~4.97 after 100 days. The treatment WJ-F3 showed the lowest pH among all treatments. Total acid content was 0.67~1.01% at the initial stage and then changed to 0.51~0.88% after 100 days. Total sugar and reducing sugar contents were 1.20~13.8% and 0.50~4.45% at initial stage and then changed to 1.53~4.52% and 1.75~3.82% after 100 days respectively. These sugars decreased during the preparation and fermentation of the jujube wine. The amounts of free sugars including fructose, glucose, and sucrose ranged from 1.53~4.52% and treatment WJ-F3 showed the highest amount(1.95~13.64%) compared the other treatment. Glucose level were high in treatment WJ-S1 and WJ-S2, and fructose was increased in treatments WJ-F1, WJ-F2, and WJ-F3 after 60 days. Amino nitrogen content was 0.02~0.11% after 20 days and 0.07~0.14% after 40 days respectively. Solid content was 2.68~7.76% at the initial fermentation stage and changed to 4.81~9.73% after 100 days. Hunter color(L values) were 88.45~96.74 at the initial stage and then changed to 92.62~100.45 after 100 days. Preference tests a significant difference between the two types (extraction and fermentation) of jujube wine. And treatment WJ-S2 received the highest preference scores among the all treatments.

Immobilization of Burkholderia cepacia Lipase on Weak Base Styrene Resin Using Polyethyleneimine with Cross-linking (PEI(Polyethyleneimine)를 이용하여 음이온계 레진에 고정화된 Lipase AH 제조 및 효소적 Interesterification을 통한 반응 특성 연구)

  • Lee, Chi Woo;Lee, Ki Teak
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.7
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    • pp.1025-1035
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    • 2014
  • This study assessed the effect of immobilized lipase on weak base styrene resin using polyethyleneimine (PEI) with cross-linking. Two procedures were used in this study. The first one, "mono-layer" lipase immobilization, involves washing PEI after adsorption. The second procedure, "multi-layer" lipase immobilization, has no washing before the cross-linking step. Treverlite XS-100200 (weak base styrene resin) was immersed with PEI solution (2.2 mg/mL). Lipase AH (from Burkholderia cepacia) was adsorbed onto the support coated with PEI before cross-linking with glutaraldehyde. Structured lipid was synthesized by immobilized lipase-catalyzed interesterification using canola oil, palmitic ethyl ester (PEE), and stearic ethyl ester (StEE). Total fatty acid contents of triacylglycerol (TAG) in structured lipids were analyzed to investigate activity, properties, and reusability of immobilized lipases. Activities of immobilized lipases on the multi-layer and mono-layer increased at a high concentration (8 mg/mL) of lipase solution used for immobilization. The results show that immobilized lipase with the mono-layer method at pH 8.0 on resin had the highest total saturated fatty acid content (26.17 area%). Activity of immobilized lipase with the multi-layer method at pH 7.5 on support was lower than that of the mono-layer, but total saturated fatty acid content was 16.79 area% higher than that of lipase AH (15.01 area%).