• Title/Summary/Keyword: sole type

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Characteristics, Stability and Reisolation of nit Mutant of Fusarium oxysporum from Strawberry (딸기로부터 분리된 Fusarium oxysporum nit 변이주의 특성과 안정성 및 재분리)

  • 신동범;홍연규;조재민
    • Korean Journal Plant Pathology
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    • v.14 no.6
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    • pp.583-588
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    • 1998
  • This study was conducted to investigate the potential of nitrate-nonutilizing mutants (nit mutants) in ecological studies of Fusarium disease of strawberry. Nit mutants of Fusarium oxysporum from strawberry were easily formed on chlorate-containing media. Nit mutants were assigned to three phenotypic classes, nit1, nit3, and NitM, on the basis of their growth on media containing one of the following five different nitrogen sources ; nitrate, nitrite, hypoxanthine, ammonium and uric acid. Frequency of nit mutation and proportion of three phenotypes of nit mutants depended on the isolate. Mutation rate was 45.6% and ranged from 15.0% to 95.0%. The frequency of nit1 mutants was higher than that of nit3 or NitM. The complementary reaction between nit1 and NitM was higher than that of other combination. There has been no complementary response observed between nit3 and nit3. The nit mutants showed similar growth pattern as the that of wild type isolate on potato sucrose agar and potato sucrose liquid media. Most of the mutants retained pathogenicity, and maintained their phenotypes even after two year preservation through subculture on slanted PSA at room temperature. Nit mutants were selctively isolated from infested soil and infected plants on the selective medium (MMCPA) containing potassium chlorate with their original phenotypes, while naturally occurring isolates of Fusarium oxysporum were not grow on the medium. On the contrary, nit mutants showed very slight growth on the medium (MMPA) containing nitrate as a sole nitrogen source, and therefore could be distinguished from wild type isolate.

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Attribution of PAH Degradation of Sphingomonas chungbukensis DJ77 to the Plasmid pSY1 (Sphingomonas chungbukensis DJ77에 존재하는 Plasmid pSY1의 PAH 분해능)

  • 박승기;김성재;신희정;김영창
    • Korean Journal of Microbiology
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    • v.37 no.2
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    • pp.120-123
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    • 2001
  • Sphingomonas chungbukensis DJ77 is able to use phenanthrene and biphenyl as the sole carbon and energy source. Mitomycin C curing experiment suggested that polyaromatic hydrocarbon (PAH) utilization in strain DJ77 was plasmid-encoded. The plasmid cured strains were failed to grow on the minimal medium sprayed with biphenyl or phenanthrene. This was evident from southern hybridizations using a previously cloned DNA segment as a probe. There were positive signals in the palsmid DNA of the wild-type strain DJ77 and the absence of hybridizations with chromosomal DNA from the plasmid DNA of the wild-type strain DJ77 and the absence of hybridizations with chromosomal DNA from the palsmid-cured mutant strains.

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A Comparative Study of 3D DWT Based Space-borne Image Classification for Differnet Types of Basis Function

  • Yoo, Hee-Young;Lee, Ki-Won;Kwon, Byung-Doo
    • Korean Journal of Remote Sensing
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    • v.24 no.1
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    • pp.57-64
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    • 2008
  • In the previous study, the Haar wavelet was used as the sole basis function for the 3D discrete wavelet transform because the number of bands is too small to decompose a remotely sensed image in band direction with other basis functions. However, it is possible to use other basis functions for wavelet decomposition in horizontal and vertical directions because wavelet decomposition is independently performed in each direction. This study aims to classify a high spatial resolution image with the six types of basis function including the Haar function and to compare those results. The other wavelets are more helpful to classify high resolution imagery than the Haar wavelet. In overall accuracy, the Coif4 wavelet has the best result. The improvement of classification accuracy is different depending on the type of class and the type of wavelet. Using the basis functions with long length could be effective for improving accuracy in classification, especially for the classes of small area. This study is expected to be used as fundamental information for selecting optimal basis function according to the data properties in the 3D DWT based image classification.

Isolation and characterization of sodium dodecylbenzenesulfonate(soft type)-degrading bacteria (Sodium dodecylbenzenesulfonate(soft type)를 분해하는 미생물의 분리 및 특성)

  • 전홍기;안영희;백형석
    • Korean Journal of Microbiology
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    • v.27 no.3
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    • pp.279-284
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    • 1989
  • Macroorganisms capable of utilizing sodium dodecylbenzenesulfonate(SDBS, soft type) as a sole carbon source were isolated from nature by using SDBS agar plate technique. Iwolated bacteria were examined primarily for biodegradation ability of SDBS, and followed by testing for resistance to several kinds of metal compounds and antibiotics. Among them(152 strains), one strain showed a excellent SDBS-degrading ability with a resistance to amipicillin and rifampicin was selected. This bacterium was identified as Klebsiella sp. and harbored two plasmids of about 4 and 5 kilobases. SDBS-degrading ability was lost when the plasmids were cured by mitomycin C. It was revealed that the degradation of SDBS was controlled by the plasmid DNA encoding genes. The two plasmids were stably maintained in Escherichia coli C600.

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Study on the Determinants for the Type of New Venture Creation in Korea: Franchising or Independent Entrepreneurship (국내 프랜차이즈 창업과 독립 창업 집단의 결정 요인에 관한 연구)

  • Huh, Eun Jeong;Lee, Keon Hee
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.11 no.4
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    • pp.247-264
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    • 2016
  • The purpose of this study is to find the determinants for the type(Franchising or Independent Entrepreneurship) of new venture creation. This study conducted an empirical analysis on a total of 398 samples of survey gathered from people in Seoul, Gyoeng-gi, Daegu, and Gyeonsangbuk-do. This study includes not only personal traits, but also entrepreneurial intention and network as independent variables. Findings of the analysis reported that Entrepreneurial intention, Need for achievement, Autonomy, Entrepreneurship, Self-efficacy, Education, Network, Age, and Income have significant discriminant power, in order of priority, on general two groups of Franchising and Independent Entrepreneurship. However, in the study, autonomy is revealed as the sole discriminant factor on considering venture creation groups. Based on the result, the study contributes theoretical and practical implications in relation to the determinants for the type of franchising or independent entrepreneurship.

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Characteristics and Ring-Opening Isomerization Polymerization of 2-(1,3,3-Trimethyl-6-azabicyclo[3,2,1]-oct-6-yl)-4,5-dihydro-1,3-oxazoline (TAO) (2-(1,3,3-Trimethyl-6-azabicyclo[3,2,1]-oct-6-yl)-4,5-dihydro-1,3-oxazoline(TAO)의 개환이성화중합과 특성평가)

  • Lee, Chan-Woo;Chung, Jin-Do
    • Polymer(Korea)
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    • v.36 no.3
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    • pp.262-267
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    • 2012
  • 2-(1,3,3-Trimethyl-6-azabicyclo [3,2,1]-oct-6-yl)-4,5-dihydro-1,3-oxazoline (TAO) was polymerized at several conditions to clarify the influence of initiators, alkyl halide ($PhCH_2Br$, $PhCH_2Cl$, MeI) and sulfonate (MeOTf). The reactions were conducted at $100^{\circ}C$ for 24 h. The resultant polymer forms several kinds of structures with different combination of initiators. The sole MeOTf initiator caused chain transfer reaction to form the one-order structure for which the resultant polymer exclusively formed pendant structure, while alkyl halide and MeOTf formed two kinds of structures, pendant and main chain, which is caused by partly-proceeded double isomerization polymerization by highly reactive nucleophilic counter anion of halogen. Merrifield polymer was also utilized as an intiator and copolymerized with TAO, which produced a graft structure.

The Effect of $Clematidis$ Radix Herbal-acupuncture Solution, on Collagen, Adjuvant, Lipopolysaccharide and Phospholipase $A_2$ Induced Rheumatoid Arthritis in Mice (위령선약침이 Collagen, Adjuvant, LPS 및 PLA2 유발 류머티스성 관절염에 미치는 영향)

  • Lee, Jin-Seok;Kim, Kyung-Ho;Lee, Seung-Deok;Kim, Kap-Sung
    • Journal of Acupuncture Research
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    • v.29 no.1
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    • pp.127-137
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    • 2012
  • Objectives : The purpose of this study is to investigate the effect of $Clematidis$ radix herbal-acupuncture solution, on collagen, adjuvant, lipopolysaccharide and phospholipase A2 induced rheumatoid arthritis in mice. Methods : Arthritis index was measured for mouse that was injected subcutaneously in solution mixed chicken type II collagen with Freund's complete adjuvant. We injected Freund's complete adjuvant into right posterior part of the sole of a ICR mouse foot, which was measured by plethysmometer. The solution mixed $CRHS$ with Tris-HCI, $CaCl_2$, substrate, enzyme was done a chemical action for thirty minutes, and then inhibitory activity of PLA2 enzyme was expressed with inhibition percentage by utilizing isolated arachidonic acid. COX-2 was induced by adding LPS to RAW 264.7 cell, and COX-2 activity was measured by western blot analysis and $PGE_2$ Biotrak kit. Results : $CRHS$ also inhibited Freund's complete adjuvant induced chronic rheumatoid arthritis in mice. $CRHS$ showed significant inhibition of type I and type II $PLA_2$ activities in a dose dependent manner. Furthermore, $PGE_2$ production was decreased with $CRHS$ and lipopolysaccharide-induced COX-2 protein expression was significantly inhibited by $CRHS$. Conclusions : These results suggest that $CRHS$ has an therapeutic effect on drug induced-rheumatoic arthritis by inhibiting $PLA_2$ and COX-2 activities.

A Study on Changes in Biomechanical Characteristics of the Foot with Respect to Wedge-type Insole Thickness (키높이 인솔두께에 따른 족부의 생체역학적 특성변화에 대한 연구)

  • Park, T.H.;Jung, T.G.;Han, D.W.;Lee, Sung-Jae
    • Journal of Biomedical Engineering Research
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    • v.34 no.2
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    • pp.80-90
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    • 2013
  • Recently, functional insoles of wedge-type it is for the young to raise their height inserted between insole and heel cause foot pain and disease. Additionally, these have a problem with stability and excessively load-bearing during gait like high-heel shoes. In this study, we compared the changes in biomechanical characteristics of foot with different insole thickness then we will utilize for the development of the insole with the purpose of relieving the pain and disease. Subjects(male, n = 6) measured COP(center of pressure) and PCP(peak contact pressure) on the treadmill(140cm/s) using F-scan system and different insole thickness(0~50 mm) between sole and plantar surface during gait. Also, we computed changes of stresses at the foot using finite element model with various insole thickness during toe-off phase. COP moved anterior and medial direction and, PCP was increased at medial forefoot surface, $1^{st}$ and $2^{nd}$ metatarsophalangeal, ($9%{\uparrow}$) with thicker insoles and it was show sensitive increment as the insole thickness was increased from 40 mm to 50 mm. Change of the stress at the soft-tissue of plantar surface, $1^{st}$ metatarsal head represents rapid growth($36%{\uparrow}$). Also, lateral moments were increased over the 100% near the $1^{st}$ metatarsal as the insole thickness was increased from 0 mm to 30 mm. And it is show sensitive increment as the insole thickness changed 10 mm to 20 mm. As a result, it was expected that use of excessively thick insoles might cause unwanted foot pain at the forefoot region. Therefore, insole thickness under 30 mm was selected.

Analysis of Foot Characteristics According to the Classification of Foot Types of Junior High School Girls (여자 중학생의 발의 형태분류에 따른 유형별 특성 분석)

  • Lim, Ji-Young
    • Fashion & Textile Research Journal
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    • v.9 no.3
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    • pp.319-326
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    • 2007
  • The health of feet is connected with individual's health and affects a man's activity. Shoes need to be designed to protect feet and to absorb the impact of land. In order to choose suitable shoes for feet, the foot size and shape must be considered, so it is essential to grasp the exact size and shape of the foot. This study aims to present fundamental data on shoes' easy order prototype development for choosing shoes of good wearing comfort, by classifying feet size and shape junior high school boys in the early adolescent period. The subject were 217 Korean junior high school girls in age from 14 to 16 years old. The subjects were directly measured anthropometrically and indirectly analyzed photographically. 7 factors were extracted through factor analysis and those factors comprised 78.59% of total variance. The factors were characterized foot length, foot girth and width, foot shape around the fifth toes, foot shape around the first toes, angle of foot breadth, foot height, and foot length of upper foot. 3 clusters as their foot shape were categorized using 7 factor scores by cluster analysis. Type 1 had smaller in foot girth, width and length than other types and with deformed fifth toe. Type 2 had average size and high foot shape. Type 3 was characterized by long large foot with deformed first toe. The results would be a great support in producing and choosing appropriate shoes if forms are classified by subdividing foot form classification and extract a factor which shows only the foot sole shape.

Review on Case Reports of Syndrome differentiation and Herbal Medicine Treatment for Tic Disorder (틱 장애의 한의학적 변증 및 한약치료 치험례에 대한 고찰)

  • Lee, Ye Ji;Jeon, Ju Hyun;Kim, Eun seok;Kim, Young Il
    • Journal of Haehwa Medicine
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    • v.28 no.1
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    • pp.13-25
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    • 2019
  • Objectives : This study analyzed domestic case reports of syndrome differentiation and herbal medicine treatment for tic disorder published from 2000 to 2018 and aimed to seize the latest trend of Korean herbal medicine treatment according to syndrome differentiation. Methods : We searched herbal medicine treatment of tic disorder through five major Korean academic literature databases; NDSL, KTKP, KISS, RISS, DBPIA. Results : 12 articles were included in this study from 49 articles. These studies were analyzed by year, evaluation criteria, Korean treatments, treatment effects, syndrome differentiation, herbal medication, duration of medication, and type of herbal medicine. Treatment results were assessed by YGTSS. Most cases accompanied Korean integrative treatments and only one case report used herbal medicine alone. Most common type of syndrome differentiation for patients was spleen. Duration of medication varied according to each case and decoction was the most used type of herbal medication. Conclusions : It has been reported that herbal medicine treatment according to the syndrome differentiation could be effective in treating tic disorder. This study suggests that additional studies will be required to justify classification of syndrome differentiation and the sole effectiveness of herbal medicine on tic disorder.