• Title/Summary/Keyword: sole type

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Production of siderophore from L-glutamic acid as both carbon and nitrogen sole sources in Acinetobacter sp. B-W (글루탐산을 유일한 탄소원과 질소원으로 이용하는 Acinetobacter sp. B-W의 시드로포어 생산)

  • Kim, Kyoung-Ja;Jang, Ju-Ho;Yang, Yong-Joon
    • Korean Journal of Microbiology
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    • v.53 no.2
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    • pp.97-102
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    • 2017
  • Catechol type siderophore different from 2, 3-dihydroxybenzoic acid (DHB) was produced from Acinetobacter sp. B-W grown in medium containing L-glutamic acid as both carbon and nitrogen sole sources at $28^{\circ}C$. Optimal concentration of glutamic acid for siderophore production was 3% and production of siderophore was decreased above 3% glutamic acid. In previous report, siderophore, 2, 3-DHB was produced from strain B-W grown in medium containing glucose as carbon source and glutamic acid as nitrogen source. Rf value of siderophore produced from strain B-W grown in medium glutamic acid as both carbon and nitrogen sole sources at $28^{\circ}C$ was 0.32, while 2, 3-DHB was 0.84 in butanol-acetic acid-water (12:3:5) as developing solvent. Antioxidative activity of 2, 3-DHB was not detected in that siderophore produced from glutamic acid. Catechol nature of siderophore was detected by Arnow test. Although in iron-limited media optimal cell growth was identified at $36^{\circ}C$, significant quantities of siderophore were produced only at $28^{\circ}C$. Biosynthesis of siderophore was strongly inhibited by growth at $36^{\circ}C$. Production of siderophore was completely inhibited by $10{\mu}M\;FeCl_3$.

Microstructure Prediction Technology of Ni-Base Superalloy (단조용 니켈기지 초내열합금의 조직예측기술)

  • Yeom, J.T.;Kim, J.H.;Hong, J.K.;Park, N.K.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2009.10a
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    • pp.89-92
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    • 2009
  • As a class of materials, Ni-base superalloys are among the most difficult metal alloys to forge together with refractory metals and cobalt-base superalloys. The mechanical properties of Ni-base superalloys depend very much on grain size and the strengthening phases, $\gamma$' ($Ni_3$(Al,Ti)-type) and $\gamma$".($Ni_3$Nb-type). Especially, the control of grain size remains as a sole means for the control of mechanical properties. The grain size and distribution changes of the wrought superalloys during hot working and heat treatment are mainly controlled by the recrystallization and grain growth behaviors. In this presentation, prediction technology of grain size through the computer-aided process design, and numerical modeling for predicting the microstructure evolution of Ni-base superalloy during hot working were introduced. Also, some case studies were dealt with actual forming processes of Ni-base superalloys.

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Shade Avoidance and the Regulation of Leaf Inclination in Rice

  • Shin, Juhee;Park, Phun Bum
    • Rapid Communication in Photoscience
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    • v.3 no.3
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    • pp.53-55
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    • 2014
  • The shade avoidance syndrome is a morphological and physiological response when plants are exposed to shade. Recent work in Arabidopsis had begun to define the molecular components of the shade avoidance syndrome in dicotyledonous model plant. However, little is known about the shade avoidance response networks in agriculturally important monocotyledon crops such as rice. Here, we found that the degree of bending at the lamina joint is inversely proportional to the R:FR ratio. To elucidate which phytochrome is involved in this response, we did lamina joint inclination assay with the rice phytochrome-deficient mutants (osphyA, osphyB, and osphyC) and the wild type plants. Whereas the osphyA and osphyC knockout mutants bent at the lamina joint in the far-red rich condition as the wild type plants, the osphyB knockout mutants no longer bent at the lamina joint in the far-red rich condition. These results suggest that PHYB acts as a sole photoreceptor in the lamina joint inclination response in rice.

Selection of Nitrate-nonutilizing Mutants of Hypoxylon atropunctatum, A Fungal Pathogen on Oak Species

  • Chun, Se-Chul;Fenn, P.
    • The Plant Pathology Journal
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    • v.16 no.5
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    • pp.247-251
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    • 2000
  • Latent infections of healthy-appearing oaks of Hypoxylon atropunctatum complicates field studies by interfering with inoculation experiments to follow pathogenesis, fungal development and reproduction of this canker rot fungus. Mutants with unique and easily scorable phenotypes would be useful for inoculation studies. There is a broad range in the capacity of wild-type isolates to utilize nitrate as a sole nitrogen sources. Several types of nitrate-nonutilization mutants (nit1, Nit3, NitM) were selected from nitrate-utilizing wild-type isolates. Also, a few mutants of Hypoxylon atropunctatum were selected that could only grow poorly on basal medium supplemented with various nitrogen sources and even on yeast extract agar. These unknown mutants need to be characterized further. Nit mutants of Hypoxylon atropunctatum were readily selected, grew well and were recovered after inoculation into oak stems. These results suggest that nit mutants could be useful for inoculation studies in trees that contain latent infections.

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The Investigation of Rheological Properties Development for Polymer Matrix Including Foaming Agent

  • Lee, Seung Hak;Kim, Dong Gun;Lim, Sung Wook;Park, Eun Young;Park, Tae Sun;Hyun, Kyu
    • Elastomers and Composites
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    • v.51 no.1
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    • pp.24-30
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    • 2016
  • Sole in the footwear usually modified with foaming agent on the polymer resin to improve the lightweightness and crush-cushion effect. In this study, we investigated rheological properties for polymer resin filled with the different type and concentration of foaming agent, capsule type foaming agent and organo-chemical foaming agent, under the time sweep test. Curing times of each polymer resin with different kind of foaming agent are delayed than reference material (epoxy resin with curing agent). In case of adding capsule type foaming agent, however, there is appropriate concentration to reduce the curing time, relatively. When foaming agent is activated, foaming force inflates the sample in contrast to condensation force of curing and then axial normal force develop to the (+) direction. Interestingly, by increase concentration of foaming agent, there is a specific point to break down the axial normal force development. The reason for this phenomenon is that coalescence of foams induce the blocking of axial normal force development.

Selective Isolation and Characterization of Schwanniomyces castellii Mutants with Increased Production of a-Amylase and Glucoamylase

  • Ryu, Yeon-Woo
    • Journal of Microbiology and Biotechnology
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    • v.3 no.2
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    • pp.95-98
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    • 1993
  • This study was carried out to isolate and characterize the mutant strains of Schwanniomyces castellii NRRL Y-2477. Mutants were prepared with the treatment of ethyl methane sulfonate. 2-deoxy-D-glucose resistant mutants were isolated and two mutants were selected based on their high production of amylolytic enzymes and their ability to ferment starch. The mutants selected had higher a-amylase and glucoamylase activities than the wild type strain from several other carbon sources. Especially, it was revealed that mutant strain M-9, when cultured in the presence of glucose as a sole carbon source, shows relatively high activities of a-amylase and glucoamylase compared to those of the wild type strain. In result, this mutant strain can be considered as a constitutive producer of amylolytic enzymes. To compare the ethanol production ability of wild type strain and of mutant strains selected, an alcohol fermentation was carried out using 100 g/l soluble starch. Mutant strain M-9 did not improve the direct alcohol fermentation of starch, despite its excellent amylolytic activities performance. On the other hand, mutant strain M-6 produced 37.9 g/l (4.8%, v/v) ethanol by utilizing about 82% of substrate.

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Biodegradation of crude oil hydrocarbons by Acinetobacter sp. isolated from activated sludge (활성슬러지에서 단리한 Acinetobacter sp.에 의한 원유탄화수소분해)

  • Dong-Hyuk CHOI;Dong Hoon LEE
    • Journal of Korea Soil Environment Society
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    • v.5 no.1
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    • pp.97-108
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    • 2000
  • A Gram-type negative bacteria that can utilize crude oil as the sole source of carbon and energy was isolated from an activated sludge of a local sewage treatment plant and identified tentatively as belonging to the genus Acinetobacter. The isolate could degrade n-alkanes and unidentified hydrocarbons in crude oil and utilize n-alkanes, hydrophobic substrates, as sole carbon and energy sources. n-Alkanes from tridecane (Cl3) to triacontane (C30) in crude oil were degraded simultaneously with no difference in degradation characteristics between the two close odd and even numbered alkanes in carbon numbers. The linear growth of the isolate and the degradation characteristics of Pr-alkanes suggested that the transport of substrates from the oil phase to the site where the substrates undergo the initial oxidation in microorganism might be the rate limiting in the biodegradation process of crude oil constituents. The remainder fraction of substrates after cultivation was considered to reflect the hydrocarbon inclusions in the cell mass, characteristics in Acinetobacter species, and to control the transport of substrates from crude oil phase. On the basis of the results, the isolate was considered to play an important role in the degradation study of hydrophobic environmental pollutants.

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A study of Agricultural fatigue shoes - A comparative study of heat load by shoe type - (농작업화에 관한 연구 - 신발종류에 따른 열적 부담 비교연구 -)

  • 이경숙;최정화
    • Korean Journal of Rural Living Science
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    • v.7 no.2
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    • pp.99-108
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    • 1996
  • This study has intended to suggest fundamental data to develope and choose appropriate shoes for upland farming in order to prevent health deterioration of women workers and improve work effectiveness and reduce fatigue by wearing appropriate shoes. During 1995. 4. 28 - 5. 10, Fifty women workers in hot pepper farming were observed and major shoe types, which were rubber shoes, walking shoes, slippers, and rubber boots, were selected for the study. During 1995. 10. 9 - 31, two subjects were tested by wearing those shoes in the laboratory where the temperature was 24$\pm$1$^{\circ}C$ and relative humidity 50$\pm$5%RH. And the temperature & humidity on sole and in the shoes, the rectal temperature, skin temperature, blood pressure, pulse, lactate concentration of blood, Flickers' value and subjective sensation were measured. The results were as follows : 1. 84% of women workers mentioned that they need shoes improvement and the order of most frequent shoe types to be worn was rubber shoes, walking shoes, slippers, rubber boots. 2. The rate of women who were unsatisfied with shoes for upland farming is 38 percentages. The reason of unsatisfaction was that feet were in a sweat and alien substances were let into shoes. 3. The temperature & humidity on sole were the lowest in rubber boots during experiment(p<0.01). 4. The relative humidity in the shoes was the highest in rubber boots by 90% and the lowest in walking shoes by 72% during rest And the humidity in slippers and walking shoes were significantly low in experiment(p<0.001). 5. Rubber boots showed the highest rise in rectal temperature by 0.2$^{\circ}C$ showing increase of core temperature (p<0.05). 6. The mean skin temperature during experiment was highest in rubber boots by 33.8$^{\circ}C$(p<0.001).

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Study on the Thermal and Dynamic Behaviors of Air Spring for vibration isolation of LCD panel inspecting machine connected with an External Chamber through a flexible tube: PART I, Theoretical Modeling (외부챔버와 유연한 튜브로 연결된 LCD 패널 검사기 방진용 공기 스프링의 열 및 동적 연성거동에 대한 연구: PART I, 이론적 모델링)

  • Seok, Jong-Won;Lee, Ju-Hong;Kim, Pil-Kee
    • Journal of the Semiconductor & Display Technology
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    • v.10 no.1
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    • pp.33-41
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    • 2011
  • Due to the recent quantum leaps forward in bio-, nano-, and information-technologies (BT, NT and IT), the precisionization and miniaturization of mechanical and electrical components are in high demand. In particular, the ITrelated equipments that take a great part in our domestic industry are in the area requiring high precision technologies. As a consequence, the researches on the development vibration isolation systems that diminish external disturbance or internal vibration are highly required. Among the components comprising the vibration isolation system, air spring has become on a focal point for the researchers due to its merits. This air spring is able to support heavy loads, keep a low natural frequency despite of having a lower value of stiffness, and control the performance of vibration isolation. However, sometimes the sole use of air spring is in demand due to some economic reasons. Under this circumstance, the damping effect of sole air spring may not enough to reduce sufficient amount of vibration. In this study, the air spring mount system connecting with an external chamber is proposed to increase or control the damping effect. To investigate its damping mechanism, the thermal and dynamic behaviors of the system is examined through a theoretical modeling approach in this part of research. In this approach, thermomechanical and Helmholtz resonator type models are to be employed for the air spring/external chambers and connecting tube system, respectively. The frequency response functions (FRFs) derived from the modeling effort are evaluated with physical parametric values and the effects of connecting tube length on these FRFs are identified through computer simulations.

A study on the characteristics of color application indicated from urban housing complex - A Forcuse on the Kirchsteigfeld Housing Complex in Potsdam City - (도시주거단지에 나타난 색채사용특성에 관한 연구 - 독일 포츠담시 Kirchsteigfeld 주거단지를 중심으로 -)

  • Lee, Seon-Min;Lee, Min-Seok
    • Korean Institute of Interior Design Journal
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    • v.16 no.6
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    • pp.250-257
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    • 2007
  • Characteristics of color application indicated from Kirchsteigfeld is presented the various points of view to be preserved urban housing color. It had been handled as environmental color by view point of community and connectivity rather than color scheme of single housing. First, color to be consisted of basis for urban entirely as background color in urban housing complex has been indicated the bright and cheerful feelings and expressed to be introduced mostly from the single color system. Second, characteristics to be inhibited strongly from housing complex had been indicated by restricted four types of color, formulated the overall context and change through accent and the change from disposition of principal and the accessary of color scheme. In this case, it was not regular formulation, but as a whole category view, it could be provided by orderly regularity system strictly Particularly it had been applied as a whole for lightness change by single color, introduced the different color of building to be centered or remained by a visual point. This had been formed to introduction of color by stand points of housing group not introduction to color by sole concept of building. Third, emphasizing of morphologic feeling by using of difference of lightness, it could be indicated the mild and comfortable stereo-type sensibility from the overall types of housing, we could understand for formation of color code by lightness of slightly differences. Fourth, it had been taken the convenient adaptation method for maintenance of simple graphic system as a environmental color. It has considerable advantage for sustainability of color to indicate on finishing material, form and detail in architecture, as the sole separated color not to be complicated when to be painted over and again or managed continuously.