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http://dx.doi.org/10.7845/kjm.2017.7023

Production of siderophore from L-glutamic acid as both carbon and nitrogen sole sources in Acinetobacter sp. B-W  

Kim, Kyoung-Ja (Department of life Science and Biotechnology, College of Natural Science, Soonchunhyang University)
Jang, Ju-Ho (Department of life Science and Biotechnology, College of Natural Science, Soonchunhyang University)
Yang, Yong-Joon (Department of Plant and Food Science, Sangmyung University)
Publication Information
Korean Journal of Microbiology / v.53, no.2, 2017 , pp. 97-102 More about this Journal
Abstract
Catechol type siderophore different from 2, 3-dihydroxybenzoic acid (DHB) was produced from Acinetobacter sp. B-W grown in medium containing L-glutamic acid as both carbon and nitrogen sole sources at $28^{\circ}C$. Optimal concentration of glutamic acid for siderophore production was 3% and production of siderophore was decreased above 3% glutamic acid. In previous report, siderophore, 2, 3-DHB was produced from strain B-W grown in medium containing glucose as carbon source and glutamic acid as nitrogen source. Rf value of siderophore produced from strain B-W grown in medium glutamic acid as both carbon and nitrogen sole sources at $28^{\circ}C$ was 0.32, while 2, 3-DHB was 0.84 in butanol-acetic acid-water (12:3:5) as developing solvent. Antioxidative activity of 2, 3-DHB was not detected in that siderophore produced from glutamic acid. Catechol nature of siderophore was detected by Arnow test. Although in iron-limited media optimal cell growth was identified at $36^{\circ}C$, significant quantities of siderophore were produced only at $28^{\circ}C$. Biosynthesis of siderophore was strongly inhibited by growth at $36^{\circ}C$. Production of siderophore was completely inhibited by $10{\mu}M\;FeCl_3$.
Keywords
Acinetobacter sp. B-W; L-glutamic acid; siderophore; sole carbon and nitrogen sources;
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