• Title/Summary/Keyword: soil sampling

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The distribution of protozoans according to soil pollution around Ulsan industrial complexes, Korea (울산공단 주변의 토양오염에 따른 원생동물의 분포)

  • 신만균;최기룡
    • Journal of Environmental Science International
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    • v.5 no.2
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    • pp.187-194
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    • 1996
  • The present study was performed to elucidate the distribution of protozoans according to the actual conditions of soil pollution around Ulsan industrial complexes, Korea. Samples were collected from the top-soils of 13 localities in eight times during the period from 16 April 1994 to 14 January 1995. As a result of this study, total 11 species of hypotrichous ciliated protozoa were identified and analyzed. These hypotrichs are 6 species of stichotrichine hyporichs ( Keronopsis sp., Pseudourostyla sp., Hoiriiticha syivatica, Hipiosticha multistylata, Hoios-ticha sp. and ParuroleFitus sp.) and 5 species of sporadotrichine hypotrichs (Oxyricha sp., Steinla sp., Histriculus cavicola, Hemisincirra sp. and Gonostomum auiili). Of these 11 species, 4 species (Keronopsis sp., Pseudourostyla sp., Holosticha sp. and Hemisincirra sp.) are reriorted for 1%e first time from Korea. All the sampling localities could be grouped in three zones by the cluster analysis with the abundance and distribution of protozoans. This result is approximately coincide with the ionation by the concentration of heavy metals.

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Analysis on the Structure of Plant Community in Mt. Yongmun by Classification and Ordination Techniques (Classification 및 Ordination 방법에 의한 융문산 삼림의 식물군집 구조분석)

  • 이경재
    • Journal of Plant Biology
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    • v.33 no.3
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    • pp.173-182
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    • 1990
  • To investigate the structure of the plant community structure of Mt. Yongmun in Kyonggi-do, fifty-four plots were set up by the clumped sampling method. The classification by TWINSPAN and DCA ordination were applied to the study area in order to classify them into several groups based on woody plant and environmental variables. By both techniques, the plant community were divided into two groups by the aspect. the dominant species of south aspect were Pinus densiflora, Quercus aliena, Q. mongolica, Carpinus laxiflora and of north aspect were Q. ongolica, Fraxinus rhynchophylla. The successional trends of tree species in south aspect seem to be from P. densiflora through Q. serrata, Q. aliena, A. mongolica to C. laxiflora. As a result of the analysis for the relationship between the stand scores of DCA and environmental variables, they had a tendency to increase significantly from the P. densiflora and Q. mongolica community to C. laxiflora and F. rhynchophylla community that was the soil moisture, the amount of soil humus and soil pH.

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Metagenomic Analysis of Fungal Communities Inhabiting the Fairy Ring Zone of Tricholoma matsutake

  • Kim, Miae;Yoon, Hyeokjun;You, Young-Hyun;Kim, Ye-Eun;Woo, Ju-Ri;Seo, Yeonggyo;Lee, Gyeong-Min;Kim, Young Ja;Kong, Won-Sik;Kim, Jong-Guk
    • Journal of Microbiology and Biotechnology
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    • v.23 no.10
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    • pp.1347-1356
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    • 2013
  • Tricholoma matsutake, an ectomycorrhiza that has mutual relationships with the rootlet of Pinus denisflora, forms a fruiting body that serves as a valuable food in Asia. However, the artificial culture of this fungus has not been successful. Soil fungi, including T. matsutake, coexist with many other microorganisms and plants; therefore, complex microbial communities have an influence on the fruiting body formation of T. matsutake. Here, we report on the structures of fungal communities associated with the fairy ring of T. matsutake through the pyrosequencing method. Soil samples were collected inside the fairy ring zone, in the fairy ring zone, and outside the fairy ring zone. A total of 37,125 sequencing reads were obtained and 728 to 1,962 operational taxonomic units (OTUs) were observed in the sampling zones. The fairy ring zone had the lowest OTUs and the lowest fungal diversity of all sampling zones. The number of OTUs and fungal taxa inside and outside the fairy ring zone was, respectively, about 2 times and 1.5 times higher than the fairy ring. Taxonomic analysis showed that each sampling zone has different fungal communities. In particular, out of 209 genera total, 6 genera in the fairy ring zone, such as Hemimycena, were uniquely present and 31 genera, such as Mycena, Boletopsis, and Repetophragma, were specifically absent. The results of metagenomic analysis based on the pyrosequencing indicate a decrease of fungal communities in the fairy ring zone and changes of fungal communities depending on the fairy ring growth of T. matsutake.

Application of In Situ Measurement for Site Remediation and Final Status Survey of Decommissioning KRR Site

  • Hong, Sang Bum;Nam, Jong Soo;Choi, Yong Suk;Seo, Bum Kyoung;Moon, Jei Kwon
    • Journal of Radiation Protection and Research
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    • v.41 no.2
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    • pp.173-178
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    • 2016
  • Background: In situ gamma spectrometry has been used to measure environmental radiation, assumptions are usually made about the depth distribution of the radionuclides of interest in the soil. The main limitation of in situ gamma spectrometry lies in determining the depth distribution of radionuclides. The objective of this study is to develop a method for subsurface characterization by in situ measurement. Materials and Methods: The peak to valley method based on the ratio of counting rate between the photoelectric peak and Compton region was applied to identify the depth distribution. The peak to valley method could be applied to establish the relation between the spectrally derived coefficients (Q) with relaxation mass per unit area (${\beta}$) for various depth distribution in soil. The in situ measurement results were verified by MCNP simulation and calculated correlation equation. In order to compare the depth distributions and contamination levels in decommissioning KRR site, in situ measurement and sampling results were compared. Results and Discussion: The in situ measurement results and MCNP simulation results show a good correlation for laboratory measurement. The simulation relationship between Q and source burial for the source layers have exponential relationship for a variety depth distributions. We applied the peak to valley method to contaminated decommissioning KRR site to determine a depth distribution and initial activity without sampling. The observed results has a good correlation, relative error between in situ measurement with sampling result is around 7% for depth distribution and 4% for initial activity. Conclusion: In this study, the vertical activity distribution and initial activity of $^{137}Cs$ could be identifying directly through in situ measurement. Therefore, the peak to valley method demonstrated good potential for assessment of the residual radioactivity for site remediation in decommissioning and contaminated site.

Sample Disturbance due to Sampling Techniques and Comparison of Consolidation Parameters Between Testing Methods on Pusan Clays (부산점토에 대한 시료채취 기술에 따른 시료교란과 시험방법에 따른 압밀정수의 비교)

  • 곽정민;정성교;백승훈;이영남;조기영
    • Journal of the Korean Geotechnical Society
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    • v.19 no.4
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    • pp.43-53
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    • 2003
  • To get improved soil parameters in the laboratory tests, the effects of sample disturbance should be excluded as much as possible. A collaborative research between Dong-A University and PHRI (Japan) was conducted to investigate the effects of sample disturbance due to sampling techniques and samplers on Pusan clays. The results of unconfined compression and consolidation tests performed on the clay samples taken by them have been compared in terms of soil parameters and sample disturbance. As the result of the study, it was found that when the Korean sampler was used with a different technique of cleaning the borehole bottom and removing slime before inserting and penetrating the sampler tube, similar to the one commonly used in Japanese practice, the quality of samples could be greatly improved. Furthermore, the CRS test with the rate of 0.02%/min yielded larger values of consolidation parameters and better degree of sample disturbance than those of oedometer test, due to the difference in testing method.

Differential Impacts on Bacterial Composition and Abundance in Rhizosphere Compartments between Al-Tolerant and Al-Sensitive Soybean Genotypes in Acidic Soil

  • Wen, Zhong-Ling;Yang, Min-Kai;Fazal, Aliya;Liao, Yong-Hui;Cheng, Lin-Run;Hua, Xiao-Mei;Hu, Dong-Qing;Shi, Ji-Sen;Yang, Rong-Wu;Lu, Gui-Hua;Qi, Jin-Liang;Hong, Zhi;Qian, Qiu-Ping;Yang, Yong-Hua
    • Journal of Microbiology and Biotechnology
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    • v.30 no.8
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    • pp.1169-1179
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    • 2020
  • In this study, two soybean genotypes, i.e., aluminum-tolerant Baxi 10 (BX10) and aluminumsensitive Bendi 2 (BD2), were used as plant materials and acidic red soil was used as growth medium. The soil layers from the inside to the outside of the root are: rhizospheric soil after washing (WRH), rhizospheric soil after brushing (BRH) and rhizospheric soil at two sides (SRH), respectively. The rhizosphere bacterial communities were analyzed by high-throughput sequencing of V4 hypervariable regions of 16S rRNA gene amplicons via Illumina MiSeq. The results of alpha diversity analysis showed that the BRH and SRH of BX10 were significantly lower in community richness than that of BD2, while the WRH exhibited no significant difference between BX10 and BD2. Among the three sampling compartments of the same soybean genotype, WRH had the lowest community richness and diversity while showing the highest coverage. Beta diversity analysis results displayed no significant difference for any compartment between the two genotypes, or among the three different sampling compartments for any same soybean genotype. However, the relative abundance of major bacterial taxa, specifically nitrogen-fixing and/or aluminum-tolerant bacteria, was significantly different in the compartments of the BRH and/or SRH at phylum and genus levels, indicating genotype-dependent variations in rhizosphere bacterial communities. Strikingly, as compared with BRH and SRH, the WRH within the same genotype (BX10 or BD2) always had an enrichment effect on rhizosphere bacteria associated with nitrogen fixation.

In-situ monitoring and reliability analysis of an embankment slope with soil variability

  • Bai, Tao;Yang, Han;Chen, Xiaobing;Zhang, Shoucheng;Jin, Yuanshang
    • Geomechanics and Engineering
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    • v.23 no.3
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    • pp.261-273
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    • 2020
  • This paper presents an efficient method utilizing user-defined computer functional codes to determine the reliability of an embankment slope with spatially varying soil properties in real time. The soils' mechanical properties varied with the soil layers that had different degrees of compaction and moisture content levels. The Latin Hypercube Sampling (LHS) for the degree of compaction and Kriging simulation of moisture content variation were adopted and programmed to predict their spatial distributions, respectively, that were subsequently used to characterize the spatial distribution of the soil shear strengths. The shear strength parameters were then integrated into the Geostudio command file to determine the safety factor of the embankment slope. An explicit metamodal for the performance function, using the Kriging method, was established and coded to efficiently compute the failure probability of slope with varying moisture contents. Sensitivity analysis showed that the proposed method significantly reduced the computational time compared to Monte Carlo simulation. About 300 times LHS Geostudio computations were needed to optimize precision and efficiency in determining the failure probability. The results also revealed that an embankment slope is prone to high failure risk if the degree of compaction is low and the moisture content is high.

Water Saving Irrigation Standard of Tomato in Greenhouse

  • Eom, Ki-Cheol;Lee, Byung-Kook;Koh, Mun-Hwan;Eom, Ho-Yong;Sonn, Yeun-Kyu
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.5
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    • pp.313-321
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    • 2013
  • The Average daily PET (Potential evapotranspiration), evaluated based on the last 30 years meteorological data and the lysimeter experiment carried out by RDA during 11 years, of 9 regions in Korea for the tomato cultivated in greenhouse, was $3.41mm\;day^{-1}$. Two kinds of water saving irrigation standard (WSIS), deficit irrigation standard (DIS) and partial root-zone drying irrigation standard (PRDIS) that include the irrigation interval and the amount of irrigation water according to the region, soil texture and growing stage, were established. According to the DIS and PRDIS, the cultivator can save water up to 29.2% and 53.7%, respectively, for tomato cultivation in greenhouse compared to the full irrigation standard (FIS) which established in 1999. WSIS can be used easily by the cultivator without complicate procedures such as soil sampling and measurement of soil water status by expensive sensors. But the cultivator should care about irrigation method such as PRDI (partial root-zone drying irrigation) without yield decrease.

A Study on the Extent of the Pollution of an Illegal Landfill (불량폐기물 매립지의 오염에 관한 고찰)

  • 김동우;박성원;이재영;이평구
    • Journal of Soil and Groundwater Environment
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    • v.6 no.2
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    • pp.31-38
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    • 2001
  • An illegal landfill In Korea was evaluated in a view of environmental quality to figure out reusing the area. The sampling spots were selected at a point in the landfill and three points in it's around area(two points in a paddy soils and one in a farm field). The eleven pollutants regulated in the Korean Soil Environmental Preservation Act (KSEPA) were analysed from the soil sample with the method of the KSEPA. As the results of the analysis the level of concentration with most samples indicated the requirement of the KSEPA. The concentration of soil samples met the standard: however, some pollutants in the ground water and leachate showed higher concentration than that of the requirement. Therefore, we must consider the treatments of the pollutants in the ground water and leachate when designing this landfill area for reusing.

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Influence of Varying Degree of Salinity-Sodicity Stress on Enzyme Activities and Bacterial Populations of Coastal Soils of Yellow Sea, South Korea

  • Siddikee, Md. Ashaduzzaman;Tipayno, Sherlyn C.;Kim, Ki-Yoon;Chung, Jong-Bae;Sa, Tong-Min
    • Journal of Microbiology and Biotechnology
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    • v.21 no.4
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    • pp.341-346
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    • 2011
  • To study the effects of salinity-sodicity on bacterial population and enzyme activities, soil samples were collected from the Bay of Yellow Sea, Incheon, South Korea. In the soils nearest to the coastline, pH, electrical conductivity ($EC_e$), sodium adsorption ratio (SAR), and exchangeable sodium percentage (ESP) were greater than the criteria of saline-sodic soil, and soils collected from sites 1.5-2 km away from the coastline were not substantially affected by the intrusion and spray of seawater. Halotolerant bacteria showed similar trends, whereas non-tolerant bacteria and enzymatic activities had opposite trends. Significant positive correlations were found between EC, exchangeable $Na^+$, and pH with SAR and ESP. In contrast, $EC_e$, SAR, ESP, and exchangeable $Na^+$ exhibited significant negative correlations with bacterial populations and enzyme activities. The results of this study indicate that the soil chemical variables related with salinity-sodicity are significantly related with the sampling distance from the coastline and are the key stress factors, which greatly affect microbial and biochemical properties.