• Title/Summary/Keyword: soil samples

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Microbial Diversity in the Soil Damaged by a Forest Fire (훼손된 산불토양의 미생물다양성)

  • Jung, Young-Ryul;Song, In-Geun;Kim, Jin-Yong;Lee, Sin-Geun;Kim, Young-Jun
    • Journal of the Korea Organic Resources Recycling Association
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    • v.13 no.2
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    • pp.85-90
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    • 2005
  • Changes of biochemical and genetic diversity of microbial communities in the soil damaged by a forest fire were analyzed. Soil samples were collected from Gangnung area where a forest fire was broken out in 2000. Two soil samples were from the burnt area, one from the naturally restoring soil (NS) and the other from the artificially restoring soil (AS). A normal, unaffected soil sample (US) was also included as a control. For the biochemical diversity, each sample was directly applied to the BIOLOG system, and the cluster analysis through a statistic process (SPSS) were performed. Genetic diversity was analyzed through DGGE using 16S-rDNA amplified from soil DNA. Among the samples tested, top soils of US and NS, and sub soil of NS revealed more than 70% of the similarity value in biochemical diversity. In case of genetic diversity, however, the similarity value was found to be in the range of 53% to 68% in all samples. This result indicates that the biochemical diversity is not always correlated with the genetic diversity in the analysis of microbial communities.

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Physical and Chemical Characteristics of Dokdo Soil

  • Lee, Gil-Seong;Choo, Yeon-Sik
    • Journal of Ecology and Environment
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    • v.32 no.4
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    • pp.295-304
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    • 2009
  • To understand the properties of soil in Dokdo, we collected soil samples from 12 locations on Seodo and 23 locations on Dongdo, in Dokdo of Gyeongsangbuk-do Province in 2007-2008 and analyzed the soil's physical and chemical characteristics. Sand comprises the largest component (49.37%) of Dokdo soil, followed by silt (40.70%) and clay (9.93%). The soil structure consists mostly of sand loam, followed by loam and silt loam. The pH level of soils from Dokdo varied dramatically among sampling sites and seasons, ranging from 3.36 to 8.02. The total ion content of Dokdo soil also varies greatly among survey places and periods, but in general the total ion content was high in summer when vegetation develops, and low in spring. The exchangeable cation contents of the soil showed low levels in samples where the soil pH was low, including habitats dominated by Agropyron tsukushiense var. transiens and Echinochloa crus-galli, whereas the exchangeable cation contents were high where the organic contents were high, as in habitats dominated by Liriope platyphylla and Artemisia japonica subsp. littoricola. Soil N contents varied greatly among survey sites and higher N contents were found in soil inhabited by Chenopodiaceous plants than in habitats inhabited by other plants. The substantial differences in phosphorus contents among sites were related to excrement of black-tailed gulls. To understand the basic physical and chemical features of the soil on Dokdo, it will be necessary to conduct seasonal and long-term research on soil pH, ion contents, organic contents, N and P, as well as obtaining precise data from samples collected at different depths.

Status and changes in physico-chemical properties of soil in Chungcheongnam-do

  • Yun-Gu Kang;Sung-Jin Park;Jae-Han Lee;Jin-Hyuk Chun;Jun-Young Lee;Taek-Keun Oh
    • Korean Journal of Agricultural Science
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    • v.49 no.2
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    • pp.239-247
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    • 2022
  • The physico-chemical properties of agricultural soil are factors that affect crop productivity and soil fertility. In Korea, agricultural environment change surveys have been conducted every four years since 1999. The purpose of this study is to investigate the status and changes in the physico-chemical properties of agricultural soil in Chungcheongnam-do. Samples were collected from the exact location of the aforementioned environment survey, and environmental variables (land use, type of crop cultivated) were investigated in relation to the samples. Soil collection was conducted using a core sampler and a single gouge auger. The bulk density of the soil generally increased up to a depth of sampling of 40 cm but decreased thereafter to a depth of 60 cm. Additionally, the bulk density was highest in the upland soil case and stood at 1.59 g·cm-3, while the lowest value of 1.52 g·cm-3 was obtained from orchard soil samples. Conversely, the porosity and moisture gradually decreased at soil depths of 0 - 40 cm and increased at depths of 40 - 60 cm. Most of the soil chemical properties generally decreased with an increase the soil depth from 0 to 70 cm, but electrical conductivity (EC) increased up to a depth of 40 cm. Therefore, it is judged that it is necessary to lower the bulk density by supplying organic matter for agricultural land in Chungcheongnam-do.

LEAD LEACHABILITY FROM QUICKLIME TREATED SOILS IN A DIFFUSION CONTROLLED ENVIRONMENT

  • Moon, Deok-Hyun
    • Environmental Engineering Research
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    • v.10 no.3
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    • pp.112-121
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    • 2005
  • The effectiveness of quicklime-based stabilization/solidification (S/S) in immobilizing lead (Pb) was assessed by performing semi-dynamic leaching tests (ANS16.1). In order to simulate landfill leaching conditions, the ANS 16.1 test was modified by using 0.014 N acetic acid (pH = 3.25) instead of distilled water. Artificial soil samples as well as field soil samples contaminated with Pb were tested. The effectiveness of quicklime treatment was evaluated by determining diffusion coefficients ($D_e$) and leachability indices (LX). A model developed by de Groot and van der Sloat was used to elucidate the controlling Pb leaching mechanisms. Overall, upon quicklime treatment Pb leachability was significantly reduced in a]l of the samples tested. The mean LX values were higher than 9 for an artificial soil sample containing 30% kaolinite treated with 10% quicklime and for a field soil sample treated with 10% quicklime, which suggests that S/S treated soils can be considered acceptable for "controlled utilization". Moreover, quicklime treatment was more effective in artificially contaminated soil with high kaolinite content (30%), indicating the amount of clay plays an important role in the success of the treatment. The controlling Pb leaching mechanism was found to be diffusion, in all quicklime treated samples.

A Survey of Parasitic Eggs in Soils and Vegetables in a Korean Rural Area (일부농촌지역의 토양 및 채소류에서의 기생충란 조사)

  • 정문식
    • Journal of Environmental Health Sciences
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    • v.2 no.1
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    • pp.1-4
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    • 1975
  • The law prohibits use of human excreta without proper treatment since 1968 in Korea, but the rate of infection of parasitic diseases is high among all ages of people. An examination of parasitic eggs in soil and vegetables was performed from April to September, 1974, soil samples were collected near toilet, at gardens, at vegetable farms with excreta used and not used, and strawberry farms in Shindong myon, Chunseong-gun, Kangwon province, and vegetables collected were cabbage, young radish, lettuce, Welshonion, spinach and strawberry. Main findings are as follows 1. Positive samples of Ascaris lumbricoides are 18 out of 25(64%) in the soils near toilet, 9 out of 28(3.2%) in the garden soils and 8 out of 18(44%) in the soils of vegetable farms. 2. Trichocephalus trichiurus and Enterobius vermicularis were detected in 8 soil samples respectively Trichostrongylus orientalis 7(25%) and Ancylostoma duodenale 3(11%) out of in the soils near toilets. 3. Parasitic egg negatives are 62(55%) out of 112 soil samples. 4. Ascaris lumbricoides is found in 11 roots(48%) of cabbage and young radish out of 23 respectively, 14 lettuce(34%) out of 41,4 Welsh onion(40%) out of 10,3 spinach(33%) out of 9, and 2 strawberry(11%) out of 18. 5. Ancylostoma duodenale positives are 2 roots of cabbage(9%) out of 23, 4 young radish (17%) out of 23, and 7 lettuce(17%) out of 41, and negatives of parasitic eggs on vegetables are 62 roots(51%) out of 124 samples.

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Hydrogeological Characterization of Petroleum Contaminated area in Kangwon (강원 유류오염지역의 수리지질 특성 연구)

  • Choi, Hyun-Mi;Lee, Jin-Yong
    • Journal of Soil and Groundwater Environment
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    • v.15 no.2
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    • pp.1-9
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    • 2010
  • Estimations of porosity and bulk density, particle size analysis of soli samples, tracer test and slug test were performed in a petroleum contaminated area of Kangwon for understanding characteristics of the aquifer. Porosities of the samples were estimated 0.158~0.257, and bulk densities were estimated as $1.73\sim2.10\;g/cm^3$. Majority proportion of the soil samples was 0.5~1.0 mm size. In the soil texture triangle, all samples were distributed at sand area. Uniformity coefficients were estimated as 7.71~10.39, and thus all samples were poorly-sorted. In the tracer test, Darcy velocity was estimated to $4.8\times10^{-6}$ cm/day, effective porosity was 0.175, and longitudinal dispersivity was 0.1 m. According to the slug test, hydraulic conductivities of the test wells were estimated as $2.243\times10^{-2}\sim1.634\times10^{-2}$ cm/sec. These hydrogeologic parameters can be used for efficient remediation design of the petroleum contaminated area.

Concentration Distribution of PBDEs and PCBs in Soil (토양 중 PBDEs와 PCBs의 농도분포)

  • Lee, Sung-Hee;Cho, Ki-Chul;Yeo, Hyun-Gu
    • Journal of Environmental Health Sciences
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    • v.35 no.6
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    • pp.510-516
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    • 2009
  • Polybrominated diphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs) were measured in soil samples of Ansung in Kyonggi-province to investigate concentration distribution of PBDEs and PCBs. The 10 soil samples were collected using a stainless steel hand-held corer that was cleaned before and after each sample using hexane. Total concentration of PBDE and PCBs were 2,205.3 and 348.1 pg/g dry weight (DW) in soil sample, respectively. BDE-209 showed as the most abundant congener in soil samples which was related to imported amount and usage amount of deca-BDE technical mixture in Korea. Also, BDE-99, BDE-47, BDE-100 deposition in soil sample was higher than other congeners and was related to the imported and usage amount reported for penta-BDE technical mixture in Korea. Correlation coefficient between PBDE contribution and technical mixture formulation (Bromokal 70-5DE) were significant (r=0.91, p<0.01) which suggests the influence of sources in this technical mixtures.

Characteristics of Natural Pedo-geochemical Background for Ni, Cu and Zn in Volcanic Soils of Jeju (제주도 화산회토양에서 Ni, Cu 및 Zn의 자연함유량 특성)

  • Lim, Han-Cheol;Moon, Kyung-Hwan;Jeon, Seung-Jong;Chang, Kong-Man;Hyun, Hae-Nam
    • Korean Journal of Soil Science and Fertilizer
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    • v.41 no.3
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    • pp.199-205
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    • 2008
  • This study was carried out to find out causes of high Ni content and characteristics of potential increase of Cu and Zn contents in the soils of Jeju area. Soil samples were collected from 4 series of Alfisols, 35 series of Andisols, 9 series of Entisols, 16 series of Inceptisols, and 1 series of Ultisols, respectively. Parent material (gravel) samples were also collected from the same sites where soil samples were taken. Both soil and parent material samples were analyzed for Ni, Cu, and Zn. The average Ni content of all the soil samples was $79.2mg\;kg^{-1}$, which was exceeded warning standard for Ni against the Soil Conservation Act in Korea, whereas that of Cu and Zn contents were lower than the warning standard for Cu and Zn. The difference of Ni, Cu and Zn content was not consistent depending upon soil color. Ni content in the soil samples from Entisols was particularly high, whereas that from the other soil orders was similar to each other. Cu and Zn contents in the soil samples were similar regardless of the difference in soil order. Ni content in Seongsan Formation(SSF), Cinder cone(C) and acicular Feldspar Olivine Basalt(FOB) was high, while that in Feldspar Basalt(FB) and Trachy Andesite lava(TA) was low. Ni content in the non-agricultural land was higher compared with that in the agricultural land, whereas Cu and Zn contents in the non-agricultural land were significantly lower than that of agricultural land. The topsoil/subsoil ratios for Ni content in most of the soil samples were less than 1, while that for total contents of Cu and Zn in many of the soil samples were larger than 1 indicating potential Cu and Zn accumulation in topsoil by artificial reasons.

Analysis of Pathogenic Microorganism's Contamination on Cultivation Environment of Strawberry and Tomato in Korea

  • Oh, Soh-Young;Nam, Ki-Woong;Kim, Won-Il;Lee, Mun Haeng;Yoon, Deok-Hoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.47 no.6
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    • pp.510-517
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    • 2014
  • The purpose of this study was to analyze microbial hazards for cultivation environments and personal hygiene of strawberry and tomato farms at the growth and harvesting stage. Samples were collected from thirty strawberry farms and forty tomato farms located in Korea and tested for Staphylococcus aureus and Bacillus cereus. To investigate the change in the distribution of the S. aureus and B. cereus, a total of 4,284 samples including air born, soil or medium, mulching film, harvest basket, groves and irrigation water etc. were collected from eight strawberry farms and nine tomato farms for one year. As a result, total S. aureus and B. cereus in all samples were detected. Among the total bacteria of strawberry farms, S. aureus (glove: $0{\sim}2.1Log\;CFU/100cm^2$, harvest basket: $0{\sim}3.0Log\;CFU/100cm^2$, soil or culture media: 0~4.1 Log CFU/g, mulching film: $0{\sim}3.8Log\;CFU/100cm^2$), B. cereus (glove: $0{\sim}2.8Log\;CFU/100cm^2$, harvest basket: $0{\sim}4.8Log\;CFU/100cm^2$, soil or culture media: 0~5.3 Log CFU/g, mulching film: $0{\sim}4.5Log\;CFU/100cm^2$) were detected in all samples. The total bacteria of tomato farms, S. aureus (glove: $0{\sim}4.0Log\;CFU/100cm^2$, harvest basket: $0{\sim}5.0Log\;CFU/100cm^2$, soil or culture media: 0~6.1 Log CFU/g, mulching film: $0{\sim}4.0Log\;CFU/100cm^2$), B. cereus (glove: $0{\sim}4.0Log\;CFU/100cm^2$, harvest basket: $0{\sim}4.3Log\;CFU/100cm^2$, soil or culture media: 0~5.9 Log CFU/g, mulching film: $0{\sim}4.7Log\;CFU/100cm^2$) were detected in all samples. The contamination of S. aureus and B. cereus were detected in soil, mulching film and harvest basket from planting until harvest to processing, with the highest count recorded from the soil. But S. aureus and B. cereus were not detected in irrigation water samples. The incidence of S. aureus and B. cereus in hydroponics culture farm were less than those in soil culture. The amount of S. aureus and B. cereus detected in strawberry and tomato farms were less than the minimum amount required to produce a toxin that induces food poisoning. In this way, the degree of contamination of food poisoning bacteria was lower in the production environment of the Korea strawberry and tomato, but problems can be caused by post-harvest management method. These results will be used as fundamental data to create a manual for sanitary agricultural environment management, and post-harvest management should be performed to reduce the contamination of hazardous microorganisms.

Experimental Research on Impulse Breakdown Characteristics of Soil

  • Lee, Jaebok;Sughun Chang;Sungho Myung;Yuengue Cho
    • KIEE International Transactions on Electrophysics and Applications
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    • v.4C no.2
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    • pp.60-63
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    • 2004
  • The electrical breakdown characteristics of different types of soil samples have been measured. It is shown that the threshold soil breakdown strength is affected by many factors, such as types of soil, grain size, and soil compaction. The breakdown process in the test soil samples appears to be due to air ionization in the voids between the soil particles. The results have been compared with the relevant experimental results of other researchers.