• Title/Summary/Keyword: soil medium

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Gametophyte Propagation and Sporophyte Formation of Asian Chain Fern [Woodwardia japonica (L. f.) Sm.] Under Various Medium Conditions In Vitro and Ex Vitro

  • Cho, Ju Sung;Jang, Bo Kook;Park, Kyungtae;Lee, Ha Min;Lee, Cheol Hee
    • Korean Journal of Plant Resources
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    • v.32 no.6
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    • pp.735-742
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    • 2019
  • This study investigated a suitable method that could be applied for Asian chain fern [Woodwardia japonica (L. f.) Sm.] to propagate gametophytes and promote sporophyte formation. The gametophytes used in all experiments were obtained from germinated spores in vitro and were subcultured at 8-week intervals. The most appropriate media for gametophyte propagation was identified by culturing 300 mg of gametophyte in Murashige and Skoog (MS) basal medium (1/8, 1/4, 1/2, 1, 2), and Knop medium for 8 weeks. As a result, fresh weight of the gametophyte was increased by 56.7-fold on MS medium. Moreover, antheridium formation as well as gametophyte growth was improved on MS medium, especially. To improve the sporophyte formation ex vitro, 1.0 g of gametophyte was ground with distilled water and spread on eight combinations onto four different culture mediums, such as bed soil, peat moss, perlite and decomposed granite. Then generation and growth of sporophytes were investigated after cultivation for 10 weeks. As a result of this experiment, peat moss had a promotive effect of sporophyte formation at single-use and mixed culture soils. In particular, a mixture of bed soil, peat moss and perlite in a 1:1:1 ratio (v/v/v) led to the accelerated formation (782.5 ea/pot) and the frond growth of sporophytes. This included increases in length and width of fronds. However, promotive effect of gametophyte growth and sporophyte formation was not found at single-use and treatment with high ratio of bed soil.

Improved Germination and Seedling Growth of Echinochloa crus-galli var. frumentacea in Heavy Metal Contaminated Medium by Inoculation of a multiple-Plant Growth Promoting Rhizobacterium (m-PGPR) (중금속 오염배지에서 식물성장증진 근권미생물에 의한 식용 피 발아율과 유식물 성장 증진)

  • Lee, Ah-Reum;Bae, Bum-Han
    • Journal of Soil and Groundwater Environment
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    • v.16 no.5
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    • pp.9-17
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    • 2011
  • Positive effect of multiple-PGPR (Plan Growth Promoting Rhizobacteria), isolated from heavy metal contaminated soil, on the germination of Barnyard grass (Echinochloa crus-galli var. frumentacea) was quantitatively estimated in 5 heavy metal (Cd, As, Ni, Cu, and Pb) contaminated liquid medium. The $EC_{50}$ value for respective heavy metal was estimated by TSK (Trimmed Speraman-Karber) model based on germination rate. The results showed overall increase in $EC_{50}$ with PGPR inoculation. The $EC_{50}$ value increased 1.4% from 96.0 mg/L (control) to 97.4 mg/L (PGPR-treated) in As contaminated medium. In Ni contaminated medium, the $EC_{50}$ value increased 31.9% from 148.0 mg/L (control) to 195.2 mg/L (PGPR-treated), while the $EC_{50}$ showed 4.8% increase from 63.4 mg/L (control) to 66.5 mg/L (PGPR-treated) in Cu medium. Overall seedling growth was stronger in the PGPR treated seeds than that in the control, but positive effect on seedling growth was not conspicuous. At effective concentration of 100 mg/L, the average seedling length of the PGPR treatment in As, Cd, Cu, and Ni medium, respectively, was 1.13, 0.14, 0.40, and 0.06 cm longer than that in the control. However, the increase of seedling growth was statistically insignificant (p < 0.05). These results suggest that inoculation of the isolated-PGPR exerts positive effects on seed germination by reducing heavy metal toxicity and can be an effective tool for application of phytoremediation on heavy metal contaminated soils.

Dynamic impedance of a floating pile embedded in poro-visco-elastic soils subjected to vertical harmonic loads

  • Cui, Chunyi;Zhang, Shiping;Chapman, David;Meng, Kun
    • Geomechanics and Engineering
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    • v.15 no.2
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    • pp.793-803
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    • 2018
  • Based on the theory of porous media, an interaction system of a floating pile and a saturated soil in cylindrical coordinates subjected to vertical harmonic load is presented in this paper. The surrounding soil is separated into two distinct layers. The upper soil layer above the level of pile base is described as a saturated viscoelastic medium and the lower soil layer is idealized as equivalent spring-dashpot elements with complex stiffness. Considering the cylindrically symmetry and the pile-soil compatibility condition of the interaction system, a frequency-domain analytical solution for dynamic impedance of the floating pile embedded in saturated viscoelastic soil is also derived, and reduced to verify it with existing solutions. An extensive parametric analysis has been conducted to reveal the effects of the impedance of the lower soil base, the interaction coefficient and the damping coefficient of the saturated viscoelastic soil layer on the vertical vibration of the pile-soil interaction system. It is shown that the vertical dynamic impedance of the floating pile significantly depends on the real stiffness of the impedance of the lower soil base, but is less sensitive to its dynamic damping variation; the behavior of the pile in poro-visco-elastic soils is totally different with that in single-phase elastic soils due to the existence of pore liquid; the effect of the interaction coefficient of solid and liquid on the pile-soil system is limited.

Evaluation of Effective Soil Moisture From Natural Soil Surfaces (지표면 토양의 유효 수분함유량 산출에 관한 연구)

  • 오이석
    • Korean Journal of Remote Sensing
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    • v.11 no.3
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    • pp.117-127
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    • 1995
  • In this paper several methods for retriving appropriate values of effective soil moisture contents from natural soil surfaces are introduced and compared each other. The soil medium has usually a nonuniform moisture profile; i.e., relatively dry at the top layer and relatively wet at the bottom layer. The effective soil moisture represents the quantitative value of soil moisture of the inhomogeneous soil medium in an average sense. A simple method is an arithmetic averaging of soil moisture values obtained from several layers of a soil surface. Otherwise, the penetration depths can be computed from a homogeneous and an inhomogeneous soil surfaces and compared in order to obtain the effective soil mosture. The other method is to obtain the effective soil moisture by comparing the reflectivities from both of a homogeneous and an inhomogeneous surfaces. Those methods are compared and the reflectivity technique is examined in more detail since the rader scattering is dominated by the reflectivity instead of the penetration.

Research Trends for Soil-Related Algal Toxicity (토양 관련 조류독성 연구동향)

  • Nam, Sun-Hwa;An, Youn-Joo
    • Journal of Korean Society of Environmental Engineers
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    • v.35 no.8
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    • pp.607-612
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    • 2013
  • Soil ecological risk assessment requires terrestrial toxicity data based on trophic levels including plants, earthworms, nematodes, and springtails. To expand the trophic levels, it is needed to consider primary producer algae, nearly distributed in terrestrial environment, as representative terrestrial test species. In this study, we collected research cases focused on soil-related test species and exposure media from SCI papers, and analyzed exposure media, test species, test chemicals, and other test methods, for reviewing research trends of soil-related algal toxicity. Up to now, in the soil-related algal toxicity, test species were 8 cases (Pseudokirchneriella subcapitata, Chlorella vulgaris, Scenedesmus bijugatus, Chlorococcum infusionum, Scenedesmus subspicatus, Nostoc linckia, Synechococcus elongatus, and Chlorococcum sp.) and endpoints were cell count or photosynthetic pigment content. Also, 5 of exposure media were liquid medium, soil extracts, porewater, agar medium, and soil. Most of papers used algae isolated from natural soils or soil extracts. There were only one case for assessing algal toxicity in soil medium. More researches regarding algal toxicity in soil environments need to be conducted consistently.

A Study on Plant Effects on Decontamination of Polluted Indoor Air with Formaldehyde (포름알데히드로 오염된 실내공기의 정화에 미치는 식물효과에 관한 연구)

  • Park, Soyoung;Kim, Jeoung;Jang, Young-Kee;Sung, Kijune
    • Journal of Environmental Impact Assessment
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    • v.14 no.4
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    • pp.147-155
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    • 2005
  • This study was designed to investigate the effect of plant as a botanical air purification on the indoor pollution by formaldehyde. Three indoor plants such as Dracaena marhginata, Spathiphyllum and Dracaena reflexa, were placed in the artificially contaminated reactor under laboratory condition. Both plant and soil effects on removal of formaldehyde from contaminated indoor air were observed. Reductions in the formaldehyde levels appeared to have been associated with soil medium factors as well as plant factors. The effect of soil on formaldehyde reduction was high in the early stage of the experiment and the results suggest that sorption could be more important factor than microbial degradation in the initial dissipation of contaminants in the soil. It was suggested that the effect of plant on formaldehyde reduction might be related to the plant species, total leaf surface area of plant, degree of contribution of soil medium, and exposed concentration level. The results of this study showed that air purification using plants is an effective means of reduction on indoor formaldehyde level, though, utilization of soil media with high sorption capacity and/or supplementary purifying aids were also suggested when the source is continuous or exposed concentration level is high.

Assessment of effect of material properties on seismic response of a cantilever wall

  • Cakir, Tufan
    • Geomechanics and Engineering
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    • v.13 no.4
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    • pp.601-619
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    • 2017
  • Cantilever retaining wall movements generally depend on the intensity and duration of ground motion, the response of the soil underlying the wall, the response of the backfill, the structural rigidity, and soil-structure interaction (SSI). This paper investigates the effect of material properties on seismic response of backfill-cantilever retaining wall-soil/foundation interaction system considering SSI. The material properties varied include the modulus of elasticity, Poisson's ratio, and mass density of the wall material. A series of nonlinear time history analyses with variation of material properties of the cantilever retaining wall are carried out by using the suggested finite element model (FEM). The backfill and foundation soil are modelled as an elastoplastic medium obeying the Drucker-Prager yield criterion, and the backfill-wall interface behavior is taken into consideration by using interface elements between the wall and soil to allow for de-bonding. The viscous boundary model is used in three dimensions to consider radiational effect of the seismic waves through the soil medium. In the seismic analyses, North-South component of the ground motion recorded during August 17, 1999 Kocaeli Earthquake in Yarimca station is used. Dynamic equations of motions are solved by using Newmark's direct step-by-step integration method. The response quantities incorporate the lateral displacements of the wall relative to the moving base and the stresses in the wall in all directions. The results show that while the modulus of elasticity has a considerable effect on seismic behavior of cantilever retaining wall, the Poisson's ratio and mass density of the wall material have negligible effects on seismic response.

골프장 농약 검사를 위한 다성분동시시험방법 확립에 관한 연구

  • 이민효;노회정;박종겸;윤정기;김찬섭
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2002.09a
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    • pp.81-85
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    • 2002
  • The possibility of simultaneous analysis of 24 pesticides out of 30 residual pesticides which are subjected to test in the golf courses was examined. The utility of the simultaneous analysis of multi-residue pesticides was evaluated by recovery test through a standard addition method of pesticides in water, soil, and lawn grass. The experimental results of the recovery rates for individual pesticides are as follows : The number of pesticide of which average recovery was over 70% regardless of medium was 16 pesticides. These pesticides were composed of 8 organophosphorus pesticides(Chlorpyrifos, Chlorpyrifos-methyl, Diazinon, EPN, Fenitrothion, Phenthoate, Phosalone, and Toclofos-methyl). 4 organochlorinated pesticides(Daconil, Captan, Endosulfan, and Tetradifon), 2 pyrethroid pesticides(Fepropathrin, Lambda-cyhalothrin) and 2 other pesticides(Bromopropylate, Pendimethalin). On the other hand, in case of Dicofol, average recovery by medium was over 70% for water and lawn grass but was only 53.3% for soil. Therefore, the simultaneous analytical method applied in this experiment is not appropriate for analysis of Dicofol in soil. Furthermore, among 7 pesticides, 2 pesticides(Amitraz and Pyraclofos) showed that theirs average recovery rates deviated from criteria(70~130%) at almost all media, while 5 pesticides(Bensulide, Deltamethrin, Iprodione, Phosphamidon and Tralomethlin) were not detected from all media by selected GC detector(NPD or ECD).

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Performance of Zoysia spp. and Axonopus compressus Turf on Turf-Paver Complex under Simulated Traffic

  • Chin, Siew-Wai;Ow, Lai-Fern
    • Weed & Turfgrass Science
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    • v.5 no.2
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    • pp.88-94
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    • 2016
  • Vehicular traffic on turf results in loss of green cover due to direct tearing of shoots and indirect long-term soil compaction. Protection of turfgrass crowns from wear could increase the ability of turf to recover from heavy traffic. Plastic turfpavers have been installed in trafficked areas to reduce soil compaction and to protect turfgrass crowns from wear. The objectives of this study were to evaluate traffic performance of turfgrasses (Zoysia matrella and Axonopus compressus) and soil mixture (high, medium and low sand mix) combinations on turf-paver complex. The traffic performance of turf and recovery was evaluated based on percent green cover determined by digital image analysis and spectral reflectance responses by NDVI-meter. Bulk density cores indicated significant increase in soil compaction from medium and low sand mixtures compared to high sand mixture. Higher reduction of percent green cover was observed from A. compressus (30-40%) than Z. matrella (10-20%) across soil mixtures. Both turf species displayed higher wear tolerance when established on higher sand (>50% sand) than low sand mixture. Positive turf recovery was also supported by complementary spectral responses. Establishment of Zoysia matrella turf on turfpaver complex using high sand mixture will result in improved wear tolerance.