• 한국식품과학회지
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• 제26권5호
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• pp.512-519
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• 1994

박하를 대상으로 재배조건 및 수확시기에 빠른 방향 성분의 함량변화를 조사한 결과 박하의 생육은 비닐보온시험구에서 초기에 왕성한 생육을 보였지만, 수확기가 가까와 짐에 따라 다른 시험구와 유사하게 성장하였으며, 강유량과 일조시간에 따라 줄기와 잎의 성장속도에서 매우 많은 차이를 보였다. 수확시기에 따른 조성분의 변화는 l-menthone의 l-menthol로의 환원과정이 일조시간의 차이에 따라 그 진행속도가 빨라졌으며, 수확말기에 가서는 특히 menthofuran의 함량이 급속히 증가하는 현상을 보였다. 따라서 전체적으로 정유 조성분의 비율이 가장 양호한 시험구는 7월 5일과 7월 7일 수확분으로서, 이 시기가 수확 적기임을 알 수 있었고 정유의 우율도 이 시기에 가장 높게 확인되었다.

• Korean Chemical Engineering Research
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• 제46권4호
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• pp.819-823
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• 2008

유류 오염 토양으로부터 항공유 JP-8을 분해하는 능력이 우수한 균주를 분리하여 16S rDNA 염기서열 분석한 결과 Rhodococcus fascians로 확인되었다. 분리된 R. fascians를 MSM 배지에 JP-8을 탄소원으로 배양하여 그 성장특성과 JP-8 분해 특성을 고찰하였다. 분리된 R. fascians는 2% JP-8을 포함하는 배지에서 5일 이내에 JP-8 대부분을 분해하였다. 16% JP-8을 포함하는 배지에서는 10일 동안 배양액 내 JP-8의 농도를 초기 농도의 14%까지 낮출 수 있었다. $17^{\circ}C$ 부터 $32^{\circ}C$ 까지 JP-8의 분해에 미치는 영향을 살펴본 결과 5일 후에는 모든 시료에서 JP-8의 대부분이 제거되었으며 온도가 낮을수록 R. fascians에 의하여 분해되는 JP-8의 양이 많은 것으로 나타났다. 배지의 초기 pH는 pH5에서 초기 분해속도가 비교적 낮았으나 pH8 까지 모두 5일만에 JP-8의 대부분이 제거되었다.

• KSBB Journal
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• 제11권6호
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• pp.654-662
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• 1996

원유에 대한 분해능이 있는 균주를 분리하고, 이들 균주의 균체생육과 유화활성 및 원유 전환율을 검토하여 4균주 즉, A54, H17, H17-1 그리고 F6 를 선멸하였다. 이들 균주중 H17-1 균주를 최종선멸하여 형태학적 빛 생화학적 그리고 생리학적 특성 을 조사한 후 Nocardia sp. H 17-1로 명명하였다. Nocardia sp. H17-1의 배양 시간에 따른 균체생육, 유화도 그리고 원유 전환율을 측정한 결과, 균체수 는 $9.1\times109 CFU/mL$었고, 유화활성과 원유 전환율 은 각각 480 unit/mL와 약 83%로서 최대치에 도달하였다. 또한, 다양한 탄화수소를 탄소원으로 이용 하였다. 원유분해를 위한 배양조건 및 환경인자의 영향을 조사한 결과 배양온도는 $^30{\circ}C$, 초발 pH는 7.0, 염분농도는 2.0%이며, 5% 이상에서는 유화활 성이 현저히 감소하였다. 원유농도는 3%, 무기염류 의 농도는 12.5 mM $NH_4N0_3$, 0.057 mM $K_2HPO_4$로 나타났다. 또한, 잔류 원유의 GC 분석 결과 CI6(n­ hexadecane) 이상의 n-alkane peak가 현저히 감소하였다.

• 산업경영시스템학회지
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• 제20권43호
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• pp.211-218
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• 1997

Since Industrial Revolution, the sudden industrialization and population explosion have brought about a serious environmental pollution on the air of the earth, the quality of water and the soil, and the greenhouse effect by the destruction of the ozone layer and the greenhouse gas, the organism diversity decline, the desertification by the ruining of woods and the overall global environmental pollution such as the contamination of the ocean and rivers have been come to the front. So the new recognition on the environment is being spreaded in construction business and the efforts for certification acquisition of ISO 14000 being followed by ISO 9000 are progressing, Accordingly, with an example of H Company where integration system is built so that it can minimize the efforts and cost at the construction of the management system, operation and management after the fact, this paper shall suggest the practical scheme of 3 steps through a study to integrate both existing quality management system and new environmental management system. As the integration system of the quality and environment which is constructed by this method is executed, the number of the established and managed document is cut by about a third and so the disorder to encounter between these documents is prevented and unnecessary efforts is reduced in constructing a system, consequently the efficiency of the system is accomplished. Also as the dualization of quality management system and environmental management system additionally needs more men and money after certification acquisition, the non-construction of the integration system may give rise to a cause to aggravate the burden of the company and hereafter when a new system is imported, it may be very difficult to integrate the system. This system integration enables a efficient and effective system to be constructed , operated , and managed after the fact.

• 한국잡초학회지
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• 제14권2호
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• pp.156-162
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• 1994

수수 줄기에 함유된 타감물질(他感物質)중 가장 강한 활성을 나타내는 물질을 rapid chromatography, flash flow column chromatography로 분리하고 thin layer chromatography와 HPLC로 정제하여 GC-MS로 분석하였다. Butanol, acetic acid 및 water의 용매 조합을 달리하여 타감물질을 분리한 결과 butanol (8) : actic acid (1) : water (1) 분획에서 억제효과가 가장 크게 나타났으며, flash flow column chronatography와 TLC로 분리 한 결과 가장 활성을 나타내는 물질은 Rf 0.71에서 나타났으며 HPLC로 순수분리한 결과 Rt 20.40min에서 elution되었다. 이 물질은 주황색을 띄며 methanol에 용해성이 있었다. 정제된 물질을 GC-MS로 분석한 결과 예상되는 물질은 1-methyl-1-(2-propinonyl)-hydrazine, 1-aziridineethanol, 5-chloro-2-pentanone, 2-(methylseleno)-ethanamine 중한 물질일 것으로 추측되었다.

• 한국대기환경학회지
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• 제27권4호
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• pp.395-404
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• 2011

Asian dust evens took place in Seoul on 27~28 March and 31 March~1 April 2007, during which the mass and chemical compositions of $PM_{10}$ were measured at urban area in Seoul, Korea. In conjunction with $PM_{10}$ compositions, the behaviors of gas precursors such as CO, $O_3$, $SO_2$, and $NO_2$ and meteorological parameters and air mass trajectories were thoroughly examined. The earlier case was a weak dust incidence which was characterized by elevated concentrations of CO, $SO_2$ and $NO_2$ as well as secondary aerosols. In contrast, the later showed the trait of the dust aerosols associated with high $PM_{10}$ mass and $Ca^{2+}$ concentrations. In general, the fractions of ionic species against mass decreased with increase in dust loading. The ratios of ${SO_4}^{2-}$ to ${NO_3}^-$ and $SO_2$ to $NO_2$ were similar in temporal variations, suggesting the concentrations of secondary aerosols were sensitive to the level of precursor gases. In this study, $Na^+$ and $Cl^-$ were also highly elevated during the heavy dust episode, which is thought to have originated from alkaline soils spreading through the northeast regions of China.

• 산업식품공학
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• 제14권3호
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• pp.263-270
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• 2010

Xylanase를 생산하는 호알칼리성 균주를 토양으로부터 분리하여 동정하였다. 토양으로부터 분리한 호알칼리성 균주 3000여종 중 xylanase 활성이 가장 높은 균주인 strain DK-2386 균주를 선별하였다. Gram 염색과 SEM을 통한 형태학적 특성을 관찰하였으며, Methods for General and Molecular Bacteriology와 Bergey's manual of systematic bacteriology, 그리고 Biochemical tests for identification of medical bacteria에 준하여 생화학적 특성을 확인한 결과 배양액의 색은 붉은 빛을 나타내었으며, Gram양성 간균, 내생포자형성, catalase 양성, oxidase 양성, 혐기성 양성, glucose로부터 gas 형성 양성, casein 가수분해 양성, starch 가수분해 양성, CMC 가수분해 양성, xylan 가수분해 양성, nitrate 환원능 양성이며 10% NaCl 농도에서도 생육하는 것을 확인하였다. 균체의 지방산 조성 분석 결과 $C_{15:0\;ISO}$ 27.35%와 $C_{15:0\;ANTEISO}$ 31.54%, $C_{16:0}$ 9.70%로 이루어져 Bacillus속으로 동정되었으며, 16S rDNA 염기서열 분석결과 B. agaradhaerens와 100%의 유사성을 갖는 것을 확인하였다. (사)한국종균협회로부터 분양받은 Bacillus agaradhaerens 40952, B. alcalophilus와 strain DK-2386 균주사이의 생화학적 특성을 비교한 결과 B. agaradhaerens와 속과 종이 같으나 미세하게 특성이 다른 균주로 판단되어 B. agarad haerens DK-2386이라 명명하였다.

• Journal of Forest and Environmental Science
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• 제35권3호
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• pp.150-158
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• 2019

Exotic conifer trees have been extensively planted in southern China because of their high apparent growth and yield. These fast-growing plantations are expected to persist as a considerable potential for temporary and long-term carbon sink to offset greenhouse gas emissions. However, information on the carbon storage across different age ranges in exotic pine plantations is often lacking. We first estimated the ecosystem carbon storage across different age ranges of exotic pine plantations in China by quantifying above- and below-ground ecosystem carbon pools. The carbon storage of each tree component of exotic pine (Pinus elliottii) increased significantly with increasing age in Duchang and Yiyang areas. The stem carbon storage except <10 years in Ji'an areas was the largest component among all other components, which accounts for about 50% of the total carbon storage followed by roots (~28%), branches (~18%), and foliage (~9%). The mean total tree carbon storage of slash pine plantations for <10, 10-20 and 20-30 years across three study areas was 3.69, 13.91 and $20.57Mg\;ha^{-1}$, respectively. The carbon stocks in understory and forest floor were age-independent. Total tree and soil were two dominant carbon pools in slash pine plantations at all age sequences. The carbon contribution of aboveground ecosystem increased with increasing age, while that of belowground ecosystem declined. The mean total ecosystem carbon storage of slash pine plantations for <10, 10-20 and 20-30 years across China was 30.26, 98.66 and $98.89Mg\;ha^{-1}$, respectively. Although subtropical climate in China was suitable for slash pine growth, the mean total carbon stocks in slash pine plantations at all age sequences from China were lower than that values reported in American slash pine plantations.

• Journal of Microbiology and Biotechnology
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• 제31권7호
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• pp.967-977
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• 2021

A total of 37 bacterial isolates were obtained from dye-contaminated soil samples at a textile processing factory in Nakhon Ratchasima Province, Thailand, and the potential of the isolates to decolorize and biotransform azo dye Reactive Red 141 (RR141) was investigated. The most potent bacterium was identified as Paenibacillus terrigena KKW2-005, which showed the ability to decolorize 96.45% of RR141 (50 mg/l) within 20 h under static conditions at pH 8.0 and a broad temperature range of 30-40℃. The biotransformation products were analyzed by using UV-Vis spectrophotometry and Fourier-transform infrared spectroscopy. Gas chromatography-mass spectroscopy analysis revealed four metabolites generated from the reductive biodegradation, namely sodium 3-diazenylnaphthalene-1,5-disulfonate (I), sodium naphthalene-2-sufonate (II), 4-chloro-1,3,5-triazin-2-amine (III) and N¹-(1,3,5-triazin-2-yl) benzene-1,4-diamine (IV). Decolorization intermediates reduced phytotoxicity as compared with the untreated dye. However, they had phytotoxicity when compared with control, probably due to naphthalene and triazine derivatives. Moreover, genotoxicity testing by high annealing temperature-random amplified polymorphic DNA technique exhibited different DNA polymorphism bands in seedlings exposed to the metabolites. They compared to the bands found in seedlings subjected to the untreated dye or distilled water. The data from this study provide evidence that the biodegradation of Reactive Red 141 by P. terrigena KKW2-005 was genotoxic to the DNA seedlings.

• Mycobiology
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• 제49권1호
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• pp.78-85
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• 2021

Morels (Morchella spp.) are valuable medicinal and edible mushrooms. In this study, chemical profiles of aromas and lipophilic extracts of black morel (Morchella importuna) grown in China were analyzed by gas chromatography/mass spectroscopy, along with the evaluation of antioxidant and antimicrobial activities for the lipophilic extracts. Sixty-five compounds in total were identified from the aromas, and 1-octen-3-ol was the main component for aromas of fresh (34.40%) and freeze-dried (68.61%) black morels, while the most abundant compound for the aroma of the oven-dried sample was 2(5H)-furanone (13.95%). From the lipophilic extracts, 29 compounds were identified with linoleic acid as the main compound for fresh (77.37%) and freeze-dried (56.46%) black morels and steroids (92.41%) as the main constituent for an oven-dried sample. All three lipophilic extracts showed moderate antioxidant activities against 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) with the IC50 values ranging 7.56~17.52 mg/mL and 5.75~9.73 mg/mL, respectively, and no obvious antimicrobial activity was observed for lipophilic extracts. The drying methods affect the chemical profile of black morel, and freeze-drying was favorable for retaining nutrients and morel smell. This is the first report on the aroma and lipophilic extracts of M. importuna grown in China.