• 제목/요약/키워드: soil bacterium

검색결과 375건 처리시간 0.022초

Identification of Adenosine Deaminase Inhibitor-producing Bacterium Isolated from Soil

  • SHIN, YONG KOOK;YONG-HA PARK;JAE-DONG LEE;HONG-KI JUN
    • Journal of Microbiology and Biotechnology
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    • 제7권1호
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    • pp.32-36
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    • 1997
  • An adenosine deaminase inhibitor-producing bacterium was isolated from soil. An isolate exhibiting high adenosine deaminase inhibitory activity, was designated J-89, and classified as a strain of Bacillus subtilis on the basis of its morphological, phenotypic characteristics, the menaquinone content and cellular fatty acid composition. To confirm the taxonomic position of the strain we need more information such as DNA-DNA homology and other chemotaxonomic characteristics. In this paper we provisionally named strain J-89 as Bacillus sp. J-89 pending further chemotaxonomic study and analysis of adenosine deaminase inhibitor.

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Degradation of Phenanthrene by Sphingomonas sp. 1-21 Isolated from Oil-Contaminated Soil

  • Ryeom, Tai-Kyung;Lee, Il-Gyu;Son, Seung-Yeol;Ahn, Tae-Young
    • Journal of Microbiology and Biotechnology
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    • 제10권5호
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    • pp.724-727
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    • 2000
  • A Phenanthrene-degrading bacterium, Strain 1-21 was isolated from oil-contaminated soil. This strain was a Gram-negative, aerobic, and rod-shaped bacterium, and exhibited a 99% sequence similarity of 16S rDNA to that of Sphingomonas subarctica. The major cellular fatty acid was a summed feature 7(18:1 w7c, 18:1 w9t, 18:1 s12t), which is a characteristic of the Sphingomonas species. When 200 and 1,000 ppm of phenanthrene was added as the sole carbon source, Strain 1-21 degraded 98% and 67% after 10 days of incubation, respectively. Futhermore, this strain was also able to utilized naphthalene and fluorene as sole carbon and energy sources.

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세포외 Cytosine Deaminase 생산균의 분리 및 동정 (Isolation and Identification of Bacterium Producing Extracellular Cytosine deaminase)

  • 유대식;김대현
    • 한국미생물·생명공학회지
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    • 제25권1호
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    • pp.9-14
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    • 1997
  • A bacterium, strain YK 391 producing extracellular cytosine deaminase, has been isolated from soil sample collected near Taegu City and identified. The strain YK 391 was observed to be a motile Gram-negative rod, and did not produced capsule nor spore. The bacterium produced acid from glucose and trehalose, not from arabinose. Esculine was nto hydrolyzed. The isolate could grow anaerobically at 37$\circ $C, but not at 4$\circ $C. Palmitoleic and palmitic acids comprised over 80% of the fatty acid composition of the strain. The strain. The strain YK 391 was identified as Chromobacterium violaceum YK 391 based on its morphological and physiolohical characteristics, and on the fatty acid composition. The extracellular cytosine deaminase produced by Chromobacterium violaceum YK 391 is believed to be unique because it was active not only on cytosine and 5-fluorocytosine but also on cytidine.

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생물방제균 Pseduomonas fluorescens 2112의 선발과 고추역병균에 대한 항진균성 길항작용 (Selection and Antifungal Activity of Antagonistic Bacterium Pseudomonas sp. 2112 against Red-Pepper Rotting Phytophthora capsici)

  • 이은탁;김상달
    • 한국미생물·생명공학회지
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    • 제28권6호
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    • pp.334-340
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    • 2000
  • In order to select multifunctional powerful antagonistic biocontrol agent against red-pepper rotting fungi Phytophthora capsici, we isolated an indigenous antagonistic bacterium which produces antifungal substances and siderophores from a local soil of Kyongju, Korea. The isolated strain was identified as Pseudomonas fluorescens biotype F. The antibiotic produced from P. fluorescens 2112 inhibited hyphae growth and the zoospore germination of Phytophthora capsici. The favorable carbon, nitrogen source and salts for the production of antibiotic from P. fluorescens 2112 were glycerol, beef extract and LiCi at 1.0%, 0.5% and 5 mM, respectively. And antagonistic activity of P. fluorescens 2112 was confirmed against P. capsici in vivo.

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Cohnella panacarvi sp. nov., a Xylanolytic Bacterium Isolated from Ginseng Cultivating Soil

  • Yoon, Min-Ho;Ten, Leonid N.;Im, Wan-Taek
    • Journal of Microbiology and Biotechnology
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    • 제17권6호
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    • pp.913-918
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    • 2007
  • A Gram-positive, aerobic, rod-shaped, nonmotile, endospore-forming bacterium, designated Gsoil $349^T$, was isolated from soil of a ginseng field and characterized using a polyphasic approach. Comparative analysis of 16S rRNA gene sequences revealed that the strain Gsoil $349^T$ belongs to the family Paenibacillaceae, and the sequence showed closest similarity with Cohnella thermotolerans DSM $17683^T$ (94.1%) and Cohnella hongkongensis DSM $17642^T$ (93.6%). The strain showed less than 91.3% 16S rRNA gene sequence similarity with Paenibacillus species. In addition, the presence of MK-7 as the major menaquinone and $anteiso-C_{15:0},\;iso-C_{16:0},\;and\;C_{16:0}$ as major fatty acids suggested its affiliation to the genus Cohnella. The G+C content of the genomic DNA was 53.4 mol%. On the basis of its phenotypic characteristics and phylogenetic distinctiveness, strain Gsoil $349^T$ should be treated as a novel species within the genus Cohnella for which the name Cohnella panacarvi sp. nov. is proposed. The type strain is Gsoil $349^T\;(=KCTC\;13060^T=\;DSM\;18696^T)$.

감마선 조사된 토양에서 분리된 박테리아 Paenibacillus swuensis DY6T의 완전한 게놈 서열 (Complete genome sequence of Paenibacillus swuensis DY6T, a bacterium isolated from gamma-ray irradiated soil)

  • 김명겸;이승열;정희영;스리니바산 사티야라지
    • 미생물학회지
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    • 제52권4호
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    • pp.500-502
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    • 2016
  • 박테리아 종들은 정교한 효소 시스템의 존재로 인해 이온화 방사선 처리 후에 생존할 수 있는 것으로 보고되어 왔고 몇몇 내생 포자를 생산하는 박테리아 또한 두꺼운 포자껍질의 존재 때문에 방사선에 저항할 수 있다. 이 연구에서는 방사선이 조사된 토양의 시료에서 추출된 Paenibacillus swuensis $DY6^T$의 완전한 게놈 서열을 분석하였다. 이 게놈은 G+C 함량이 49.93%인 5,012,599 bp으로 구성되어 있고 단백질 정보를 암호화한 유전자 4,463개와 133개 RNA 유전자를 포함하고 있다.

Biolog Ecoplate와 DGGE 방법을 이용한 알칼리화 토양의 미생물군집 변화 평가 (Assessment of the Changes in the Microbial Community in Alkaline Soils using Biolog Ecoplate and DGGE)

  • 이은영;홍선화
    • KSBB Journal
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    • 제28권5호
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    • pp.275-281
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    • 2013
  • Soil microbial community analysis of farmland soil sprayed with lye in order to use fertilizer in Nigeria was performed. As a control, two kinds of soils not sprayed with lye, located in Eungo and Lagos with general practice in agriculture was selected. Soil sprayed with lye was pH 8.25 through alkalization reaction, while the other soil samples were pH 6.22 and 5.94. Substrate utilization and species diversity index of soil sprayed with lye were low than that of the other soils with the analysis of Biolog ecoplate. As a result of principal component analysis, the relationship between three samples was low. Microbial community analysis was performed by DGGE and most of them were soil uncultured bacterium. Especially, Uncultured Acidobacteria and Uncultured Methylocystis sp., which had been isolated from the rhizosphere of soybean grown in that site were discovered in the soil sprayed with lye.

Reticulitermes speratus kyushuenesis Morimoto의 장내세균 Bacillus amyloliquefaciens에 의한 Cellulose 분해 능력 (Cellulolytic ability of Bacillus amyloliquefaciens in the gut of Reticulitermes speratus kyushuenesis Morimoto)

  • 박현철;배태웅
    • 한국토양동물학회지
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    • 제3권2호
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    • pp.51-57
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    • 1998
  • A new rod-shaped endospore-forming bacterium isolated from the hindgut flora of the termite, Reticulitermes speratus kyushuenesis Morimoto is described. The isolate stained Gram positive, but the KOH test and the test for L-alanine aminopeptidase were negative. The length of a single cell varies from 2.5-9.0 $\mu $m, and the cell is about 0.5-0.7$\mu $m thick. The isolate had a high cellulolytic ability and was identified as Bacillus amyloliquefaciens.

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흰개미 (Reticulitermes Speratus kyushuenesis Morimoto)와 장내 미생물과의 공생관계 (Symbiotic relationship between termite, Reticulitermes Speratus kyushuenesis Morimoto, and its intestinal microorganisms)

  • 박현철
    • 한국토양동물학회지
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    • 제3권1호
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    • pp.19-24
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    • 1998
  • The importance of the gut microorganism in the termite, Reticulitermes Speratus kyushuenesis Morimoto, was estimated by feeding with several antibiotics. Antibiotics which killed the bacterium, but not the fungi (Ampicillin, Kanamycin, Erythromycin), or killed both the microorganismw (Cephaloridine, Tetracycline) reduced the life span of the termite from 245 days to about 15 days. On the other hand, some antibiotics, penicillin and pimaricin, did not greatly reduce the life span of the termite.

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