• Title/Summary/Keyword: sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE)

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Chemical and Sensory Properties of New Gluten-free Food Products: Rice and Corn Tarhana

  • Yalcin, Erkan;Celik, Sueda;Koksel, Hamit
    • Food Science and Biotechnology
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    • v.17 no.4
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    • pp.728-733
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    • 2008
  • New gluten-free food product (tarhana) was produced using rice and com flours. Chemical and sensory properties of the tarhana samples were investigated and compared with those of traditional wheat tarhana. Generally, sensory analysis results indicated that utilization of com and rice flours in tarhana resulted in acceptable soup properties in terms of some of the sensory properties. The changes in electrophoretic properties of the proteins of the tarhana samples were also studied during the tarhana production. Sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE) results showed that relative intensities of some of the protein bands in tarhana samples generally decreased during fermentation. The decrease was more obvious at the larger molecular weight region. Corn and rice tarhana seem to be promising food products for the celiac patients who have limited choice of cereal based foods.

Characteristics of Bacteriocin Produced by Lactococcus lactis ET45 Isolated from Kimchi (김치에서 분리한 Lactococcus lactis ET45가 생산하는 박테리오신의 특성)

  • Jeong, Seong-Yeop;Park, Chan-Sun;Choi, Nack-Shick;Yang, Hee-Jong;Kim, Cha-Young;Yoon, Byoung-Dae;Kang, Dae-Ook;Ryu, Yeon-Woo;Kim, Min-Soo
    • Korean Journal of Microbiology
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    • v.47 no.1
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    • pp.74-80
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    • 2011
  • Bacteriocin-producing lactic acid bacterium having antagonistic activity against Bacillus cereus, was isolated from Kimchi. The selected strain was identified as Lactococcus lactis by the Bergey's manual and 16S rDNA analysis, and named as L. lactis ET45. The bacteriocin was stable in the pH range 3.0-11.0. The bacteriocin was active over a wide temperature range from $40^{\circ}C$ to $121^{\circ}C$. Optimal culture condition for producing bacteriocin was obtained by growing the cells on MRS medium at pH 7.5 and $30^{\circ}C$ for 18 h. Antibacterial activity of the bacteriocin was completely disappeared by proteinase K, and this means that bacteriocin is a proteinous substance. The molecular weight of bacteriocin was estimated to be about 4.5 kDa by tricine sodium dodecyl sulfate polyacryamide gel electrophoresis (TSDS-PAGE).