• Molecules and Cells
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• v.37 no.7
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• pp.540-546
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• 2014

Several types of genetic and epigenetic regulation have been implicated in the development of drug resistance, one significant challenge for cancer therapy. Although changes in the expression of non-coding RNA are also responsible for drug resistance, the specific identities and roles of them remain to be elucidated. Long non-coding RNAs (lncRNAs) are a type of ncRNA (> 200 nt) that influence the regulation of gene expression in various ways. In this study, we aimed to identify differentially expressed lncRNAs in 5-fluorouracil-resistant colon cancer cells. Using two pairs of 5-FU-resistant cells derived from the human colon cancer cell lines SNU-C4 and SNU-C5, we analyzed the expression of 90 lncRNAs by qPCR-based profiling and found that 19 and 23 lncRNAs were differentially expressed in SNU-C4R and SNU-C5R cells, respectively. We confirmed that snaR and BACE1AS were down-regulated in resistant cells. To further investigate the effects of snaR on cell growth, cell viability and cell cycle were analyzed after transfection of siRNAs targeting snaR. Down-regulation of snaR decreased cell death after 5-FU treatment, which indicates that snaR loss decreases in vitro sensitivity to 5-FU. Our results provide an important insight into the involvement of lncRNAs in 5-FU resistance in colon cancer cells.

• Journal of the Korea Convergence Society
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• v.6 no.1
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• pp.77-83
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• 2015

Though the SNA (social network analysis ; SNA) has been used for various fields, esp. social science field, ig. politics, journalism, and science of public administration as well as natural science field, there are few studies about the introduction of analysis tools. In order to perform the SNA, collecting data which are fit for the purpose, statistical values deduction and visualized results made by analysis tool are necessary, but the studies, which explain them systematically, are not sufficient yet. So, in this study, we are intended to introduce the analytic process, from the data input to the interpretation, with proven data. using the R program, which is free, in order to help researchers who have any plan to study using the SNA. The proven data in this study are quoted ones in the domestic scientific journals of food, which are those supplied citation index DB of Korean scientific journals. As a study methodology, the SNA is a new paradigm to substitute existing research methods as well as a complement of statistical analysis. Therefore, this study would contribute to vitalization of the SNA.

• Wind and Structures
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• v.36 no.3
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• pp.215-220
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• 2023

We study the progressive full-scale wind tunnel tests on a high solidity vertical axis wind turbine (VAWT) for various tip speeds and pitch angles to understand the VAWT shaft system's dynamics using 0-1 Test for chaos. We identify that while varying rotor speed (tip speed) of the turbine, the system's dynamics change from periodic to chaotic through quasiperiodic and strange non-chaotic (SNA) states. The present study is the first experimental evidence for the existence of these states in the VAWT shaft system to the best of our knowledge. Using the asymptotic growth value Kc in 0-1 test, when the turbine operates at the low tip speeds and high pitch angles for low incoming wind speeds, the system behaves periodic (Kc ≈ 0). However, when the incoming wind speed increases further the system's dynamics shift from periodic to chaotic vibrations through quasi-periodic and SNA. This phenomenon is due to the dynamic stalling of blades which induces chaotic vibration in the VAWT shaft system. Further, the singular continuous spectrum method validates the presence of SNA and differentiates the SNA from chaotic vibrations.

• Proceedings of the Korea Technology Innovation Society Conference
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• 2017.11a
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• pp.1375-1399
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• 2017

이 연구의 목적은 해양바이오 분야에 지원된 정부 R&D의 지원동향 분석을 기초로 향후 이 분야 정부연구개발 사업에 필요한 함의를 도출하는 데 있다. 이 연구에서는 NTIS DB를 통해 식별한 3,030건의 세부과제를 대상으로 기술통계와 과제명을 기초로 한 주제어 간 연결 관계를 SNA(Social Network Analysis) 방법을 이용해 실증분석 하였다. 이 연구에서 수행한 실증분석 결과를 간략하게 요약하면 다음과 같다. 첫째, 기술통계 분석 결과 최근으로 올수록 해양바이오 분야에 지원된 정부지원 연구개발 사업은 사업수와 연구비 지원규모 면에서 지속적인 성장을 보이고 있다. 둘째, 분야 특성상 이 분야에 대한 R&D 지원은 해수부가 주도적이지만, 대체로 범부처적 성격을 갖는다. 셋째, SNA를 통한 실증분석 결과 대부분의 사업이 해양생명자원이 가진 특이기작으로부터 유용신소재를 확보하기 위한 목적을 갖고 있는 것으로 분석되었다.

• Molecules and Cells
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• v.43 no.3
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• pp.228-235
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• 2020

The Drosophila transmembrane semaphorin Sema-1a mediates forward and reverse signaling that plays an essential role in motor and central nervous system (CNS) axon pathfinding during embryonic neural development. Previous immunohistochemical analysis revealed that Sema-1a is expressed on most commissural and longitudinal axons in the CNS and five motor nerve branches in the peripheral nervous system (PNS). However, Sema-1a-mediated axon guidance function contributes significantly to both intersegmental nerve b (ISNb) and segmental nerve a (SNa), and slightly to ISNd and SNc, but not to ISN motor axon pathfinding. Here, we uncover three cis-regulatory elements (CREs), R34A03, R32H10, and R33F06, that robustly drove reporter expression in a large subset of neurons in the CNS. In the transgenic lines R34A03 and R32H10 reporter expression was consistently observed on both ISNb and SNa nerve branches, whereas in the line R33F06 reporter expression was irregularly detected on ISNb or SNa nerve branches in small subsets of abdominal hemisegments. Through complementation test with a Sema-1a loss-of-function allele, we found that neuronal expression of Sema-1a driven by each of R34A03 and R32H10 restores robustly the CNS and PNS motor axon guidance defects observed in Sema-1a homozygous mutants. However, when wild-type Sema-1a is expressed by R33F06 in Sema-1a mutants, the Sema-1a PNS axon guidance phenotypes are partially rescued while the Sema-1a CNS axon guidance defects are completely rescued. These results suggest that in a redundant manner, the CREs, R34A03, R32H10, and R33F06 govern the Sema-1a expression required for the axon guidance function of Sema-1a during embryonic neural development.

• Journal of Cadastre & Land InformatiX
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• v.49 no.1
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• pp.113-121
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• 2019

The fourth industrial revolution technology focused on the fusion of infrastructure and various advanced technologies related city. Therefore, technical cooperation in various fields of research is essential. In order to activating the fourth industrial revolution technologies, it is necessary to research the state of technology in various fields. Consequently, this paper aims to analysis of domestic and foreign research trends on technology to the fourth industrial revolution using SNA and text mining for web site. We collected text, date data of research paper and report in web site for five years, that is, from January 1st in 2014 to December 31st in 2018. Next, we have deduced the major keywords in public data through analyzing the morphemes. Then we have analyzed the core and related keyword lists through an SNA. In Korea, the focus is on R&D and legal/institutional solution in relation to the fourth industrial revolution technology. On the other hand, in the case of foreign, there was focus on practical technologies for urban services in detail aspects.

• Proceedings of the Korea Technology Innovation Society Conference
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• 2017.05a
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• pp.335-356
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• 2017

본 연구에서는 4차 산업혁명에서 가장 중요하게 대두될 수 있는 융합R&D정책에 대해 소셜 네트워크 분석을 통해 새로운 대안을 제시하려고 한다. 연구장비, 연구성과의 자료들을 활용하여 연구장비 네트워크 분석을 실시하여 융합R&D의 특성 및 구조분석을 실시하며, 연구장비 공동활용 및 다학제 협력연구를 통해 창출될 수 있는 새로운 융합R&D정책에 대한 접근을 시도하려고 한다. 나아가, 연구개발과 사업화 사이에서 더욱 중요한 역할을 할 수 있는 분석과학기술에 대한 정책적 플랫폼 구축관점 대한 시사점을 찾아내려고 한다. 이를 위해 계량적 접근과 비계량적 접근을 동시에 활용하여 연구주제에 대한 타당성을 확보할 것이며, 앞으로 미래산업 및 유망기술에 대한 전략적 선별에 있어 일부 근거를 제시해줄 수 있을 것이다. 이러한 연구결과를 통해 우리나라 기초과학 및 기초과학인프라에 대한 발전적 정책방안과 융합연구 및 융합연구의 사업화에 대한 정책적 관점을 제시하도록 한다. 분석자료는 한국기초과학지원연구원(KBSI) 현황자료를 중심으로 살펴보며, 분석방법은 소셜네트워크분석(SNA:Socail Network Analysis)을 활용한다.

• Journal of Information Technology Applications and Management
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• v.21 no.4
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• pp.65-96
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• 2014

To have global competitiveness in newly growing industry, good quality R&D's in convergence technology arerequired. Korean government also started to emphasize the importance of convergence technology as a new engine of growth for the future development. Since 2008, worldwide energy crisis and concerns on low carbon green growth made people focus on the convergence between information technology (IT) and energy technology (ET). However, the R&D performance comparison among the convergence technologies is not well explored so far. Therefore, this study uses Korea's patent citation database to measure the R&D performance of convergence technologies. We adopt technology development cycle, technology spillover analysis, and technology diffusion analysis to see the knowledge flow from R&D industry to others. We find that IT_ET convergence technology generally shows higher R&D performance than other convergence technologies. Contrary to public belief, convergence R&D by big companies has relatively low performance especially measures in technology spillover and technology diffusion. This implies that they might concentrate on delicate Fuel Cell Energy/Environment Technology (FEIT) or Nano Environment/Energy Information Technology (NEIT) rather than general energy information technologies. We also find that Korea's chemical industry may play a crucial role for the growth of other convergence technologies.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.6
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• pp.3955-3961
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• 2013

Background: Defects of manganese superoxide dismutase (MnSOD) have long been implicated in generation of oxidative stress and risk susceptibility to various cancers. Two functional polymorphisms within the MnSOD gene, including the Val-9Ala of the mitochondrial targeting sequence (MTS) and the Ile58Thr of the exon-3, have been proposed to reduce its enzyme activity and antioxidant potential. Materials and Methods: A high-throughput multiplex SNaPshot$^{(R)}$ system was developed herein for simultaneous analyses of Val-9Ala and Ile58Thr in a single reaction. Genomic DNA extracted from each whole blood sample of 248 patients including 107 with cervical cancer and 141 with breast cancer and from 136 healthy women as controls was analyzed by the multiplex SNaPshot$^{(R)}$ system. Results: The Val/Val, Val/Ala genotypes and the Val allele of the MTS were predominant in patients with cervical or breast cancer as well as healthy women in Thailand. The Ile/Ile genotype and the Ile allele of the exon-3 were found in all of them whereas none of the Ile/Thr, the Thr/Thr genotypes and the Thr allele was detected. Genotypic association of both Val-9Ala and Ile58Thr polymorphisms with cervical cancer and breast cancer of these patients comparing to healthy women was not statistically significant (p<0.05). Conclusions: The Val/Val, Val/Ala genotypes and the Val allele of the MTS were found predominantly but the Ile/Ile genotype and the Ile allele of the exon-3 were detected in patients with cervical cancer, breast cancer and healthy women in Thailand. These two functional polymorphisms (Val-9Ala and Ile58Thr) in MnSOD gene did not associate with susceptibility risk of these cancer patients in Thailand.

• Journal of the Korean Data and Information Science Society
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• v.25 no.6
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• pp.1431-1438
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• 2014

We analyzed the articles from "Kukje Shinmun" and "Busan Ilbo", which are two local newpapers of Busan Metropolitan City. The articles cover from January 1, 2013 to December 31, 2013. Meaningful pattern inherent in 2889 articles of which the title includes "Busan" and "Traffic" and related data was analyzed. Textmining method, which is a part of datamining, was used for the social network analysis (SNA). HDFS and MapReduce (from Hadoop ecosystem), which is open-source framework based on JAVA, were used with Linux environment (Uubntu-12.04LTS) for the construction of unstructured data and the storage, process and the analysis of big data. We implemented new algorithm that shows better visualization compared with the default one from R package, by providing the color and thickness based on the weight from each node and line connecting the nodes.