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(-)-Epigallocatechin Gallate Inhibits the Pacemaker Activity of Interstitial Cells of Cajal of Mouse Small Intestine

  • Kim, Kweon-Young;Choi, Soo-Jin;Jang, Hyuk-Jin;Zuo, Dong-Chuan;Shahi, Pawan Kumar;Parajuli, Shankar Prasad;Yeum, Cheol-Ho;Yoon, Pyung-Jin;Choi, Seok;Jun, Jae-Yeoul
    • The Korean Journal of Physiology and Pharmacology
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    • v.12 no.3
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    • pp.111-115
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    • 2008
  • The effects of (-)-epigallocatechin gallate (EGCG) on pacemaker activities of cultured interstitial cells of Cajal (ICC) from murine small intestine were investigated using whole-cell patch-clamp technique at $30^{\circ}C$ and $Ca^{2+}$ image analysis. ICC generated spontaneous pacemaker currents at a holding potential of -70 mV. The treatment of ICC with EGCG resulted in a dose-dependent decrease in the frequency and amplitude of pacemaker currents. SQ-22536, an adenylate cyclase inhibitor, and ODQ, a guanylate cyclase inhibitor, did not inhibit the effects of EGCG. EGCG-induced effects on pacemaker currents were not inhibited by glibenclamide, an ATP-sensitive $K^+$ channel blocker and TEA, a $Ca^{2+}$-activated $K^+$ channel blocker. Also, we found that EGCG inhibited the spontaneous $[Ca^{2+}]_i$ oscillations in cultured ICC. In conclusion, EGCG inhibited the pacemaker activity of ICC and reduced $[Ca^{2+}]_i$ oscillations by cAMP-, cGMP-, ATP-sensitive $K^+$ channel-independent manner.

A case report : Iguana iguana with cystic calculus (녹색 이구아나의 방광결석증)

  • ;;Michael Fehr
    • Journal of Veterinary Clinics
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    • v.15 no.2
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    • pp.472-475
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    • 1998
  • A female Green iguana (Iguana iguana) was brought to the small animal clinic in Hannover Veterinary school because of anorexia and convulsion. Two months ago, the owner bought two iguanas, one female and one male and had to take a female to the one of local small animal clinic because she, born in 19931 didn't eat well and showed the tremor of the legs. The veterinarian suspected it to have a calcium deficiency and gave an injection of calcium and vitamin D3. But still during the last two months, the female iguana did only eat some lettuce while the other male ate lettuce, fruits and commercial cat-food ($Whiskas^{\circledR}$, Effem, Verden). It was not possible to evaluate the rations of these different "food-components". They are kept in the terrarium with water container. By palpation of the abdomen, hard thing like stone was palpated in the mid-region. The blood test wasn't carried out because of the difficulties of getting blood and because of her bad condition. It was firmly diagnosed as cystic calculus through radiography. Since the animal is in shock state already, the prognosis after surgery was bad. It was assumed to be too late to expect its surviving with the surgery carried out or without it. Cystotomy was performed in the cranial ventral paramedian incision in order to preserve the large ventral abdominal vein in the linen alba region. After two stay sutures ("holding suture" ) with 5-0 polyglactin 910 ($Vicryl^{\circledR}, Ethicon$) on the bladder, cystotomy was carried out and the stone inside was removed. Then, the bladder was closed in one-layer sutures. The abdomen was closed in simple interrupted suture with same material after the instillation of 10 ml saline solution into the abdominal cavity. The cystic calculus was in a size of $5.8{\times} 5.O{\times} 3.5 cm$. The analysis of the stone was carried out in two ways which are the infrared spectroscopy of the stone and quick analysis by $Merckognost^{\circledR}$(Diagnostica Merck, Merck). In the result of the infrared spectroscopy, the stone wee composed of 10% ammonium urate and 90% dihydrated uric acid. This iguana wasn't able to survive.n't able to survive.

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Problems in the Medical Dispute Medication System and Improvement Plan (의료분쟁조정제도 운영상의 문제점 및 개선방안)

  • Choi, Jang Seop
    • The Korean Society of Law and Medicine
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    • v.15 no.2
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    • pp.91-122
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    • 2014
  • For a variety of reasons, the number of medical disputes is continuously rising. Due to the intrinsic qualities of medical treatments, one would find it more apt to subject medical disputes to general conflict resolution procedures rather than to once-for-all decisions under legal suits. To address the increasing medical disputes with greater professionalism and efficiency, the Medical Disputes Mediation Act was enacted and a medical dispute mediation system put in place, while drawbacks have been blamed to both. The current mediation procedures require the respondent's agreement as a disclosure requirement. A reasonable improvement to this would be to amend the regulation of agreement supposition, or to enforce procedural participation only to public health facilities managed by the national or regional government. Furthermore, small claims cases of 20 million KRW or less in claim may be considered for conciliation-prepositive principle. The concentration on small claim medical disputes is a phenomenon that can be addressed by carrying out maximum authentication commissions or similar measures, one of the solutions by enhancing the public trust in the Korea Medical Dispute Mediation and Arbitration Agency. The proper management of medical authentication teams is one way to address the existing problems in the authentication system. For this, the number of team members shall be increased under more flexible authentication procedures. All indemnity resources for medical accidents of force majeure must be borne by the Government, for it is the body principally responsible for social compensation. Placing this cost on the establisher of the subject medical facility holds the possibility of violating fundamental rights. While the costs for subrogation payment system for damages may be borne by the healthcare facility establisher, a deposit-based system must be created for cases in which the facility shuts down, without holding the responsibility for accident cause. Such change to a deposit-based system will evade the controversies of unconstitutionality, etc.

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THE TASTE COMPOUNDS FERMENTED ACETES CHINENSIS (새우젓의 정미성분에 관한 연구)

  • CHUNG Seung-Yong;LEE Eung-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.9 no.2
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    • pp.79-110
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    • 1976
  • In Korea fermented fish and shellfish have traditionally been favored and consumed as seasonings or further processed for fish sauce. Three major items in production quantity among more than thirty kinds which are presently available in the market are fermented anchovy, oyster and small shrimp. They are usually used as a seasoning mixture of Kimchi in order to provide a distinctive flavor. Fermented small shrimp, Acetes chinensis is most widely and largely used ana occupies an important position in food industry of this country. But no study on its taste compounds has been reported. This study was attempted to establish the basic data for evaluating taste compounds of fermented small shrimp. The changes of such compounds during fermentation as free amino acids, nucleotides and their related compounds, TMAO, TMA, and betaine were analysed. In addition, change in microflora during the fermentation under the halophilic circumstance was also investigated. The samples were prepared with three different salt contents of 20, 30 and $40\%$ to obtain the proper degree of fermentation at a controlled tempeature of $20{\pm}2^{\circ}C$. The results are summarized as follows: Volatile basic nitrogen increased rapidly until 108 days of fermentation and afterwards it tended to increase slowly. Amino nitrogen also increased rapidly until 43 days of fermentation and then increased slowly. Extract nitrogen increased and marked the maximum value at 72 day fermentation and then decreased slowly. ADP, AMP and IMP tended to degrade rapidly while hypoxanthine increased remarkably at 27 day fermentation but slightly decreased at 72 day fermentation. It is presumed that the characteristic flavor of fermented small shrimp might be attributed to the relatively higher content of hypoxanthine. In the free amino acid composition of fresh small shrimp abundant amino acids were proline, arginine, alanine, glycine, lysine, glutamic acid, leucine, valine and threonine in order. Such amino acids like serine, methionine, isoleucine, phenylalanine, aspartic acid, tyrosine and histidine were poor. In small shrimp extract, proline, arginine, alanine, glycine, lysine and glutamic acid were dominant holding $18.5\%,\;14.6\%,\;10.8\%,\;8.7\%,\;8.1\%\;and\;7.7\%$ of total free amino acids respectively. The total free amino acid nitrogen in fresh small shrimp was $63.9\%$ of its extract nitrogen. The change of free amino acid composition in the extract of small shrimp during fermentation was not observed. Lysine, alanine glutamic acid, proline, glycine and leucine were abundant in both fresh sample and fermented products. The increase of total free amino acids during 72 day fermentation reached approximately more than 2 times as compared with that of fresh sample and then decreased slowly. Fermented small shrimp with $40\%$ of salt was too salty to be commercial quality as the results of organoleptic test showed. It is found that 72 day fermentation with $20\%\;and\;30\%$ of salt gave the most favorable flavor. It is convinced that the characteristic flavor of fermented small shrimp was also attributed to such amino acids as lysine, proline, alanine, glycine and serine known as sweet compounds, as glutamic acid with meaty taste, and as leucine known as bitter taste. The amount of betaine increased during fermentation and reached the maximum at 72 day fermentation and then decreased slowly TMA increased while TMAO decreased during fermentation. The amount of TMAO nitrogen in fermented small shrimp was $200mg\%$ on moisture and salt free base. Betaine and TMAO known as sweet compounds were abundant in fermented small shrimp. It is supposed that these compounds could also play a role as important taste compounds of fermented small shrimp. At the initial stage of fermentation, Achromobacter, Pseudomonas, Micrococcus denitrificans which belong to marine bacteria were isolated. After 40 day fermentation, they disappeared rapidly while Halabacterium, Pediococcus, Sarcian, Micrococcus morrhuae and the yeasts such as Saccharomyces sp. and Torulopsis sp. dominated. It is concluded that the most important taste compounds of fermented small shrimp were amino acids such as lysine, proline, alanine, glycine, serine, glutamic acid, and leucine, betaine, TMAO and hypoxanthine.

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Involvement of D2 Receptor on Dopamine-induced Action in Interstitial Cells of Cajal from Mouse Colonic Intestine

  • Zuoa, Dong Chuan;Shahia, Pawan Kumar;Choia, Seok;Jun, Jae-Yeoul;Park, Jong-Seong
    • Biomedical Science Letters
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    • v.18 no.3
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    • pp.218-226
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    • 2012
  • Dopamine is an enteric neurotransmitter that regulates gastrointestinal motility. This study was done to investigate whether dopamine modulates spontaneous pacemaker activity in cultured interstitial cells of Cajal (ICCs) from mouse using whole cell patch clamp technique, RT-PCR and live $Ca^{2+}$ imaging analysis. ICCs generate pacemaker inward currents at a holding potential of -70 mV and generate pacemaker potentials in current-clamp mode. Dopamine did not change the frequency and amplitude of pacemaker activity in small intestinal ICCs. On the contrary dopamine reduced the frequency and amplitude of pacemaker activity in large intestinal ICCs. RT-PCR analysis revealed that Dopamine2 and 4-receptors are expressed in c-Kit positive ICCs. Dopamine2 and 4 receptor agonists inhibited pacemaker activity in large intestinal ICCs mimicked those of dopamine. Domperidone, dopamine2 receptor antagonist, increased the frequency of pacemaker activity of large intestinal ICCs. In $Ca^{2+}$-imaging, dopamine inhibited spontaneous intracellular $Ca^{2+}$ oscillations of ICCs. These results suggest that dopamine can regulate gastrointestinal motility through modulating pacemaker activity of large intestinal ICCs and dopamine effects on ICCs are mediated by dopamine2 receptor and intracellular $Ca^{2+}$ modulation.

A Joint Effort between Academia and Industry in the Eastern Part of Kagawa Prefecture in Japan

  • Sato, Kazuishi;Okajima, Kunihiko;Nakajima, Ken-Ichiro
    • Journal of Engineering Education Research
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    • v.15 no.4
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    • pp.14-20
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    • 2012
  • The Regional Cooperative Development Center (RCDC) at Tokushima Bunri University was established in 2005. The role of the RCDC is to make a social contribution and to assist in the development of human resources. The RCDC has made contributions to society by organizing meetings to facilitate the exchange of technology, holding public lectures, supporting joint research projects, providing technical assistance for manufacturers, making available the free use of equipment owned by the university and so on. Additionally, the RCDC has put much effort into giving undergraduate students internship opportunities at industries in the eastern part of Kagawa Prefecture (the TOSAN area) in Japan. The RCDC also trained a contracted researcher from a small company about the creation of intellectual property, including how to conduct a patent prior art search, how to prepare a patent specification, etc. The RCDC has worked in partnership with industry, Sanuki City Society of Commerce and Industry and Higashi-Kagawa City Society of Commerce and Industry in order to revitalize local communities.

Buffering Capacity of Dairy Powders and Their Effect on Yoghurt Quality

  • Kim, Miji;Oh, Sejong;Imm, Jee-Young
    • Food Science of Animal Resources
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    • v.38 no.2
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    • pp.273-281
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    • 2018
  • Preheating conditions (low-, medium-, and high heat-) did not significantly affect the buffering capacity (BC) of skim milk powder (SMP), whereas the level of demineralization significantly affected the BC of whey powders (WP). Heat treatment ($85^{\circ}C$ for 30 min) of both SMP and WP (90% demineralized) mixtures (88:12, 76:24, 64:36 and 52:48; SMP:WP) resulted in a reduced BC, and the extent of this reduction increased with the proportion of WP increased in the samples. High-buffering milk prepared by the addition of phosphate salts (40 mM $NaH_2PO_4$ and 60 mM $Na_2HPO_4$) delayed the rate of pH decline during yoghurt fermentation. The high-buffering yoghurt showed a significantly higher water holding capacity (WHC) than that of control yoghurt (p<0.05), as well as a more uniform and interconnected microstructure with small pore sizes than those of control yoghurt. No significant differences were found between high-buffering and control yoghurt regarding the viable bacterial counts of starter. The manipulation BC can potentially improve the quality characteristics of yoghurts, such as WHC and texture.

A Study on the Behavior of Wrinkles in Cup Drawing with Al alloy by FEM (유한요소법에 의한 합금의 용기 성형시 Al 주름의 거동에 관한 연구)

  • Ko D.L.;Jeon C.Y.;Kim J.M.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2005.06a
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    • pp.1240-1243
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    • 2005
  • The wrinkling in the flange and wall of a part is a predominant failure mode in stamping of sheet metal parts. In many cases this wrinkling may be eliminated by appropriate control of the blank holding force(BHF), but BHF affects the draw depth. Gotoh had studied the wrinkles under $20{\mu}$ in height. In general, the height of wrinkles could be limited under $200{\mu}$ practically. Therefore small BHF can be allowed so that the depth of drawing could be increased. This paper represents the variation of the wrinkles of flange in the part of cup drawing by using aluminium alloy A1050 and A5052. This simulation is used by the explicit finite elements code $PAM-STAMP^{TM}$. The computed results are compared with the experimental results to show the validity of the analysis.

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Torrefaction and Hydrothermal Carbonization (HTC) of Dead Leaves

  • Saqib, Najam Ul;Park, Seong-Kyu;Lee, Jai-Young
    • Journal of Soil and Groundwater Environment
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    • v.19 no.5
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    • pp.45-52
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    • 2014
  • Torrefaction and hydrothermal carbonization (HTC) are productive methods to reclaim energy from lignocellulosic biomass. The hydrophobic, homogenized, energy dense and carbon rich solid fuel can be obtain from torrefaction and hydrothermal carbonization. Dead leaves were carbonized in a stainless steel reactor of volume 200 ml with torrefaction ($250-270^{\circ}C$) for 120 minutes and hydrothermal carbonization ($200-250^{\circ}C$) for 30 minutes, with mass yield solid fuel ranging from 57-70% and energy content from 16.81MJ/kg to 22.01 MJ/kg compare to the biomass. The char produced from torrefaction process possess high energy content than hydrothermal carbonization. The highest energy yield of 89.96% was obtained by torrefaction at $250^{\circ}C$. The energy densification ratio fluctuated in between 1.15 to 1.30. On the basis of pore size distribution of the chars, the definition of the International Union of Pure and Applied Chemistry (IUPAC) was used as a classification standard. The pore diameter was ranging within 11.09-19 nm which play important role in water holding capacity in soil. Larger pores can hold water and provide passage for small pores. Therefore, it can be concluded that high pore size char can be obtained my HTC process and high energy content char of 22.01 MJ/Kg with 34.04% increase in energy can be obtain by torrefaction process.

Study on the Effect of Austenite Grain Size and Mn Content on Hardenability in Boron-added Low Carbon alloys Steels (보론 첨가 저탄소합금강에서 Mn함량과 오스테나이트 결정입도가 경화능에 미치는 영향에 관한 연구)

  • Huh, U.Y.;Rho, Y.S.;Choi, M.S.;Kim, Y.H.;Lee, S.Y.
    • Journal of the Korean Society for Heat Treatment
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    • v.3 no.4
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    • pp.23-40
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    • 1990
  • This study has been carried out to investigate into some effects of Mn content with varying amounts and austenite grain size on hardenability in boron-added Fe-C-Cr-Mo alloy systems. (1) Austenite grains have been found to hardly grow in the temperature range of $900^{\circ}C$ to $950^{\circ}C$, whereas they grow rapidly in the temperature range of $975^{\circ}C$ to $1100^{\circ}C$. (2) Austenite grain growth is considerably small with increasing holding time at a given austenitizing temperature and is, in particular, hardly found to occur at a temperature of $900^{\circ}C$. (3) The hardenability improves ramarkably as Mn content is increased at three different austenitizing temperatures $900^{\circ}C$, $1000^{\circ}C$ and $1100^{\circ}C$. (4) The maximum hardenability is obtained from steels A, B and C austenitized at the $900^{\circ}C$, although Mn content is varied in each specimen.

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