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(-)-Epigallocatechin Gallate Inhibits the Pacemaker Activity of Interstitial Cells of Cajal of Mouse Small Intestine  

Kim, Kweon-Young (Department of Rehabilitation, College of Medicine, Chosun University)
Choi, Soo-Jin (Department of Radiology, Gacheon University Gil Medical Center)
Jang, Hyuk-Jin (Department of Psychiatry, College of Medicine, Chosun University)
Zuo, Dong-Chuan (Department of Physiology, College of Medicine, Chosun University)
Shahi, Pawan Kumar (Department of Physiology, College of Medicine, Chosun University)
Parajuli, Shankar Prasad (Department of Physiology, College of Medicine, Chosun University)
Yeum, Cheol-Ho (Department of Physiology, College of Medicine, Chosun University)
Yoon, Pyung-Jin (Department of Physiology, College of Medicine, Chosun University)
Choi, Seok (Department of Physiology, College of Medicine, Chosun University)
Jun, Jae-Yeoul (Department of Physiology, College of Medicine, Chosun University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.12, no.3, 2008 , pp. 111-115 More about this Journal
Abstract
The effects of (-)-epigallocatechin gallate (EGCG) on pacemaker activities of cultured interstitial cells of Cajal (ICC) from murine small intestine were investigated using whole-cell patch-clamp technique at $30^{\circ}C$ and $Ca^{2+}$ image analysis. ICC generated spontaneous pacemaker currents at a holding potential of -70 mV. The treatment of ICC with EGCG resulted in a dose-dependent decrease in the frequency and amplitude of pacemaker currents. SQ-22536, an adenylate cyclase inhibitor, and ODQ, a guanylate cyclase inhibitor, did not inhibit the effects of EGCG. EGCG-induced effects on pacemaker currents were not inhibited by glibenclamide, an ATP-sensitive $K^+$ channel blocker and TEA, a $Ca^{2+}$-activated $K^+$ channel blocker. Also, we found that EGCG inhibited the spontaneous $[Ca^{2+}]_i$ oscillations in cultured ICC. In conclusion, EGCG inhibited the pacemaker activity of ICC and reduced $[Ca^{2+}]_i$ oscillations by cAMP-, cGMP-, ATP-sensitive $K^+$ channel-independent manner.
Keywords
(-)-epigallocatechin gallate (EGCG); Interstitial cells of Cajal (ICC); Pacemaker currents; Intestinal motility;
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