• Applied Biological Chemistry
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• 제34권3호
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• pp.265-272
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• 1991

어류 가공시 부산물로 얻어지는 어피를 보다 효율적으로 이용할 목적으로 말쥐치피로부터 콜라겐을 추출한 후, 이에 소수성 잔기를 증가시키기 위하여 leucine alkyl ester를 도입하여 기능성 개량을 시도하였다. 말쥐치피 콜라겐 및 FSC-Leu-OCn의 콜라겐 함량, 아미노산 조성에 의한 소수성, 분자량, 용해도, 보수력, 분산성, 지방흡수력, 포말성, 유화성 등을 계면활성제인 Tween-60과 비교 검토한 결과, 어피 콜라겐에 leucine alkyl ester를 도입시킨 $FSC-Leu-OC_8$은 기능성 중 유화성 및 유화안정성이 매우 향상되어 유화제로 이용할 수 있을 것으로 본다.

• Archives of Pharmacal Research
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• 제25권4호
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• pp.469-474
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• 2002

The inner shell of the chestnut (Castanea crenata S. et Z., Fagaceae) has been used as an anti-wrinkle/skin firming agent in East Asia, and preliminary experiments have found that a 70% ethanol extract from this plant material can prevent cell detachment of skin fibroblasts from culture plates. In order to examine the molecular mechanisms underlying this phenomenon, its effects on the expression of adhesion molecules, such as fibronectin and vitronectin, were investigated using the mouse skin fibroblast cell line, NIH/3T3. Using fixed-cell ELISA, Western blotting and immunofluorescence cell staining, it was clearly demonstrated that the chestnut inner shell extract enhanced the expression of the cell-associated fibronectin and vitronectin. Scoparone (6,7-dimethoxycoumarin), isolated from the extract, also possessed similar properties. These findings suggest that the enhanced expression of the adhesion molecules may be one of the molecular mechanisms for how the chestnut inner shell extract preventing cell detachment and may be also responsible for its anti-wrinkle/skin firming effect.

• Journal of Microbiology and Biotechnology
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• 제31권4호
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• pp.630-636
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• 2021

Glabridin, a compound of the flavonoid, has shown outstanding skin-whitening and anti-aging properties, but its water insolubility limits its wide application. Therefore, glabridin liposome (GL) has been developed to improve its poor bioavailability, while there are few studies to evaluate its amelioration of UVB- induced photoaging. This study is performed to investigate the amelioration of GL against UVB- induced cutaneous photoaging. The prepared GL has a spheroidal morphology with an average diameter of 200 nm. The GL shows lower cytotoxicity than glabridin, but it has a more effective role in inhibition of melanin. Moreover, the application of GL can effectively relieve UV radiation induced erythema and leathery skin, associated with the down-regulated expression of inflammatory cytokines (TNF-α, IL-6 and IL-10). Taken together, these results demonstrate that GL has potentials as topical therapeutic agents against UVB radiation induced skin damage through inhibiting inflammation.

• 대한한의학방제학회지
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• 제31권3호
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• pp.183-191
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• 2023

Objective : UVB damages skin health by causing skin redness and intense inflammation, sunburn, and skin cancer. Paeoniae Radix Alba has been used to relieve gynecological symptoms, muscle spasms, and skin ailments. This study was conducted to confirm whether it has a protective effect against UVB photodamage. Methods : Ethanol extract of Paeoniae Radix Alba (PRA) was prepared by extracting 100 g Paeoniae Radix Alba in 1 L of ethanol for 48 h. Apoptosis was monitored by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and expression levels of apoptosis indicator proteins, and tyrosinase activity was measured with a colorimetric commercial kit. Results : In human keratinocyte HaCaT cells, PRA reduced UVB-induced cell death through apoptosis by inhibiting PARP cleavage and caspase-3 and -9. UVB-induced increase in cellular reactive oxygen species (ROS) was suppressed by PRA pretreatment. PRA also showed dose-dependent ABTS and DPPH radical scavenging activities. Furthermore, the inhibitory effect of tyrosinase activity by PRA was confirmed. Conclusion : These results demonstrated the protective role of PRA in UVB photodamage of human keratinocytes, mainly due to its antioxidant and antiapoptotic properties. We also suggest that PRA can be considered as an effective natural agent to prevent skin photodamage.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2006년도 심포지엄 논문집 정보 및 제어부문
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• pp.39-41
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• 2006

It is important to estimate the hand skin temperature because it reveals not only physiological properties of a certain diseases but also it can estimate even human mental-stress conditions. In this study, we try to estimate the temporal skin temperature distribution of human hand by applying stress-cold test to possibly apply to estimate a subject's blood circulation condition in his or her hand in terms of normal or abnormal state.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1994년도 하계학술대회 논문집 C
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• pp.1354-1356
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• 1994

In order to protect cable from rat's biting, a development of rodent repellent is described. This cable has double coated sheath such as construction of skin layer over the conventional cable sheath. Stabilized rodent repellent agent which has good compatibility with PVC is employed in a skin layer. Rodent repellent layer has highly residual properties of agent through the manufacturing process, and meets the general requirements of cable sheath. Also, rat repellent effect is excellent.

• 약학회지
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• 제40권2호
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• pp.155-162
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• 1996

Hyperkeratinization is a dermatologic disorder, which is due to the increase of corneocyte cohesion force. Glycolic acid, an alpha hydroxy acid(AHA), has been used to breakdown the hyperkeratinization processes. However, it has a problem of skin irritation when applied topically, due to the strong acidity especially in high concentration. A molecular optimization of glycolic acid has been tried to reduce the skin irritation by the way of prodrug formation. Ethyl glycolate was synthesized by the esterification of glycolic acid with ethanol in acidic conditions in the presence of sulfuric acid, and examined under the spectroscopic trials, such as UV, IR, $^1H$-NMR, and GC-MS. The physicochemical and biopharmaceutical properties of the prodrug were also evaluated. Through the toxicological tests of both skin irritation and eye mucous irritation, it has been proved that ethyl glycolate was less irritant than glycolic acid, since the pH value of synthetic prodrug was higher than that of glycolic acid. In the penetration test through nude mouse skin by diffusion cell, ethyl glycolate was continuously hydrolyzed to glycolic acid, which was assayed form the receptor compartment. It was obtained that the penetrated amount of ethyl glycolate was five times higher than that of glycolic acid. These results suggest that ethyl glycolate might be a successful prodrug of glycolic acid to reduce the skin irritation and to increase the skin penetration as well.

• KSBB Journal
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• 제28권5호
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• pp.319-326
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• 2013

This study was carried out to investigate the in vitro and in vivo moisturizing properties and percutaneous absorption of PEG-impregnated Aloe vera gel. The PEG-i-Aloe gel was obtained from dewatering and impregnation by soaking (DIS) of Aloe vera leaf slice. The moisturizing property of the obtained sample was evaluated by moisture determination using gravimetric method in desiccator under different RH% and by water sorption-desorption test on human skin. The transdermal penetration characteristics of PEG-i-Aloe gel was investigated by Franz diffusion cell in vitro transdermal absorption method. PEG-i-Aloe gel had high moisture retention ability and could significantly lead the enhancing skin hydration status as well as reducing the skin water loss due to the film formation as a skin barrier. The skin penetration rate of PEGi- Aloe gel at steady state was 9.76 ${\mu}g/(h{\cdot}cm^2)$ and the quantity of the transdermal absorption was 144 ${\mu}g/cm^2$ in 9 hr. The penetration mechanism was well fitted with Higuchi model ($R^2$ = 0.974-0.994). The results show that PEG-i-Aloe gel has the significant moisturizing effect and strong penetration of the animal skin. It could be used as the moisturizing additive in cosmetic skin products.

• 한국식품영양과학회지
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• 제45권8호
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• pp.1147-1152
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• 2016

본 연구는 돈피 젤라틴 분말의 첨가 수준이 떡갈비의 품질특성에 미치는 영향을 규명하고자 실시하였다. 떡갈비의 단백질 함량은 대조구가 가장 낮은 수치를 나타내었으며 돈피젤라틴 분말 4% 처리구가 가장 높은 수치를 나타내었다(P<0.05). 수분 함량은 돈피 젤라틴 분말을 첨가할수록 점차 높아지는 경향을 보였으나, 지방 함량은 돈피 젤라틴 분말 첨가량이 증가함에 따라 점차 감소하는 경향을 나타내었다. 가열 전과 후 명도와 적색도는 돈피 젤라틴 분말 첨가량이 증가함에 따라 유의적으로 감소하였으며, 황색도는 증가하였다(P<0.05). 가열감량, 직경감소율과 두께감소율은 돈피 젤라틴 분말 첨가량이 증가할수록 감소하였으며, 경도는 돈피 젤라틴 분말을 첨가할수록 높은 수치를 나타내었고 3% 처리구와 4% 처리구가 가장 높은 수치를 나타내었다. 검성과 씹음성은 돈피 젤라틴 분말의 첨가량이 증가함에 따라 높은 수치를 나타내었다. 관능적 특성은 모든 돈피 젤라틴 분말을 첨가할수록 색도와 다즙성에서 대조구보다 우수한 평가를 받았으며, 이외 모든 관능평가 항목에서 3% 처리구가 유의적으로 가장 우수한 평가를 받는 것으로 나타났다(P<0.05). 이러한 연구 결과를 바탕으로 돈육 떡갈비에 돈피 젤라틴 분말을 첨가하여 품질 특성을 분석한 결과 2~3%의 돈피 젤라틴 분말을 첨가하여 우수한 떡갈비를 제조할 수 있을 것으로 판단된다.

• 감성과학
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• 제8권4호
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• pp.385-391
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• 2005

화장품을 바를 때 피부와 손가락 사이에서 일어나는 마찰계수는 화장품의 주관적 평가에 많은 영향을 주는 요소이다. in-vivo상태에서 피부와 접촉자 간의 마찰계수는 접촉자의 모양이나 거칠기의 정도 그리고 누르는 하중에 따라 변할 수 있다. 본 실험에서는 리니어 모터와 다축 로드셀을 이용하여 여러 가지 접촉자를 in-vivo상태에서 직접 피부에 접촉시키고 실시간으로 마찰계수를 측정할 수 있는 장치를 개발하였다. 이 장치를 이용하여 피부와 접촉자 간의 마찰계수를 측정하고 그 데이터를 화장품을 바를 때의 전후와 비교$\cdot$분석하였다. 또한 화장품을 바를 때 주관적인 평가에서 이용되는 형용사를 조사하며 마찰계수와의 그 상관관계를 분석하였다. 그 결과, 피부와 접촉자 간의 마찰계수는 화장품의 종류와 접촉자의 성질에 따라 0.17-1.2사이에서 나타나며, 주관적인 평가 또한 화장품을 바른 후 시간의 경과함에 따라 변하며, 그것은 마찰계수와 관련이 있음을 확인할 수 있었다.