• 동의생리병리학회지
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• 제24권2호
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• pp.258-265
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• 2010

Atopic dermatitis (AD) is a chronically relapsing pruritic inflammatory skin disease. To find new anti-inflammatory products for skin inflammatory disease such as AD and contact dermatitis, we produced the effective microorganism fermentation substance (EM-S) by fermentation of medicinal plants with effective microorganisms including photosynthetic bacteria, lactic acid bacteria and yeast, screened the effects of EM-S on NC/Nga model mice. Murine AD-like skin lesions were made by painting Dermatophagoides farinae (Df) extract. Topically applied EM-S significantly reduced clinical severity score, ear thickness and histological grade in AD-like NC/Nga mouse model by Df antigen sensitization. In addition, the serum IgE and Th2 chemokine levels (TARC/CCL17, MDC/CCL22 and CTACK/CCL27) were significantly reduced by EM-S. Futhermore, skin tissue expressions of Th2 chemokines were significantly reduced by EM-S. These results demonstrate that topical application of EM-S may be improve the AD-like skin lesion by suppressing IgE and Th2 chemokines.

• Natural Product Sciences
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• 제12권4호
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• pp.226-231
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• 2006

Artemisia vulgaris, one of the most religious plants in Nepal, is used in the treatment of various ailments. In this study, antioxidative activity of essential oil from A. vulgaris was evaluated, using different in vitro methods and antimicrobial activity by disc diffusion method against skin disease microorganisms such as Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Candida albicans and Propionibacterium acnes. The essential oil exhibited a concentration-dependent antioxidant activity. It showed strong metal chelating activity with low reducing and antioxidant power. However, gradual increase in radical scavenging activity was obtained with increasing concentration and reaction time. It also possessed a broad spectrum of antimicrobial activity and notable susceptibility was observed against S. pyogenes and P. acnes.

• Food Science and Biotechnology
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• 제17권6호
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• pp.1357-1360
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• 2008

Propionibacterium acnes is the predominant organism in sebaceous regions of the skin and is thought to play an important role in the pathogenesis of inflamed lesions. Antibacterial compounds against P. acnes were isolated from the ethanol extract of Kaempferia pandurata Roxb. and identified as panduratin A and isopanduratin A. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of panduratin A for P. acnes were 2 and $4{\mu}g/mL$, respectively, while those of isopanduratin A were 4 and $8{\mu}g/mL$, respectively. The time-dependent killing effect showed that panduratin A and isopanduratin A completely inhibited the growth of P. acnes at 4 and $8{\mu}g/mL$ in 48 hr, respectively. Panduratin A and isopanduratin A demonstrated high antibacterial activities not only against P. acnes but also other skin microorganisms. The results suggest that panduratin A and isopanduratin A could be employed as natural antibacterial agents to inhibit the growth of acne and skin disease causing microorganisms.

• 한국식품위생안전성학회지
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• 제21권1호
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• pp.36-40
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• 2006

온천수에는 각종 무기물이 풍부하게 함유되어 있으며 특히 2가 이온들이 많이 들어있고 살균력을 갖춘 유황 성분이 있어, 식중독 및 병원성 미생물의 증식을 억제할 수 있는 가능성이 있다. 온천수의 식중독 및 병원성 미생물에 대한 억제 효과를 시험하기 위하여 식중독 미생물인 Bacillus cereus, Staphylococcus aureus 및 Escherichia coli, 피부병 원인균인 Candida albicans와 Trichophyton mentogrophytes, 위장병 원인균인 Helicobacter pylori을 대상으로 시험하였다. 본 실험에 사용한 온천수는 먹는물 수질기준에 따라 분석한 결과 불소함량이 기준치 1.5mg/L보다 높은 14.1 mg/L로 먹는물 수질기준에 적합하지 않으나 그 외 항목은 먹는물 수질기준에 적합하였다. 몇 가지 식중독 미생물이 온천수에서 생존하는 양상을 관찰한 결과 S. aureus 및 E. coli의 생존에 대해서는 별 영향을 미치지 않았으나, B. cereus의 균수는 감소시키는데 이는 온천수의 온도($41^{\circ}C$와 관계있는 것으로 판단되었다. C. albicans와 T. mentagrophytes등 피부병균과 위염 원인균(H. pylori)에 대해서도 온천수는 뚜렷한 증식 억제효과를 보이지 않으나 T. mentagrophytes는 온도에 영향이 있음을 확인하였다. 이상의 결과를 종합할 때 온천수는 식중독균이나 피부질환 혹은 위염 원인균에 뚜렷한 억제 효과는 나타나지 않으나 일부 고온($41^{\circ}C$)에 의한 균수의 감소 효과는 인정 되었다.

• 대한본초학회지
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• 제25권2호
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• pp.81-88
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• 2010

Objectives : Sophorae Radix (SR), the dried root of Sophorae Flavescens Aiton, has been used to treat atherosclerosis, arrhythma and skin diseases including scabies and eczema. The present study was examined to evaluate antimicrobial activities of SR extracts against oral microorganism. Methods : Antimicrobial properties of SR extracts were determined by agar diffusion method and minimum inhibitory concentration (MIC) against Streptococcus mutans, Streptococcus sobrinus and Actinomyces viscosus. Analysis of kurarinone from SR extracts was conducted using UPLC (Ultra Performance Liquid Chromatography). Results : The ethanolic extracts of SR showed stronger antimicrobial effect than methanolic extracts, while the aqueous extracts of SR had no activity. In addition, the higher content of kurarinone was found in ethanolic extracts than methanolic extracts. The purified kurarinone from ethanolic extracts showed potent antimicrobial activity with the MIC value of $3.9{\sim}7.8{\mu}g/m{\ell}$. Conclusion : An ethanolic extract of SR showed antimicrobial properties against several oral microorganisms, and kuranrinone contributed to antimicrobial action of SR. Thus, ethanolic extracts of SR or purified kurarinone should be beneficial for the preparation of the useful agent for treating oral disease including anticaries.

• 한국응용과학기술학회지
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• 제37권6호
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• pp.1698-1705
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• 2020

나이가 20-25세인 남녀 성인들 20명(남성 5명, 여성 15명)의 엄지손가락 표면에 상재하는 세균을 채취하고 Luria-Bertani agar에서 배양하였다. 배양된 세균들의 16S rDNA를 polymerase chain reaction을 통하여 증폭하고, 그 염기서열을 분석하였다. 이렇게 얻어진 염기서열을 genbank의 자료들과 비교하여 세균들을 동정한 결과, 총 14종의 세균들이 확인되었다. 개인별로는 평균 2.5종의 배양 가능한 세균들을 손의 피부에 보유하는 것으로 확인되었다. 확인된 세균 중에서는 Staphylococcus 종들이 가장 널리 존재하고 있었으며, 다음으로는 Micrococcus luteus이었다. Staphylococcus 종들의 경우에는 그 분포에 있어서 성별에 따른 차이가 적었으나, Micrococcus luteus의 경우에는 남자보다 여자에게서 훨씬 더 많이 분리되는 경향을 보였다. 이러한 결과는 피부 질환에 대한 연구, 피부질환에 대한 치료제 개발, 피부에 사용하는 화장품개발 등의 분야에 도움이 될 것으로 사료된다.

• Journal of Genetic Medicine
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• 제15권2호
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• pp.92-96
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• 2018

Chronic mucocutaneous candidiasis (CMC) is characterized by increased susceptibility to chronic and recurrent infections of the skin, mucous membranes, and nails by Candida species. It is a primary immunodeficiency disorder that is difficult to diagnose because of its heterogeneous clinical manifestations and genetic background. A 20-month-old boy who did not grow in height for 3 months was diagnosed as having hypothyroidism and he had hepatitis which was found at 5 years old. He presented with persistent oral thrush and vesicles on the body, the cause of which could not be identified from laboratory findings. No microorganism was detected in the throat culture; however, the oral thrush persisted. Immunological tests showed that immunoglobulin (Ig) subclass IgG and cluster of differentiation (CD)3, CD4, and CD8 levels were within normal limits. We prescribed oral levothyroxine and fluconazole mouth rinse. The patient was examined using diagnostic exome sequencing at the age of 6 years, and a c.1162A>G (p.K388E) STAT1 gene mutation was identified. A diagnosis of CMC based on the STAT1 gene mutation was, thus, made. At the age of 8 years, the boy developed a malar-like rash on his face. We conducted tests for detection of antinuclear antibodies and anti-dsDNA antibodies, which showed positive results; therefore, systemic lupus erythematosus (SLE) was also suspected. Whole exome sequencing is important to diagnose rare diseases in children. A STAT1 gene mutation should be suspected in patients with chronic fungal infections with a thyroid disease and/or SLE.

• 한국환경보건학회지
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• 제50권2호
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• pp.102-112
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• 2024

Background: Despite the rise in the number of domestic indoor climbing gyms, there is a lack of specific hygiene standards and research on the holds installed in them. Holds can act as vectors for microbial transmission through the hands, posing a risk of infectious diseases, especially with damaged skin. Objectives: The aim of this study is to investigate the contamination level and species of microorganisms on holds according to the management methods practiced in indoor climbing gyms and identify effective strategies for reducing microbial contamination. Methods: We investigated factors that may influence microbial contamination of holds, including hold management methods, user information, and hygiene management at three climbing gyms in Seoul. A total of 72 holds were sampled, 18 for each management method of brushing, high-pressure washing, and ethanol disinfection. Samples were cultured on LB and blood agar at 37℃ for 48 hours to calculate CFUs. PCR assay targeting 16S rRNA was carried out to identify microorganisms. Dunn-Bonferroni was employed to see the microbial reduction effect of the management method and the difference in microbial contamination by management method and climbing gym. Results: As a result of microbial identification, microorganisms such as Bacillus, Staphylococcus, and Micrococcus, which were derived from various environments such as skin and soil, were discovered on the surface of the climbing hold. Among the discovered microorganisms, some species had potential pathogenic properties that could cause food poisoning, gastrointestinal disease, bacteremia, and sepsis. All hold management methods were effective in reducing microorganisms (p<0.05), with ethanol disinfection being the most effective (p<0.001). Conclusions: Our results indicate that there are potential pathogens on holds that demand thorough management for microbial prevention. Proposed methods include regular brushing and ethanol disinfection in addition to high-pressure washing with long cycles, which are the existing forms of hold management. Further studies on shoe management are advised to curb soil-derived microorganisms.