• 제목/요약/키워드: skin condition

검색결과 949건 처리시간 0.032초

제주흑우 체세포 복제수정란의 체외 생산 (In Vitro Production of Jeju Black Cattle Cloned Embryos by Somatic Cell Nuclear Transfer (SCNT))

  • 김동훈;양병철;임기순;류재규;노진구;박종주;이성수;고문석;박진기
    • 한국수정란이식학회지
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    • 제27권3호
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    • pp.149-154
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    • 2012
  • This study was carried out to investigate effective condition for producing somatic cell nuclear transfer (SCNT) embryos of Jeju native cattle. As donor cells for SCNT, ear skin cells from Jeju native cattle were used. In experiment 1, the effect of recipient oocyte sources on the development of Jeju native cattle SCNT embryos were examined. Fusion rate of recipient oocyte and donor cell was not different between the Hanwoo and Holstein recipient oocytes (86.0% vs 89.9%). The rate of embryos developing to the blastocyst stage was significantly (p<0.05) higher in Hanwoo recipient oocytes than in Holstein recipient ones (28.2% vs 14.7%). Blastocysts derived from Hanwoo recipient oocytes contained higher numbers of total cells than those derived from Holstein ones ($115.1{\pm}40.8$ vs $101.4{\pm}33.3$), although there were no significant difference. The mean proportion of apoptotic cells in blastocyst was not different between the sources of recipient oocytes. In experiment 2, the development of Jeju native cattle and Hanwoo SCNT embryos were compared. Hanwoo oocytes were used as the recipient oocytes. Fusion rate was not different between the Jeju native cattle and Hanwoo SCNT embryos (92.1% vs 92.9%). The blastocyst rate of SCNT embryos was significantly (p<0.05) lower in Jeju native cattle than in Hanwoo (16.9% vs 31.0%). Blastocysts derived from Jeju native cattle SCNT embryos contained smaller numbers of total cells than those derived from Hanwoo ones ($136.6{\pm}33.7$ vs $149.9{\pm}39.7$), but there were no significant difference. The mean proportion of apoptotic cells in blastocyst was not different between the Jeju native cattle and Hanwoo SCNT embryos. The present study demonstrated that Hanwoo recipient oocytes were more effective in supporting production of Jeju native cattle SCNT embryos, although Jeju native cattle SCNT embryos showed reduced developmental capacity when compared to Hanwoo SCNT embryos.

선박의 비선형 유체력을 고려한 파랑중 동적 강도 해석법에 관한 연구 (A Study on the Dynamic Strength Analysis of the Hull Girder Among Waves Considering Non-Linear Hydrodynamic forces)

  • 신구균;김사수;손성완
    • 대한조선학회논문집
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    • 제29권4호
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    • pp.152-172
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    • 1992
  • 대파고 파랑중을 항해하는 선박은 큰 선체 운동으로 인하여 수면하 단면 형상이 시시각각 크게 변하므로 자유 표면 조건, 물체 표면 조건의 비선형성에 의한 비선형 유체력의 영향이 무시될 수 없게 된다. 경우에 따라서는 선저가 파면으로부터 충격력을 받는 슬래밍 현상과 선수가 파도를 뒤집어 쓰는 청파 현상등과 같은 충격적 유체력이 선체에 가해지는 등 복잡한 문제가 발생하게 된다. 본 연구에서는 선체를 가변 단면보의 탄성체로 이상화하여 파랑중 선체 거동을 박육 단면보 이론에 의해 정식화하고 파랑 하중으로는 수면하 단면 형상 변화에 따른 비선형 유체력과 momentum slamming이론을 이용한 유체 충격력을 고려하여 대파고 파랑 중 탄성체인 선체의 응답을 추정하는 해석 기법을 개발하여 이를 기존의 실험결과와 비교 그 타당성을 확인하고, 이의 응용으로 본 기법에 의하여 4만톤급 정유 운반선에 적용하여 정면파 및 사파중에서 파고, 파장, 선속을 파라미터로 한 수치 계산을 수행하고 여러가지 파라미터 변화에 대한 선체 구조의 동적 강도 응답 특성을 계통적으로 분석하여 보았다. 본 연구에서 개발된 동적강도 해석법은 대파고 중에서 유체력의 비선형성 및 유체 충격력까지 고려한 해석기법이므로 신구조 방식 선박에 대한 직접 설계법의 확립 뿐만 아니라 슬래밍 등에 의한 선체 절손 사고의 원인 규명에도 유용하게 적용할 수 있을 것으로 사료된다.

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한 광역시 종합병원 근로자의 VDT 증후군 자각증상에 대한 관련요인 연구 (Related Factors to Visual Display Terminal Syndrome in Employees of A General Hospital in one Metropolitan City)

  • 유세종;홍지영;배석환
    • 대한방사선기술학회지:방사선기술과학
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    • 제34권4호
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    • pp.297-303
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    • 2011
  • 병원 근로자의 VDT 자각 증상에 대한 선행연구를 보면 대부분이 간호사 관련 직종으로 한정되어 있고, 방사선사에 대한 연구는 수행되지 않았다. 본 연구는 2009년 10월 15일부터 11월 2일까지 대전광역시에 소재하고 있는 종합병원에 VDT 작업을 시행하는 보험심사, 원무, 의무기록, 전산, 행정부서 근무자와 방사선사를 대상으로 구조화된 설문지를 활용하여 분석하였다. 연령이 20~29세, 임상경력이 5년 이하, 일반직원이며, 미혼인 대상자가 VDT 증후군 자각증상 중 피부, 심리 증상에서 유의한 차이를 보였으며, 연령이 30~39세, 임상경력이 6~10년, 관리자, 기혼인 대상자가 VDT 증후군 자각증상 중 안관련 증상, 전신 증상, 근골격 증상에서 유의한 차이를 보였다. VDT 증후군 자각 증상 점수를 종속변수로 한 다중선형회귀 분석 결과 관련 요인으로 안관련 증상에 영향을 미치는 요인을 보면 성별, 건강염려, 일상생활 방해이며, 전신증상은 운동, 스트레스, 건강상태 인지 관련, 근골격 증상에서는 임상경력 6~10년, 대인관계, 건강상태 인지 관련 건강염려와 일상생활 방해로 나타났으며, 통계적으로 유의한 차이를 보였다. 타부서 근로자와 방사선사의 VDT 증후군 자각 점수 차이가 크게 나타나지 않았다는 것은 방사선사의 업무가 VDT 작업환경으로 변함으로써 VDT 증후군 자각 증상이 일어날 수 있다고 말할 수 있다. 이는 정기적인 순환근무를 시행함으로써 VDT 작업환경에 지속적인 노출을 피하는 것이 예방 대책이라 할 수 있다.

금궤요략${\cdot}$수기병맥증병치제십사(水氣病脈證幷治第十四)에 대한 연구 (Study On the 'Diagnosis and Trearment of Edema' in the Chapter 14 of Synopsis of Golden Chamber)

  • 노승조;박금숙;권미자;이영섭;정헌영
    • 동의생리병리학회지
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    • 제22권1호
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    • pp.60-81
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    • 2008
  • This thesis is written about the causes, the processes and the treatments of edema. It consists of 31 chapters. Covering 11 chapters, there are prescriptions and constructions. When one of the organs fails to work metabolizing water, you can have edema. It is the result of the accumulation of excess fluid under the skin. Edema most commonly occurs in the head facial, the palpebral portion, the feet and legs or all the body. Sometimes it is accompanied with ascites. There are three classifications in the thesis. First, according to the cause and the symptom of disease, it is divided into 5 types. Type 1. The swelling caused by the disorder of the lungs from the troubling wind. Type 2. The swelling caused by the weak spleen. Type 3. The swelling caused by abnormal condition between the kidneys and the lungs. Type 4. The swelling in abdomen caused by the weak kidneys. Type 5. The swelling (Yellow sweat) caused by the heat inside body as water outside. Second, the swelling and the abnormal symptom of five viscera. Third, the swelling accompanied with menstruation in female and with chilliness. In short, the original textbook is showed that the treatment of edema focuses on the cause and the region of fluid accumulation. When the swelling is in the upper body or the cause of diseaseis outside, the useless humidity can be expelled through sweating. On the other hand, when the swelling is in the lower body or the cause of disease is inside, it is pushed out by urination. In this book, the author presents further treatments. And these treatments are often cited by doctors after ages. Even one of them suggested that the swelling be divided by cosmic dual forces. In spite of many researches, it is too complicate for us to understand the writer’s intend. For that reason, comparing with other medical books and referring to guides explained by doctors, I have studied the textbook to help understand.

1-Methylcyclopropene 처리와 미세천공필름 포장이 큰느타리버섯의 저장기간 중 품질에 미치는 영향 (Effect of 1-methylcyclopropene and microperforated film packaging on postharvest quality of king oyster mushroom (Pleurotus eryngii))

  • 최지원;전창성;홍윤표;조미애;김지강
    • 한국버섯학회지
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    • 제10권4호
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    • pp.191-197
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    • 2012
  • 수확후 큰느타리버섯의 3주 이상 저온 저장 및 유통 중 품질 유지를 확보하기 위하여 포장 내부의 적정 산소와 이산화탄소 농도를 유지하는 MAP 기술적용 체계를 확립하고자 하였다. 이를 위하여 $1{\mu}L/L$ 1-methylcyclopropene (1-MCP, 20시간, $0^{\circ}C$)을 처리하고 방담성 $30{\mu}m$ oriented polypropylene(OPP, $1238.0cc/m^2{\cdot}day{\cdot}atm\;O_2$)필름과 미세천공(MP3, $3179.9cc/m^2{\cdot}day{\cdot}atm\;O_2$)필름으로 포장한 후 $0^{\circ}C$에 저장 3주, 4주 저장 후 3일 상온유통을 실시하고 큰느타리버섯의 색도, 경도, 이취 등 품질변화를 관찰하였다. 1-MCP 처리 후 미세천공필름 포장에 의해서 저장 기간 동안 포장재 내 산소 농도 3.6~10.9%, 이산화탄소 농도 9.0~13.8% 수준이 유지되었으며 4주 저장 + 3일 상온유통 후 무처리 OPP필름보다 갓무름과 갈변, 이취 발생이 적어 상품성을 유지하였다.

Research on the Multi-electrode Plasma Discharge for the Large Area PECVD Processing

  • Lee, Yun-Seong;You, Dae-Ho;Seol, You-Bin
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2012년도 제42회 동계 정기 학술대회 초록집
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    • pp.478-478
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    • 2012
  • Recently, there are many researches in order to increase the deposition rate (D/R) and improve film uniformity and quality in the deposition of microcrystalline silicon thin film. These two factors are the most important issues in the fabrication of the thin film solar cell, and for the purpose of that, several process conditions, including the large area electrode (more than 1.1 X 1.3 (m2)), higher pressure (1 ~ 10 (Torr)), and very high frequency regime (VHF, 40 ~ 100 (MHz)), have been needed. But, in the case of large-area capacitively coupled discharges (CCP) driven at frequencies higher than the usual RF (13.56 (MHz)) frequency, the standing wave and skin effects should be the critical problems for obtaining the good plasma uniformity, and the ion damage on the thin film layer due to the high voltage between the substrate and the bulk plasma might cause the defects which degrade the film quality. In this study, we will propose the new concept of the large-area multi-electrode (a new multi-electrode concept for the large-area plasma source), which consists of a series of electrodes and grounds arranged by turns. The experimental results with this new electrode showed the processing performances of high D/R (1 ~ 2 (nm/sec)), controllable crystallinity (~70% and controllable), and good uniformity (less than 10%) at the conditions of the relatively high frequency of 40 MHz in the large-area electrode of 280 X 540 mm2. And, we also observed the SEM images of the deposited thin film at the conditions of peeling, normal microcrystalline, and powder formation, and discussed the mechanisms of the crystal formation and voids generation in the film in order to try the enhancement of the film quality compared to the cases of normal VHF capacitive discharges. Also, we will discuss the relation between the processing parameters (including gap length between electrode and substrate, operating pressure) and the processing results (D/R and crystallinity) with the process condition map for ${\mu}c$-Si:H formation at a fixed input power and gas flow rate. Finally, we will discuss the potential of the multi-electrode of the 3.5G-class large-area plasma processing (650 X 550 (mm2) to the possibility of the expansion of the new electrode concept to 8G class large-area plasma processing and the additional issues in order to improve the process efficiency.

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Over-expression of MMP-3 in the fissured tissue of cleft lip and palate

  • Park, Young-Wook;Min, Bong-Gi;Kim, Ji-Hyuck;Kim, Soung-Min;Lee, Young-Joon;Lee, Sang-Shin;Lee, Suk-Keun;Moon, Huck-Soo;Chi, Je-Geun
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • 제32권1호
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    • pp.19-26
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    • 2006
  • Objective: In order to elucidate the retrogressive degeneration of orofacial cleft, the fissured tissues of prenatal and postnatal cleft lip and palate were examined by histological and immunohistochemical methods. Design: Totally 42 cases of prenatal (n=17) and postnatal (n=25) cleft lip and/or palate were examined in comparison with 10 cases of normal lip and oral mucosa using immunohistochemical stainings of MMP-3, MMP-9, MMP-10, cathepsin G, PCNA, E-cadherin, TGase 2, HSP-70, vWF, and VEGF. Main Outcome Measures: In the fissured tissue the sebaceous glands were strongly positive for PCNA and grew into the underlying fibromuscular tissue (24/42). Some hyperplastic sebaceous glands of prenatal cleft lip produced infundibular follicular cyst (9/17). The skin and mucosal epithelia from the postnatal cleft lip and palate (10/25) showed severe basal hyperplasia (11/25) and melanocyte infiltration (7/25). Results: The immunostaining of MMP-3 and HSP-70 were strongly positive in the hyperplastic sebaceous glands and nearby atrophying muscle bundles of the fissured tissue, while MMP-9, MMP-10, and cathepsin G were almost negative. The immunoreactions of the other antibodies used in this study were similar between in the fissured tissues and in the normal controls. Conclusions: These data suggest that the over-expression of MMP-3 is closely related to the sebaceous gland hyperplasia, epithelial dysplasia, and the muscle degeneration, and that the over-expression of MMP-3 in the fissured tissue may continuously aggravate the cleft condition in the later life.

육계 도축시 전기실신 방법이 육질에 미치는 영향 (The Effect of Different Electrical Stunning Method§ on Meat Quality in Broilers)

  • 안종남;채현석;유영모;조수현;김영태;이종문;최양일
    • 한국축산식품학회지
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    • 제23권3호
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    • pp.221-226
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    • 2003
  • 본 연구는 도계과정 중 전압의 차이가 육계의 육색에 미치는 영향을 조사하기 위하여 육계 180수를 50과 65V,그리고 90V, 255 Hz, 5 초 조건으로 각각 60수씩 배치하여 실신시킨 후 pH, 보수력, 가열감량, 육색(껍질, 가슴육, 다리육, 날개육)과 혈반(Blood spot) 출현율을 조사하였다. 전기자극에 의한 기절시 전압의 차이에 의한 pH, 보수력과 가열감량에 대한 영향에서 저전압(50V, 255 Hz, 5 초)에 의한 전기자극은 고전압(90V, 255 Hz, 5 초)에 비하여 높은 pH와 보수력 그리고 낮은 가열감량을 보이는 것으로 나타났으나 유의적 차이는 없었다(p>0.05). 50V, 255 Hz, 5초에서 90V, 255 Hz, 5초 동안의 전압의 변화는 부분육별 육색에 있어서는 닭의 껍질, 가슴육과 날개육에는 큰 영향을 미치지는 않았다. 그러나 다리육에 있어서 전압의 증가는 명도와 황색도를 유의적으로 증가시켜 주는 것으로 나타났다(p<0.05). 혈반의 발생율은 미지선(50.56%)과 날개(54.77%)에서 높은 발생율을 보였으며, 다리(8.89%)와 가슴(4.44%)의 표면에서는 낮은 발생율을 보였지만 전압의 변화에 따른 일정한 경향은 보이지 않았다. 본 실험의 결과 도계시 전압의 변화는 닭고기의 pH, 보수력, 육색과 혈반 발생율에 유의적 차이는 없었지만, 다소간에는 영향이 있는 것으로 나타났다. 따라서 전압의 세기, 자극시간, 전류의 세기 등의 복합적 요인을 고려한 다각적 분석이 필요할 것으로 사료되었다.

서울 지역 고등학생의 영양소 섭취와 체위 및 혈액성상 (A Study on Nutrient Intake, Anthropometric Data and Serum Profiles among High School Students Residing in Seoul)

  • 이현숙;김보은;조미숙;김화영
    • 대한지역사회영양학회지
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    • 제9권5호
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    • pp.589-596
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    • 2004
  • This study was performed to investigate the anthropometric data, serum profiles, and nutrient intakes of high school students who are living in Seoul. Anthropometric data showed that mean height and weight were 174.1 cm and 66.6 kg in 99 male and 161.4 cm and 54.0 kg in 116 female students. Mean BMl for male and female students were 21.9 and 22.3 kg/$m^2$, and subjects with BMI > 25 kg/$m^2$ were 14 and 7%, respectively. Total cholesterol, HDL- and LDL-cholesterol of subjects were 149.6$\pm 3.1,\; 46.4 \pm 1.3,\; 86.4 \pm 2.3$,/TEX> mg/dl for males and 169.2 $\pm 3.1,\;50.7 \pm 1.0,\; 100.9 \pm 2.7 mg/dl$ for females, respectively. Hemoglobin (Hb) and total iron binding capacity (TIBC) were 12.8 $\pm 0.1 g/dl, \;437.3 \pm 7.2\mug/dl\; in\; male\; and\; 11.9 \pm 0.1 g/dl,\; 439.4 \pm 5.7\mug/dl$ in female, respectively. Based on Hb classification, 45.6% of male and 45.0% of female students fell into anemic condition. Intakes of energy, Ca, Fe, Zn, riboflavin, and folate were below the Korean RDA. In particular Ca (male and female, respectively, 64.6 and 64.3% RDA) and Fe (male and female, respectively, 78.6 and 64.3% RDA) intakes were extremely low. Serum triglyceride was positively significantly correlated with weight (r =0.22), BMI (r =0.279), waist circumference (WC, r =0.235), triceps skin-fold thickness (TSF, r =0.197) and obesity index (OI, r =0.279). Hb concentration was positively correlated with height (r + 0.387), weight (r =0.349), BMI (r =0.191) and waistlhip ratio (WHR, r =0.380). As for the correlation between serum profiles and nutrient intakes, the Hb concentration was positively correlated with energy (r =0.163), protein (r =0.149), Na (r =0.153), vitamin A (r =0.165), thiamin (r =0.201) and niacin (r =0.192, p < 0.01). These result suggest that the prevalence of Ca and Fe deficiency of student is high and dietary guideline for prevent anemia in this age needs to contain the adequate intake of energy and its related vitamins.

정.기(精.氣) 양생법(養生法)에 관한 문헌연구 (Literature Review on Spirit and Qi Regimen)

  • 백숙희;석소현;오혜경;문희자
    • 동서간호학연구지
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    • 제2권1호
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    • pp.96-106
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    • 1997
  • In Chinese medicine, the activities of removing and solving the inner cause are called the regimen in preventing the disease and the good condition of mind emphasizes us to practice ourselves the doctrine of regimen of the mind to promote the health and long life of the living body, in other words, it means we should have clean mind, less desire, less thought, less agony and widely open our emotion and will, and it also means the raising of good nature, the moral cultivation, and we can be healthy when we live by good virtus as the root of regiem. The meaning of the Qi has been expanded more and more since its origination throught the process of practice and perception of man. 1) Matter is changed to the smoke when burned, the energy of water becomes the rain in the sky and the rain raises all living creatures by making them wet. Throught these changes of circulation, men could realize the energy to be the common and original matter of forming all the creatures. 2) The direction of showing the breathing of men in and out has been expended. 3) It was widely understood as the meaning of showing vitality of men, and it was the original root of chinese medicine. 4) It was expended to be showing spirit of nature such as the sun, moon, star, sky, land, mountain and the moral spirit of men as peacefullness. By the original meaning of the word of energy expanded to the wider side of sky, land, men and things, nature, society, man and the moral spirit could get the unified basis of the matter. As the above, the word of Qi has been used in wider meaning at this time from the past. In other words, all things in the universe come into being and extinct by the chang of motion of the Qi and it is recognized to be the living activity in human body. The Qi-kong based on this energy and the motion applied to our daily life are very extensive (Lee, Hye - lung, 1997). Here are the summaries of the effect of the Qi-kong ; 1) The physical constitution is strenthened, physiological function is prosperous and we are free from various geriatric disease and psychosomatic disease not to mention the cold and indigestion. 2) It maintains the clear spirit, elevates intelligence, strengthens the spiritual power and demonstrates the potentiality at the amximum. 3) It maintain beautiful figure and clean skin never losing the charm but full of energy and vitality. 4) It keeps the balanced body never suffering from the unbalance of the bones, various and neuralgia. 5) It maintain the spiritual comfort, the natural posture in everything and real happiness (Lee, Hye-Jung, 1997 : Suh, Yong Kyu, 1989). Chinese medicine mentions the motion by Qi with Qi - kong. But the motion does not require special method in Western medicine. I t is the only way of living of our ancestor indaily life. It is maintaining the healthy lifr by training the Qi, and it is the motion of being with the nature with the open mind in breathing rather than artificially restricting our daily life, it is the motion of the body.

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