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Over-expression of MMP-3 in the fissured tissue of cleft lip and palate  

Park, Young-Wook (Department of Oral and Maxillofacial Surgery, College of Dentistry, Kangnung National University)
Min, Bong-Gi (Department of Oral and Maxillofacial Surgery, College of Dentistry, Kangnung National University)
Kim, Ji-Hyuck (Department of Oral and Maxillofacial Surgery, College of Dentistry, Kangnung National University)
Kim, Soung-Min (Department of Oral and Maxillofacial Surgery, College of Dentistry, Kangnung National University)
Lee, Young-Joon (Department of Oral Pathology, College of Dentistry, Kangnung National University)
Lee, Sang-Shin (Department of Oral Pathology, College of Dentistry, Kangnung National University)
Lee, Suk-Keun (Department of Oral Pathology, College of Dentistry, Kangnung National University)
Moon, Huck-Soo (Department of Preventive Dentistry, College of Dentistry, Seoul National University)
Chi, Je-Geun (Department of Pathology, College of Medicine, Seoul National University)
Publication Information
Journal of the Korean Association of Oral and Maxillofacial Surgeons / v.32, no.1, 2006 , pp. 19-26 More about this Journal
Abstract
Objective: In order to elucidate the retrogressive degeneration of orofacial cleft, the fissured tissues of prenatal and postnatal cleft lip and palate were examined by histological and immunohistochemical methods. Design: Totally 42 cases of prenatal (n=17) and postnatal (n=25) cleft lip and/or palate were examined in comparison with 10 cases of normal lip and oral mucosa using immunohistochemical stainings of MMP-3, MMP-9, MMP-10, cathepsin G, PCNA, E-cadherin, TGase 2, HSP-70, vWF, and VEGF. Main Outcome Measures: In the fissured tissue the sebaceous glands were strongly positive for PCNA and grew into the underlying fibromuscular tissue (24/42). Some hyperplastic sebaceous glands of prenatal cleft lip produced infundibular follicular cyst (9/17). The skin and mucosal epithelia from the postnatal cleft lip and palate (10/25) showed severe basal hyperplasia (11/25) and melanocyte infiltration (7/25). Results: The immunostaining of MMP-3 and HSP-70 were strongly positive in the hyperplastic sebaceous glands and nearby atrophying muscle bundles of the fissured tissue, while MMP-9, MMP-10, and cathepsin G were almost negative. The immunoreactions of the other antibodies used in this study were similar between in the fissured tissues and in the normal controls. Conclusions: These data suggest that the over-expression of MMP-3 is closely related to the sebaceous gland hyperplasia, epithelial dysplasia, and the muscle degeneration, and that the over-expression of MMP-3 in the fissured tissue may continuously aggravate the cleft condition in the later life.
Keywords
Human; Fetal; Adult; Cleft lip and palate; Sebaceous gland; MMP-3;
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