• Archives of Plastic Surgery
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• v.49 no.3
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• pp.304-309
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• 2022

Background Laminin 5, which is found in the basement membrane of dermal-epidermal junction (DEJ), is a major adhesive component and associated with proliferating and migrating keratinocytes. In this study, we hypothesized that the topical application of the skin care products containing the novel peptides might restore the DEJ structure by deriving deposition of laminin 5 and promoting the keratinocyte migration. Here, we evaluated the restoration of DEJ by measuring the skin thickness. Methods Single-center retrospective analysis was performed on a total of 13 patients who underwent skin care using Baume L.C.E. (France, Laboratories d' Anjou) between January and March 2021. All patients applied the skin care agent for 2 weeks only on their left hand dorsum. Before the initiation of the application and after 2 weeks, both their hands were evaluated on photography and ultrasound. And the patients were asked to rate their satisfaction with the questionnaire after 2 weeks. Results There was no obvious improvement in photographic assessment and questionnaire. The post-pre difference of skin thickness in ultrasound images was, in left hand, 0.1 ± 0.37 in distal point and 0.1 ± 0.35 in proximal point; and, in right hand, 0 ± 0.17 in distal point and 0 ± 0.15 in proximal point, respectively. The pre-post difference was statistically significant in proximal point (p = 0.035). Conclusion Topical application of novel peptide derivative comprising laminin 5 demonstrated cutaneous changes including skin thickness, as assessed by ultrasound. Further studies using other modalities including dermal density measurement, three-dimensional photography, optical coherence tomography, or skin biopsy would be helpful to determine the skin-improving effects.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.27 no.1
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• pp.53-72
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• 2001

Dry skin is caused mainly by the perturbation of stratum corneum lipids which affected by ageing, change of season, excess use of surfactant and the effect of disease like atopic dermatitis and psoriasis. Intercellular lipid structures in stratum corneum are responsible for the barrier function of mammalian skin. The major lipd classes that can be extracted from stratum corneum are ceramides, cholesterol and fatty acid, which make up approximately 50, 25, 10 percent of the stratum corneum lipid mass, respectively. Small amount of cholesterol sulfate, phospholipids, glycosylceramide and cholesterol esters are also present. Recent studies have shown that application of one or two these lipids to the perturbed skin delays barrier recovery; only equimolar mixtures allow normal recovery. We observed that barrier recovery rate was improved in hairless mouse by topical application of single neutral lipids (ceramide, free fatty acid, cholesterol) and lipid mixtures. Whereas the application of single lipid didn’t allows a significant enhancement comparing with normal barrier repair, the equimolar mixtures of 3 components(including synthetic pseudoceramide PC104) improved barrier repair, as assessed by the transepidermal water loss. At clinical study to the volunteers aged over sixty, skin dryness recuperated by the increase of moisture(capacitance) and the reduction of scaling. Utilization of physiologic lipid mixture containing natural ceramides or synthetic pseudoceramide could lead to new forms of topical therapy for the dryness and dermatoses(e.g., psoriasis, atopic dermatitis and irritant dermatitis).

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.6
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• pp.1611-1620
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• 2008

This clinical research was conducted to test patients with Atopic Dermatitis by external application with Hwangryeonhaedok-Tang in cosmetics. We gave scores to 31 patients who visited the Dept. of Oriental Medical Opthalmology & Otolaryngology & Dermatology of Semyung University Jecheon Oriental Medical Hospital from May 15th, 2008 to June 26th. Fifteen patients were treated with the ointment that contain Hwangryeonhaedok-Tang (experimental group) and sixteen patients were treated with normal ointment that doesn't have Hwangryeonhaedok-Tang (control group) for 4 weeks. We observed change of total IgE, eosinophil count, Skin Temperature, Transepidermal Water Loss(TEWL), Skin Hydration and Skin pH. Also Clinical Index of Atopic Dermatitis(SCORAD Index) and global assesment of efficacy were used to evaluate the effects of Hwangryeonhaedok-Tang. Statistical analysis was performed by using frequency analysis and descriptive analysis. Statistical significance was achieved if the probability was less than 5%(p<0.05). After 4 weeks of external application treatment, SCORAD Index in experimental group was significant statistically decreased compared with control group. After 4 weeks of external application treatment, total IgE of both groups were decreased and eosinophil count in control group was decreased but experimental group was unstatistically decreased. Unstatistically, both groups didn't showed significant effect on Skin Temperature. Transepidermal Water Loss(TEWL) in both groups were increased but experimental group was significant decreased compared with control group. Skin Hydration in experimental group was significant statistically increased compared with control group. Statistically, both groups didn't showed significant effect on Skin pH. Only experimental group showed little increase. After 4 weeks of external application treatment, experimental group showed significant effect on global assesment of efficacy. Considering the above results, we can speculate that cosmetics with Hwangryeonhaedok-Tang has some therapeutical effects in mitigating the symptoms of Atopic Dermatitis.

• Archives of Plastic Surgery
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• v.33 no.5
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• pp.601-605
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• 2006

Purpose: Large skin defect by various causes, should be covered by autologous skin graft. But, the donor site of autologous skin graft is limited and leaves permanent donor scar and contracture. There have been our trial to engineer artificial skin using allogenic dermis (AlloDerm) with basement membrane. Methods: Dermal and epidermal layer were separated by immersing in dipase solution for 30 minutes, and the separated layers were treated with 0.05% trypsin for 10 minutes. And then each layer was cultivated to fibroblasts and keratinocytes on a culture medium. Fibroblasts were first penetrated into basement membrane of allogenic dermis facing down, then allogenic dermis was flipped over to face up and keratinocytes were transplanted to allogenic dermis. Results: Observing artificial skin fabricated in vitro, we found following: 1) The artificial skin opened in air for 5 days formed epidermal layer. In dermal layer, fibroblast was distributed evenly among all. 2) The artificial skin opened in air for 30 days formed thicker and thicker, and it formed basement membrane, spinous and granular layers. PAS stain to confirm existence of basement membrane showed positive reaction. 3) Cytokeratin 10 stain to confirm the formation of epidermal layer showed positive reaction. 4) The formation of thick keratin, lamellar body and desmosome similar to human skin were observed in result of an electron micrograph. Conclusion: As a result of research, the structure seen in normal skin such as rete ridge, is found in reproduced artificial skin. This type of artificial skin can be used as a useful model for investigating skin disease and for clinical application also.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.18 no.1
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• pp.28-36
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• 2011

Purpose: The purpose of this study were to develop a new restraint for the intensive care units (ICU) and to investigate the application effect in comparison with a control group using existing restraints. Method: A non-equivalent control group non-synchronized quasi-experimental research design was used. The participants were 40 (control 20, experimental 20) patients who were recruited by convenience sampling the ICU of a university hospital. To avoid contamination of the experiment, data for the control group were collected prior to the experimental group. Measurement variables were edema and skin damage (redness and abrasion) at the application site, and nurses' perceived convenience in applying restraints. Results: Three days after applying the restraint, amount of edema at the application site was small and incidence of skin damage decreased in the experimental group in comparison with the control group. Also, score for application convenience measured by the nurses was higher in for the newly developed restraint than for existing restraints. Conclusion: Results indicate that the newly developed restraint has lower effects such as edema and skin damage and is more convenient compared with existing restraints, and is therefore recommended for patients in the ICU.

• KIEAE Journal
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• v.15 no.3
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• pp.43-48
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• 2015

Purpose: Recently, many countries around the world are actively looking for the ways to make full use of natural energy sources and also develop and apply an environmentally friendly system designed to save building energy consumption. Under these circumstances, this study intended to determine the applicability and energy saving effect by deriving the indoor thermal performance characteristics and the PCM temperature appropriate for a double skin façade to reduce indoor energy consumption through the application of different PCM temperatures to double skin façade and perform a performance evaluation depending on the application or non-application of PCM to a double skin façade. Method: For this study, the physical variables of the double skin façade with PCM were configured through a preliminary examination based on an experimental measurement, and experimental measurements were taken with a total of 7 types of mockup cases: Type-1 (Basic), the basic double skin façade, Type-2 (PCM $18^{\circ}C$) which was applied to the inner skin of the double skin façade depending on the phase-change temperature of PCM, Type-3 (PCM $20^{\circ}C$), Type-4 (PCM $22^{\circ}C$), Type-5 (PCM $24^{\circ}C$), Type-6 (PCM $26^{\circ}C$), and Type-7 (PCM $28^{\circ}C$) with reference to the data analysis of the basic double skin façade which preceded this study, to analyze the indoor thermal performance of the double skin façade depending on PCM temperature and the installation or non-installation of a double skin façade applying PCM based on the selected unit space. Result: Indoor thermal performance was analyzed depending on the PCM temperature applicable to double skin façade, and the analysis of heating energy reduction showed that Type-2 (PCM $18^{\circ}C$) gained 15.9% more heat compared with Type-1 (Basic) and secondly, Type-3 (PCM $20^{\circ}C$) gained 11.5% more heat. Based on these findings, it is deemed possible that the use of energy for heating can be reduced when heat coming indoors increases during the heating period, and the appropriate temperature for PCM applied to the inner skin of a double skin façade to reduce heating energy in winter, Type-2 (PCM $18^{\circ}C$) showed the highest efficiency and Type-3 (PCM $20^{\circ}C$) was also deemed appropriate.

• Journal of Veterinary Clinics
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• v.18 no.4
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• pp.363-367
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• 2001

Shampoos are used routinely by a large number of veterinarians to treat skin diseases. Skin pH is affected by shampoos, however, known to occur. In order to evaluate the effect of shampoos on skin surface pH, we performed the measurement of skin pH using skin pH meter PH900 in five healthy mixed breed dogs. The seven commercial shampoos: Humilac, Sebocalm, Sebolytics, Etiderm, PEroxyderm, HyLyt and Zn-7 Derm were included in this study. The anatomical sites, right thorax was the highest pH (7.66$\pm$0.10), and the lowest pH (6.20$\pm$0.23) was left pinna. A statiscally significant decrease in skin pH was found 7 minutes after application of Humilac, Sebocalm, Etiderm, Peroxyderm (p<0.01) and Sebolytics (p<0.05). After 17 minutes of application skin surface pH was inclined to increase in every shampoos but the degree of increase was slight at 77 minutes. No statiscally significant differences were found in HyLy-T and Zn-7 Derm, but skin pH was normal range (6.2-7.8) after application. Throughout the experiment skin surface pH was maintained above pH 7.0 in detergent. The commercial shampoos, Humilac, Sebocalm, Etiderm, had the decreasing effect on skin surface pH in dogs. The other four shampoos maintain the skin pH normal range. The skin pH meter PH 900 was found simple and useful for skin pH measurement.

• Journal of Korean Physical Therapy Science
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• v.10 no.1
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• pp.130-139
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• 2003

This study was conducted in order to determine the effect of Paraffin Therapy on release of pain, skin temperature and range of motion with joint diseases. This study was performed on 42 patients with general joint diseases and 21 patients with degenerative joint diseases. All the subjects were randomly assigned to three groups with 21 each. The experiment had been done from June, 1. 2002 to September, 30. 2002, in two general hospitals and a social welfare center using Digital Thermometer Kane-May KM330 and VAS(visual analogue scale). Each pint was measured in terms of skin temperature, pain scale and ROM(range of motion). The results of the experiment are as follows : 1. In application of three different heat modalities (Paraffin bath, Infra red, Hydro pack) on the Knee, Ankle, Elbow pints of the subjects for 30 minutes, the elevation of skin temperature and the reduction of pain scale were found to be significantly different. When checked right after the 30 minutes' application, the Infra red resulted in a higher degree elevation of skin temperature than two other modalities, while Paraffin bath resulted in the highest degree of the elevation of skin temperature when it was checked one hour after the application was over. It means that Paraffin bath lasted longer than the other two modalities(F=14.691, p<.000). 2. With Paraffin bath application, pint pain was reduced significantly both in 30 minutes and one hour checks(F=20.675, p<.001). 3. In terms of ROM(range of motion) increase, didn't seem to have any significant differences in 30 minutes and one hour. The results suggest that Paraffin therapy be helpful in maximizing th effects on pint diseases. I strongly suggest that further studies be done on other pints and muscle diseases using Paraffin bath in different methods of applying the therapy.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• v.9 no.1
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• pp.828-831
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• 2005

In this paper we propose a method to detect human faces from color image using pupil-template matching. Face detection is done by three stages. (i)separating skin regions from non-skin regions; (ii)generating a face regions by application of the best-fit ellipse; (iii)detecting face by pupil-template. Detecting skin regions is based on a skin color model. we generate a gray scale image from original image by the skin model. The gray scale image is segmented to separated skin regions from non-skin regions. Face region is generated by application of the best-fit ellipse is computed on the base of moments. Generated face regions are matched by pupil-template. And we detection face.

• Journal of the Korean Applied Science and Technology
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• v.26 no.2
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• pp.124-131
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• 2009

We have produced, characterized and compared different colloidal vehicles based on nanoemulsions. We also have investigated morphology and droplet distribution by means of electron microscope and photon correlation spectroscopy. Nanoemulsion systems characterized by different method on formulations have been obtained. Hydration power has been studied by means of a corneometer, measuring the skin electrical capacitance before and after the application of various type of skin lotions. It has been demonstrated that nanoemulsion with oil or fatty alcohol displayed a pronounced hydration power with respect to the solubilization system. In order to compare the smoothness of the skin after using skin lotion, we have measured the friction force. The skin lotions produced by nanoemulsion technique show improved smoothness of an atopic skin.