• Journal of Animal Reproduction and Biotechnology
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• v.39 no.1
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• pp.19-30
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• 2024

Background: Although an understanding of the proliferation and differentiation of fish female germline stem cells (GSCs) is very important, an appropriate threedimensional (3D) research model to study them is not well established. As a part of the development of stable 3D culture system for fish female GSCs, we conducted this study to establish a 3D aggregate culture system of ovarian cells in marine medaka, Oryzias dancena. Methods: Ovarian cells were separated by Percoll density gradient centrifugation and two different cell populations were cultured in suspension to form ovarian cell aggregates to find suitable cell populations for its formation. Ovarian cell aggregates formed from different cell populations were evaluated by histology and gene expression analyses. To evaluate the media supplements, ovarian cell aggregate culture was performed under different media conditions, and the morphology, viability, size, gene expression, histology, and E2 secretion of ovarian cell aggregates were analyzed. Results: Ovarian cell aggregates were able to be formed well under specific culture conditions that used ultra-low attachment 96 well plate, complete mESM2, and the cell populations from top to 50% layers after separation of ovarian cells. Moreover, they were able to maintain minimal ovarian function such as germ cell maintenance and E2 synthesis for a short period. Conclusions: We established basic conditions for the culture of O. dancena ovarian cell aggregates. Additional efforts will be required to further optimize the culture conditions so that the ovarian cell aggregates can retain the improved ovarian functions for a longer period of time.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.24 no.1
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• pp.43-53
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• 2011

For reinforced concrete members, the bond strength is one of the important factors between two materials: concrete and reinforcing element. This study concerns the bond strength of deformed bars in artificial lightweight aggregate concrete by pull-out test. 144 cubic specimens were manufactured for the test. concrete compressive strength, size of deformed bar and embedment lengths were considered as variables in this study. Normal concrete with W/C ratio 50% specimens were tested for the comparison. Test results included the bond stress-slip responses and modes of failure. Bond strength increased with an increase of compressive strength of concrete according to W/C ratio. The equation of bond stress of polymer-modified lightweight aggregate concrete were proposed by regression analysis based on the result.

• Resources Recycling
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• v.12 no.2
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• pp.11-20
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• 2003

Limestone mine waste was used as a aggregate far permeable concrete. Void ratio, continuous void ratio, coefficient of permeability, compressive strength and flexural strength of concrete were measured and then the relationship between porosity and strength properties was investigated. Void ratio, continuous void ratio and strength properties of permeable concrete were greatly influenced by the grain size of aggregate and void filling ratio in comparison with the containing ratio of limestone mine waste. Furthermore, void ratio showed a good relation with continuous void ratio, and porosity of permeable concrete indicated a good relation with strength properties also. The coefficient of permeability of permeable concrete using limestone waste was over 0.2 cm/sec and was excellent result in comparison with normal concrete. Therefore, it could be expected that the limestone mine waste would be utilized as aggregate for pavement concrete, green concrete and water resource specie concrete in the results of this study.

• Journal of the Korean Geosynthetics Society
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• v.6 no.2
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• pp.17-20
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• 2007

In this study, a series of uniaxial unconfined compression test and constant-head test were performed to investigate the mechanical characteristics of porous concrete using recycled-aggregate for the varying unit weight and water-cement ratio. To enhance the permeability of the porous concrete, the recycled-aggregate with similar grain size in the range of $40{\pm}5mm$ was used and water-cement ratio that leads to the lean-mix was adapted. The mechanical characteristics of the porous concrete cured for 3 days were examined; the compressive strength and $E_{50}$ showed their maximum values with 40% water-cement ratio and $1.8t/m^3$ unit weight and the permeability coefficient was averagely measured in the range of $0.9{\times}10^0cm/sec$ regardless of water-cement ratio and unit weight.

• Journal of the Korean Recycled Construction Resources Institute
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• v.10 no.3
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• pp.285-292
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• 2022

The objective of this study is to investigate whether CGS generated in IGCC satisfies the fine aggregate quality items specified in KS F 2527(Concrete Aggregate) through the pretreatment process system and the quality improvement the system. The statistical significance of the pretreatment process was analyzed through Repeated Measurements ANOVA as measured values according to individually pretreatment process system. As a result of the analysis, In the case of CGS fine aggregate quality before and after the pretreatment process system, the density increased 5.2 %, the absorption rate decreased by 1.86 %, the 0.08 mm passing ratio decreased by 2.25 %, and Fineness Modulus and Particle-size Distribution were also found to be adjustable. It was found that the pretreatment process system was significant in improving the quality of CGS.

• Journal of the Korea institute for structural maintenance and inspection
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• v.24 no.2
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• pp.68-77
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• 2020

The purpose of the paper is to evaluate the pore structure and mechanical properties of early frost damaged concrete using electric arc furnace slag as aggregate. From the results, when the concrete is exposed to frost damage at an early age, the peak point of pores 100 to 150 ㎛ in diameter were transferred into larger one. When the freezing duration is not exceeded 24 hours, it is possible that the pore distribution of under the 200 ㎛ is maintained and pore size of over 500 ㎛ is not formed, and, the freezing resistance of concrete using EFG could be improved. When BFS was mixed in concrete using EFG as coarse aggregate, the relative strength is higher than that of natural coarse aggregate. Meanwhile, the elastic modulus and resonance frequency did not change significantly due to the early frost damage as compared with the compressive strength. So, it is necessary to analyze the correlation between the experimental results in order to evaluate the performance degradation due to early frost damage.

• Journal of the Korea Concrete Institute
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• v.25 no.5
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• pp.477-484
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• 2013

In this study, eleven reinforced concrete beams, ground granulated blast furnace slag, replacing recycled coarse aggregate (BRS series) and recycled coarse aggregate with steel fiber (BSRS series), and standard specimen (BSS) were constructed and tested under monotonic loading. Experimental programs were carried out to improve and evaluate the shear performance of such test specimens, such as the load-displacement, the failure mode and the maximum load carrying capacity. All the specimens were modeled in 1/2 scale-down size. Test results showed that test specimens (BSRS Series) was increased the compressive strength by 9%, the maximum load carrying capacity by 1~6% and the ductility capacity by 1.02~1.13 times in comparison with the standard specimen (BSS). And the specimens (BSRS Series) showed enough ductile behavior and stable flexural failure.

• Journal of the Korea institute for structural maintenance and inspection
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• v.18 no.6
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• pp.1-10
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• 2014

In this study, thirteen reinforced concrete beams, ground granulated blast furnace slag, replacing recycled coarse aggregate with PVA fiber (BSPG series) and recycled coarse aggregate with hybrid fiber ($BSPGR_1$, $BSPGR_2$ series), and standard specimen (BSS) were constructed and tested under monotonic loading. Experimental programs were carried out to improve and evaluate the Structural performance of such test specimens, such as the load-displacement, the failure mode, and the maximum load carrying capacity. All the specimens were modeled in 1/2 scale-down size. Test results showed that test specimens ($BSPGR_1$, $BSPGR_2$ series) was increased the compressive strength by 13%, the maximum load carrying capacity by 4~21% and the ductility capacity by 4~28% in comparison with the standard specimen (BSS). And the specimens ($BSPGR_1$, $BSPGR_2$ series) showed enough ductile behavior and stable flexural failure.

• Journal of the Korean Geotechnical Society
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• v.31 no.1
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• pp.53-64
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• 2015

This study presents the experimental results of pervious blocks subjected to a series of unique inflow conditions in urban area. The measured properties include the strength, permeability, drainage capacity and runoff, and evaporation for blocks made of two different size of aggregates. Results revealed that the strength satisfies the Korean Standard regardless of aggregate size whereas the immediate runoff occurred for the block with small size aggregate. On the other hand, the block with large aggregates allowed the drainage upon the initial inflow condition, which became hampered to induce the runoff by subsequent inflow. It was attributed to the fact that the capillary water often served as the hydraulic barrier in partially saturated condition. The salient observation indicated that the runoff highly depended on the evaporation and pre-wetting condition as well as the porosity and pore connectivity. The bilinear evaporate rate that makes the degree of saturation vary also had great influence on deterining the time-dependent runoff.

• Journal of the Korean Society of Hazard Mitigation
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• v.9 no.1
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• pp.115-121
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• 2009

It is to use effectively for stream channel and watershed management as the prediction and the analysis of bed changes characteristics in the Seomjin river downstream. The necessary data (section, bed composition material, pivot point water elevation, coefficient of roughness) with regard to analysis of the bed changes characteristics were based upon the survey data and analysis results in the Seomjin river maintenance basic plan. The prediction of bed changes was also completed with HEC-6 model. The study results were summarized as follows: The main factor of bed changes in the Seomjin river downstream can be decided by extreme extraction of bed aggregate rather than the change of hydrological data. According to the analysis of bed stability based on the relation between friction velocity and representative grain size, and the relation between dimensionless tractive force and representative grain size, the Seomjin river downstream appears to be increased overall. The bed composition material in the stream channel of the Seomjin river of 2003 year shows higher composition rate of gravel and lower composition rate of sand as compared to those of 1989 year. According to result that the prediction of bed changes, it is estimated that the bed will be risen approximately 1.5 m to the place up to 9 km from the estuary, have been repetitively risen and fallen up to 1 m to the place between $9{\sim}21\;km$ section, and fallen about 0.5m to the place between $22{\sim}25\;km$ section. As a result, the bed of the Seomjin river downstream can be decided to be risen gradually. However, since the prediction of this study is based on the assumption that there will be no forced aggregate picking, the bed changes can be much greater than expected when there is a massive aggregate picking as it had happened before.