• Journal of Engineering Education Research
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• v.13 no.6
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• pp.33-48
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• 2010

This paper is aimed to design for qualification structure and criteria for preparation of qualification test of the environmental impact assessor qualifications. In this study, a DACUM(Development A curriculum) method job analysis to extract contents such as task range, eligibility, examination standards, selection criteria of examination questions, examination methods, etc was done by an job analysis committee composed of 9 specialists in the industry and academia. In the first place, this report presents job analysis models by procedures for developing selection criteria of examination questions of the environmental impact assessor. The first step is preparation for job analysis(data research, interviews and selection of experts), the second step the establishment of job models(first expert council and site study), the third step the drawn-out of knowledge, skills, and tools(the second expert council and site study), the fourth step the selection of courses and an examination subject(the third expert council), the fifth step the establishment of guidelines for making questions(the third site study).

• KSCE Journal of Civil and Environmental Engineering Research
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• v.26 no.3D
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• pp.385-391
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• 2006

It is very important to make a decision for locating public facility site in a objectively way people in general agree with. This is because public facility affects not only daily life of people in there but also a regional development. This paper sets up a site selection model which utilize an accessibility theory and GIS spatial analysis techniques. This model has been applied to all the twelve alternatives of Chungnam Provincial Office (CPO) sites which are well known to the public. On the criterion of average access time from all the other areas in Chungnam Province, CH alternative is found to be the best one followed by CH/AS, AS alternatives. On the basis of total people-travel time CH/AS alternative turns out to be the best one followed by CH, AS alternatives. In conclusion top three best locations for CPO are CH and AS area where transportation facility are in good condition and population density is highest in Chungnam Province. This fact implies that a transportation accessibility and population density are the primary key in determining the location of public facility.

• Korean Journal of Microbiology
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• v.50 no.1
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• pp.1-7
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• 2014

Site-specific incorporation of unnatural amino acids (SSIUA) into protein can be achieved in vivo by coexpression of an orthogonal pair of suppressor tRNA and engineered aminoacyl-tRNA synthetase (ARS) that specifically ligates an unnatural amino acid to the suppressor tRNA. As a step to develop the SSIUA technique in Escherichia coli, here we established a new 2-step screening system that can be used for selecting an ARS variant(s) that ligates an unnatural amino acid to a suppressor tRNA. A positive selection system consists of chloramphenicol acetyl transferase gene containing an amber mutation at the $27^{th}$ residue, and efficiently concentrated amber suppressible ARS with a maximum enrichment factor of $9.0{\times}10^5$. On the other hand, a negative selection system was constructed by adding multiple amber codons in front of a lethal gene encoding the control of cell death B toxin (ccdB) which acts as an inhibitory protein of bacterial topoisomerase II. Amber suppression of ccdB by an orthogonal pair of Saccharomyces cerevisiae tyrosyl-tRNA synthetase (TyrRS) and an amber suppressor tRNA significantly inhibits bacterial growth. This selection system was also able to efficiently remove amber suppressible ARS which could ligate natural amino acids to the suppressor tRNA. Thus, sequential combination of these two selection systems might be able to function as a powerful tool for selecting an ARS variant that specifically ligates an unnatural amino acid to the suppressor tRNA from an ARS mutant pool.

• Proceedings of the KSR Conference
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• 2007.11a
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• pp.595-600
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• 2007

Greater expansion and more frequent operation of the railroad transportation system anticipated due to its characteristics including low cost, safety and mass transportation. Recently, effects on the railway structures due to expansion of newly constructed road, construction of subway, city gas pipeline, communication network, electric power network and construction of other railway underground crossing in accordance with urban planning and organization has influenced safe operation of trains. Accordingly, standard for selection of construction method that will enable construction of more economical and rational subway underground crossing structures by preventing problems occurring at the time of above construction works and accidents in safe operation of trains due to construction in advance is definitively necessary. Although there are numerous construction methods that can be applied at the time of construction of railway underground crossing, there are much difficulties in selection of appropriate construction method that considers characteristics of each construction method on non-excavation type construction method, train operation plan of number of operational routes and on-site circumstances. Therefore, this research aims to present rational standard for selection of construction method for such, and standard for slowdown speed and interception of train when passing the areas of slowdown in sectors under construction.

• Proceedings of the KAIS Fall Conference
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• 2006.05a
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• pp.608-610
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• 2006

Supply Chain Management(SCM) system is a critical investment that can affect future competitiveness and performance of a company. When adopting a new SCM system, organizations experience increasing difficulty in decision making because information technology is changing so rapidly these days. Therefore, organizations have been looking for industry standards and proven methods of selection that they can utilize to choose the best SCM system. To select an optimum solution, we need to consider a number of different quantitative and qualitative factors such as cost, user interface and convenience, reference site, and so on. In this study, we propose a solution selection model of SCM systems using Fuzzy AHP to maximize the return on investment in information technology. The proposed model can systematically construct the objectives of SCM system selection to support the business goals.

• Proceeding of Spring/Autumn Annual Conference of KHA
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• 2002.11a
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• pp.295-300
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• 2002

Since the contents of large-scale repair of a condominium were various, a plan document was not prepared. The owners association of the condominium has given priority to decision-making of price priority over quality. Examination is not performed about the contents of the construction supervision business, which checks whether suitable construction is performed, either. On the other hand, the know-how for builders, such as decision of the process plan under which the repair work lessens influence to the safety and life as much as possible since the place where the resident is living turns into a construction site, and selection of a method of construction, is just going to ask. It is an important subject how the structure of the construction contractor selection, which considered quality, is built. Then, this research focuses on large-scale repair construction of the condominium, and arranges the actual condition of a series of processes from plan document creation to construction supervision. The method of research collects the construction documents of the condominium that carried out Barge-scale repair construction. Next, collection arrangement of the data of construction builder selection is carried out. The method of the construction builder selection of those other than a construction price is examined. The method of quality control of large-scale repair construction is examined.

• Journal of the Korea Institute of Building Construction
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• v.16 no.4
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• pp.331-339
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• 2016

According to high-rise, complexation, and enlargement of buildings, various construction methods are being developed, and the significance of construction method selection about main work types has emerged as a major interest. However, it has been pointed out that hand-on workers cannot consider project characteristics carefully, and they lack an objective standard or reference for main construction method selection. Hence, the selection is being made depending on hand-on workers' experience and intuition. To solve this problem, various studies have proceeded for construction method selection of main work types using Artificial Intelligence like Fuzzy, AHP and Case-based reasoning. It is difficult to apply many different kinds of construction method selection to every main work type with consideration for characteristics of work types and condition of a construction site when selecting construction method in the field. Accordingly, this study proposed the decision-making model which can apply to fields easily. Using matrix analysis and liner transformation, this study verified consistency of study models applied in the process of soil retaining selection with a case study.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.20 no.9
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• pp.1696-1703
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• 2016

In this paper, it verifies site environment aspect that NDGPS (Nationwide Differential Global Positioning System) operated by MOF (Ministry of Oceans and Fisheries) will be used as the same site of reference stations for SBAS (Satellite Based Augmentation System). In order to prove this feasibility, we analyze the site environment requirements for SBAS reference stations, as well as we establish the procedure for the verification of the site environment requirements. With this procedure of the site environment survey, we perform site survey in the real field and analyze the results. We select interim candidate sites for survey which currently operating 17 NDGPS reference stations. This paper could be utilized in the process of selection or installation of reference stations in the field of GNSS(Global Navigation Satellite System) and the drawing the consideration which NDGPS reference stations will be co-operated as SBAS reference stations.

• Journal of Microbiology and Biotechnology
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• v.16 no.5
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• pp.704-715
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• 2006

Oil spill was found in 1999 from a diesel storage facility located near the top of Baekun Mountain in Uiwang City. Application of bioremediation techniques was very relevant in removing oil spills in this site, because the geological condition was not amenable for other onsite remediation techniques. For efficient bioremediation, bacterial communities of the contaminated site and the uncontaminated control site were compared using both molecular and cultivation techniques. Soil bacterial populations were observed to be stimulated to grow in the soils contaminated with diesel hydrocarbon, whereas fungal and actinomycetes populations were decreased by diesel contamination. Most of the dieseldegrading bacteria isolated from contaminated forest soils were strains of Pseudomonas, Ralstonia, and Rhodococcus species. Denaturing gradient gel electrophoresis (DGGE) analysis revealed that the profiles were different among the three contaminated sites, whereas those of the control sites were identical to each other. Analysis of 16S rDNA sequences of dominant isolates and clones showed that the bacterial community was less diverse in the oil-contaminated site than at the control site. Sequence analysis of the alkane hydroxylase genes cloned from soil microbial DNAs indicated that their diversity and distribution were different between the contaminated site and the control site. The results indicated that diesel contamination exerted a strong selection on the indigenous microbial community in the contaminated site, leading to predominance of well-adapted microorganisms in concurrence with decrease of microbial diversity.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2001.06a
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• pp.35-39
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• 2001

The Integration Host factor (IHF) of Escherichia coli is a small, basic protein that is required for a variety of functions including site-specific recombination, transposition, gene regulation, plasmid replication, and DNA packaging. It ,is composed of two subunits that are encoded by the ihfA ($\alpha$-subunit) and ihjB ($\beta$-subunit) genes. IHF binding sites are composed of three elements called the WATCAR, TTG, and poly (dAT) elements. We have characterized IHF binding to the H site of bacteriophage λ. We have isolated suppressors that bind to altered H' sites using a challenge phage selection. Two different suppressors were isolated that changed the adjacent $\alpha$P64 and $\alpha$K65 residues. The suppressors recognized both the wild-type site and a site with a change in the WATCAR element. Three suppressors were isolated at $\beta$-E44. These suppressors bound the wild-type and a mutant site with a T：A to A：T change (H44A) in the middle of the TIR element. Site-directed mutagenesis was used to make several additional changes at $\beta$E44. The wild-type and $\beta$E44D mutant could not bind the wild-type site but were able to bind the H44A mutant site. Other mutants with neutral, polar, or a positive charge at $\beta$E44 were able to repress both the wild-type and H44A sites. Examination of the IHF crystal structure suggests that the ability of the wild-type and $\beta$E44D proteins to discriminate between the T：A and A：T basepairs is due to indirect interactions. The $\beta$-E44 residue does not contact the DNA directly. It imposes binding specificity indirectly by interactions with residues that contact the DNA. Details of the proposed interactions are discussed.