• BMB Reports
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• v.29 no.1
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• pp.17-21
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• 1996

Ile91 of restriction endonuclease EcoRV, which has not been known to take part directly in catalytic activity, was substituted with Leu by site-directed mutagenesis. The Ile91Leu mutant shows over 1000-fold less activity than the wild type EcoRV under standard reaction condition. The metal ion dependency of the reaction was altered. In contrast to the wild type EcoRV, the mutant prefers $Mn^{2+}$ to $Mn^{2+}$ as the cofactor. In $Mn^{2+}$ buffer the mutant is as active as the wild type enzyme in $Mn^{2+}$ buffer. Like the wild type enzyme, the mutant shows an unspecific binding of DNA in gel shift experiments. In contrast to the wild type enzyme, the mutant did not cleave at noncognate sites of DNA under star condition.

• Journal of the Korea Institute of Building Construction
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• v.2 no.4
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• pp.137-144
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• 2002

In the modern age. Building is getting higher and larger according to owner and social demands. Now when we are facing the need of the cost down and the reduction of economic loss. In this paper, the characteristics of the present conditions of site management were investigated with the field survey. And The Purpose of this study is to suggest the rational plans of construction planning in site management. The method of site management. however depends on experience of a construction manager. in order to solve this situation, it is necessary to create a method of site management that is adequate for the condition of construction. By cultivating excellent sub contractors, we can expect precise construction with a few experts. improvement in qualify of construction from standardization, computerization of each process. And the expectancies of this paper are that it can be used as efficient data for improvement of system to systematize site management work in Korea.

• Journal of the Korea Institute of Building Construction
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• v.5 no.3 s.17
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• pp.91-99
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• 2005

It is required to study systematical on the concrete quality management to extend structure life because rebuilding effect is reducing under present condition of large sized and high stories structure. Concrete, which shows its intensity by hydrating action and a big change in quality according to hot weather and temperature, produces a lot of qualify problem under hot and cold weather Because of each specification and construction plan which does not have basic standard on site, concrete's quality is irregular and makes some defect. As a result, Daegu is turned out to be the longest area after investigating application period and days focused on 8 cities weather information about relationship between hot weather circumstance and construction environment. Therefore, we first surveyed the concrete material in the region and found out the problem of quality nanagement. Then figure out the way of solution. Moreover, we integrated concrete material quality management, which is applied differently to each site, to have equal quality and to reduce defect from construction site. And then, based on various test of construction condition and analysis of quality management item, we suggest effective concrete quality management to make concrete material construction standard guide and plan under hot weather.

• Proceedings of the Korean Nuclear Society Conference
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• 2004.10a
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• pp.1381-1382
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• 2004

• Knowledge Management Research
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• v.10 no.2
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• pp.49-63
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• 2009

In this study, sub domain of flow was investigated on literature survey, and suggested of the measuring method of web-site flow and its simulation analysis. Constructing of measuring method of flow, and using this method what-if analysis was simulated when several condition changed. Using causal map approach to extract knowledge from web-site domain experts and to derives a causal relationship of knowledge. Specially, in our study, describes method of developing and building causal map, and suggests guide line of this method on practical application. This research results show that web-site flow starts "direct searching" or "interesting of special issue(domain)", and when challenges of web-site were accorded with user's skills web-site flow grows. Further, in the web-site, information searching intention results in increase of user's duration time and experience flow to discovery new interesting issues in this process. If user's web-site of interaction is increased, awareness of environment conditions decreased, finally, user's telepresense results in increased web-site flow. This paper contained thai this method make used of measuring flow in the web-site and developing of practical strategy.

• Journal of architectural history
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• v.25 no.1
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• pp.7-21
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• 2016

The purpose of this paper is to study on the characteristic of site planning of Hadrian's villa. The whole site planning of Hadrian's villa can be divided by different characteristic four groups(I-IV). It is analysed to the whole site plan of such huge complex and the site planning of partial each group which is organically connected each other. The results of this study are as follows: 1. Hadrian's villa was arranged in adapt to natural condition according to a gentle inclination by topography and terrain of the whole site which many different buildings are oriented towards natural landscape of the site and the site surroundings. 2. The site planning of Hadrian's villa is composed of dividing into four groups as plan segmentalized through various land use plan, considering for territory of functional aspect and direction of axis formed by situation and direction of topography and terrain. 3. The concept of site planning to buildings of Hadrian's villa is composed through each other connection; the first, meeting, crossing and merging with each other from arrangement by several axises, the second, change of direction with as the center a part of corner of buildings and the third, facing or inserting system to side and side, side and a part of corner of building.

• Geomechanics and Engineering
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• v.16 no.4
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• pp.435-448
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• 2018

In order to make reliable earthquake-resistant design of civil engineering structures, one of the most important considerations in a region with high seismicity is to pay attention to the local soil condition of regions. It is aimed in the current study at specifying dynamic soil characteristics of Kirikkale city center conducting the 1-D equivalent linear and non-linear site response analyses. Due to high vulnerability and seismicity of the city center of Kirikkale surrounded by active many faults, such as the North Anatolian Fault (NAF), the city of Kirikkale is classified as highly earthquake-prone city. The first effort to determine critical site response parameter is to perform the seismic hazard analyses of the region through the earthquake record catalogues. The moment magnitude of the city center is obtained as $M_w=7.0$ according to the recorded probability of exceedance of 10% in the last 50 years. Using the data from site tests, the 1-D equivalent linear (EL) and nonlinear site response analyses (NL) are performed with respect to the shear modulus reduction and damping ratio models proposed in literature. The important engineering parameters of the amplification ratio, predominant site period, peak ground acceleration (PGA) and spectral acceleration values are predicted. Except for the periods between the period of T=0.2-1.0 s, the results from the NL are obtained to be similar to the EL results. Lower spectral acceleration values are estimated in the locations of the city where the higher amplification ratio is attained or vice-versa. Construction of high-rise buildings with modal periods higher than T=1.0 s are obtained to be suitable for the city of Kirikkale. The buildings at the city center are recommended to be assessed with street survey rapid structural evaluation methods so as to mitigate seismic damages. The obtained contour maps in this study are estimated to be effective for visually characterizing the city in terms of the considered parameters.

• Journal of Environmental Science International
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• v.8 no.3
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• pp.325-330
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• 1999

This study aims to observe the inhibition of methane generation, the decomposition of organic matter, and the trend of outflowing leachate, using the simulated column of the anaerobic sanitary landfill structure of sulfate addition type which is made by adding sulfate to a current anaerobic landfill structure, and the simulated column of semi-aerobic landfill structure in the laboratory which is used in the country like Japan in order to inhibit methane from a landfill site among the gases caused by a global warming these days, and at the same time to promote the decomposition of organic matter, the index of stabilization of landfill site. As a result of this study, it is thought that the ORP(Oxidation Reduction Potential) of the column of semi-aerobic landfill structure gradually represents a weak aerobic condition as time goes by, and that the inside of landfill site is likely to by in progress into anaerobic condition, unless air effectively comes into a semi-aerobic landfill structure in reality as time goes by. In addition, it can be seen that the decomposition of organic matter is promoted according to sulfate reduction in case of $R_1$, a sulfate-added anaerobic sanitary landfill structure, and that the stable decomposition of organic matter in $R_1$ makes a faster progess than $R_2$. Moreover it can be estimated that $R_1$, a sulfate-added anaerobic sanitary landfill structure has an inhibition efficiency of 55% or so, compared with $R_2$, a semi-aerobic landfill structure, in the efficiency of inhibiting methane.

• 한국신재생에너지학회:학술대회논문집
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• 2008.05a
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• pp.138-141
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• 2008

The effects of design parameters for small scale hydro power(SSHP) plants due to rainfall condition have been studied. The model to predict hydrologic performance for SSHP plants is used in this study. The results from analysis for rainfall conditions based on Weibull distribution show that the capacity and load factor of SSHP site had large difference between the variation of shape and scale parameter. Especially, the hydrologic performance of SSHP site due to variation of shape parameter varied more sensitive than the case of variation of scale parameter. And also, the methodology represented in this study can be used to decide the primary design specifications of SSHP sites.

• Journal of the Korean Solar Energy Society
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• v.29 no.6
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• pp.56-61
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• 2009

The effects of design parameters for small scale hydro power(SSHP) plants due to climate change have been studied. The model to predict hydrologic performance for SSHP plants is used in this study. The results from analysis far rainfall conditions based on KIER model show that the capacity and load factor of SSHP site had large difference between the period. Especially, the hydrologic performance of SSHP site due to rainfall condition of recent period varied in design flowrate sensitively. However climate change gave small effect in load factor of existing SSHP plant. And also, the methodology represented in this study can be used to decide the primary design specifications of SSHP sites.