• BMB Reports
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• v.54 no.2
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• pp.98-105
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• 2021

The clustered regularly interspaced short palindromic repeats (CRISPR) system is a family of DNA sequences originally discovered as a type of acquired immunity in prokaryotes such as bacteria and archaea. In many CRISPR systems, the functional ribonucleoproteins (RNPs) are composed of CRISPR protein and guide RNAs. They selectively bind and cleave specific target DNAs or RNAs, based on sequences complementary to the guide RNA. The specific targeted cleavage of the nucleic acids by CRISPR has been broadly utilized in genome editing methods. In the process of genome editing of eukaryotic cells, CRISPR-mediated DNA double-strand breaks (DSB) at specific genomic loci activate the endogenous DNA repair systems and induce mutations at the target sites with high efficiencies. Two of the major endogenous DNA repair machineries are non-homologous end joining (NHEJ) and homology-directed repair (HDR). In case of DSB, the two repair pathways operate in competition, resulting in several possible outcomes including deletions, insertions, and substitutions. Due to the inherent stochasticity of DSB-based genome editing methods, it was difficult to achieve defined single-base changes without unanticipated random mutation patterns. In order to overcome the heterogeneity in DSB-mediated genome editing, novel methods have been developed to incorporate precise single-base level changes without inducing DSB. The approaches utilized catalytically compromised CRISPR in conjunction with base-modifying enzymes and DNA polymerases, to accomplish highly efficient and precise genome editing of single and multiple bases. In this review, we introduce some of the advances in single-base level CRISPR genome editing methods and their applications.

• Radiation Oncology Journal
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• v.34 no.4
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• pp.313-321
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• 2016

Purpose: Total scalp irradiation (TSI) is a rare but challenging indication. We previously reported that non-coplanar intensity-modulated radiotherapy (IMRT) was superior to coplanar IMRT in organ-at-risk (OAR) protection and target dose distribution. This consecutive treatment planning study compared IMRT with volumetric-modulated arc therapy (VMAT). Materials and Methods: A retrospective treatment plan databank search was performed and 5 patient cases were randomly selected. Cranial imaging was restored from the initial planning computed tomography (CT) and target volumes and OAR were redelineated. For each patients, three treatment plans were calculated (coplanar/non-coplanar IMRT, VMAT; prescribed dose 50 Gy, single dose 2 Gy). Conformity, homogeneity and dose volume histograms were used for plan. Results: VMAT featured the lowest monitor units and the sharpest dose gradient (1.6 Gy/mm). Planning target volume (PTV) coverage and homogeneity was better in VMAT (coverage, 0.95; homogeneity index [HI], 0.118) compared to IMRT (coverage, 0.94; HI, 0.119) but coplanar IMRT produced the most conformal plans (conformity index [CI], 0.43). Minimum PTV dose range was 66.8%-88.4% in coplanar, 77.5%-88.2% in non-coplanar IMRT and 82.8%-90.3% in VMAT. Mean dose to the brain, brain stem, optic system (maximum dose) and lenses were 18.6, 13.2, 9.1, and 5.2 Gy for VMAT, 21.9, 13.4, 14.5, and 6.3 Gy for non-coplanar and 22.8, 16.5, 11.5, and 5.9 Gy for coplanar IMRT. Maximum optic chiasm dose was 7.7, 8.4, and 11.1 Gy (non-coplanar IMRT, VMAT, and coplanar IMRT). Conclusion: Target coverage, homogeneity and OAR protection, was slightly superior in VMAT plans which also produced the sharpest dose gradient towards healthy tissue.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.12
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• pp.8908-8914
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• 2015

The Internet continues to grow and develop, but there are going to generate a variety of Internet attacks that exploit it. In the initial Internet environment, the attackers maliciously exploited Internet environments for ostentations and hobbies. but these days many malicious attempts purpose the financial gain so systematic and sophisticated attacks that are associated with various crimes are occurred. The structures, such as viruses and worms were present in the form of one source multi-target before. but recently, APT(Advanced Persistent Threat, intelligent continuous attacks) in the form of multi-source single target is dealing massive damage. The performance evaluation analyzed whether to generate audit data and detect integrity infringement, and false positives for normal traffic, process detecting and blocking functions, and Agent policy capabilities with respect to the application availability.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.44 no.1
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• pp.68-75
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• 2007

A single chip NFC transceiver supporting not only NFC active and passive mode but also 13.56MHz RFID reader and tag mode was designed and fabricated. The proposed NFC transceiver can operate as a RFID tag even without external power supply which has dual antenna structure for initiator and target. The area increment due to additional target antenna is negligible because the target antenna is constructed by using a shielding layer of initiator antenna. The analog front end circuit of the proposed NFC transceiver consists of a transmitter and receiver of reader/writer block supporting NFC initiator or RFID reader mode, and a tag circuit for target of passive NFC mode or RFID tag mode. The maximum baud rate of the proposed NFC device is 212kbps by using UART serial interface. The chip has been designed and fabricated using a Magnachip's $0.35{\mu}m$ double poly 4-metal CMOS process, and the effective area of the chip is 2200um by 3600um.

• Journal of the Korean institute of surface engineering
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• v.27 no.2
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• pp.65-73
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• 1994

Effects of deposition parameter on the ionic conductivity and structural change of the Lithium borosili-cate solid electrolyte films, prepared by rf sputtering using 7$LI_2O-3B_2O_3-1SiO_2$ single phase target and also a mosaic target enriched with $LI_2O$, were analyzed by measuring AC impedance and IR absorption spectra for the films. Thed solid electrolyte film deposited from the single phase target exhibited very low ionic conductivi-ty of $10^{-10}{\Omega}^{-1}cm{-1}$ at room temperature, a result of low $Li^+$ ion content(7.52 at%) in the film. The $Li^+$ con-ductivity for the films deposited from the mosaic target, however, significantly increased to $10^{-7}{\Omega}^{-1}cm{-1}$ due to both an increased $Li^+$content (14.75 at %) and a structural change of the films. The increased ionic conduc-tivity of the film appears to be associated with an easiness of ionic mobility by structural change of glassy film from a some close packed network structure to a open one. These structural changes of film were found to be closely related to the increase in the peak intensity at~$960cm^{-1}$ of IR absorption spectra for the glassy films. With increasing either argon pressure from 3 to 21 mtorr or rf power from 2 to 3 W/$cm^2$, the $Li^+$ conduc-tivity for the films significantly increased to an order of $10^{-6}{\Omega}^{-1}cm{-1}$ due to an increase in openness of film structure, as confirmed by both an increase in the IR absorption peak intensity at ~$960cm^{-1}$ and a resultant reduction of activation energy for mobility of $Li^+$ ion.

• Radiation Oncology Journal
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• v.9 no.2
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• pp.351-359
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• 1991

The current LINAC technique for radiosurgery utilizes a single isocenter approach with multiple noncoplanar arcs. This approach results in spherical dose distributions in the target. Many arteriovenous malformations and tumors suitable for radiosurgical treatment have non-spherical or irregular shapes. The basic approach presented in this paper is to use two or multiple isocenters with standard arcs to shape irregular target volumes through the use of multiple spherical targets. Selection of reasonable irradiation parameters in the first stage is critical to the success of real-time optimization. A useful guideline for optimum isocenter separation and collimator size is developed to shape the target margin uniformly with an desired isodose surface for an elongated target. The implementation of multiple isocenters with three dimensional dose model and application of multiple isocenters approach to several cases are discussed.

• Journal of Korea Multimedia Society
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• v.25 no.10
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• pp.1375-1385
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• 2022

In this paper, we propose a semi-supervised domain adaptation solution to deal with practical face recognition (FR) scenarios where a single face image for each target identity (to be recognized) is only available in the training phase. Main goal of the proposed method is to reduce the discrepancy between the target and the source domain face images, which ultimately improves FR performances. The proposed method is based on the Domain Adatation network (DAN) using an MMD loss function to reduce the discrepancy between domains. In order to train more effectively, we develop a novel loss function learning strategy in which MMD loss and cross-entropy loss functions are adopted by using different weights according to the progress of each epoch during the learning. The proposed weight adoptation focuses on the training of the source domain in the initial learning phase to learn facial feature information such as eyes, nose, and mouth. After the initial learning is completed, the resulting feature information is used to training a deep network using the target domain images. To evaluate the effectiveness of the proposed method, FR performances were evaluated with pretrained model trained only with CASIA-webface (source images) and fine-tuned model trained only with FERET's gallery (target images) under the same FR scenarios. The experimental results showed that the proposed semi-supervised domain adaptation can be improved by 24.78% compared to the pre-trained model and 28.42% compared to the fine-tuned model. In addition, the proposed method outperformed other state-of-the-arts domain adaptation approaches by 9.41%.

• Journal of the Institute of Convergence Signal Processing
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• v.11 no.3
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• pp.203-208
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• 2010

In this paper, we convert underwater acoustic signal made by sonar system into digital image. We propose the algorithm that detects target candidate and emphasizes information of target introducing image processing technique for the digital image. The process detecting underwater target estimates background noise in underwater acoustic signal changing irregularly, recomposes it. and eliminates background from original image. Therefore, it generates initial target group. Also, it generates weighted map through proceeding doppler information, ensures information for target candidate through filtering using weighted map for image eliminated background noise, and decides the target candidate area in the single frame. In this paper, we verified that proposed algorithm almost had eliminated the noise generated irregularly in underwater acoustic signal made by simulation, targets had been displayed more surely in the image of underwater acoustic signal through filtering and process of target detection.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.12 no.4
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• pp.29-33
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• 2013

Here we report a partially aminated micropattern via direct functionalization and examine eleven different solution-phase probe DNAs hybridizing with the same target DNA on both hydrogen and oxygen terminated diamond. The hybridization intensities determined by epifluorescence microscopy were compared and are influenced strongly by the negatively charged surface and mismatched position of its sequence with immobilized probe DNA.

• 제어로봇시스템학회:학술대회논문집
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• 1991.10a
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• pp.687-692
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• 1991

To reduce the complexity in constructing a fuzzy logic controller of multivariable systems, three major methods are presented. One is the method of constructing single-input-single-output fuzzy logic controllers after decoupling the target system. Another is the method of using fuzzy relation matrices which indicate the relation between each input and output. The other is the method of using the hierarchically classified inputs which dominantly influence one output than other inputs. Using the last two methods, simulation results of fuzzy logic controller implemented on 160MW boiler-turbine plant model are also shown.