• The Transactions of the Korean Institute of Electrical Engineers C
• /
• v.53 no.5
• /
• pp.286-292
• /
• 2004

In this study, an integrated microelectrode array was fabricated on glass slide using microfabrication technology. Probe DNAs consisting of mercaptohexyl moiety at their 5-end were spotted on the gold electrode using micropipette or DNA arrayer utilizing the affinity between gold and sulfur. Cyclic voltammetry in 5mM ferricyanide/ferrocyanide solution at 100 ㎷/s confirmed the immobilization of probe DNA on the gold electrodes. When several DNAs were detected electrochemically, there was a difference between target DNA and control DNA in the anodic peak current values. It was derived from specific binding of Hoechst 33258 to the double stranded DNA due to hybridization of target DNA. It suggested that this DNA chip could recognize the sequence specific genes. It suggested that multichannel electrochemical DNA microarray is useful to develop a portable device for clinical gene diagnostic System.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
• /
• v.29 no.11
• /
• pp.1-5
• /
• 2015

In this paper, we propose the optical system design for a medium sized LED marine lanterns which simplifies the multi-layer structure into a single structure. In order to satisfy the target fixed intensity(35,000cd) and vertical divergence($-2.5^{\circ}{\sim}-4.0^{\circ}$, $+2.5^{\circ}{\sim}+4.0^{\circ}$), we use the total internal reflection collimator lens. And a Monte Carlo simulation has been utilized to optimize a condition of a LED package, TIR lens and outside lens. The computer simulation results indicated that this LED marine lanterns can produce of a fixed intensity(35,382cd) and vertical divergence($-3.1^{\circ}{\sim}+2.5^{\circ}$). Using the this optical system, we achieve the target value of LED lanterns.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2000.07a
• /
• pp.243-246
• /
• 2000

The sputtering systems mainly consist of the three-target holder. The target and substrate were the on-axis type. The MgO and STO substrate were used for the deposition of each layer. The optimum conditions of single-layer thin film were investigated from the SEM images and the XRD patterns. Based on the above conditions, the multi-layer thin films such as YBaCuO/LaGaO/Au/Nb and YBaCuO/Au/Nb were fabricated. The crystalline, the electrical Properties, the energy gap structure and the characteristics of the tunneling barrier on the multi-layer thin film have been investigated and characterized.

• 제어로봇시스템학회:학술대회논문집
• /
• 2001.10a
• /
• pp.70.1-70
• /
• 2001

Recently, research of visual control is getting more essential in robotic application, and acquiring 3D informations from the 2D images is becoming more important with development of vision system. For this application, we propose the effective way of controlling stereo vision tracking system for target tracking and calculating distance between target and camera. In this paper we address improved controller using dual-loop visual servo which is more effective compared with using single-loop visual servo for stereo vision tracking system. The speed and the accuracy for realizing a real time tracking are important. However, the vision processing speed is too slow to track object in real time by using only vision feedback data. So we use another feedback data from controller parts which offer state feedback ...

• Journal of Industrial Convergence
• /
• v.2 no.1
• /
• pp.121-137
• /
• 2004

Is entering in age that brand becomes consumer's goods purchase standard as income level is improved, dying injunction brand is trend that governing power in the world market is expanded as international economy does single market. Situation to develop Kyonggi Province area to universal tourist attraction area keeping in step with national large size event holding such as sun of Korean visit with opening a port of the In-chon International airport, the world ceramics Expo, World Cup was made up. Growth possibility is big to culture tourism resources and representative tourist resort of the Korea that use view of nature photon circle properly such as ceramics that target area possesses. Area development plan through growth possibility should be arranged to international ceramics production complex and distribution complex. Therefore, plan analyzing future district theme establishment and strength in priority via past and present of target area.

• Journal of Microbiology and Biotechnology
• /
• v.31 no.7
• /
• pp.903-911
• /
• 2021

Previous studies have modified microbial genomes by introducing gene cassettes containing selectable markers and homologous DNA fragments. However, this requires several steps including homologous recombination and excision of unnecessary DNA regions, such as selectable markers from the modified genome. Further, genomic manipulation often leaves scars and traces that interfere with downstream iterative genome engineering. A decade ago, the CRISPR/Cas system (also known as the bacterial adaptive immune system) revolutionized genome editing technology. Among the various CRISPR nucleases of numerous bacteria and archaea, the Cas9 and Cas12a (Cpf1) systems have been largely adopted for genome editing in all living organisms due to their simplicity, as they consist of a single polypeptide nuclease with a target-recognizing RNA. However, accurate and fine-tuned genome editing remains challenging due to mismatch tolerance and protospacer adjacent motif (PAM)-dependent target recognition. Therefore, this review describes how to overcome the aforementioned hurdles, which especially affect genome editing in higher organisms. Additionally, the biological significance of CRISPR-mediated microbial genome editing is discussed, and future research and development directions are also proposed.

• Journal of Korea Multimedia Society
• /
• v.25 no.6
• /
• pp.819-832
• /
• 2022

Cross-domain recommender system transfers knowledge across different domains to improve the recommendation performance in a target domain that has a relatively sparse model. However, they suffer from the "negative transfer" in which transferred knowledge operates as noise. This paper proposes a novel Multiple Fusion-based Deep Cross-Domain Recommendation named MFDCR. We exploit Doc2Vec, one of the famous word embedding techniques, to fuse data user-wise and transfer knowledge across multi-domains. It alleviates the "negative transfer" problem. Additionally, we introduce a simple multi-layer perception to learn the user-item interactions and predict the possibility of preferring items by users. Extensive experiments with three domain datasets from one of the most famous services Amazon demonstrate that MFDCR outperforms recent single and cross-domain recommendation algorithms. Furthermore, experimental results show that MFDCR can address the problem of "negative transfer" and improve recommendation performance for multiple domains simultaneously. In addition, we show that our approach is efficient in extending toward more domains.

• Journal of Korean Ophthalmic Optics Society
• /
• v.20 no.2
• /
• pp.177-186
• /
• 2015

Purpose: The Purpose of this study is to investigate if the relative legibility of optotypes affects the corrected visual acuity in visual acuity test. Methods: After measuring the relative legibility of 'Landolt ring target', 'arabic number target', 'alphabet target' by showing as a single-letter-target in 24 subjects without specific ocular diseases and ocular surgery experience, the relative legibility of 0.8, 1.0, 1.25 row of vision according to type of target in 7 types of chart were compared. After then we compared by measuring the corrected visual acuity according to type of target by using binocular MPMVA test (#7A) in 60 myopic subjects. Results: In 3 types of target the worst relative legibility target was 'Landolt ring target' with legible distance of $98.97{\pm}4.57cm$ and the best relative legibility target was 'alphabet target' with legible distance of $108.42{\pm}3.46cm$. There was no difference of the relative legibility according to type of chart or visual acuity level in the row of vision if other conditions are the same. In 1.0 and 1.25 row of vision the difference of relative legibility according to type of target was shown the statistically significant difference between 'Landolt ring target' and 'alphabet target' as $-0.07{\pm}0.06$ (p=0.02) and $-0.06{\pm}0.06$ (p=0.04) respectively. In myopia the difference of corrected visual acuity according to type of target was statistically significant difference between 'Landolt ring target' and 'arabic number target' as $-0.04{\pm}0.02$ (p=0.02) and it was especially remarkable in the low myopia. Conclusions: Measuring visual acuity with different optotypes could cause the errors in best vision measurement value because there was difference of the relative legibility according to type of target even though visual acuity level is same in the row of vision.

• Asian-Australasian Journal of Animal Sciences
• /
• v.33 no.9
• /
• pp.1400-1410
• /
• 2020

Objective: Genome-wide association study and two meta-analysis based on GWAS performed to explore the genetic mechanism underlying variation in pig number born alive (NBA) and total number born (TNB). Methods: Single trait GWAS and two meta-analysis (single-trait meta analysis and multi-trait meta analysis) were used in our study for NBA and TNB on 3,121 Yorkshires from 4 populations, including three different American Yorkshire populations (n = 2,247) and one British Yorkshire populations (n = 874). Results: The result of single trait GWAS showed that no significant associated single nucleotide polymorphisms (SNPs) were identified. Using single-trait meta analysis and multi-trait meta analysis within populations, 11 significant loci were identified associated with target traits. Spindlin 1, vascular endothelial growth factor A, forkhead box Q1, msh homeobox 1, and LHFPL tetraspan submily member 3 are five functionally plausible candidate genes for NBA and TNB. Compared to the single population GWAS, single-trait Meta analysis can improve the detection power to identify SNPs by integrating information of multiple populations. The multiple-trait analysis reduced the power to detect trait-specific loci but enhanced the power to identify the common loci across traits. Conclusion: In total, our findings identified novel genes to be validated as candidates for NBA and TNB in pigs. Also, it enabled us to enlarge population size by including multiple populations with different genetic backgrounds and increase the power of GWAS by using meta analysis.

• Journal of Advanced Marine Engineering and Technology
• /
• v.30 no.5
• /
• pp.572-579
• /
• 2006

Accurate heat release analysis based on the cylinder pressure trace is important for evaluating combustion process of diesel engines. However, traditional single-zone heat release models (SZM) have significant limitations due mainly to their simplified assumptions of uniform charge and homogeneity while neglecting local temperature distribution inside cylinder during combustion process. In this study, a heat release analysis based on single-zone model has been evaluated by comparison with computational simulation result using Fire-code, which is based on multidimensional model (MDM). The limitations of the single-zone assumption have been estimated, To overcome these limitations, an improved model that includes the effects of spatial non-uniformity has been applied. From this improved single-zone heat release model (Improved-SZM), two effective values of specific heat ratios, denoted by ${\gamma}_V$ and ${\gamma}_H$ in this study, have been introduced. These values are formulated as the function of charge temperature changing rate and overall equivalence ratio. Also, it is applied that each equation of ${\gamma}_V$ and ${\gamma}_H$ has respectively different slopes according to several meaningful periods during combustion progress. The heat release analysis results based on improved single-zone model gives a good agreement with FIRE-code results over the whole range of operating conditions of target engine, Hyundai HiMSEN H21/32.