• 한국식물학회:학술대회논문집
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• 한국식물학회 1985년도 워크샵 및 심포지엄 북한산국립공원의 식생
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• pp.51-57
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• 1985

In order to evlauate feasibility of the gene tagging by the maize transposable element Ac in heterologous plant systems, we have investigated physical distances and directions of transposition of the element in Arabidopsis thaliana and tobacco cultured cell line BY-2. We prepared a T-DNA construct that carried a non-autonomous derivative of Ac with a site for cleavage by endonuclease I-Scel (designated dAc-I-RS element). Another cleavage site was also introduced into the T-DNA region outside dAc-I-RS. A number of transgenic Arabidopsis plants were generated, each of which had a single copy of the T-DNA at a different chromosomal location. To examine the pattern of transposition, three out of these transgenic plants were crossed with the Arabidopsis plant that carried the gene for Ac transposase and progeny in which dAc-I-RS had been transposed were isolated. After digestion of the genomic DNA of these progeny with I-SceI, sizes of segment of DNA were determined byd pulse-field gel electrophoresis. We also performed linkage analysis for the transposed elements and sites of mutations near the elements. Our results with three transgenic lines showed that 50% of all transposition events had occurred within 1, 700 kilo-base pairs (kb) on the same chromosome, with 35% within 200 kb, and that the elements transposed in both directions on the chromosome with roughly equal probability. The data thus indicate that the Ac-Ds system is most useful for tagging of genes that are present within 200 kb of the chromosomal site of Ac in Arabidopsis. In addition, determination of the precise localization of the transposed dAc-I-RS element should definitely assist in map-based cloning of genes around insertion sites. In the present paper, we report typical examples of such gene isolation studies.

• Journal of Welding and Joining
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• 제35권2호
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• pp.58-62
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• 2017

A319 aluminum alloy containing 6.5% Si and 3.5% Cu as major alloying elements has been widely used in machinery parts because of its excellent castability and crack resistance. However it needs more wear resistance to extend its usage to the severe wear environments. It has been known that hyper-eutectic Al-Si alloy having more than 12.6% Si contains pro-eutectic Si particles, which give better wear resistance and lubrication characteristics than hypo-eutectic Al-Si alloy like A319 alloy. In this study, it was tried to clad hyper-eutectic Al-Si alloy on the surface of A319 alloy. In the experiments, Al-36%Si alloy powder was mixed with organic binder to make a fluidic paste. The paste was screen-printed on the A319 alloy surface, melted by pulsed Nd:YAG laser and alloyed with the A319 base alloy. As experimental parameters, the average laser power was changed to 111 W, 202 W and 280 W. With increasing the average laser power, the melting depth was changed to $142{\mu}m$, $205{\mu}m$ and $245{\mu}m$, and the dilution rate to 67.2 %, 72.4 % and 75.7 %, and the Si content in the cladding layer to 16.2 %, 14.6 % and 13.7 %, respectively. The cross-section of the cladding layer showed very fine eutectic microstructure even though it was hyper-eutectic Al-Si alloy. This seems to be due to the rapid solidification of the melted spot by single laser pulse. The average hardness for the three cladding layers was HV175, which was much higher than HV96 of A319 base alloy. From the block-on-roll wear tests, A319 alloy had a wear loss of 5.8 mg, but the three cladding layers had an average wear loss of 3.5 mg, which meant that an increase of 40 % in wear resistance was obtained by laser cladding.

• The Korean Journal of Physiology
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• 제17권1호
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• pp.1-11
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• 1983

1) 토끼동방결절의 작은 절편에 미세 전극 두개로 voltage clamp를 하고 과분극에 의하여 활성화되는 내향전류, $i_f$의 동력학적 성상을 분석하였다. 2) 전류 $i_f$는 $10^{-7}g/ml$ TTX와 2 mM $Mn^{2+}$의 존재하에서 과분극 pulse에 의하여 활성화되었으며 그 범위는 $-45\;mV{\sim}-75\;mV$였다. 전류의 크기와 시간경과는 막전압이 과분극될수록 커지고 빨라졌다. 3) Envelope test결과 $i_f$전류는 단일 gate에 의하여 지수합수적 (exponential)으로 조절됨을 보였다. 4) 2 mM의 $Ba^{2+}$에 의하여$i_f$전류의 크기는 감소하고 시간경과도 느려졌으며 반응속도상수와 gating molecule의 열리고 닫히는 반응계수(rate coefficient; ${\alpha}_s$, ${\beta}_s$)와 막전압 관계곡선을 과분극쪽으로 이동시켰다. 이러한 $Ba^{2+}$의 효과는 24 mM $K^+$에 의하여 일부 상쇄되었다.

• 한국인터넷방송통신학회논문지
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• 제20권5호
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• pp.63-70
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• 2020

본 논문에서는 35 GHz 대역의 군 탐색기용 송수신 모듈을 설계 및 제작하였다. 밀리미터파 대역의 기판 성능과 집적도를 높이기 위해, 4층 액정 폴리머 기판을 개발하였다. 4층 액정 폴리머 기판은 3장의 FCCL 기판과 2장의 접착층으로 구현되었으며, 적층을 위해 기판간의 녹는 점 차이를 이용한 공정을 이용하였다. 스트립선로와 마이크로스트립 선로를 이용하여 기판의 길이에 따른 전송손실을 확인하였고, 35 GHz 대역의 전력분배기를 통해 액정폴리머 기판의 성능을 검증하였다. 이러한 기판을 이용하여 전력증폭기와 저잡음증폭기와 같은 송수신모듈을 구성하는 개별 블록에 대한 성능을 확인한 후, 단일 채널 Ka대역 송수신모듈을 4층 액정 폴리머 기판을 이용하여 개발하였다. 제작한 송수신모듈의 송신출력은 펄스 Duty 10%에서 1.1W 이상, 수신 잡음지수는 8.5 dB 이하, 수신 이득은 17.6 dB 이상의 수신 특성을 갖는다.

• Journal of Radiation Protection and Research
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• 제40권1호
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• pp.25-35
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• 2015

We analyzed the differential effects of histopathology, apoptosis and expression of radiation response genes after chronic low dose rate (LDR) and acute high dose rate (HDR) radiation exposure in spleen, lung and liver of rats. Female 6-week-old Sprague-Dawley rats were used. For chronic low-dose whole body irradiation, rats were maintained for 14 days in a $^{60}Co$ gamma ray irradiated room and received a cumulative dose of 2 Gy or 5 Gy. Rats in the acute whole body exposure group were exposed to an equal dose of radiation delivered as a single pulse ($^{137}Cs$-gamma). At 24 hours after exposure, spleen, lung and liver tissues were extracted for histopathologic examination, western blotting and RT-PCR analysis. 1. The spleen showed the most dramatic differential response to acute and chronic exposure, with the induction of substantial tissue damage by HDR but not by LDR radiation. Effects of LDR radiation on the lung were only apparent at the higher dose (5 Gy), but not at lower dose (2 Gy). In the liver, HDR and LDR exposure induced a similar damage response at both doses. RT-PCR analysis identified cyclin G1 as a LDR-responsive gene in the spleen of rats exposed to 2 Gy and 5 Gy gamma radiation and in the lung of animals irradiated with 5 Gy. 2. The effects of LDR radiation differed among lung, liver, and spleen tissues. The spleen showed the greatest differential effect between HDR and LDR. The response to LDR radiation may involve expression of cyclin G1.

• Structural Engineering and Mechanics
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• 제24권3호
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• pp.375-393
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• 2006

Under high velocity, pulse type near source earthquakes semi-active control systems are very effective in reducing seismic response base isolated structures. Semi-active control systems can be classified as: 1) independently variable stiffness, 2) independently variable damping, and 3) combined variable stiffness and damping systems. Several researchers have studied the effectiveness of independently varying damping systems for seismic response reduction of base isolated structures. In this study effectiveness of a combined system consisting of a semi-active independently variable stiffness (SAIVS) device and a magnetorheological (MR) damper in reducing seismic response of base isolated structures is analytically investigated. The SAIVS device can vary the stiffness, and hence the period, of the isolation system; whereas, the MR damper enhances the energy dissipation characteristics of the isolation system. Two separate control algorithms, i.e., a nonlinear tangential stiffness moving average control algorithm for smooth switching of the SAIVS device and a Lyapunov based control algorithm for damping variation of MR damper, are developed. Single and multi degree of freedom systems consisting of sliding base isolation system and both the SAIVS device and MR damper are considered. Results are presented in the form of nonlinear response spectra, and effectiveness of combined variable stiffness and variable damping system in reducing seismic response of sliding base isolated structures is evaluated. It is shown that the combined variable stiffness and variable damping system leads to significant response reduction over cases with variable stiffness or variable damping systems acting independently, over a broad period range.

• 대한전자공학회논문지SD
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• 제45권3호
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• pp.44-53
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• 2008

본 논문에서는 휴대용 오디고 제품의 헤드폰 구동을 위한 델타-시그마 변조기법 기반의 D급 증폭기를 제안한다. 제안된 D급 증폭기는 고성능 단일 비트 4차 델타-시그마 변조기를 이용하여 펄스폭 변조 신호를 발생시킨다. 높은 신호 대 잡음비를 얻는 것과 동시에 시스템의 안정성 확보를 위하여 시뮬레이션을 통해 변조기 루프필터의 폴과 제로를 최적화하였다. 테스트 칩은 $0.18{\mu}m$ CMOS 공정으로 제작되었다. 칩 면적은 $1.6mm^2$ 이며, 20Hz 부터 20kHz까지의 신호대역을 대상으로 동작한다. 3V 전원전압과 32옴의 로드를 사용하여 측정된 출력은 0.03% 이하의 전고조파 왜율을 갖는다.

• Annals of Clinical Neurophysiology
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• 제8권2호
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• pp.158-162
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• 2006

Background: It has been proposed that proprioceptive input can modulate neural excitability in both primary motor cortices (M1) simultaneously, although direct evidence for this is still lacking. Previous studies showed that proprioceptive accuracy of one hand is reduced after the application of one-Hz repetitive transcranial magnetic stimulation (rTMS) for 15 minutes over the contralateral somatosensory cortex. The aim of this study was to investigate the effect of rTMS-induced central proprioceptive deafferentation to excitability of both M1 as reflected in ipsilateral and contralateral motor evoked potentials (MEP). Methods: MEPs of both abductor pollicis bravis (APB) muscles were recorded using single-pulse TMS over right M1 in seven healthy subjects. Immediately after one-Hz rTMS was applied for 15 minutes over the right somatosensory cortex, the MEP measurement was repeated. The proprioceptive function of the left thumb was assessed, before and after rTMS, using a position-matching task. Results: There was an increase in ipsilateral MEP after the rTMS: whereas no MEPs were recorded on the ipsilateral hand before the rTMS, MEPs were recorded in both ipsilateral and contralateral hand in three of seven subjects. At the same time, the mean log amplitude was reduced and the mean latency was prolonged in the contralateral MEP. Conclusions: rTMS-induced central proprioceptive deafferentation reduces the MEP generation in the contralateral hand, and fascilitates that in the ipsilateral hand. A further study with a larger sample seems warranted to confirm this finding and to elucidate the neurophysiology underlying it.

• 대한화학회지
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• 제32권3호
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• pp.186-194
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• 1988

계면활성 micelle을 형성하는 acetonitrile 용액을 O,O-dimethyl-O-(3-methyl-4-nitrophenyl)-phosphorothioate (Fenitrothion)의 전기화학적 환원을 direct current 및 differential pulase polarography, cyclic voltammetry 그리고 controlled potential coulometry 방법으로 연구하였다. Fenitrothion의 환원과정은 1단계로 4 전자 이동에 의한 부분 가역적 전자이동 화학반응(EC, EC)기구로 O,O-dimethyl-O-(3-methyl-4-hydroxyaminophenyl)-phosphorothioate를 형성하고, 더 높은 음전위에서 2 전자 이동에 따른 양성자 반응으로 phosphorus 원자와 phenoxy group의 단일 결합이 끊어지면서 주 생성물인 p-amino-m-cresol과 dimethyl thiophosphinic acid를 생성하였다. Sodium lauryl sulfate micelle 용액에서 polarography 환원파는 전체적으로 억제 되었으며 특히 1차 환원파는 음이온 micelle의 선택적 작용으로 2단계로 분리되었다.

• 한국광학회지
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• 제11권1호
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• pp.37-42
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• 2000

반도체 광 증폭기의 특성을 이해하기 위하여 비율 방정식을 수치 해석적으로 풀었다. 사용된 비율 방정식은 운반자 밀도와 자발 방출된 빛, 그리고 신호 빛을 위치와 시간의 함수로 기술할 수 있다. 사각 파형의 신호가 입사했을 경우에 대하여 위의 세가지 양을 구하여서 반도체 광증포기의 동적인 특성을 파악하였다. 파장 다중 광통신에서처럼 다중신호가 입사하는 경우의 비선형성을 분석하였다. 맥놀이에 의한 내부변조 변형을 계산하여 본 결과 파장 간격이 넓을수록 내부변조 변형은 줄어드는 결과를 정량적으로 얻었으며, 신호간의 채널 간섭은 신호의 세기가 감소하면 줄어드는 결과를 얻었다. 또한 신호로 사용되는 파장에서 충분히 멀리 떨어진 연속파동 레이저가 입사하는 경우에는 채널간의 신호간섭과 출력파형 변형이 감소하는 이득고정 효과가 나타남을 계산을 통하여 확인하였다.