• Bulletin of the Korean Chemical Society
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• 제23권8호
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• pp.1139-1143
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• 2002

Analysis of lysophosphatidic acids (LPAs) is of clinical importance as they can serve a potential marker for ovarian and other gynecological cancers and obesity. It is critically important to develop a highly sensitive and specific method for the early detection of gynecological cancers to improve the overall outcome of this disease. We have established a novel quantification method of LPAs in human plasma by negative ionization tandem mass spectrometry (MS-MS) using multiple reaction monitoring (MRM) mode without the conventional TLC step. Protein-bound lipids, LPAs in plasma were extracted with methanol : chloroform (2:1) containing LPA C14:0 as an internal standard under acidic condition. Following back extraction with chloroform and water, the centrifuged lower phase was evaporated and reconstituted in methanol. The reconstituted solution was directly injected into electrospray source of MS/MS. For MRM mode, Q1 ions selected were m/z 409, 433, 435, 437 and 457 which corresponds to molecular mass [M-H]- of C16:0, C18:2, C18:1, C18:0 and C20:4 LPA, respectively. Q2 ions selected for MRM were m/z 79, phosphoryl product. Using MS/MS with MRM mode, all the species of LPAs were completely separated from plasma matrix without severe interferences. This method allowed simultaneous detection and quantification of different species of LPAs in a plasma over a linear dynamic range of 0.01-25 ㎛olL-1 . The detection limit of the method was 0.3 pmol/mL, with a correlation coefficient of 0.9983 in most LPAs analyzed. When applied to the plasmas of normal and gynecological cancer patients, this new method differentiated two different groups by way of total LPA level.

• Wind and Structures
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• 제17권1호
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• pp.43-68
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• 2013

The fluctuating wind induced vibration is one of the most important factors which has been taken into account in the design of long-span bridge due to the low stiffness and low natural frequency. Field measurement characteristics of sustained wind on structure site can provide accurate wind load parameters for wind field simulation and structural wind resistance design. As a suspension bridge with 1490 m main span, the Runyang Suspension Bridge (RSB) has high sensitivity to fluctuating wind. The simultaneous and continuously wind environment field measurement both in mid-span and on tower top is executed from 2005 up to now by the structural health monitoring system installed on this bridge. Based on the recorded data, the wind characteristic parameters, including mean wind speed, wind direction, the turbulence intensity, the gust factors, the turbulence integral length, power spectrum and spatial correlation, are analyzed in detail and the coherence functions of those parameters are evaluated using statistical method in this paper. The results indicate that, the turbulence component of sustain wind is larger than extremely strong winds although its mean wind speed is smaller; the correlation between turbulence parameters is obvious; the power spectrum is special and not accord with the Simiu spectrum and von Karman spectrum. Results obtained in this study can be used to evaluate the long term reliability of the Runyang Suspension Bridge and provide reference values for wind resistant design of other structures in this region.

• 한국철도학회논문집
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• 제19권4호
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• pp.445-455
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• 2016

실시간 철도안전 관제시스템은 철도시스템을 구성하는 열차, 신호, 전력 및 설비 등으로 분산되어 감시되던 시스템의 안전관련 데이터를 통합하여 안전감시 효율을 향상시키고 사고를 예방하는 것이 목적으로, 기존 개별 감시 시스템과 달리 데이터의 대용량 처리와 실시간 처리 성능을 동시에 요구하고 있다. 기존 관제시스템에서 주로 활용되는 디스크 기반 데이터베이스는 실시간 및 빅 데이터 처리기능이 없고, 최근 도입되는 메모리 기반 데이터베이스는 빅데이터 처리기능이 없으며, 시계열 데이터베이스는 실시간 처리 기능이 없다. 이에 따라, 실시간 안전관제에서 요구되는 빅 데이터 처리와 실시간 처리를 동시에 제공하는 새로운 솔루션이 필요하다. 본 연구에서는 기존 관제의 데이터 처리 사례를 분석하고, 빅 데이터 처리와 실시간 처리를 동시에 제공하는 새로운 데이터 처리 방안을 제안하였으며, 이를 검증하였다.

• 한국공간구조학회논문집
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• 제19권1호
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• pp.117-126
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• 2019

Micro-Electro-Mechanical Systems (MEMS) sensors have been widely used in Structural Health Monitoring due to their convenience and lower costs in comparison to conventional sensors. Triggered measurements are relevant in events such as earthquakes because unlike continuous measurements, they only record the structural response once an event happens. This is more cost effective and it makes the data more manageable because only the required measurements from the event are recorded. The most common method of triggering is amplitude triggering. However, lower input amplitudes (less than 0.1g) cannot be triggered by using this method. In this paper, sound triggering was introduced to allow triggered measurements for lower input amplitude values. The performance of the sound triggering and amplitude triggering were compared by a series of shaking-table tests. It was seen that sound-triggering method has a wider frequency (0.5~10Hz) and amplitude (0.01~1.0g) range of measurements. In addition, the sound triggering method performs better than the amplitude triggering method at lower amplitudes. The performance of the amplitude triggering, in terms of the triggering being simultaneous improves at higher input amplitudes.

• 융합정보논문지
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• 제11권2호
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• pp.107-116
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• 2021

본 연구에서는 동물식품 내 잔류하는 동물의약품 중 성장보조제인 에스트라디올(Estradiol-17��), 테스토스테론(Testosterone), 프로게스테론(Progesterone)에 대한 분석법을 개발하고자 하였다. 분석대상 물질은 액체크로마토그라피를 사용하여 분리하였고, electrospray ionization(ESI) 과정을 거쳐 질량분석기에 주입되어 multiple reaction monitoring(MRM) 모드로 검출하였다. 개발된 분석법은 CODEX CAC/GL 71-2009에 근거하여 유효성을 검증하였고, 분석의 실효성 허용범위를 충족함을 확인하였다. 국내 유통되는 소고기, 돼지고기, 닭고기에 대해 확립된 분석법으로 분석을 진행하여 실제시료 적용성을 확인하였다. 이를 통해 개발된 분석법이 국내 유통 축산물에서 확인되고 있는 성장보조제 일부에 대해 신속하고 신뢰성 높은 분석이 가능함을 확인하였다. 후속 연구를 통해 확립된 분석법을 기반으로 분석대상 성장보조제의 범위를 확장하고, 이를 모두 포함시켜 동시분석법을 확립한다면 활용성이 높은 분석법이 완성될 것으로 확신한다.

• 한국ITS학회 논문지
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• 제22권1호
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• pp.115-125
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• 2023

본 논문은 교통상태 감시 등 교통관제를 위해 설정한 CCTV 카메라의 화각 상태에서 교통량, 속도 등 교통정보 수집을 동시에 수행할 수 있는 CCTV 카메라 자세보정 알고리즘 개발에 관한 것이다. 개발한 자율자세보정 알고리즘은 차량인식 및 추적기법을 이용하여 도로를 식별하고, 운영자의 교통감시 및 교통정보 수집을 위한 화각을 결정한다. 제안 알고리즘의 성능검증은 현장에 설치한 CCTV를 이용하였으며, 교통감시 및 교통정보 수집을 위해 각각 설정한 화각에 대해 자율자세보정 알고리즘이 자동 산출한 화각의 결과와 비교하였다. 분석결과 운영자 감시를 위한 화각은 상호 96%의 일치성을 보였다. 교통정보의 경우는 교통량 및 속도의 정확도가 각각 96%, 95%로 산출됐으며 수동 설정한 화각과 비교할 때 약 2%의 오차가 발생하는 것으로 나타났다. 결과적으로 제안 알고리즘을 통해 관제용 CCTV를 이용하여 교통정보 수집 및 교통상황 감시를 동시에 수행할 수 있음을 확인하였다.

• 한국환경농학회지
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• 제36권4호
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• pp.279-287
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• 2017

본 연구에서는 국내 320종 다성분 동시분석법에 적용되지 있지 않으면서 실제 수출딸기의 농가에서 많이 사용하고 있는 cyenopyrafen과 cyflumetofen의 잔류농약 소실특성과 실제 수출딸기의 잔류농도를 확인하기 위해 수행하였다. 각 농약은 살포 후, 5일과 1일 이후에 국내 MRL 기준이하로 검출되었지만 cyenopyrafen는 30일, cyflumetofen는 45일 이후부터 PLS 기준 이하로 검출되는 것으로 확인하였다. 따라서, 해당농약의 잔류허용기준이 설정되어있지 않은 국가에 수출할 경우에는 살포 후 30일 또는 45일 경과 후에 수확하는 것이 바람직한 할 것으로 보인다. 또한 2016년과 2017년에 총 40농가에의 수출딸기를 무작위로 채취하여 분석한 결과, cyenopyrafen은 21농가, cyflumetofen은 10농가에서 주요 수출대상국의 PLS 기준을 초과하는 것으로 확인되었다. 수출딸기의 재배에 많이 사용되는 52종의 농약성분을 2016년과 2017년에 총 40농가에서 시료를 채취하여 동시다 성분 분석으로 모니터링 한 결과, 12종의 농약성분이 국내주요수출 국가인 홍콩과 싱가포르의 잔류허용기준에 부적합한 것으로 확인하였다. 그러므로, 추후 수출딸기의 잔류농약에 대한 안전성 및 신뢰성문제가 야기될 소지가 있기 때문에 각 농가와 각 부처의 각별한 주의와 지속적인 지원 및 모니터링이 필요할 것으로 사료된다.

• Bulletin of the Korean Chemical Society
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• 제31권5호
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• pp.1192-1198
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• 2010

Pharmaceuticals and personal care products (PPCPs) are emerging contaminants in the aquatic environment. Many pharmaceuticals are not completely removed during wastewater treatment, leading to their presence in wastewater treatment effluents, rivers, lakes, and ground water. Here, we developed analytical methods for monitoring ten pharmaceuticals from surface water by LC/ESI-MS/MS. For sample clean-up and extraction, MCX (mixed cation exchange) and HLB (hydrophilic-lipophilic balance) solid-phase extraction (SPE) cartridges were used. The limits of detection (LOD) in distilled water and the blank surface water were in the range of 0.006 - 0.65 and 1.66 - 45.05 pg/mL, respectively. The limits of quantitation (LOQ) for the distilled water and the blank surface water were in the range of 0.02 - 2.17 and 5.52 - 150.15 pg/mL, respectively. The absolute recoveries for fortified water samples were between 62.1% and 125.4%. Intra-day precision and accuracy for the blank surface water were 2.9% - 24.1% (R.S.D.) and -16.3% - 16.3% (bias), respectively. In surface wastewater near rivers, chlortetracycline and acetylsalicylic acid were detected frequently in the range of 0.017 - 5.404 and 0.029 - 0.269 ng/mL, respectively. Surface water near rivers had higher levels than surface water of domestic treatment plants.

• Advances in materials Research
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• 제1권2호
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• pp.129-146
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• 2012

Polycrystalline diamond is an ideal material for parts with micro-holes and has been widely used as dies and cutting tools in automotive, aerospace and woodworking industries due to its superior wear and corrosion resistance. In this research paper, the modeling and simultaneous optimization of multiple performance characteristics such as material removal rate and surface roughness of polycrystalline diamond (PCD) with ultrasonic machining process has been presented. The fuzzy logic and taguchi's quality loss function has been used. In recent years, fuzzy logic has been used in manufacturing engineering for modeling and monitoring. Also the effect of controllable machining parameters like type of abrasive slurry, their size and concentration, nature of tool material and the power rating of the machine has been determined by applying the single objective and multi-objective optimization techniques. The analysis of results has been done using the MATLAB 7.5 software and results obtained are validated by conducting the confirmation experiments. The results show the considerable improvement in S/N ratio as compared to initial cutting conditions. The surface roughness of machined surface has been measured by using the Perthometer (M4Pi, Mahr Germany).

• Bulletin of the Korean Chemical Society
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• 제31권7호
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• pp.1897-1901
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• 2010

Multi-wall carbon nanotube (MWNT)-modified glassy carbon electrodes (GCE) were prepared for simultaneous determination of rutin and quercetin. Microbial carbohydrates, $\alpha$-cyclosophorohexadecaose ($\alpha$-C16) and succinoglycan monomer M3 (M3) were doped into MWNTs to prepare a $\alpha$-C16-doped MWNT-modified GCE (($\alpha$-C16 + MWNTs)/GCE) and a M3-doped MWNT-modified GCE ((M3 + MWNTs)/GCE), respectively. The sensitivities of the ($\alpha$-C16 + MWNTs)/GCE to rutin and quercetin were 34.7 ${\mu}A\cdot{\mu}M^{-1}{\cdot}cm^{-2}$ and 18.3 ${\mu}A\cdot{\mu}M^{-1}{\cdot}cm^{-2}$, respectively, in a linear range of $2\sim8{\mu}M$ at pH 7.2. The sensitivities of the (M3 + MWNTs)/GCE was 2.44 ${\mu}A\cdot{\mu}M^{-1}{\cdot}cm^{-2}$ for rutin and 7.19 ${\mu}A\cdot{\mu}M^{-1}{\cdot}cm^{-2}$ for quercetin without interference.