• 한국양식학회지
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• 제8권4호
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• pp.355-366
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• 1995

해산패류양식에서 먹이 생물로서 이용 가능한 7종 규조류(Chaetoceros simplex, Navicula incerta, Phaeodactyiurn tricornutum, Nitzschia closterium, Skeletonema costatum, Thalassiosira pseudonana, Thalassiosira weisspogii)의 먹이 효율을 알아보기 위하여 일간 성장율, 평균 세포 용적, 화학적 성분과 일반 지방산 성분에 관하여 실험하였다. 세포용적을 보면 T pseudonana가 $(125{\mu}m^3)$로서 가장 작았으며 T. weissfogii가 $(824{\mu}m^3)$로서 가장 크게 나타났다. 그러나, 7일간 배양한 7종의 성장율을 살펴보면 S. costatum이 s.g.r.가 0.9928로 가장 높게 나타났고, 세포 용적이 가장 큰 T. weissflogii가 0.5016로서 가장 낮게 나타났다. 조단백질 함량은 T. weissflogii가 $24.4\%$로 가장 높게 나타났고, T. pseudonana, S. costatum이 각각 $19.0\%,\;19.8\%$로 비교적 낮게 나타났다. 조지방 성분 함량은 C. simplex가 $8.4\%$로 가장 높게 나타났으며 다음으로는 N closterium, M incerta, T. weissflogii의 순서였다. 7종 규조류의 주요 지방산 성분은 14:0, 16:0, 16:1 및 18:1이었으며 고도불포화 지방산함량은 비교적 낮게 나타났다. 20:5n-3 (EPA)는 P. tricornutum, C. simplex에 서 각각 $7.73\%,\;5.87\%$의 비율이었으며, 22:6 n-3 (DHA)는 P. tricornutum $0.84\%$, C. simplex $1.75\%$, S. costatum $0.70\%$의 함량을 나타내었다. 참굴 유생의 D상과 초기 각정기 유생에 7종의 규조류를 공급한 먹이효율 결과를 보면, C. simplex 공급구가 성장 및 생존율이 높게 나타났고, S. costatum과 T. weissflogii 공급구가 저조하였다. 이러한 결과를 볼때 C. simplex가 실험에 이용되었던 다른 규조류보다 이매패류의 유생에 더 유용한 먹이생물이 라고 사료된다.

• Parasites, Hosts and Diseases
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• 제50권2호
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• pp.165-171
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• 2012

Larval excretory-secretory products of Anisakis simplex are known to cause allergic reactions in humans. A cDNA library of A. simplex 3rd-stage larvae (L3) was immunoscreened with polyclonal rabbit serum raised against A. simplex L3 excretory-secretory products to identify an antigen that elicits the immune response. One cDNA clone, designated as ${\alpha}$-methylacyl CoA racemase (Amacr) contained a 1,412 bp cDNA transcript with a single open reading frame that encoded 418 amino acids. A. simplex Amacr showed a high degree of homology compared to Amacr orthologs from other species. Amacr mRNA was highly and constitutively expressed regardless of temperature (10-$40^{\circ}C$) and time (24-48 hr). Immunohistochemical analysis revealed that Amacr was expressed mainly in the ventriculus of A. simplex larvae. The Amacr protein produced in large quantities from the ventriculus is probably responsible for many functions in the development and growth of A. simplex larvae.

• Parasites, Hosts and Diseases
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• 제49권1호
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• pp.39-44
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• 2011

The infection status of marine fish and cephalopods with Anisakis simplex third stage larva (L3) was studied over a period of 1 year. A total of 2,537 specimens, which consisted of 40 species of fish and 3 species of cephalopods, were purchased from the Cooperative Fish Market in Busan, Korea, from August 2006 to July 2007. They were examined for A. simplex L3 from the whole body cavity, viscera, and muscles. A. simplex L3 were confirmed by light microscopy. The overall infection rate reached 34.3%, and average 17.1 larvae were parasitized per infected fish. Fish that recorded the highest infection rate was Lophiomus setigerus (100%), followed by Liparis tessellates (90%), Pleurogrammus azonus (90%), and Scomber japonicus (88.7%). The intensity of infection was the highest in Gadus macrocephalus (117.7 larvae per fish), followed by S. japonicus (103.9 larvae) and L. setigerus (54.2 larvae). Although abundance of A. simplex L3 was not seasonal in most of the fish species, 10 of the 16 selected species showed the highest abundance in February and April. A positive correlation between the intensity of L3 infection and the fish length was obvious in S. japonicus and G. macrocephalus. It was likely that A. simplex L3 are more frequently infected during the spring season in some species of fish. Our study revealed that eating raw or undercooked fish or cephalopods could still be a source of human infection with A. simplex L3 in Korea.

• 한국해양바이오학회지
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• 제12권2호
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• pp.75-80
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• 2020

In the present study, the estrogenic activity and anti-osteoclastogenic activity of the Myelophycus simplex extract were evaluated using T47D-Kbluc cells and bone marrow-derived macrophages (BMMs). As a result of the measurement of the estrogenic activity in the T47D-Kbluc cell line, the Myelophycus simplex extract showed increased estrogenic activity in a dose-dependent manner in association with its concentration. To confirm the regulatory effect of the Myelophycus simplex extract on the estrogen-responsive gene, the Myelophycus simplex extract showed a similar tendency to estradiol: the expression of estrogen receptor 1 (ESR1) was significantly decreased while the expression of estrogen receptor 2 (ESR2) was increased. Furthermore, the Myelophycus simplex extract exhibited an inhibitory effect on osteoclast differentiation. In conclusion, these Myelophycus simplex extracts might be regarded as candidates for further studies or the development of functional food products or medicine to prevent or avoid postmenopausal symptoms for women.

• 경영과학
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• 제11권3호
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• pp.13-26
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• 1994

In this paper we studied an integration scheme of some simplex algorithms and some efficient techniques to get the stable solution in linear programming code. And we developed a linear programming package (LPAK) by introducing this scheme and techniques. In LPAK three different algorithms were integrated, which were two primal simplex algorithms using Two phase method and big-M method respectively, and the dual simplex algorithm. LPAK introduces several heuristic techniques in each step of simplex method in order to enhance the stability and efficiency. They were new heuristic methods in structuring initial basis, choosing entering variable, choosing dropping variable and performing reinversion. The experimental results on the NETLIB problems showed that LPAK provided the stable solutions.

• Nonlinear Functional Analysis and Applications
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• 제26권4호
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• pp.701-716
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• 2021

In this present article, we construct a new framework that's we call the weighted geometric realization of 2 and 3-simplexes. On this new weighted framework, we construct a nonself-adjoint 2-simplex Laplacian L and a self-adjoint 2-simplex Laplacian N. We propose general conditions to ensure sectoriality for our new nonself-adjoint 2-simplex Laplacian L. We show the relation between the essential spectra of L and N. Finally, we prove the absence of the essential spectrum for our 2-simplex Laplacians L and N.

• 한국식품위생안전성학회지
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• 제23권4호
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• pp.314-318
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• 2008

This research aimed to compare the detection methods of Anisakis simplex in Sea fish by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and macroscopic inspection. We examined 18 Trichiurus lepturus, 11 Scomber japonicus, and 65 Todarodes pacificus collected from the retail markets in the areas of Uljin, Kyuonggi province and Seoul. As the result of examinations, we found that detection rate of Anisakis simplex by macroscopic observation was 89% in Trichiurus lepturus, 90.9% in Scomber japonicus, 32.3% in Todarodes pacificus. The detection rate of Anisakis simplex by PCR-RFLP was 77.7% in Trichiurus lepturus, 81.8% in Scomber japonicus, 26.1% in Todarodes pacificus. We could conclude that PCR-RFLP method of Anisakis simplex was more specific rather than macroscopic observation.

• 한국산업정보학회논문지
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• 제6권1호
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• pp.15-23
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• 2001

본 논문에서는 동기 BPSK 직접확산 부호분할 다원접속(DS/CDMA)시스템에서 발생하는 다원접속 간섭을 제거할 수 있는 시스템을 제시한다. 확산부호로는 오프셋된 PN 부호와 Simplex 부호를 사용하며 임의의 순간에서는 PN 부호 또는 Simplex 부호 중 하나만을 사용하는 것으로 한다. 수신기는 기준 상관수신기를 이용하여 정보를 검출하고 다원접속간섭을 제거하도록 구성한다. 제안된 시스템을 수식적으로 해석하며 이러한 해석 결과로 제안된 시스템은 다원접속간섭을 상당히 제거함을 알 수 있다. 또한 AWGN 환경 및 나카가미 페이딩 하에서 임의의 사용자에서 발생하는 간섭을 해석하고 평균 오류확률을 분석한다.

• Archives of Plastic Surgery
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• 제37권5호
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• pp.687-690
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• 2010

Purpose: Hidroacanthoma simplex (HAS) is a rare benign neoplasm of the skin and known as intraepidermal poroma. Malignant transformation of HAS has been reported in the literature, but it is very rare. We experienced a case of malignant hidroacanthoma simplex (MHA) and performed the surgery of the neoplasm. Methods: A 73-year-old patient visited our department for evaluation and treatment of the skin lesion ($3.2{\times}3.0\;cm^2$) that occurred two years before on the right ankle area. The lesion was characterized by dark-brown, hyperkeratotic and protruding patch. The patient had no symptom, and any palpable lymph node was not found on physical examination. The result of laboratory was also nonspecific. The lesion was excised completely with a resection margin of 5 mm including subcutaneous tissue partially, followed by full-thickness skin graft for reconstruction. Results: The result of biopsy was reported as malignant hidroacanthoma simplex. Histologically, the epidermis showed discrete aggregates of tumor cells which had round, hyperchromatic nuclei and pale or clear abundant cytoplasm. Cystic spaces were found within the tumor and mitosis were seen, frequently. No recurrence was found one month after surgery. Conclusion: The case of malignant hidroacanthoma simplex was treated with full-thickness skin graft following wide excision of the tumor.

• 한국수자원학회논문집
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• 제35권5호
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• pp.541-552
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• 2002

본 연구에서는 전체탐색기법 중 Simplex법의 원리를 이용한 SCE-UA법과 Annealing-Simplex (A-S)법을 일유출량 추정 수문모형인 탱크모형의 매개변수 보정에 적용하여 국부탐색기법인 Downhill Simplex법의 결과와 비교하여 탐색능력을 평가하였다. 오차가 없는 합성자료를 사용한 보정에서 A-S법이 목적함수에 관계없이 전역최적해를 탐색하는 결과를 나타냈으며, SCE-UA법은 ABSERR를 목적함수로 사용할 경우에 전역최적해를 탐색하는 결과를 나타냈으며, 다른 목적함수를 사용하는 경우에는 최적해에 가까운 탐색결과를 나타냈다. Downhill Simplex법은 초기값에 따라 다근 탐색결과를 나타냈으며, 최적해에 가까운 초기값을 사용할 경우 전역 최적해를 탐색하는 결과를 나타냈다. 실측자료를 사용한 보정에서는 A-S법과 SCE-UA법이 목적함수에 관계없이 양호한 결과를 나타냈다. 두 개의 서로 다른 단일 목적함수를 조합하여 만든 목적함수 중 DRMS와 NS의 조합에 의해 만들어진 목적함수인 DN이 다른 목적함수보다 저유량에 비중을 더 둔 예측결과를 나타냈으며, 전체 자료기간에 대해서 양호한 예측결과론 나타냈다.