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Analysis of Renal Adverse Reaction Caused by Amphotericin B (Amphotericin B 투여에 의한 신장 유해반응 분석)

  • Lee, Song Bin;Kim, Tae Kyung;Ko, Jong Hee;Ahn, Ji Hyune;Kim, Sung Eun;Seok, Hyun Ju;Kim, Hyunah
    • Korean Journal of Clinical Pharmacy
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    • v.22 no.4
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    • pp.340-346
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    • 2012
  • Background: Amphotericin B is a mainstay in the treatment of many systemic fungal infections due to its wide antifungal spectrum and low incidence of resistance. However, the use of amphotericin B is limited by its nephrotoxicity. Objectives: The objective of this study was to evaluate the incidence and risk factors of renal adverse drug reactions (ADRs) of conventional amphotericin B (Fungizone$^{(R)}$). In addition, we compared the changes of serum creatinine (SCr) between patients who remained conventional amphotericin B and patients who were switched to liposomal amphotericin B after occurrence of renal adverse reactions. Methods: Adult hospitalized patients who reported renal adverse reactions caused by conventional amphotericin B from January 2011 to July 2012 at pharmacovigilance center in Yonsei University Healthcare System included in this study. ADRs scored as 'doubtful' in Naranjo probability ADR scale were excluded. We retrospectively analyzed patients' basic clinical characteristics, concurrent diseases or nephrotoxic drugs in order to find variables that can correlate with occurrence of renal ADRs. Changes in SCr were compared between conventional amphotericin B group and liposomal amphotericin B group. Results: A total of 231 ADRs after administration of conventional amphotericin B in 75 patients were reported to pharmacovigilance center and assessed their severities as 'possible', 'probable', or 'definite'. Renal adverse reaction was the most common ADR with incidence rate of 42% (96 of 231 ADRs). Mean change in SCr from baseline was 0.26 mg/dL (change % 37.8) and statistically significant (p=0.000). Simple correlations analysis revealed that the number of concurrent diseases and number of nephrotoxic drugs were positively correlated with changes in SCr, but these results were not statistically significant. Among 43 patients who remained amphotericin B after occurrence of renal ADRs, 27 patients was administered conventional amphotericin B and 16 patients changed to liposomal amphotericin B. Mean change in SCr in amphotericin B group was 0.23 mg/dL (32.75%), whereas mean change in SCr in liposomal amphotericin B group were -0.28 mg/dL (19.38%) and difference between two groups was statistically significant (p=0.003). The numbers of patient with SCr elevation more than 30% were 9 (33.3%) in amphotericin B group and 2 (12.5%) in liposomal amphotericin B group (Odd Ratio=3.50, 95% Confidence Interval 0.65-18.85; p=0.130). Conclusion: An analysis of ADRs due to amphotericin B administration revealed significant mean changes in SCr from baseline. Switching to liposomal amphotericin B showed significant decrease in SCr compared with conventional amphotericin B.

A Comparative Study on the Herbage Utilization for Mixture Types by Korean Native Goats (한국재래산양에 의한 혼파유형별 목초의 이용성 비교 연구)

  • Lee, In-Duk;Lee, Hyung-Suk
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.25 no.3
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    • pp.185-190
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    • 2005
  • The objective of this experiment was to compare the dry matter intake, nutrients digestibility and nitrogen and energy utilization of herbages from mixtures types; conventional mixtures(orchardgrass 50+ tall fescue 20+perennial ryegrass 10+Kentucky bluegrass 10+white clover $10\%$), complex mixtures (orchardgrass 40+ tall fescue 20+perennial ryegrass 10+Kentucky bluegrass 10+redtop 10+alfalfa 5+red clover $5\%$) and simple mixtures(orchardgrass 80+red clover $20\%$) by Korean native goats. This experiment was conducted by total collection method in laboratory, 2000. The voluntary DM intake per body weight was slightly higher f3r complex mixtures (30.2g) than those of other mixtures. but there was no significant difference. The digestibility of dry matter and cellular constituents were slightly higher for complex mixtures than those of other mixtures (p<0.05), but NDF and ADF digestibilities did not differ among mixtures. The retained nitrogen percent (apparently biological value) was slightly higher fer complex mixtures $(55.1\%)$ than those of other mixtures, but there was no significant difference among mixtures. Apparently retained digestible energy was slightly higher for complex mixtures $(60.2\%)$ than those of other mixtures (p<0.05). Based on the results, the nitrogen and energy utilization of herbage by Korean native goats were slightly higher for complex mixtures than those of other mixtures.

Determination of 10${\alpha}$-Methoxy-9,10-dihydrolysergol (MDL), Main Metabolite of Nicergoline, in Human Plasma by HPLC-MS and Applicability to Oral Bioavailability in Korean Healthy Male Volunteers (HPLC-MS를 이용한 생체시료 중 니세르골린의 주대사체인 10${\alpha}$-Methoxy-9,10-dihydrolysergol(MDL)의 분석 및 이를 이용한 한국인 성인 남성에 대한 생체이용률 응용)

  • Lim, Hyon-Kyun;Yoo, Sun-Dong;Kim, Kyeong-Ho;Han, Sang-Beom;Youm, Jeong-Rok
    • YAKHAK HOEJI
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    • v.51 no.2
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    • pp.133-139
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    • 2007
  • A simple and sensitive HPLC-MS method for quantitation of 10${\alpha}$-methoxy-9,10-dihydrolysergol (MDL), the main metabolite of nicergoline, in human plasma was developed and the bioavailability parameters of MDL was assessed in Korean healthy male volunteers. Clomipramine was used as an internal standard. MDL and internal standard in plasma sample were extracted using ethyl acetate. A centrifuged upper layer was then evaporated and reconstituted with mobile phase of 10 mM ammonium acetate-acetonitrile (10 : 90, v/v). The reconstituted samples were injected into a Zorbax SB-C8 column (2.1${\times}$150 mm,5 ${\mu}$m) at a flow-rate of 0.3 ml/min. Using MS with selected ion monitoring (SIM) mode, MDL and clomipramine were detected without severe interference from human plasma matrix. MDL produced a protonated molecular ion ([M+H]$^+$) at m/z 287. Internal standard produced a protonated molecular ion ([M+H]$^+$) at m/z 315. A linear relationship for MDL was found in the range of 2.5${\sim}$100 ng/ml. The lower limit of quantitation (LLOQ) was 2.5 ng/ml with acceptable precision and accuracy. The intra- and inter-day validation for all coefficients of variation (R.S.D.%) were found less than 15%. Main pharmacokinetic parameters of 30 mg of nicergoline were revealed as follows: AUC$_t$ 321.1${\pm}$64.5 ng${\cdot}$hr/ml, C$_{max}$, 51.2${\pm}$25.3 ng/ml, T$_{max}$ 3.6${\pm}$1.5 hr, K$_{el}$ 0.12${\pm}$0.07 hr$^{-1}$ and t$_{1/2}$ 7.6${\pm}$3.4 hr. Inter subject variations and race differences were shown in comparison with the published data in the literature.

Framework for Car Safety Education Virtual Reality Simulation (자동차 안전교육 VR 시뮬레이션 제작을 위한 프레임워크)

  • Xie, Qiao;Ding, Xiu Hui;Jang, Young-Jick;Yun, Tae-Soo
    • Journal of the Korea Convergence Society
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    • v.10 no.9
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    • pp.37-45
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    • 2019
  • In recent years, the emergence of virtual reality (VR Virtual Reality) technology has provided a new model of safety education, enabling users to learn and respond to disasters in a virtual safety education environment. However, the related VR products related to domestic and foreign R & D are relatively simple, there is no practical training on specific accident, and it is not practical enough to play a sufficient role in safety education. In this paper, the problems and disadvantages of VR technology applied in the field of automobile safety education as an example of automobile accident among the types of disasters are examined, and a system framework of automotive safety education based on VR technology is proposed. The vehicle safety education system proposed in this paper will help users to improve driving safety consciousness, to acquire safety knowledge in driving, and to acquire driving safety skill which is very important for automobile safety education. In addition, the design and production methods of safety education based on VR technology are considered to have important reference implications for the application of modern teaching and teaching theory by integrating with VR technology and developing related teaching materials products and finally introducing education.

Development of a rapid HPLC method for the determination of penciclovir in human plasma using a monolithic column and its application to a bioequivalence study (모노리틱 칼럼을 이용한 혈장 중 펜시를로버의 HPLC 신속분석법 개발 및 이를 이용한 생물학적동등성시험)

  • Kim, Jin Hee;Park, Ah Yeon;Jung, Eun Ha;Lee, Cheol Woo;Lee, Tae Ho;Youm, Jeong-Rok
    • Analytical Science and Technology
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    • v.20 no.4
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    • pp.323-330
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    • 2007
  • A simple and rapid HPLC method with fluorescence detection(FLD) for quantitation of penciclovir in human plasma using a monolithic column was developed and validated. Penciclovir and ganciclovir(internal standard, I.S.) were separated on a Chromolith column RP-18e ($4.6{\times}100mm$) with a mobile phase consisting of a mixture of (A) methanol/50 mM sodium phosphate buffer containing 200 mg/L sodium dodecyl sulfate (3/97, pH 2.5) and (B) methanol/50 mM sodium phosphate buffer containing 200 mg/L sodium dodecyl sulfate (50/50, pH 2.5) at a flow gradient of $1.6{\sim}4.0mL/min$. The retention times of penciclovir and internal standard were less than 4.0 min. Calibration curve was linear ($R^2=0.9994$) over a concentration range of $0.1{\sim}5{\mu}g/mL$. Intra-day precision, accuracy and inter-day precision were 1.36~8.55 %, 92.8~100.0 % and 0.93~5.62 %, respectively with a limit of quantitation at $0.1{\mu}g/mL$. The present HPLC-FLD method is sensitive, precise and accurate. The method described herein has been successfully used for the bioequivalence study of a famciclovir formulation product after oral administration to healthy Korean volunteers.

Analysis of Cellular Fatty Acid Methyl Esters (FAMEs) for the Identification of Bacillus anthracis (균체 지방산 분석을 이용한 Bacillus anthracis의 동정)

  • Kim, Won-Yong;Song, Tae-Wook;Song, Mi-Ok;Nam, Ji-Yeon;Park, Chul-Min;Kim, Ki-Jung;Chung, Sang-In;Choi, Chul-Soon
    • The Journal of the Korean Society for Microbiology
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    • v.35 no.1
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    • pp.31-40
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    • 2000
  • Bacillus anthracis, the etiological agent of anthrax has been classified into the Bacillus subgroup I with B. cereus, B. mycoides and B. thuringiensis based on morphological and DNA similarity. DNA studies have further indicated that these species have very AT-rich genomes and high homology, indeed it has been proposed that these four sub-species be recognized as members of the one species. Several methods have been developed to obtain good differentiation between these species. However, none of these methods provides the means for an absolutely correct differntiation. The analysis of fatty acid methyl esters (FAMEs) was employed as a quick, simple and reliable method for the identification of 21 B. anthracis strains and closley related strains. The most significant differences were found between B. anthracis and B. anthracis closely related strains in FAMEs profiles. All tested strains of B. anthracis had a branched fatty acid C17:1 Anteiso A, whereas the fraction of unsaturated fatty acid Iso C17:1 w10c was found in B. anthracis closely related strains. By UPGMA clustering analysis of FAMEs profiles, all of the tested strains were classified into two clusters defined at Euclidian distance value of 24.5. The tested strains of B. anthracis were clustered together including Bacillus sp. Kyungjoo 3. However, the isolates of B. anthracis closely related spp. Rho, S10A, 11R1, CAU9910, CAU9911, CAU9912 and CAU9913 were clustered with the other group. On the basis of these results, isolates of B. anthracis Bongchon, Kyungjoo 1, 2 and Bacillus sp. Kyungjoo 3 were reclassified as a B. anthracis. It is concluded that FAMEs analysis provides a sensitive and reliable method for the identification of B. anthracis from closely related taxa.

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Appropriate Pretreatment Method of Coir Bag in Coir Culture (코이어 재배시 적정 전처리 방법 구명)

  • Kim, Sung Eun;Lee, Moon Haeng;Kim, Young Shik
    • Journal of Bio-Environment Control
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    • v.21 no.3
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    • pp.170-179
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    • 2012
  • We examined pretreatment methods eliminating potassium and sodium efficiently for coir bag used in hydroponics by analyzing drainage coming from coir bags. In the first experiment we investigated for six coir bags with the high market shares. The three types of pretreatment were washing coir bags with only water for 7 days (W7S0), washing with water for 4 days and further with nutrient solution for 3 days (W4S3), and washing with only nutrient solution for 7 days (W0S7). In the second experiment we tested reproducibility of the experiment results for Bio Grow and coco Mix among six coir bags used in the first experiment to verify the results. As a result, the best pretreatments for the pH stabilization were W4S3 and W0S7. The EC value of the drainage was stabilized to less than 1.0 that is the same as EC of the supply solution on the fourth day in all treatments. The nutrients of the drainage in W7S0 was stabilized in 3~4 days but calcium and magnesium were depleted. We assessed that washing longer than 4 days was waste of water. The stabilization of coir bags in W0S7 was similar to it in W4S3, but washing with the nutrient solution for 7 days seemed to be uneconomical. The reproducibility experiment for two coir bags ensured the results in the first experiment. Therefore, the pretreatment method, which is the most simple to implement and economic, seems to wash with water for 3 days and then with the nutrient solution for 1 day before planting on coir bag.

Characteristics of Runout Distance of Debris Flows in Korea (한국 토석류의 이동거리 특성)

  • Choi, Dooyoung;Paik, Joongcheol
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.32 no.3B
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    • pp.193-201
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    • 2012
  • In the last decade, heavy rainfall induced debris flow events have been remarkably occurred in Korea. Consequently, debris flow is becoming one of the most dangerous natural phenomena in mountainous area. Understanding and correct predicting of the runout distance of debris flow is an essential prerequisite for developing debris flow hazard map and prevention technology. Based on the simple and widely used sled model, in this study, we analyse the net efficiency of debris flows which is a dimensionless constant (=1/R) and defined by the ratio of the horizontal runout distance L from the debris flow source to deposit and the vertical elevation H of the source above the deposit. The analysis of field data observed in total 238 debris flow events occurred from 2002 to 2011 reveals that the representative value of the net efficiency of debris flows in Korea is 4.3. The data observed in Gangwon province where is the most debris flow-prone area in Korea shows that debris flows in Inje area have the runout distance longer than those in Pyongchang and Gangneung. Overall features of the net efficiency of debris flows observed in the central Korea are similar to those in the southern Korea. The estimation based on aerial photographs and available depositional conditions appears to overestimate the net efficiency compared to estimation based on the field observations, which indicates that appropriate depositional conditions need to be developed for debris flows in Korea.

Modification of Indophenol Reaction for Quantification of Reduction Activity of Nanoscale Zero Valent Iron (나노 영가철 환원 반응성의 정량 분석을 위한 수정된 인도페놀법 적용)

  • Hwang, Yuhoon;Lee, Wontae;Andersen, Henrik R.
    • Journal of Korean Society of Environmental Engineers
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    • v.38 no.12
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    • pp.667-675
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    • 2016
  • Nanoscale zero-valent iron (nZVI) has been effectively applied for environmental remediation due to its ability to reduce various toxic compounds. However, quantification of nZVI reactivity has not yet been standardized. Here, we adapted colorimetric assays for determining reductive activity of nZVIs. A modified indophenol method was suggested to determine reducing activity of nZVI. The method was originally developed to determine aqueous ammonia concentration, but it was further modified to quantify phenol and aniline. The assay focused on analysis of reduction products rather than its mother compounds, which gave more accurate quantification of reductive activity. The suggested color assay showed superior selectivity toward reduction products, phenol or aniline, in the presence of mother compounds, 4-chlorophenol or nitrobenzene. Reaction conditions, such as reagent concentration and reaction time, were optimized to maximize sensitivity. Additionally, pretreatment step using $Na_2CO_3$ was suggested to eliminate the interference of residual iron ions. Monometallic nZVI and bimetallic Ni/Fe were investigated with the reaction. The substrates showed graduated reactivity, and thus, reduction potency and kinetics of different materials and reaction mechanism was distinguished. The colorimetric assay based on modified indophenol reaction can be promises to be a useful and simple tool in various nZVI related research topics.

Comparisons of Urinary Arsenic Analysis by Pre-reductant for Preconditioning via the FI-HG-AAS Method (FI-HG-AAS를 이용한 전처리 과정에서 사용되는 예비환원제의 종류에 따른 요중 비소 분석결과 비교)

  • Choi, Seung-Hyun;Choi, Jae Wook;Cho, YongMin;Bae, Munjoo
    • Journal of Environmental Health Sciences
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    • v.41 no.5
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    • pp.289-298
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    • 2015
  • Objectives: The method of analyzing urinary arsenic by flow injection hydride generation atomic absorption spectrometry (FI-HG-AAS) is generally used because it shows relatively greater sensitivity, low detection limits, low blocking action, and is simple to operate. In this study, the results of analysis according to three pre-reductants commonly used in the FI-HG-AAS method were compared with each other. Methods: To analyze urinary arsenic, nineteen urine samples were collected from adults aged 43-79 years old without occupational arsenic exposure. Analysis equipment was FI-HG-AAS (AAnalyst 800/FIAS 400, Perkin- Elmer Inc., USA). The three pre-reductants were potassium iodide (KI/AA), C3H7NO2S (L-cysteine), and a mixture of KI/AA and L-cysteine (KI/AA&L-cysteine). Results: In the results of the analysis, the recovery rate of the method using KI/AA was 82.3%, 95.7% for Lcysteine, and 123.5% for KI/AA and L-cysteine combined. When compared with the results by use of high performance liquid chromatography inductively-coupled plasma mass spectrometry (HPLC-ICP-MS), the method using L-cysteine was the closest to those using HPLC-ICP-MS ($98.57{\mu}g/L$ for HPLC-ICP-MS; $74.96{\mu}g/L$ for L-cysteine; $69.23{\mu}g/L$ for KI/AA and L-cysteine; $13.06{\mu}g/L$ for KI/AA) and were significantly correlated (R2=0.882). In addition, they showed the lowest coefficient of variation in the results between two laboratories that applied the same method. Conclusion: The efficiency of hydride generation is considered highly important to the analysis of urinary arsenic via FI-HG-AAS. This study suggests that using L-cysteine as a pre-reductant may be suitable and the most rational among the FI-Hg-AAS methods using pre-reductants.