• Proceedings of the Korean Society of Sericultural Science Conference
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• 2002.10a
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• pp.43-43
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• 2002

No Abstract, See Full Text

• International Journal of Industrial Entomology and Biomaterials
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• v.6 no.1
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• pp.27-31
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• 2003

Infectious flacherie of silkworm Bombyx mori is caused by B. mori infectious flacherie virus (BmIFV) and causes severe crop loss to sericulturists. In the present study, a colloidal textile dye-based dipstick immunoassay is developed for the detection of infectious flacherie in silkworms. Colloidal textile dye (blue D2R) with Aλ$_{max}$ at 620 nm was sensitised with 500 $\mu\textrm{g}$/ml of purified anti-BmIFV IgG. The dye-antibody reagent detects purified antigen up to 10 ng/ml and BmIFV infection in diseased larval extracts $(up to a dilution of {10^-5})$ and faecal matter extracts $(up to a dilution of {10^-2})$ by forming clear blue dot within 30 min. It was observed to be stable for three months period at $4^{\circ}C$. The efficacy of textile dye-based dipstick immunoassay was on pay with HRP-based dipstick immunoassay and fluorescent antibody test, and better than latex agglutination and ouchterlony tests in the detection of BmIFV The dye-based dipstick immunoassay method provides a simple, sensitive and less expensive test for the detection of BmIFV infection in silkworms.s.

• Journal of Sericultural and Entomological Science
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• v.4
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• pp.47-50
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• 1965

It is well known that the mid gut polyhedroses can be a cause of serious disease of silkworm in Japan. In our country, however, this disease has not been discussed up to the present. Author made the experiments to know whether this disease aws infectious or not in our country. The results are as follows: 1. The mid-gut polyhedroses was induced by some artificial treatments, of which the most effective one was low temperature treatment (at 5$^{\circ}C$ for 24 hours) at the 5th instar before first feeding. 2. Author found a few infected silkworms which were reared by a farmer in fall in 1964. It was supposed that the induction of this disease was due to the cumulative effects of the bad conditions on the silkworms. Especially the great cause of this disease depended on the quality of the mulberry leaves.

• Journal of Sericultural and Entomological Science
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• v.43 no.1
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• pp.9-15
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• 2001

New Mulberry Cultivar, Milsungppong, was selected from F1 seedlings obtained by crossing the female, 4X Keomseolppong, and the male, Kaeryangppong. Local adaptability test has been carried out at five places(Suwon, Chunchun, Cheongju, Kongju and Sangju) for four years since 1992. Leaf quality was tested by silkworm rearing for two silkworm rearing seasons(spring and autumn) at Suwon in 1995. From these tests, Milsungppong was found to be superior to control cultivar, cultivar, Kaeryangppong, in productivity, rootability and shoot cold injury. The new cultivar, Milsungppong, has following agronomic characteristics as compared with Kaeryangppong, the most prevailing cultivar, Milsungppong, has following agronomic characteristics as compared with Kaeryangppong, the most prevailing cultivar in Korea It is triploid cultivar belonging to Morus bombycis KOIDZ with the leaf shape of five lobes, sprouting in spring nearly the same to the Kaeryangppong (Morus alba L.), high leaf yielding of 25% compared to Kaeryangppong (Morus alba L.) and the medium degree of cold resistance. It is adaptable to every where in Korea except the area cold injury broken out habitually. Milsungppong is recommended to rear silkworms not only in spring but also in autumn besides for young silkworms.

• The Korean Journal of Pesticide Science
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• v.13 no.3
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• pp.177-184
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• 2009

This study was carried out to investigate infection process, symptoms and main component of the domestic silkworm, Bombyx mori. larvae and pupa infected with the entomopathogenic fungus Cordyceps, Paecillomyces tenuipes. The Cordyceps, Paecillomyces tenuipes, was highly infectious to the silkworms. A pathogenicities of Cordyceps, Paecillomyces tenuipes, may be highly virulent because of the low resistance or high susceptibility of the silkworms. The silkworm larva infected with Cordyceps formed phialospores on the phialides at the imperfect stage of the genus Cordyceps, But silkworm pupa infected with Cordyceps formed ascospores in the asci at the perfection stage of the genus Cordyceps. The results of analysis of health silkworm pupa and silkworm pupa infected with Cordyceps were obtained that amino acid, fatty acid, and nucleoside were very different.

• Journal of Sericultural and Entomological Science
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• v.40 no.2
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• pp.117-125
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• 1998

Entomopathogenic nematodes, Heterorhabditidae and Steinernematidae, were isolated from the soil of mulberry field, and the high infectivity and invesiveness were confirmed in the silkworm, Bombyx mori. The cause of non-microbial and acute flacherie was found as an disease by infection with soil-born nematodes through the mulberry leaves contaminated with soil and rainwater. The causal nematodes were isolated by silkworm trap from all of the 5 soil samples collected on the 5 mulberry fields, and identified as 3 strains of Heterorhabditis sp. and 2 of Steinernema sp. Rainwater itself, however, wasn't engaged in the silkworm disease, mulberry leaves with rainwater was rather profitable for cocoon production when the leaf quality was too hard to feed silkworm. Feeding of wet mulberry leaves with rain might not so harm to silkworm when the condition of rearing room to be kept at suitable temperature and ventilated well. Nematode infection of silkworm could be occurred by harvesting and feeding of contaminated mulberry leaves on the weather condition of rainy and wind. For the prevention of nematode infection, silkworms should be fed the leaves harvested from the higher portion of the mulberry tree in rainy days. For an oppositional application of this susceptibility of silkworms to nematode, might be useful on the collection and amplification of nematode agents for biotic control of pest insects.

• International Journal of Industrial Entomology and Biomaterials
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• v.22 no.1
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• pp.21-23
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• 2011

In order to produce the colored cocoons we finalized the adequate amount of dyes throughout several trial and error experiments. The proper amount of each dye required for per 100 g of each colored cocoon turned out to be 150 mg of Rhodamine, 1560 mg of Thionin, 170 mg of Neutral red, and 200 mg of N-Blue, respectively. With this amount of dyes silkworms grew without physiological disorders. In order to produce colored cocoons, artificial diet composed mainly of mulberry leaves was fed to silkworms from the beginning of 5th instar, and subsequently fed with dye included diet from $4^{th}$ day of the 5th instar. This process resultantly produced colored silkworm body from the onset of feeding and subsequently colored cocoons and eggs. Nevertheless, the dye induced color was not inherited to next generation.

• Journal of Sericultural and Entomological Science
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• v.28 no.1
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• pp.54-60
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• 1986

Silkworms have been found cross infected with other microsporidia of insects in mulberry trees, forest and fruit gardens. Even the unidentified microsporidian species were not seriously pathogenic to silkworms, the silkworm egg producers lose their profit because of the elimination of eggs laid from moths which are infected with any kind of microsporidian species. Recently, the microsporidian cross infection to silkworm is in tendency of increase and the authors have investigated the field insects to examine the microsporidia. The number of species of insects infected with microsporidia was 10 and they were Boettcherisca peregrina (Robineau-Dewvoidy), Apis melifera linnaeus, Artogenia rapae Linnaeus, Tipula aino Alexander, Altica cacrulescens (Baly), Anomela daimiana Harold, Eilema griseola (Jubner), Rbalbistylun speciosum Uller, Anisodactylus signatus illiger, Oulema oryzae (Kuwayama). From the Boettcherisca peregrina (Rogineau-Desvoidy), three different species of micrsporidia were isolated and the microsporidia isolated from Boettcherisca peregrina (Robineau-Desvoidy), Apis melifera Linnaeus, Artogenia rapae Linnaeus donot have infectivity to silkworm larvae, Bombyx mori L.

• Biomedical Science Letters
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• v.28 no.4
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• pp.322-326
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• 2022

The purpose of this study was to develop hydrolysate from silkworm (Cudrania Silkworm Fibroin Peptide; CSFP), a food containing components for improving blood vessel health. In general, Cudrania tricuspidata leaves contain about 5 times more rutin and 25 times more dihydroquercetin than mulberry leaves. 1-Deoxynojirimycine (1-DNJ), dihydroquercetin and rutin inhibit the activity of carbohydrate-digesting enzymes, inhibit blood lipid peroxidation, and regulate insulin secretion, which helps blood vessels to be healthy. When the diet-controlled silkworm was enzymatically hydrolyzed, it was confirmed that rutin content was about 8 times higher than that of the in general silkworm as a control. In the silkworm hydrolysate, CSFP, developed as a final food material, the active ingredients were 65 mg/kg for rutin, 3,328 mg/kg for DNJ, 0.43 mg/kg for dihydroquercetin, and 82,624 mg/kg for total polyphenol, which was confirmed through LC-MS/MS analysis. In conclusion, it was found that silkworms fed with C. tricuspidata leaves as a diet had more active components that can help control blood sugar and improve blood vessel health than silkworms fed with mulberry leaves.

• Korean Journal of Pharmacognosy
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• v.54 no.1
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• pp.38-43
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• 2023

A simple and accurate assay was developed for the quantitative analysis of 1-deoxynojirimycin (1-DNJ) derived from the silkworm (Bombyx mori). Normal-phase high-performance liquid chromatography coupled with an evaporative light scattering detector (HPLC-ELSD) and a hydrophilic interaction liquid chromatography column was used. Various parameters were applied to optimize the analysis method. The limits of detection and quantification of 1-DNJ were 2.97 × 10^-3 and 9.00 × 10^-3 mg/mL, respectively. The calibration curve showed good linearity results. The concentration range and the r² value were 0.0625-1.0 mg/mL and 0.9997, respectively. The accuracy test demonstrated a significantly high recovery rate (89.95-103.22%). The relative standard deviation was ≤ 1.00%. Thus, a method for the accurate identification and quantitative analysis of 1-DNJ in silkworms was developed. Moreover, in this procedure, the process of derivatization of 1-DNJ, which was required in previous experiments, could be eliminated. This technique may be actively utilized for the development of pharmaceuticals and health functional foods using 1-DNJ.