• 한국생물공학회:학술대회논문집
• /
• 2003.10a
• /
• pp.313-313
• /
• 2003

• 한국생물공학회:학술대회논문집
• /
• 2003.04a
• /
• pp.102-102
• /
• 2003

• International Journal of Industrial Entomology and Biomaterials
• /
• v.2 no.2
• /
• pp.149-153
• /
• 2001

The influence of baculovirus Bombyx mori Nuclear Polyhedrosis virus (BmNPV) infection on intermediary metabolic pathways in silkworm Bombyx mori L. was investigated. Studies revealed that NADP-linked malate dehydrogenase activity in hemolymph of infected silkworms at 96 hrs post infection (p.i.) with visible symptoms of infection was enhanced in comparison to healthy larvae of the same age. Also, NADP-dependent MDH activity was significantly lower in fat body cytosol of infected larvae at 96 hrs p.i. when compared to healthy larvae. Similarly, some biometabolic parameters like growth, protein content and cholesterol titer were observed to be influenced by baculovirus infection. While the growth of infected larvae was significantly retardedi protein content was also drastically reduced in both hemolymph and fat body tissues. Cholesterol titers however, was enhanced in infected larvae. The results observed herein point to a significant change in the normal biochemical and biometabolic pathways required for growth and development following BmNPV infection.

• Journal of Sericultural and Entomological Science
• /
• v.23 no.1
• /
• pp.38-46
• /
• 1981

In order to investigate a suitable carbohydrate-resources and the activities of starch decomposing enzymes in artifical diet of silkworm, the experiment was undertaken by adding eight kinds of starch in the diet of silkworm. Major characters and zymograms of amylase in body organs were studied by electrophoresis. The results obtained are as follows: 1. Starches of rice, barey and millet were comparatively good for maintaining practical characters of silkworm. 2. It is assumed that no treatments are need to increas purity of starch resources for artificial diet of silkworm. It was found that starch amounts adding to artificial diet are moeerate ranging 12 to 18 percent as dry weight. 3. Regardless of kinds of starch and varieties of silkwormr sametype of electrophoresis zymogram for amylase was resulted as three bands in hemolymph, four bands in intestine and two bands in intestine and two bands in silkgland. There was no band in the digestive juice. In case of 18 percent addition of starch and check plots, no amylose change was investigated in the hemolymph.

• Journal of Sericultural and Entomological Science
• /
• v.39 no.2
• /
• pp.119-133
• /
• 1997

Isozyme was used to characterize general protein patterns of genetic relationships among 303 silkworm stocks preserved in National Sericultural and Entomology Research Institute, RDA. Six isozymes (esterase, acid phosphatase, alkaline phosphatase, amylase, glucose-6-phosphate dehydrogenase and sucrase) from hemolymph, midgut, and digestive juice were employed to construct dendograms(UPGMA method) using a polycrylamide gel electrophoresis. A cluster analysis revealed four major group, which were divided into several subgroups within each group, contained assemglages of Japanese and Chinese races. Especially, genetic differentiation in the first and second group was greatest rather than within Japanese and Chinese races repectively and was concordant with the hypothesis of phyletic sorting of initial variability in China many years ago. Hypothesized recent introgression between groups was also plausible, but the eviednce suggested bidirectional gene flow between the Chinese and the Japnaese lineages. Interpreting the results in light of evidence from the current study, the genetic diversity and relationship showed in Korean silkworm race, Hansammyun reflected early and independent evolution from the Chinese ancestor, limited addition of new variability and phyletic sorting within Korean peninsula more than 4,000 years.

• Proceedings of the Korean Society of Sericultural Science Conference
• /
• 2001.04a
• /
• pp.38-38
• /
• 2001

No abstract. See Full-text

• 한국생물공학회:학술대회논문집
• /
• 2005.10a
• /
• pp.388-388
• /
• 2005

• BMB Reports
• /
• v.38 no.6
• /
• pp.717-724
• /
• 2005

The nontoxic B subunit of cholera toxin (CTB) can significantly increase the ability of proteins to induce immunological tolerance after oral administration, when it was conjugated to various proteins. Recombinant CTB offers great potential for treatment of autoimmune disease. Here we firstly investigated the feasibility of silkworm baculovirus expression vector system for the cost-effective production of CTB under the control of a strong polyhedrin promoter. Higher expression was achieved via introducing the partial non-coding and coding sequences (ATAAAT and ATGCCGAAT) of polyhedrin to the 5' end of the native CTB gene, with the maximal accumulation being approximately 54.4 mg/L of hemolymph. The silkworm bioreactor produced this protein vaccine as the glycoslated pentameric form, which retained the GM1-ganglioside binding affinity and the native antigenicity of CTB. Further studies revealed that mixing with silkworm-derived CTB increases the tolerogenic potential of insulin. In the nonconjugated form, an insulin : CTB ratio of 100 : 1 was optimal for the prominent reduction in pancreatic islet inflammation. The data presented here demonstrate that the silkworm bioreactor is an ideal production and delivery system for an oral protein vaccine designed to develop immunological tolerance against autoimmune diabetes and CTB functions as an effective mucosal adjuvant for oral tolerance induction.

• KSBB Journal
• /
• v.10 no.3
• /
• pp.317-322
• /
• 1995

Insect cells were grown and infected with baculovirus for the production of recombinant protein. Later infection gave the lower expression of recombinant protein. This indicates that the expression rate is lower at higher cell concentration. This phenomena provides a well-posed optimization problem with respect to the infection time. The optimal infection time was experimentally shown to exist for the maximum productivity of recombinant protein. Also, the expression increased with the addition of 5% silkworm hemolymph. This is considered to be due to the increase of intracellular viruses and the longevity of viable cells after the infection. The production of ${\beta}$-galaclosidase increased about ten-fold with the addition of yeastolate and silkworm hemolymph for high cell density and high expression, respectively.

• Journal of Sericultural and Entomological Science
• /
• v.13 no.2
• /
• pp.141-143
• /
• 1971

By means of thin-layer electrophoresis in agarose gel, hemolymph protein of healthy silkworm larvae and of the nuclear polygedrosis virus infected larvae were studied. 1. In the 4th instar, 4 fractions moving toward anode were separated. Dye-binding Capacity of the fraction was increased according to the stage. 2. After 5th day in the 5th instar, 7 fractions moving toward anode were separated, and one fraction toward cathode was separated. 3. On the first day in the 5th instar, 5 fractions were separated, and on the 4th day of the same instar 5 fractions were separated. 4. As for the hemolymph protein fractions of the polyhedrosis virus infected larvae, on the 6th and 7th day, three fractions(D.E.F) were inclined to increase, whereas on the 8th day 4 fractions(A.B.D.E) were disappeared but F fraction was inclined to decrease.