• Geomechanics and Engineering
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• 제18권5호
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• pp.545-554
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• 2019

The customary approach used in the blast vibration analysis is to derive empirical relations between the peak particle velocities of blast-induced waves and the scaled distance, and to develop patterns limiting the amounts of explosives. During the periods when excavations involving blasting were performed at sites far from residential areas and infrastructure works, this method based on empirical correlations could be effective in reducing vibrations. However, blasting procedures applied by the fast-moving mining and construction industries today can be very close to, in particular cities, residential areas, pipelines, geothermal sites, etc., and this reveals the need to minimize blast vibrations not only by limiting the use of explosives, but also employing new scientific and technological methods. The conventional methodology in minimizing blast vibrations involves the steps of i) measuring by seismograph peak particle velocity induced by blasting, ii) defining ground transmission constants between the blasting area and the target station, iii) finding out the empirical relation involving the propagation of seismic waves, and iv) employing this relation to identify highest amount of explosive that may safely be fired at a time for blasting. This paper addresses practical difficulties during the implementation of this conventional method, particularly the defects and errors in data evaluation and analysis; illustrates the disadvantages of the method; emphasizes essential considerations in case the method is implemented; and finally discusses methods that would fit better to the conditions and demands of the present time compared to the conventional method that intrinsically hosts the abovementioned disadvantages.

• 화약ㆍ발파
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• 제23권2호
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• pp.57-66
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• 2005

A major concern with blasting at surface mines is generation of ground vibration, air blast, flyrock, dust & fume and their impact on nearby structures and environment. A study was conducted at a coal mine in India which produces 10 million tonne of coal and 27 million cubic meter of overburden per annum. Draglines and shovels with dumpers carry out the removal of overburden. Detonation of 100 tonnes of explosives in a blasting round is a common practice of the mine. These large sized blasts often led to complaints from the nearby inhabitants regarding ground vibrations and their affects on their houses. Eighteen dragline blasts were conducted and their impacts on nearby structures were investigated. Extended seismic arrays were used to identify the vibration characteristics within a few tens meters of the blasts and also as modified by the media at distances over 5 km. 10 to 12 seismographs were deployed in an array to gather the time histories of vibrations. A signature blast was conducted to know the fundamental frequency of the particular transmitting media between the blast face and the structures. The faster decay of high frequency components was observed. It was also observed that at distances of 5km, the persistence of vibrations in the structures was substantially increased by more 10 seconds. The proximity of the frequency of the ground vibration to the structure's fundamental frequencies produced the resonance in the structures. On the basis of the fundamental frequency of the structures, the delay interval was optimized, which resulted into lower amplitude and reduced persistence of vibration in the structures.

• 한국우주과학회:학술대회논문집(한국우주과학회보)
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• 한국우주과학회 2009년도 한국우주과학회보 제18권2호
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• pp.37.1-37.1
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• 2009

Solar type II radio burst is regarded as a signature of coronal shock. However its association with coronal mass ejections (CMEs)-driven shock and/or flare blast waves remains controversial. On December 31 2007, SOHO/LASCO and STEREO/COR observed a CME that occurred on the east limb of the Sun. Meanwhile, two type II bursts were observed sequently by KASI/E-Callisto and the Culgoora radio observatory during the CME apparence time. In this study, we estimate kinematics of the two coronal shocks from dynamic spectrum of the multiple type II bursts and compare with the kinematics of the CME derived from the space observations. An origin of the multiple type II bursts is inspected and discussed briefly.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 춘계학술대회 및 임시총회
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• pp.27-28
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• 2014

Beauveria bassiana (Cordycipitaceae, Hypocreales, Ascomycota) is an anamorphic fungus having a potential to be used as a biological control agent because it parasitizes a wide range of arthropod hosts including termites, aphids, beetles and many other insects. A number of bioactive secondary metabolites (SMs) have been isolated from B. bassiana and functionally verified. Among them, beauvericin and bassianolide are cyclic depsipeptides with antibiotic and insecticidal effects belonging to the enniatin family. Non-ribosomal peptide synthetases (NRPSs) play a crucial role in the synthesis of these secondary metabolites. NRPSs are modularly organized multienzyme complexes in which each module is responsible for the elongation of proteinogenic and non-protein amino acids, as well as carboxyl and hydroxyacids. A minimum of three domains are necessary for one NRPS elongation module: an adenylation (A) domain for substrate recognition and activation; a tholation (T) domain that tethers the growing peptide chain and the incoming aminoacyl unit; and a condensation (C) domain to catalyze peptide bond formation. Some of the optional domains include epimerization (E), heterocyclization (Cy) and oxidation (Ox) domains, which may modify the enzyme-bound precursors or intermediates. In the present study, we analyzed genomes of B. bassiana and its allied species in Hypocreales to verify the distribution of NRPS-encoding genes involving biosynthesis of beauvericin and bassianolide, and to unveil the evolutionary processes of the gene clusters. Initially, we retrieved completely or partially assembled genomic sequences of fungal species belonging to Hypocreales from public databases. SM biosynthesizing genes were predicted from the selected genomes using antiSMASH program. Adenylation (A) domains were extracted from the predicted NRPS, NRPS-like and NRPS-PKS hybrid genes, and used them to construct a phylogenetic tree. Based on the preliminary results of SM biosynthetic gene prediction in B. bassiana, we analyzed the conserved gene orders of beauvericin and bassianolide biosynthetic gene clusters among the hypocrealean fungi. Reciprocal best blast hit (RBH) approach was performed to identify the regions orthologous to the biosynthetic gene cluster in the selected fungal genomes. A clear recombination pattern was recognized in the inferred A-domain tree in which A-domains in the 1st and 2nd modules of beauvericin and bassianolide synthetases were grouped in CYCLO and EAS clades, respectively, suggesting that two modules of each synthetase have evolved independently. In addition, inferred topologies were congruent with the species phylogeny of Cordycipitaceae, indicating that the gene fusion event have occurred before the species divergence. Beauvericin and bassianolide synthetases turned out to possess identical domain organization as C-A-T-C-A-NM-T-T-C. We also predicted precursors of beauvericin and bassianolide synthetases based on the extracted signature residues in A-domain core motifs. The result showed that the A-domains in the 1st module of both synthetases select D-2-hydroxyisovalerate (D-Hiv), while A-domains in the 2nd modules specifically activate L-phenylalanine (Phe) in beauvericin synthetase and leucine (Leu) in bassianolide synthetase. antiSMASH ver. 2.0 predicted 15 genes in the beauvericin biosynthetic gene cluster of the B. bassiana genome dispersed across a total length of approximately 50kb. The beauvericin biosynthetic gene cluster contains beauvericin synthetase as well as kivr gene encoding NADPH-dependent ketoisovalerate reductase which is necessary to convert 2-ketoisovalarate to D-Hiv and a gene encoding a putative Gal4-like transcriptional regulator. Our syntenic comparison showed that species in Cordycipitaceae have almost conserved beauvericin biosynthetic gene cluster although the gene order and direction were sometimes variable. It is intriguing that there is no region orthologous to beauvericin synthetase gene in Cordyceps militaris genome. It is likely that beauvericin synthetase was present in common ancestor of Cordycipitaceae but selective gene loss has occurred in several species including C. militaris. Putative bassianolide biosynthetic gene cluster consisted of 16 genes including bassianolide synthetase, cytochrome P450 monooxygenase, and putative Gal4-like transcriptional regulator genes. Our synteny analysis found that only B. bassiana possessed a bassianolide synthetase gene among the studied fungi. This result is consistent with the groupings in A-domain tree in which bassianolide synthetase gene found in B. bassiana was not grouped with NRPS genes predicted in other species. We hypothesized that bassianolide biosynthesizing cluster genes in B. bassiana are possibly acquired by horizontal gene transfer (HGT) from distantly related fungi. The present study showed that B. bassiana is the only species capable of producing both beauvericin and bassianolide. This property led to B. bassiana infect multiple hosts and to be a potential biological control agent against agricultural pests.