• Title/Summary/Keyword: signals

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Cytogenetic Study of Maackia amurensis Rupr. & Maxim. and M. fauriei (Levl.) Takeda Using Karyotyping Analysis and the FISH Technique (핵형분석과 FISH 기술을 이용한 솔비나무와 다릅나무의 세포유전학적 연구)

  • Kim, Soo-Young;Kim, Chan-Soo
    • Korean Journal of Plant Taxonomy
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    • v.39 no.3
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    • pp.193-198
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    • 2009
  • Chromosome analysis using karyotyping and bicolor FISH were carried out for two Maackia species (M. fauriei and M. amurensis) found in Korea. The somatic metaphase chromosome number was 2n = 2x = 18 in both, and the size of these chromosomes ranged from 3.58 to $5.82{\mu}m$. The chromosome complements consisted of two pairs of metacentric (chromosomes 1 and 7), four pairs of submetacentrics (chromosomes 4, 6, 8 and 9) and three pairs of subtelocentrics (chromosomes 2, 3 and 5) in M. fauriei but, chromosomes 4 (subtelocentric) and 7 (submetacentric) of M. amurensis have different morphology. Using bicolor FISH, a pair of 45S rDNA loci were observed for both M. fauriei and M. amurensis, but the number and site of the 5S rDNA signal were different in the two species. M. fauriei has two pairs of 5S signals on chromosomes 7 and 8 but, M. amurensis has four paris on chromosomes 3, 4, 7 and 7. Hence, the 5S rDNA is a useful FISH for Maackia species.

Utilization of Multiple Carbon Sources by Plant Cells

  • Lee, Taek-Kyun;Suh, Jung-Bin;Kim, Se-Hee;Lee, Sun-Min;Lee, Woo-Sung
    • Proceedings of the Zoological Society Korea Conference
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    • 1999.10b
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    • pp.11-11
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    • 1999
  • ;It has been reported that suspension-cultured rice cells grown on mixed carbon sources of glucose (GIc) and acetate exhibited diauxic growth in which acetate was the preferred carbon source (Lee and Lee, 1996). Carrot (Daucus carota L.) suspension cells, showing a diauxic growth very similar to that of rice cells, were used to delineate the mechanisms underlying this preferential use of acetate over GIc. Uptakes of both GIc and 3-0-methylglucose (3-0MG), a non-metabolizable GIc analogue, were similarly inhibited when acetate or butylate, weak acids which are capable of transporting protons into the cytosol, were present in the uptake assay mixture containing cells harvested during the GIc-utilizing second growth phase. Inhibition of GIc uptake by these weak acids was similar when equivalent experiments were carried out with isolated plasma membranes. It was further shown that Glc uptake, which requires a proper proton gradient across the plasma membranes, was inhibited during the first growth phase by acetate-mediated alkalization of growth medium and/or simultaneous acidification of cytosol. This study strongly suggests that Glc utilization in plant cells is inhibited by co-presenting carbon source(s) which can alter the proton gradient across the plasma membrane. We further examined diauxic growth in culture containing GIc and malate. Unlike the case in the culture with GIc and acetate, carrot cells used GIc first. Malate was utilized only after Glc is depleted from medium. These results indicate that GIc can be a preferred or less-preferred carbon source depending on the competing carbon source. It was noted that malate was not directly taken up by cells. Instead it was converted extracellularly into fumarate which was subsequently transported into cells. During the malate-growth phase malate uptake was negligible, and fumarate uptake was active and pH-sensitive. It was shown that fumarase released into medium was responsible for the extracellular conversion of malate into fumarate. An immunoblot experiments showed that fumarase antibody raised against Arabidopsis fumarase provided positive signals only in medium in malate culture, not in fumarate or GIc cultures. This study demonstrates the first example in that fumarase, a mitochondria marker enzyme, can be present in places other than mitochondria.ndria.

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Differences in the Electronic Structures of Bulk and Powder FeV2O4 Spinel Oxide Investigated by Using Synchrotron Radiation (방사광을 이용한 FeV2O4 스피넬 산화물의 덩치상태와 분말상태의 전자구조 차이 연구)

  • Hwang, Ji-Hoon;Kim, D.H.;Lee, Eun-Sook;Kang, J.S.;Kim, W.C.;Kim, C.S.;Han, S.W.;Hong, S.C.;Park, B.G.;Kim, J.Y.
    • Journal of the Korean Magnetics Society
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    • v.21 no.6
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    • pp.198-203
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    • 2011
  • The electronic structure of ferrimagnetic spinel oxide of $FeV_2O_4$ has been investigated by employing soft x-ray absorption spectroscopy (XAS) and soft x-ray magnetic circular dichroism (XMCD). The Fe 2p and V 2p XAS spectra show that the valence states of Fe and V ions are ${\sim}Fe^{2.3+}$ mixed-valent states and ${\sim}V^{3+}$ states, respectively. In Fe 2p XMCD spectra, finite XMCD signals are observed for divalent $Fe^{2+}$ states only, but not for $Fe^{3+}$ states. This finding indicates that the magnetic moments of $Fe^{2+}$ ions are ordered ferromagnetically but that those of $Fe^{3+}$ ions are cancelled, implying that $Fe^{2+}$ ions play an important role in determining magnetic properties of $FeV_2O_4$.

A Study on Accuracy of Position Fixes Obtained by GPS at Three Fixed Stations (정점(定點)에 있어서 GSP의 측위정도(測位精度))

  • Cho, Eul-Je
    • Journal of Fisheries and Marine Sciences Education
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    • v.3 no.2
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    • pp.19-28
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    • 1991
  • The United States has been developing a GPS(Global Positoning System), and now we can make use of it everywhere in the world. The author measured the usable time and took position fixes from three fixed stations in Japan in order to evaluate the positioning accuracy of GPS, firstly by the difference in the time of the year and, secondly by the difference in location between the stations in 1988. I was able to receive positioning signals from only 6 or 7 satellites, but in July 1991, 14 GPS satellites became available. The results obtained are summerized as follows: 1) The usable time was 7~9 hours from only 6~7 satellites. 2) In the case of the former, the time zone of position fixes varied with the time of the year and there were a little differences of the accuracy of position fixes except 3H(three dimensiomal high level positioning). In the case of the latter, there were not obvious locality differences in 3D(three dimensional positioning). But the positioning errors and number of data varied on each level in 2D(two dimensiomal positioning), although the positioning errors were smaller than 0.1 nautical mile. 3) Th standard deviations of Dep(departure) were larger than tat of D.lat(difference of latitude), and varied widely to the east and west. But the standard deviations were smaller than 100 meters.

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Random heterogeneous model with bimodal velocity distribution for Methane Hydrate exploration (바이모달 분포형태 랜덤 불균질 매질에 의한 메탄하이드레이트층 모델화)

  • Kamei Rie;Hato Masami;Matsuoka Toshifumi
    • Geophysics and Geophysical Exploration
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    • v.8 no.1
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    • pp.41-49
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    • 2005
  • We have developed a random heterogeneous velocity model with bimodal distribution in methane hydrate-bearing Bones. The P-wave well-log data have a von Karman type autocorrelation function and non-Gaussian distribution. The velocity histogram has two peaks separated by several hundred metres per second. A random heterogeneous medium with bimodal distribution is generated by mapping of a medium with a Gaussian probability distribution, yielded by the normal spectral-based generation method. By using an ellipsoidal autocorrelation function, the random medium also incorporates anisotropy of autocorrelation lengths. A simulated P-wave velocity log reproduces well the features of the field data. This model is applied to two simulations of elastic wane propagation. Synthetic reflection sections with source signals in two different frequency bands imply that the velocity fluctuation of the random model with bimodal distribution causes the frequency dependence of the Bottom Simulating Reflector (BSR) by affecting wave field scattering. A synthetic cross-well section suggests that the strong attenuation observed in field data might be caused by the extrinsic attenuation in scattering. We conclude that random heterogeneity with bimodal distribution is a key issue in modelling hydrate-bearing Bones, and that it can explain the frequency dependence and scattering observed in seismic sections in such areas.

Evaluation of Cell Viability and Delivery Efficiency in Electroporation System According to the Concentrations of Propidium Iodide and Yo-Pro-1 (전기천공시스템에서 Propidium Iodide와 Yo-Pro-1의 농도에 따른 세포 생존율과 전달효율 평가)

  • Bae, Seo Jun;Im, Do Jin
    • Korean Chemical Engineering Research
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    • v.57 no.6
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    • pp.898-906
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    • 2019
  • In this study, basic research was conducted to provide guidelines for selecting fluorescent dye and using proper concentration of fluorescent dye to use evaluation of cell viability and fluorescent dye delivery efficiency. Propidium iodide and Yo-Pro-1 were used as fluorescent dyes. In the evaluation of cell viability and the efficiency of delivery using Propidium Iodide and Yo-Pro-1, the histogram of each fluorescent dye was different depending on the type of fluorescent dye and the concentration used. These results were related to the characteristics of the fluorescent dyes used. This was related to the properties of the fluorescent dyes used. From these results, it was found that the analytical results depending on the characteristics of the fluorescent dyes used in the cell analysis. The effect of the fluorescent dye on the cell was confirmed, but it was confirmed that it did not affect the analysis result. In addition, the influence of interference between fluorescent signals was confirmed when two or more kinds of fluorescent dyes were used for analysis. The higher the concentration of Yo-Pro-1 was, the larger the effect of interference was, and the concentration of Propidium Iodide did not affect the interference of fluorescence signal. This study confirmed that the evaluation of the cell viability and the evaluation of the delivery efficiency were influenced by the type and concentration of the fluorescent dyes and it was related to the characteristics of the fluorescent dyes. Based on the results, appropriate concentrations of fluorescent dyes suitable for evaluation of cell viability and delivery efficiency were suggested.

Convergence Study of Brain Activity by Dominant Hand Using functional near-infrared spectroscopy(fNIRS) (기능적 근적외선 분광법(fNIRS)을 이용한 우세손에 따른 뇌 활성화도에 대한 융합 연구)

  • Kim, Mi Kyeong;Park, Sun Ha;Park, Hae Yean
    • Journal of the Korea Convergence Society
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    • v.12 no.12
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    • pp.323-330
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    • 2021
  • In this study, we intended to examine the difference in brain activation due to dominant and non-dominant hands using functional near-infrared spectroscopy(fNIRS) in 10 healthy adults. Box & Block Test(BBT) was conducted under two conditions: dominant hand and non-dominant hand. During the experiment, brain activity was measured using fNIRS and signals were analyzed using nirsLAB v2019.04 software after the experiment was completed. As a result, 6 out of 10 people showed activation of the cerebral hemisphere related to the dominant hand, and only 3 out of 10 people showed activation of the cerebral hemisphere related to the non-dominant hand. In other words, both dominant and non-dominant hand cconfirmed that the cerebral hemispheres related to dominant hands were more active. Therefore, it is believed that fNIRS can be used as a fundamental data applicable to children with sensory processing disorders that are difficult to identify dominant hand.

Research on Broadband Signal Processing Techniques for the Small Millimeter Wave Tracking Radar (소형 밀리미터파 추적 레이더를 위한 광대역 신호처리 기술 연구)

  • Choi, Jinkyu;Na, Kyoung-Il;Shin, Youngcheol;Hong, Soonil;Park, Changhyun;Kim, Younjin;Kim, Hongrak;Joo, Jihan;Kim, Sosu
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.21 no.6
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    • pp.49-55
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    • 2021
  • Recently, a small tracking radar requires the development of a small millimeter wave tracking radar having a high range resolution that can acquire and track a target in various environments and disable the target system with a single blow. Small millimeter wave tracking radar with high range resolution needs to implement a signal processor that can process wide bandwidth signals in real time and meet the requirements of small tracking radar. In this paper, we designed a signal processor that can perform the role and function of a signal processor for a small millimeter wave tracking radar. The signal processor for the small millimeter wave tracking radar requires the real-time processing of input signal of OOOMHz center frequency and OOOMHz bandwidth from 8 channels. In order to satisfy the requirements of the signal processor, the signal processor was designed by applying the high-performance FPGA (Field Programmable Gate Array) and ADC (Analog-to-digital converter) for pre-processing operations, such as DDC (Digital Down Converter) and FFT (Fast Fourier Transform). Finally, the signal processor of the small millimeter wave tracking radar was verified via performance test.

Design and fabrication of Ka-band high-power, high-efficiency spatial combiner using TM01 mode Transducer (TM01 모드 변환을 이용한 Ka 대역 고출력 고효율 공간 결합기 설계 및 제작)

  • Kim, Hyo-Chul;Cho, Heung-Rae;Lee, Ju-Heun;Lee, Deok-Jae;An, Se-Hwan;Lee, Man-Hee;Joo, Ji-Han;Kim, Hong-Rak
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.21 no.6
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    • pp.25-32
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    • 2021
  • In this study, it proposes a mode converter that is relatively easy to implement and can shorten the transmission line length of the final combining port and it was fabricated and tested by applying it to an 8-way spatial combiner. The proposed mode converter converts the signal converted from the doorknob-shaped circular disk connected to the ground into the TM01 mode by opening it in the circular waveguide. The 8-way waveguide spatial combiner is designed and implemented so that 8 signals input from the H-plane are combined in a circular waveguide at the center, and the final combining mode is TM01. The test results confirmed excellent performance with an insertion loss of less than 0.4dB and a combining efficiency of 95% or more. In addition, it was confirmed that it is suitable for high output by calculating the breakdown voltage and discharge threshold power of the new mode conversion structure through electric field analysis. The results confirmed through this study are expected to be applicable to high-power, high-efficiency SSPA in various fields in the future.

Interaction Design Study of Virtual Reality Safety Education Contents (가상현실 안전교육 콘텐츠의 인터랙션 디자인 연구)

  • Chang, Hyo-Jin;Chang, Sun-Hee
    • The Journal of the Korea Contents Association
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    • v.21 no.9
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    • pp.75-87
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    • 2021
  • The purpose of this study is to derive the characteristics of interaction design for each stage of content composition so that it can be referred to in the planning and production of virtual reality safety education contents. It was confirmed that each of the following interaction design features was found in the three configuration steps: acquisition of situation response procedure knowledge, accident situation experiential learning, and content confirmation and evaluation. First, it was revealed that the quality of experience was controlled by increasing the fidelity of behaviors and reducing general and repetitive behaviors in order to emphasize the educational content-related experiences in the learner experience stage. Second, in order for learners to easily recognize main interaction objects in order to acquire information on safe behavior procedures in unfamiliar environments, use of spatial UI or signifiers using arrows or symbols, posts that specifically instruct actions, and multisensory signals Therefore, it was found to be important to emphasize essential actions in a way that lowers the degree of freedom of user experience, and the proportion of non-realistic interactions for cognitive interactions was found to increase. Lastly, in the confirmation and evaluation stage of the experience, it is important to use the meta UI to alleviate negative experiences such as physical damage after experiencing a safety accident situation,