• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.81.2-82
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• 2003

Plants have evolved along with pathogens, and they have developed sophisticated defense systems against specific microorganisms to survive. G-protons are considered one of the upstream signaling components working as a key for the defense signal transduction pathway. For activation and inactivation of G-protein, GTP-biding proteins are involved. GTP -binding proteins are found in all organisms. Small GTP-binding proteins, having masses of 21 to 30kD, belong to a superfamily, often named the Ras supefamily because the founding members are encoded by human Ras genes initially discovered as cellular homologs of the viral ras oncogene. Members of this supefamily share several common structural features, including several guanine nucleotide binding domains and an effector binding domain. However, exhibiting a remarkable diversity in both structure and function. They are important molecular switches that cycle between the GDP-bound inactive form into the GTP-bound active form through GDP/GTP replacement. In addition, most GTP-binding proteins cycle between membrane-bound and cytosolic forms. such as the RAC family are cytosolic signal transduction proteins that often are involved in processing of extracellular stimuli. Plant RAC proteins are implicated in regulation of plant cell architecture secondary wall formation, meristem signaling, and defense against pathogens. But their molecular mechanisms and functions are not well known. We isolated a RacB homolog from rice to study its role of defense against pathogens. We introduced the constitutively active and the dominant negative forms of the GTP-hinging protein OsRacB into the wild type rice. The dominant negative foms are using two forms (full-sequence and specific RNA interference with RacB). Employing southern, and protein analysis, we examine to different things between the wild type and the transformed plant. And analyzing biolistic bombardment of onion epidermal cell with GFP-RacB fusion protein revealed association with the nucle.

• Journal of Biomedical Engineering Research
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• v.25 no.3
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• pp.165-169
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• 2004

In Magnetic Resonance Electrical Impedance Tomography (MREIT), we inject current through electrodes placed on the surface of a subject and measure the induced magnetic flux density distribution using an MRI scanner. This requires a constant current source whose output pulses are synchronized with MR pulse sequences. In this paper, we present a design and performance analysis of a current source used in a 3.0T MREIT system. The developed current source was tested using a saline phantom. We found that its performance is satisfactory for the current MREIT system. We suggest future improvements for better SNR(signal-to-noise ratio).

• Journal of the Korea Institute of Information and Communication Engineering
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• v.9 no.1
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• pp.22-33
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• 2005

In this paper, an efficient signal interference control technique to improve the high convergence speed of LMS algorithms is introduced in the adaptive transversal filter of DS/SS. The convergence characteristics of the proposed algorithm, whose coefficients are multiply adapted in a symbol time period by recycling the received data, is analyzed to prove theoretically the improvement of high convergence speed. According as the step-size parameter ${\mu}$ is increased, the rate of convergence of the algorithm is controlled. Also, an increase in the stop-size parameter ${\mu}$ has the effect of reducing the variation in the experimentally computed learning curve. Increasing the eigenvalue spread has the effect of controlling which is downed the rate of convergence of the adaptive equalizer. Increasing the steady-state value of the average squared error, proposed algorithm also demonstrate the superiority of signal interference control to the filter algorithm increasing convergence speed by (B+1) times due to the data-recycling LMS technique.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.3 no.2
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• pp.347-354
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• 1999

Generally, transceiver is operated to the remote control for the purpose of breading the traffic zone which is established on a mountain peak, island and building top using private line. Therefor, the remote control system of public radio station have been the very important role that is decision the quality relate to the quickness, accuracy, safety of communication on old type transceiver of SSB, VHF etc. In the case of using the only 1 private line which is exchanged voice signal with data signal had mixed or interrupted for up/down of channel, PTT control and monitoring of transmission channel and power. The up/down of channel and PTT control is according to the ASK and the data of monitoring is transfered to the FSK modulation, additional algorithm is studied on the serial protocol and traffic sequence using the MCS-51 processor in the simplex communication methode.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.10C
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• pp.787-797
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• 2008

In this paper, we propose a fast disparity estimation method considering temporal and spatial redundancy based on a dynamic programming for stereo matching. For the first step, the dynamic programming is performed to estimate disparity vectors with correlation between neighboring pixels in an image. Next, we efficiently compensate regions, which disparity vectors are not allocated, with neighboring disparity vectors assuming that disparity vectors in same object are quite similar. Moreover, in case of video sequence, we can decrease a complexity with temporal redundancy between neighboring frames. For performance comparison, we generate an intermediate-view image using the estimated disparity vector. Test results show that the proposed algorithm gives $0.8{\sim}2.4dB$-increased PSNR(peak signal to noise ratio) compared to a conventional block matching algorithm, and the proposed algorithm also gives approximately 0.1dB-increased PSNR and $48{\sim}68%$-lower complexity compared to the disparity estimation method based on general dynamic programming.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.8 no.10
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• pp.3475-3489
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• 2014

Here we present a simple flame detection method for an infrared (IR) thermal camera based real-time fire surveillance digital signal processor (DSP) system. Infrared thermal cameras are especially advantageous for unattended fire surveillance. All-weather monitoring is possible, regardless of illumination and climate conditions, and the data quantity to be processed is one-third that of color videos. Conventional IR camera-based fire detection methods used mainly pixel-based temporal correlation functions. In the temporal correlation function-based methods, temporal changes in pixel intensity generated by the irregular motion and spreading of the flame pixels are measured using correlation functions. The correlation values of non-flame regions are uniform, but the flame regions have irregular temporal correlation values. To satisfy the requirement of early detection, all fire detection techniques should be practically applied within a very short period of time. The conventional pixel-based correlation function is computationally intensive. In this paper, we propose an IR camera-based simple flame detection algorithm optimized with a compact embedded DSP system to achieve early detection. To reduce the computational load, block-based calculations are used to select the candidate flame region and measure the temporal motion of flames. These functions are used together to obtain the early flame detection algorithm. The proposed simple algorithm was tested to verify the required function and performance in real-time using IR test videos and a real-time DSP system. The findings indicated that the system detected the flames within 5 to 20 seconds, and had a correct flame detection ratio of 100% with an acceptable false detection ratio in video sequence level.

• Journal of Microbiology and Biotechnology
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• v.14 no.3
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• pp.563-569
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• 2004

The DNA sequence of the chitosanase gene (choK) from $\beta$-Proteobacterium KNU3 showed an 1,158-bp open reading frame that encodes a protein of 386 amino acids with a novel 74 signal peptide. The degenerated primers based on the partial deduced amino acid sequences from MALDI- TOF MS analyses yielded the 820 bp of the PCR product. Based on this information, double inverse PCR cloning experiments, which use the two specific sets of PCR primers rather than single set primers, identified the unknown 1.2 kb of the choK gene. Subsequently, a 1.8 kb of full choK gene was cloned from another PCR cloning experiment and it was then subcloned into pGEM T-easy and pUC18 vectors. The recombinant E. coli clone harboring recombinant pUC18 vector produced a clear halo around the colony in the glycol chitosan plates. The recombinant ChoK protein was secreted into medium in a mature form while the intracellular ChoK was produced without signal peptide cleavage. The activity staining of PAGE showed that the recombinant ChoK protein was identical to the chitosanase of wild-type. The comparison of deduced amino acid sequences of choK revealed that there is 92% identity with that of Sphingobacterium multivorum chitosanase. Judging from the conserved module in other bacterial chitosanases, chitosanase of KNU3 strain (ChoK) belongs to the family 80 of glycoside hydrolases.

• Journal of Microbiology and Biotechnology
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• v.24 no.12
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• pp.1609-1621
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• 2014

The plant pathogen Burkholderia gladioli, which has a broad host range that includes rice and onion, causes bacterial panicle blight and sheath rot. Based on the complete genome sequence of B. gladioli BSR3 isolated from infected rice sheaths, the genome of B. gladioli BSR3 contains the luxI/luxR family of genes. Members of this family encode N-acyl-homoserine lactone (AHL) quorum sensing (QS) signal synthase and the LuxR-family AHL signal receptor, which are similar to B. glumae BGR1. In B. glumae, QS has been shown to play pivotal roles in many bacterial behaviors. In this study, we compared the QS-dependent gene expression between B. gladioli BSR3 and a QS-defective B. gladioli BSR3 mutant in two different culture states (10 and 24 h after incubation, corresponding to an exponential phase and a stationary phase) using RNA sequencing (RNA-seq). RNA-seq analyses including gene ontology and pathway enrichment revealed that the B. gladioli BSR3 QS system regulates genes related to motility, toxin production, and oxalogenesis, which were previously reported in B. glumae. Moreover, the uncharacterized polyketide biosynthesis is activated by QS, which was not detected in B. glumae. Thus, we observed not only common QS-dependent genes between B. glumae BGR1 and B. gladioli BSR3, but also unique QS-dependent genes in B. gladioli BSR3.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.9A
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• pp.755-761
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• 2005

In this paper, we proposed a channel estimation method by impulse signal train in OFDM. In order to estimate the channel response, 4 impulse signals are generated and transmitted during one OFDM (Orthogonal Frequency Division Multiplexing) symbol. The intervals between the impulse signals are all equal in time domain. At the receiver, the impulse response signals are summed and averaged. And then, the averaged impulse response signal is zero padded and fast Fourier transformed to obtain the channel estimation. The BER performance of the proposed method is compared with those of conventional estimation method using the long training sequence in fast fading environments. The simulation results show that the proposed method improves by 3 dB in terms of Eb/No, compared with the conventional method.

• Fisheries and Aquatic Sciences
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• v.6 no.4
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• pp.180-186
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• 2003

The full-length cDNA encoding the pre-protein growth hormone (sfGH) from spotted flounder (Verasper variegatus) was amplified by the rapid amplification of cDNA ends (RACE) using degenerated oligonucleotide primers derived from conserved growth hormone sequences. It consists of 901 nucleotides in length, including the coding region of 609 nucleotides, 111 nucleotides of a 5' untranslated region, and 181 nucleotides of a 3' untranslated region. The conserved polyadenylation signal (AATAAA) lies 12 bases upstream from the poly (A) tail. The deduced amino acid sequence shows an open reading frame encoding a pre-protein of 203 amino acids and a putative signal peptide of 17 amino acids, suggesting that the mature hormone consists of 186 amino acids. The analyses of sfGH reveal some unique structural features. The repetitive sequences are located in the 5' untranslated region of sfGH cDNA and consist of tandem arrays of imperfect direct repeat monomers. Moreover, sfGH contains six Cys residues, as opposed to four or five in other GHs, and it is clearly distinguishable from olive flounder (Paralichthys olivaceus) GH, which lacks a region corresponding to residues 175-188 in alignment positions. It has important implications from an evolutionary standpoint, suggesting possible divergence among flatfishes.