Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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The Spotted Flounder (Verasper variegatus) Growth Hormone cDNA and Its Evolutionary Implications

  • Lee Jeong-Ho (Biotechnology Research Center, National Fisheries Research and Development Institute(NFRDI)) ;
  • Lee Sang-Jun (Biotechnology Research Center, National Fisheries Research and Development Institute(NFRDI)) ;
  • Kim Kyung-Kil (Biotechnology Research Center, National Fisheries Research and Development Institute(NFRDI)) ;
  • Kim Woo-Jin (Biotechnology Research Center, National Fisheries Research and Development Institute(NFRDI)) ;
  • Park Doo-Won (Biotechnology Research Center, National Fisheries Research and Development Institute(NFRDI)) ;
  • Park Jung-Youn (Biotechnology Research Center, National Fisheries Research and Development Institute(NFRDI))
  • Published : 2003.12.01
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Abstract

The full-length cDNA encoding the pre-protein growth hormone (sfGH) from spotted flounder (Verasper variegatus) was amplified by the rapid amplification of cDNA ends (RACE) using degenerated oligonucleotide primers derived from conserved growth hormone sequences. It consists of 901 nucleotides in length, including the coding region of 609 nucleotides, 111 nucleotides of a 5' untranslated region, and 181 nucleotides of a 3' untranslated region. The conserved polyadenylation signal (AATAAA) lies 12 bases upstream from the poly (A) tail. The deduced amino acid sequence shows an open reading frame encoding a pre-protein of 203 amino acids and a putative signal peptide of 17 amino acids, suggesting that the mature hormone consists of 186 amino acids. The analyses of sfGH reveal some unique structural features. The repetitive sequences are located in the 5' untranslated region of sfGH cDNA and consist of tandem arrays of imperfect direct repeat monomers. Moreover, sfGH contains six Cys residues, as opposed to four or five in other GHs, and it is clearly distinguishable from olive flounder (Paralichthys olivaceus) GH, which lacks a region corresponding to residues 175-188 in alignment positions. It has important implications from an evolutionary standpoint, suggesting possible divergence among flatfishes.

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References

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